BR0016561A - Embryonic or similar cells to stem cells produced by cross-species nuclear transplants - Google Patents

Embryonic or similar cells to stem cells produced by cross-species nuclear transplants

Info

Publication number
BR0016561A
BR0016561A BR0016561-1A BR0016561A BR0016561A BR 0016561 A BR0016561 A BR 0016561A BR 0016561 A BR0016561 A BR 0016561A BR 0016561 A BR0016561 A BR 0016561A
Authority
BR
Brazil
Prior art keywords
cells
embryonic
similar
cell
species
Prior art date
Application number
BR0016561-1A
Other languages
Portuguese (pt)
Inventor
Jose Cibelli
Robert Lanza
James Robl
Original Assignee
Univ Massachusetts Public Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Massachusetts Public Inst filed Critical Univ Massachusetts Public Inst
Publication of BR0016561A publication Critical patent/BR0016561A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

"CéLULAS EMBRIONáRIAS OU SIMILARES A CéLULAS TRONCO PRODUZIDAS POR TRANSPLANTE NUCLEAR EM ESPéCIES CRUZADAS". é fornecido um processo para a transferência nuclear que envolve o transplante de núcleos celulares doadores diferenciados em óocitos enucleados de uma espécie diferente da célula doadora, em associação com o citoplasma e/ou as mitocóndrias compatíveis (da mesma espécie que o doador). As unidades de transferência nuclear resultantes são úteis para a produção de células similares a células tronco embrionárias isogênicas, em particular células embrionárias ou similares a células tronco isogênicas de seres humanos. Estas células embrionárias ou similares a células tronco são úteis para a produção de células diferenciadas desejadas e para a introdução, a remoção ou a modificação de genes desejados, por exemplo, em sítios específicos do genoma de tais células por recombinação homóloga. Estas células, que podem conter um gene heterólogo, são especialmente úteis em terapias de transplante de célula e para o estudo in vitro da diferenciação celular. A eficiência da transferência nuclear é melhorada por introdução de citoplasma ou DNA mitocondrial compatível (mesma espécie ou espécie similar da célula ou do núcleo doadores). Além disto, a eficiência da transferência nuclear pode também opcionalmente ser melhorada por alteração genética de células doadoras para inibir a apoptose, selecionando um ciclo celular específico e/ou cultivando sob condições que melhoram o crescimento e o desenvolvimento embrionário."EMBRYONIC CELLS OR SIMILAR TO STEM CELLS PRODUCED BY NUCLEAR TRANSPLANTATION IN CROSS-SPECIES". a process for nuclear transfer is provided which involves the transplantation of differentiated donor cell nuclei into enucleated oocytes of a different species from the donor cell, in association with the cytoplasm and / or compatible mitochondria (of the same species as the donor). The resulting nuclear transfer units are useful for the production of cells similar to isogenic embryonic stem cells, in particular embryonic cells or similar to human isogenic stem cells. These embryonic or stem cell-like cells are useful for the production of desired differentiated cells and for the introduction, removal or modification of desired genes, for example, at specific sites in the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplant therapies and for the in vitro study of cell differentiation. The efficiency of nuclear transfer is improved by introducing cytoplasm or compatible mitochondrial DNA (same species or similar species from the donor cell or nucleus). In addition, the efficiency of nuclear transfer can also optionally be improved by genetic alteration of donor cells to inhibit apoptosis, selecting a specific cell cycle and / or cultivating under conditions that improve embryonic growth and development.

