CN101665767A - High-density array type cell growth cover glass experimental device - Google Patents
High-density array type cell growth cover glass experimental device Download PDFInfo
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- CN101665767A CN101665767A CN200910158638A CN200910158638A CN101665767A CN 101665767 A CN101665767 A CN 101665767A CN 200910158638 A CN200910158638 A CN 200910158638A CN 200910158638 A CN200910158638 A CN 200910158638A CN 101665767 A CN101665767 A CN 101665767A
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Abstract
The invention discloses a culture device, a storage device and an experimental device for high-density array type cell growth cover glass. The culture device is provided with a slide plate (1), slotsin ordered arrangement are arranged on the slide plate (1), the depth of each slot (2) is 0.3-0.4mm, and a slide (3) which is matched with the bottom shape of the slot (2) is arranged in the slot (2).The storage device is provided with a bracket (6) which is provided with a plurality of openings (7) which are in ordered arrangement and are used for placing a round growth cover glass, the length of the opening (7) is smaller than the diameter of the round slide (3), the middle width of the opening (7) is bigger than that of two ends, and the width of two ends is 0.15-0.18mm. The experimental device is an experimental plate (8) which is provided with a plurality of grooves (9) which are in ordered arrangement and are used for placing the growth cover glass, the shape of the cross section ofthe groove (9) is matched with the shape of the slide (3), and the depth is 4-6mm. The invention has the advantages of convenient use, high efficiency, favourable identity and the like.
Description
The present invention is that patent application day 20060831, application number are 200610047607.0, and invention and created name is divided an application for " high-density array type cell slide culture apparatus, storage facility and experimental installation ".
Technical field:
The present invention relates to a kind of cell culture apparatus, especially a kind of easy to use, efficient is high, the high-density array type cell slide culture apparatus of favourable identity, storage facility and experimental installation.
Background technology:
Cell in vitro is cultivated the influence of having simplified environmental factors, having got rid of some complicated interfering factorss of experiment in the body, helps using the rule of extraneous processing factor, exploration and announcement cellular activities such as various physics and chemistry and biology; Cell in vitro is cultivated and is convenient to adopt various technology and method, analyzes and observes under normal and altered life condition, variation and the intrinsic mechanism of cell on form and function; Particularly with the people during as research object, it is not only economical convenient that cell in vitro is cultivated, but also avoided the problem in many ethics to a great extent.Therefore, cell in vitro is cultivated to have become and is comprised one of experimental technique the most commonly used that biomedical each research field of life science is indispensable.
Along with constantly enriching of life science content is abundant, the technical indicator of utilizing the cell culture experiments sample to analyze and observe also is on the increase, and the field of containing enlarges day by day.But in each test, cell itself and culture condition thereof can change to some extent, and therefore each allogenic cell of cultivating will be different at aspects such as growth pattern, cell and molecular biological characteristic, causes experimental result to produce deviation.For avoiding above-mentioned situation to take place, people generally use a kind of external cell culture method of improvement at present, that is: get some suitable sizes (as the cover glass of 10mm * 20mm, thickness 0.14.～0.17mm), pickling and abundant flushing back autoclave sterilization; Cover glass is put into culture dish/plate, conventional cell is laid on sheet before; When cell after covering with substantially on the cover glass, sheet is taken out; 4 ℃ down with PBS rinsing 3 times, fully remove remaining substratum and serum, cell faces up and puts into 4 ℃ of cold acetones and fix 20 minutes, after drying cover glass is put into culture dish or other container ,-20 ℃ of preservations are standby.But this kind cell climbing sheet cultural method exists following problem:
1. cover glass moves in slick bottle ware bottom easily and is overlapping, the cover glass that has because of overlapping generation slit at two-sided grown cell, thereby make growth conditions and the area of cell on every cover glass all different,
Existing because of the identity of creep plate is bad causes experimental result to produce the problem of deviation; For fear of this problem, the experimenter arranges cover glass loose usually, and the cell climbing sheet quantity of the each preparation of result is very limited, generally has only 7～10, is difficult to satisfy the needs of multi-level, many index researchs;
2. do not have special-purpose creep plate storage facility, creep plate is preserved under-20 ℃ of conditions when standby, not only take more reefer space, cause energy dissipation, and the creep plate of at every turn taking bothers very much, and is careless slightly, will make the creep plate damage;
3. do not have special-purpose creep plate experimental installation, each experiment all is that creep plate directly is shelved on the vessel bottom surface, and is container with vessel, the soup that interpolation can be submerged creep plate, and the soup demand is big; Because the surface-area of vessel is big, the experimentation herb liquid evaporates easily, not only wastes soup, but also is difficult to obtain experimental result accurately simultaneously; Each when changing different soup, all need creep plate is taken out to dry upper and lower surface, the experimental implementation step is very loaded down with trivial details, efficient is low.
