MXPA02006093A - Embryonic or stemlike cells produced by cross species nuclear transplantation. - Google Patents

Embryonic or stemlike cells produced by cross species nuclear transplantation.

Info

Publication number
MXPA02006093A
MXPA02006093A MXPA02006093A MXPA02006093A MXPA02006093A MX PA02006093 A MXPA02006093 A MX PA02006093A MX PA02006093 A MXPA02006093 A MX PA02006093A MX PA02006093 A MXPA02006093 A MX PA02006093A MX PA02006093 A MXPA02006093 A MX PA02006093A
Authority
MX
Mexico
Prior art keywords
cells
embryonic
donor
cell
nuclear transfer
Prior art date
Application number
MXPA02006093A
Other languages
Spanish (es)
Inventor
Robert Lanza
Original Assignee
Univ Massachusetts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Massachusetts filed Critical Univ Massachusetts
Publication of MXPA02006093A publication Critical patent/MXPA02006093A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell, in conjunction with compatible cytoplasm andor mitochondria (of same species as donor), are provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stemlike cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation. Nuclear transfer efficiency is enhanced by introduction of compatible cytoplasm or mitochondrial DNA (same species or similar to donor cell or nucleus). Also, nuclear transfer efficiency can further optionally be enhanced by genetically altering donor cells to inhibit apoptosis, by selecting for a specific cell cycle andor by culturing under conditions that enhance embryonic growth and development.
MXPA02006093A 1999-12-20 2000-05-10 Embryonic or stemlike cells produced by cross species nuclear transplantation. MXPA02006093A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46707699A 1999-12-20 1999-12-20
PCT/US2000/012631 WO2001046401A1 (en) 1999-12-20 2000-05-10 Embryonic or stem-like cells produced by cross species nuclear transplantation

Publications (1)

Publication Number Publication Date
MXPA02006093A true MXPA02006093A (en) 2004-08-23

Family

ID=23854253

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02006093A MXPA02006093A (en) 1999-12-20 2000-05-10 Embryonic or stemlike cells produced by cross species nuclear transplantation.

Country Status (10)

Country Link
EP (1) EP1240318A4 (en)
JP (1) JP2004500066A (en)
CN (1) CN1425064A (en)
AU (1) AU784731B2 (en)
BR (1) BR0016561A (en)
CA (1) CA2394614A1 (en)
IL (2) IL150065A0 (en)
MX (1) MXPA02006093A (en)
NZ (1) NZ519346A (en)
WO (1) WO2001046401A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7696404B2 (en) 1996-08-19 2010-04-13 Advanced Cell Technology, Inc. Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
IL147179A0 (en) * 1999-06-30 2002-08-14 Advanced Cell Tech Inc Cytoplasmic transfer to de-differentiate recipient cells
JP2003525031A (en) * 1999-09-14 2003-08-26 ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス Method for enhancing embryo development by genetically modifying embryonic cells or stem-like cell lines and donor cells produced by hybrid nuclear transfer or tissue culture conditions
AUPR247901A0 (en) * 2001-01-10 2001-02-01 Garelag Pty Ltd Activation of nuclear transfer embryos
AU2002313817A1 (en) 2001-08-27 2003-03-10 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
AU2003211801A1 (en) * 2002-02-27 2003-09-09 Horri, Takuro Embryonically modified animal and method of constructing the same
EP1633768A2 (en) * 2003-05-13 2006-03-15 The Regents Of The University Of Colorado Diagnostic and therapeutic treatments related to mitochondrial disorders
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
EP2479256A1 (en) 2004-11-04 2012-07-25 Advanced Cell Technology, Inc. Derivation of embryonic stem cells
EP1984487B1 (en) 2005-08-03 2022-10-12 Astellas Institute for Regenerative Medicine Improved methods of reprogramming animal somatic cells
US8372644B2 (en) * 2006-02-06 2013-02-12 Transplantation Limited Method for growing adult cells
EP3190178A1 (en) 2007-02-23 2017-07-12 Astellas Institute for Regenerative Medicine Highly efficient methods for reprogramming differentiated cells and for generating animals and embryonic stem cells from reprogrammed cells
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
CN107889507B (en) 2015-03-23 2021-11-26 安斯泰来再生医药协会 Improved potency assay for human Retinal Pigment (RPE) cells and photoreceptor progenitors

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5061620A (en) * 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
CN1230989A (en) * 1996-08-19 1999-10-06 马萨诸塞大学 Embryonic or stem-like cell lines produced by cross species nuclear transplantation
WO1999005266A2 (en) * 1997-07-26 1999-02-04 Wisconsin Alumni Research Foundation Trans-species nuclear transfer
IL138201A0 (en) * 1998-03-02 2001-10-31 Univ Massachusetts Embryonic or stem-like cell lines produced by cross-species nuclear transplantation
GB9809178D0 (en) * 1998-04-29 1998-07-01 Univ Edinburgh Nuclear reprogramming of somatic cells
EP1109890A4 (en) * 1999-06-30 2004-12-29 Hwang Woo Suk Method for producing human cloned embryos by employing inter-species nuclear transplantation technique
JP2003525031A (en) * 1999-09-14 2003-08-26 ユニバシティ オブ マサチューセッツ、ア パブリック インスチチユーション オブ ハイアー エデュケイション オブ ザ コモンウエルス オブ マサチューセッツ、アズ リプリゼンテッド バイ イッツ アマースト キャンパス Method for enhancing embryo development by genetically modifying embryonic cells or stem-like cell lines and donor cells produced by hybrid nuclear transfer or tissue culture conditions

Also Published As

Publication number Publication date
EP1240318A1 (en) 2002-09-18
WO2001046401A8 (en) 2001-07-26
AU4830900A (en) 2001-07-03
CN1425064A (en) 2003-06-18
IL150065A (en) 2009-09-22
CA2394614A1 (en) 2001-06-28
EP1240318A4 (en) 2004-12-08
JP2004500066A (en) 2004-01-08
NZ519346A (en) 2004-02-27
WO2001046401A1 (en) 2001-06-28
IL150065A0 (en) 2002-12-01
AU784731B2 (en) 2006-06-08
BR0016561A (en) 2002-09-10

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