CN201501859U - Fungal microculture device capable of being directly observed under microscope - Google Patents

Fungal microculture device capable of being directly observed under microscope Download PDF

Info

Publication number
CN201501859U
CN201501859U CN 200920034535 CN200920034535U CN201501859U CN 201501859 U CN201501859 U CN 201501859U CN 200920034535 CN200920034535 CN 200920034535 CN 200920034535 U CN200920034535 U CN 200920034535U CN 201501859 U CN201501859 U CN 201501859U
Authority
CN
China
Prior art keywords
box
box body
fungal
inner box
fungi
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 200920034535
Other languages
Chinese (zh)
Inventor
黄小军
张瑞国
曲萍
陈宝莹
于军
梁向艳
铁茹
刘芳娥
赵玉峰
刘利兵
陈健康
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fourth Military Medical University FMMU
Original Assignee
Fourth Military Medical University FMMU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fourth Military Medical University FMMU filed Critical Fourth Military Medical University FMMU
Priority to CN 200920034535 priority Critical patent/CN201501859U/en
Application granted granted Critical
Publication of CN201501859U publication Critical patent/CN201501859U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/04Flat or tray type, drawers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means

Landscapes

  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model discloses a fungal microculture device capable of being directly observed under a microscope, which is characterized by comprising a box body. A coverless inner box is arranged inside the box body, a peripheral through groove is formed between the inner box and the box body, sterile water is disposed inside the through groove, and a movable transparent cover arranged on the box body can cover or open the box body and the inner box. The fungal microculture device is capable of completing relatively good fungal microculture and fast morphological identification and resolving problems of existing experiments that procedures are trivial, culture mediums are easy to be dry and oxygen is deficient, experiment materials can not be used again after a single use and the like.

Description

Can be the fungi of microscopically direct viewing little culture apparatus
Technical field
The utility model relates to a kind of preclinical medicine experimental installation, and particularly a kind of being used for is convenient to the device observed again to the little cultivation of fungi.
Background technology
The experiment in the preclinical medicine field at present for microorganism, mostly little cultivation at fungi is to adopt on the cultivation slide with the bonding steel ring of paraffin, paper clip, the perfusion culture base is realized (Fig. 1) therein then, and the shortcoming of this method is to buy the steel ring difficulty or make time-consuming, the effort of suitable paper clip.Finish the little culture experiment complex steps of fungi, careless slightly, very easily cause environmental pollution.And, because it is few to cultivate employed substratum quantity in the traditional method, oxygen amount in the growing space that is provided is very limited, along with the prolongation of culture cycle, substratum is dry easily, and anoxic takes place the fungi of being cultivated, thereby cause fungal growth slow, biological character is not true to type, and has delayed the process of its evaluation, has influenced normally carrying out of experiment.In addition, because the related slide of the little cultivation carried out of traditional mode is tiny, frangible, link is comparatively complicated, the related article of influence recycled after cultivation finished, may cause the waste of manpower, financial resources, increase experimental cost virtually, make accuracy rate, the success ratio of experimental result all be difficult to be guaranteed.
Summary of the invention
The purpose of this utility model provide a kind of be used for the little cultivation of fungi and can be at any time at the device of microscopically direct viewing, can better finish little cultivation of fungi and identification of morphology rapidly, can solve that existing experiment link is loaded down with trivial details, substratum is easily dry, anoxic and experiment material such as can only disposablely use at problem.
For reaching above purpose, the utility model takes following technical scheme to be achieved:
A kind of can be the fungi of microscopically direct viewing little culture apparatus, it is characterized in that, comprise box body, be provided with a uncovered inner box in the box body, form peripheral groove between inner box and the box body, be provided with sterilized water in the groove, box body is provided with an active transparency cover box body and inner box is covered or open.
In the such scheme, be provided with box in the box that baffle in length and breadth is divided into the inner box chamber a plurality of uncovereds in the described inner box.Described box body is a rectangle, square or circular.
Advantage of the present utility model is:
1, inner box is the ready-made little culturing room of fungi, the experimenter can be examined under a microscope at any time while cultivating, can save film-making, buy copper ring or prepare suitable cultivation paper clip and melt step such as paraffin, save the experiment consumptive material, simplified experimental procedure.
2, baffle is divided into the inner box chamber in box in the box of a plurality of uncovereds in length and breadth, goes for the multiple substratum of a kind of bacterium co-cultivation in a box, thereby has saved the consumption of substratum greatly.
3, in box, also can place independently dismountable cell (fungies that interior cultivation is different) in the box and be convenient to observe, convenient moving.
4, the peripheral groove that forms between inner box and the box body has well solved substratum exsiccant problem thereby can inject sterilized water, has guaranteed the fungal organism proterties typical case who is cultivated.
5, compare with traditional method, the utility model device has increased the fungi growth space, guarantees that fungi grows under aerobic environment.Therefore,,, saved experimental expenses, reduced experimental cost owing to can improve accuracy rate, the success ratio of experiment increasing work efficiency and the work quality while.
Description of drawings
The utility model is described in further detail below in conjunction with drawings and the specific embodiments.
Fig. 1 is the structural representation of existing fungus culture sheet.
Fig. 2 is a kind of embodiment of the present utility model.
Fig. 3 is another kind of embodiment of the present utility model.
Among Fig. 1 to Fig. 3: 1, cultivate slide; 2, steel ring or paper clip.3, transparency cover; 4, inner box; 5, box body; 6, groove; 7, fungus culture basic unit; 8, baffle in length and breadth.
Embodiment
Embodiment 1
As shown in Figure 2, a kind of can be the fungi of microscopically direct viewing little culture apparatus, comprise box body 5, be provided with a uncovered inner box 4 in the box body 5, form peripheral groove 6 between inner box 4 and the box body 5, be filled with sterilized water in the groove to avoid substratum lack of water drying.Box body 5 one side tops are provided with an active transparency cover 3 box body 5 and inner box 4 are covered or open.The size of box is: wide by 25 * long 75mm * high 15mm.During use in uncovered inner box 4 direct a kind of fungus culture of perfusion basic unit 7, the experimenter can examine under a microscope when cultivating at any time.
Embodiment 2
As shown in Figure 3, another kind can be the fungi of microscopically direct viewing little culture apparatus, primary structure is with embodiment 1.Difference in inner box 4, be provided with cruciform in length and breadth baffle 8 the inner box chamber is divided into box in the box of four uncovereds, in each box, can pour into fungus culture basic unit 7 simultaneously in the box, applicable to the multiple substratum of a kind of bacterium co-cultivation in a box.
Embodiment 3
On the basis of embodiment 2, present embodiment also can directly not pour into earlier fungus culture basic unit 7 in the box in box, but shape is placed in each box in the box with independently dismountable uncovered capsule (fungies that interior cultivation is different) of the box in box of box, undersized in the box, both be convenient to observe, can have conveniently moved again.
The utility model is not limited to above embodiment, can make rectangle, square or circular as box body 5.The shape of corresponding inner box 4, transparency cover 3 also changes with box body 5 one.