Summary of the invention:
The present invention is in order to solve existing in prior technology the problems referred to above, provide a kind of easy to use, efficient is high, the high-density array type cell slide culture apparatus of favourable identity, storage facility and experimental installation.
Technical solution of the present invention is: a kind of high-density array type cell slide culture apparatus, it is characterized in that: slide glass plate 1 is arranged, be provided with the groove 2 of a plurality of ordered arrangement on slide glass plate 1, the degree of depth of groove 2 is 0.3～0.4mm, and groove 2 is built-in with the slide 3 that matches with groove 2 bottom shapes.
The top both sides of described slide glass plate 1 are provided with handle 4.
Described groove 2 is circular and square.
On the right side wall of described groove 2, be provided with and get sheet breach 5.
A kind of high-density array type cell slide storage facility, it is characterized in that: support 6 is arranged, support 6 is provided with the opening 7 that is used to place circular creep plate of a plurality of ordered arrangement, the length of opening 7 is less than the diameter of circular slide 3, the intermediate width of opening 7 is greater than the two ends width, and the two ends width is 0.15～0.18mm.
A kind of high-density array type cell growth cover glass experimental device is characterized in that: brassboard 8 is arranged, and brassboard 8 is provided with the groove 9 that is used to place creep plate of a plurality of ordered arrangement, and the shape of cross section of groove 9 and slide 3 shapes match, and the degree of depth is 4～6mm.
The present invention compares with prior art, has following advantage:
1. can in the culture dish of diameter 10cm, obtain 50～100 cell climbing sheets that grow fine, cell climbing sheet culture efficiency height simultaneously;
2. because slide is embedded in the groove on the slide glass plate, and it is overlapping that it is difficult for, the growth of cell single face is for follow-up test provides great convenience;
3. the degree of depth of groove only exceeds slide thickness 0.13～0.26mm, therefore the cell culture fluid on each slide is in same plane, cell on each slide is grown under same culture conditions, thereby guaranteed the identity of creep plate, the collimation and the stability of experimental result;
4. the handrail that is provided with on the slide glass plate can take, put the slide glass plate in culture dish very easily, and be provided with on the groove right side wall get the sheet breach, also can easily slide be put into and creep plate is taken out groove, operation is simple;
5. the creep plate of same size vertically can be arranged in an orderly manner in the special-purpose storage facility, can save a large amount of reefer spaces, reach the purpose of save energy afterwards; The opening setting that wide both sides, centre in the storage facility are narrow, make things convenient for creep plate placement, take, effectively prevent because of the damage of misoperation creep plate;
6. creep plate is placed on the special-purpose experimental installation, be about to creep plate and place groove (operation cell) on the brassboard, related experiment steps such as subsequently immunohistochemical methods, in situ hybridization, immunofluorescence are carried out with the form of efficient quick.The coupling of cell and creep plate is coincide and is provided with, only soup (50 μ l) being splashed into groove submerges creep plate and gets final product, can save a large amount of soups, simultaneously can utilize the cell of relative closure to avoid liquid evaporation (dry plate) again, help obtaining the really reliable experimental result in Huaihe River, will creep plate in experimentation do not take out repeatedly and dry, simplified operation steps greatly.
In a word, the present invention helps to obtain a large amount of uniform and stable cell climbing sheets, and experimental implementation is simple, has avoided the experimental bias and the error that are caused by operation to a great extent, make cell culture technology in the life science field, bring into play bigger effect, have quite wide application prospect.
Description of drawings:
Fig. 1 is the structural representation of the embodiment of the invention 1.