BR0016561-1A 1999-12-20 2000-05-10 Embryonic or similar cells to stem cells produced by cross-species nuclear transplants BR0016561A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46707699A 1999-12-20 1999-12-20
PCT/US2000/012631 WO2001046401A1 (en) 1999-12-20 2000-05-10 Embryonic or stem-like cells produced by cross species nuclear transplantation

Publications (1)

Publication Number Publication Date
BR0016561A true BR0016561A (en) 2002-09-10

Family

ID=23854253

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0016561-1A BR0016561A (en) 1999-12-20 2000-05-10 Embryonic or similar cells to stem cells produced by cross-species nuclear transplants

Country Status (10)

Country Link
EP (1) EP1240318A4 (en)
JP (1) JP2004500066A (en)
CN (1) CN1425064A (en)
AU (1) AU784731B2 (en)
BR (1) BR0016561A (en)
CA (1) CA2394614A1 (en)
IL (2) IL150065A0 (en)
MX (1) MXPA02006093A (en)
NZ (1) NZ519346A (en)
WO (1) WO2001046401A1 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7696404B2 (en) 1996-08-19 2010-04-13 Advanced Cell Technology, Inc. Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
US7621606B2 (en) 2001-08-27 2009-11-24 Advanced Cell Technology, Inc. Trans-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
WO2001000650A1 (en) 1999-06-30 2001-01-04 Advanced Cell Technology, Inc. Cytoplasmic transfer to de-differentiate recipient cells
MXPA02002744A (en) * 1999-09-14 2003-07-21 Univ Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions.
AUPR247901A0 (en) * 2001-01-10 2001-02-01 Garelag Pty Ltd Activation of nuclear transfer embryos
KR20040099286A (en) * 2002-02-27 2004-11-26 야스미쯔 나가오 Embryonically Modified Animal and Method of Constructing the Same
EP1633768A2 (en) * 2003-05-13 2006-03-15 The Regents Of The University Of Colorado Diagnostic and therapeutic treatments related to mitochondrial disorders
EP2960328A1 (en) 2004-11-04 2015-12-30 Ocata Therapeutics, Inc. Derivation of embryonic stem cells
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
ES2836764T3 (en) 2005-08-03 2021-06-28 Astellas Inst For Regenerative Medicine Improved methods of reprogramming animal somatic cells
US8372644B2 (en) * 2006-02-06 2013-02-12 Transplantation Limited Method for growing adult cells
CN105441385A (en) 2007-02-23 2016-03-30 奥卡塔治疗公司 Effective method for generating animal and embryonic stem cells of reprogramming differentiation cells and reprogramming cells
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
CA3188112A1 (en) 2015-03-23 2016-09-29 Astellas Institute For Regenerative Medicine Improved assays for potency of human retinal pigment epithelium (rpe) cells and photoreceptor progenitors

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5061620A (en) * 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
CN1230989A (en) * 1996-08-19 1999-10-06 马萨诸塞大学 Embryonic or stem-like cell lines produced by cross species nuclear transplantation
WO1999005266A2 (en) * 1997-07-26 1999-02-04 Wisconsin Alumni Research Foundation Trans-species nuclear transfer
IL138201A0 (en) * 1998-03-02 2001-10-31 Univ Massachusetts Embryonic or stem-like cell lines produced by cross-species nuclear transplantation
GB9809178D0 (en) * 1998-04-29 1998-07-01 Univ Edinburgh Nuclear reprogramming of somatic cells
RU2216591C2 (en) * 1999-06-30 2003-11-20 Воо-Сук ХВАНГ Method for obtaining human cloned embryos due to applying the method of interspecific nuclear transplantation
MXPA02002744A (en) * 1999-09-14 2003-07-21 Univ Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions.

Also Published As

Publication number Publication date
WO2001046401A1 (en) 2001-06-28
WO2001046401A8 (en) 2001-07-26
CA2394614A1 (en) 2001-06-28
EP1240318A4 (en) 2004-12-08
MXPA02006093A (en) 2004-08-23
AU4830900A (en) 2001-07-03
NZ519346A (en) 2004-02-27
AU784731B2 (en) 2006-06-08
EP1240318A1 (en) 2002-09-18
IL150065A0 (en) 2002-12-01
JP2004500066A (en) 2004-01-08
IL150065A (en) 2009-09-22
CN1425064A (en) 2003-06-18

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A ANUIDADE

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1942 DE 25/03/2008.