Claims (3)

1. an energy is at the little culture apparatus of fungi of microscopically direct viewing, it is characterized in that, comprise box body, be provided with a uncovered inner box in the box body, form peripheral groove between inner box and the box body, be provided with sterilized water in the groove, box body is provided with an active transparency cover box body and inner box is covered or open.
2. as claimed in claim 1 can be the fungi of microscopically direct viewing little culture apparatus, it is characterized in that, be provided with box in the box that baffle in length and breadth is divided into the inner box chamber a plurality of uncovereds in the described inner box.
3. as claimed in claim 1 can be the fungi of microscopically direct viewing little culture apparatus, it is characterized in that described box body is a rectangle, square or circular.
CN 200920034535 2009-09-15 2009-09-15 Fungal microculture device capable of being directly observed under microscope Expired - Fee Related CN201501859U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200920034535 CN201501859U (en) 2009-09-15 2009-09-15 Fungal microculture device capable of being directly observed under microscope

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200920034535 CN201501859U (en) 2009-09-15 2009-09-15 Fungal microculture device capable of being directly observed under microscope

Publications (1)

Publication Number Publication Date
CN201501859U true CN201501859U (en) 2010-06-09

Family

ID=42452796

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200920034535 Expired - Fee Related CN201501859U (en) 2009-09-15 2009-09-15 Fungal microculture device capable of being directly observed under microscope

Country Status (1)

Country Link
CN (1) CN201501859U (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102533930A (en) * 2012-01-05 2012-07-04 中国海洋大学 Manufacturing method of slide for observing filamentous fungus microscopic morphology
WO2020198326A1 (en) * 2019-03-26 2020-10-01 Xona Microfluidics, Inc. Apparatus and method for humidifying cell cultures
CN113455278A (en) * 2021-06-29 2021-10-01 江苏省农业科学院 Identification device and identification method for anti-Tyrophagus putrescentiae of oyster mushroom

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102533930A (en) * 2012-01-05 2012-07-04 中国海洋大学 Manufacturing method of slide for observing filamentous fungus microscopic morphology
CN102533930B (en) * 2012-01-05 2014-04-09 中国海洋大学 Manufacturing method of slide for observing filamentous fungus microscopic morphology
WO2020198326A1 (en) * 2019-03-26 2020-10-01 Xona Microfluidics, Inc. Apparatus and method for humidifying cell cultures
CN113455278A (en) * 2021-06-29 2021-10-01 江苏省农业科学院 Identification device and identification method for anti-Tyrophagus putrescentiae of oyster mushroom
CN113455278B (en) * 2021-06-29 2023-03-07 江苏省农业科学院 Identification device and identification method for anti-Tyrophagus putrescentiae of oyster mushroom

Similar Documents

Publication Publication Date Title
CN201501859U (en) Fungal microculture device capable of being directly observed under microscope
CN204644371U (en) A kind of integrated form many cells noncontact co-culture device
CN100560710C (en) High-density array type cell slide culture apparatus, storage facility and experimental installation
CN102533930B (en) Manufacturing method of slide for observing filamentous fungus microscopic morphology
CN2863808Y (en) Cell multiple-grooved incubator
CN101665767A (en) High-density array type cell growth cover glass experimental device
CN203144384U (en) Illumination fermentation pot
CN102703567B (en) Multi-phase co-culture and detection method and device thereof
CN105886380B (en) A kind of Petri dish box
CN204752694U (en) Burnt piece of cultivateing of copolymerization
CN202576404U (en) Experiment device for anaerobic microorganism culture
CN104164365A (en) In-vitro cell contact type co-culture device and culture operation method thereof
CN214088518U (en) Strain culture tank for producing efficient microbial fertilizer
CN204125459U (en) A kind of biological culture laboratory apparatus being convenient to observe
CN204589016U (en) A kind of miniature aerobic composting device
CN205856468U (en) Tissue Culture Dish
CN207130275U (en) A kind of co-culture device
CN205077033U (en) Device of screening microorganism
CN207294805U (en) A kind of cell co-cultures bottle
CN205635399U (en) Many functional microbes fertilizer processing equipment
CN203846033U (en) Combined type preparation device of double-sided solid culture medium
CN204138673U (en) A kind of culture dish and culturing bottle dual-purpose apparatus
CN203399586U (en) Peony tissue culture flask
CN204918601U (en) Special box of incubator
CN204848853U (en) Light bioreaction system

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20100609

Termination date: 20110915