Fig. 2 is the A-A view of Fig. 1.
Fig. 3 is the structural representation of the embodiment of the invention 2.
Fig. 4 is the C-C view of Fig. 2.
The structural representation of Fig. 5 embodiment of the invention 3.
Fig. 6 is the structural representation of embodiment of the invention storage facility.
Fig. 7 is the structural representation of embodiment of the invention experimental installation.
Fig. 8 is the B-B view of Fig. 7.
Embodiment:
Below in conjunction with description of drawings the specific embodiment of the present invention.
Embodiment 1 is as shown in Figure 1, 2:
Have than the circular mount plate 1 of cultivating the little 2mm of vessel diameter, slide glass plate 1 usefulness can autoclaved silica gel, metal or other material are made.Both sides are provided with 0.5～0.8 high handle 4 above the slide glass plate 1, are convenient to picking and placeing of slide glass plate 1.On slide glass plate 1, be provided with ordered arrangement to recessed circular groove 2, the degree of depth of circular groove 2 is 0.3～0.4mm, circular groove 2 is built-in with the circular slide 3 that coincide with groove 2 bottom mating, and it is the standard slide of 0.14～0.17mm that slide 3 adopts thickness, is provided with on the right side wall of groove 2 and gets sheet breach 5.In order to distinguish the positive and negative of creep plate, can on slide, make sign, go out " R " letter as front with mill (spray) sand milling at slide.
Embodiment 1 can be independent platy structure, places Tissue Culture Dish during use; Also the bottom of Tissue Culture Dish can be made the structure formation of present embodiment 1.
Cultured high-density array type cell slide can be placed on the storage facility as shown in Figure 6 and handle, be directly used in experiment afterwards or be placed under-20 ℃ of conditions preserve standby.Useful acidproof, the alkali proof rigid plastics of described storage facility or other material are made support 6, support 6 is provided with the opening 7 that is used to place circular creep plate of a plurality of ordered arrangement, the length of opening 7 is less than the diameter of circular slide 3, the intermediate width of opening 7 is greater than the two ends width, the two ends width is 0.15～0.18mm, and 1/4～1/3 of circular creep plate lower end is placed in the opening.
When the various experiment of the immunohistochemical staining that carries out cell climbing sheet, DNA and RNA in situ hybridization and immunofluorescence etc., creep plate can be placed on the experimental installation shown in Fig. 7,8.Described experimental installation has brassboard 8, brassboard 8 is provided with the groove 9 that is used to place creep plate of a plurality of ordered arrangement, the shape of cross section of groove 9 and slide 3 shapes are complementary identical, as the creep plate diameter is 6mm, the diameter of groove 9 can be 0.65mm, the degree of depth is 4～6mm, make experimental implementation save time more, laborsaving, economize medicine, obtain the experimental result of science more.Equally, experimental installation can be independent platy structure, places vessel with cover during use; Also the bottom of vessel with cover can be made the structure formation of experimental installation.
Concrete operation method is:
1., do and do roasting the processing after 2 hours with slide glass plate 1 autoclaving or with alcohol-pickled 0.5 hour and uv irradiating;
2. adopt and handle slide 3 in a like fashion;
3. in super clean bench, slide is inserted seriatim each groove 2 of slide glass plate; After piling fully, the slide glass plate 1 that is loaded with slide 3 is put into the culture dish bottom;
4. be routinely added to culturing cell; After reaching predetermined state, creep plate is put on the experimental installation successively, promptly in the opening 7 on the support 6, wholely immerses among the PBS washing 3 times, fix 20 minutes in cold acetone, after fully drying, be directly used in experiment purpose or seal ,-20 ℃ of preservations are stand-by;
5. cell climbing sheet is put on the experimental installation one by one, promptly in the groove 9 on the brassboard 8, groove 9 holds its cell climbing sheet just, and experimental procedure detects routinely;
6. operator's console is added a cover in the experimentation, to prevent liquid evaporation;
7. after the experiment, on slide glass with the density of 6～10 cell climbing sheets mounting successively;
8. mirror is observed down.
Other is with embodiment 1, and just groove 2 is surrounded by silica gel plate and the bulge loop on it 10, gets sheet breach 5 and is arranged on the bulge loop 10.
Claims (1)
1. high-density array type cell growth cover glass experimental device, it is characterized in that: brassboard (8) is arranged, brassboard (8) is provided with the groove that is used to place creep plate (9) of a plurality of ordered arrangement, and the shape of cross section of groove (9) and slide (3) shape match, and the degree of depth is 4～6mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910158638A CN101665767A (en) | 2006-08-31 | 2006-08-31 | High-density array type cell growth cover glass experimental device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910158638A CN101665767A (en) | 2006-08-31 | 2006-08-31 | High-density array type cell growth cover glass experimental device |
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| CNB2006100476070A Division CN100560710C (en) | 2006-08-31 | 2006-08-31 | High-density array type cell slide culture apparatus, storage facility and experimental installation |
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| CN101665767A true CN101665767A (en) | 2010-03-10 |
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| CN200910158638A Pending CN101665767A (en) | 2006-08-31 | 2006-08-31 | High-density array type cell growth cover glass experimental device |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206582A (en) * | 2010-09-06 | 2011-10-05 | 李宏 | Slide fixing mount for high-density array type cell climbing sheet culture dish |
| CN102925354A (en) * | 2012-11-06 | 2013-02-13 | 无锡耐思生物科技有限公司 | Slide culture dish used by slides in two specifications in combination mode |
| CN102943037A (en) * | 2012-10-30 | 2013-02-27 | 无锡耐思生物科技有限公司 | Multi-hole cell culture dish structure |
| CN106754368A (en) * | 2016-12-15 | 2017-05-31 | 广州洁特生物过滤股份有限公司 | A kind of manufacture method of cell culture apparatus |
| CN107941591A (en) * | 2017-12-28 | 2018-04-20 | 贵州大学 | Small-sized mite class cleaning device |
| WO2018107435A1 (en) * | 2016-12-15 | 2018-06-21 | 广州洁特生物过滤股份有限公司 | Coverslip for cell culture and cell culture device |
| CN109294915A (en) * | 2018-10-15 | 2019-02-01 | 郝佳琪 | A kind of zooblast experiment is combined with slide |
-
2006
- 2006-08-31 CN CN200910158638A patent/CN101665767A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206582A (en) * | 2010-09-06 | 2011-10-05 | 李宏 | Slide fixing mount for high-density array type cell climbing sheet culture dish |
| CN102943037A (en) * | 2012-10-30 | 2013-02-27 | 无锡耐思生物科技有限公司 | Multi-hole cell culture dish structure |
| CN102943037B (en) * | 2012-10-30 | 2014-09-17 | 无锡耐思生物科技有限公司 | Multi-hole cell culture dish structure |
| CN102925354A (en) * | 2012-11-06 | 2013-02-13 | 无锡耐思生物科技有限公司 | Slide culture dish used by slides in two specifications in combination mode |
| CN106754368A (en) * | 2016-12-15 | 2017-05-31 | 广州洁特生物过滤股份有限公司 | A kind of manufacture method of cell culture apparatus |
| WO2018107435A1 (en) * | 2016-12-15 | 2018-06-21 | 广州洁特生物过滤股份有限公司 | Coverslip for cell culture and cell culture device |
| CN108495923A (en) * | 2016-12-15 | 2018-09-04 | 广州洁特生物过滤股份有限公司 | A kind of cell climbing sheet and cell culture apparatus |
| CN106754368B (en) * | 2016-12-15 | 2019-07-23 | 广州洁特生物过滤股份有限公司 | A kind of manufacturing method of cell culture apparatus |
| CN108495923B (en) * | 2016-12-15 | 2021-09-10 | 广州洁特生物过滤股份有限公司 | Cell climbing sheet and cell culture device |
| CN107941591A (en) * | 2017-12-28 | 2018-04-20 | 贵州大学 | Small-sized mite class cleaning device |
| CN109294915A (en) * | 2018-10-15 | 2019-02-01 | 郝佳琪 | A kind of zooblast experiment is combined with slide |
| CN109294915B (en) * | 2018-10-15 | 2024-01-02 | 郝佳琪 | Slide combination for animal cell experiment |
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Open date: 20100310 |