WO2003057836A3 - Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions - Google Patents

Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions Download PDF

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Publication number
WO2003057836A3
WO2003057836A3 PCT/US2002/041572 US0241572W WO03057836A3 WO 2003057836 A3 WO2003057836 A3 WO 2003057836A3 US 0241572 W US0241572 W US 0241572W WO 03057836 A3 WO03057836 A3 WO 03057836A3
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WO
WIPO (PCT)
Prior art keywords
embryonic
cells
stem
methods
cell
Prior art date
Application number
PCT/US2002/041572
Other languages
French (fr)
Other versions
WO2003057836A2 (en
Inventor
Jose Cibelli
Michael West
Original Assignee
Advanced Cell Tech Inc
Jose Cibelli
Michael West
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Cell Tech Inc, Jose Cibelli, Michael West filed Critical Advanced Cell Tech Inc
Priority to CA002471827A priority Critical patent/CA2471827A1/en
Priority to AU2002367294A priority patent/AU2002367294A1/en
Publication of WO2003057836A2 publication Critical patent/WO2003057836A2/en
Publication of WO2003057836A3 publication Critical patent/WO2003057836A3/en
Priority to AU2008229989A priority patent/AU2008229989A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

An improved method of nuclear transfer involving the transplantation of differentiated donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation. Also, methods for improving nuclear transfer efficiency by genetically altering donor cells to inhibit apoptosis, select for a specific cell cycle and/or enhance embryonic growth and development are provided.
PCT/US2002/041572 2001-12-27 2002-12-27 Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions WO2003057836A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002471827A CA2471827A1 (en) 2001-12-27 2002-12-27 Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
AU2002367294A AU2002367294A1 (en) 2001-12-27 2002-12-27 Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions
AU2008229989A AU2008229989A1 (en) 2001-12-27 2008-10-17 Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US34235801P 2001-12-27 2001-12-27
US60/342,358 2001-12-27
US35784802P 2002-02-21 2002-02-21
US60/357,848 2002-02-21

Publications (2)

Publication Number Publication Date
WO2003057836A2 WO2003057836A2 (en) 2003-07-17
WO2003057836A3 true WO2003057836A3 (en) 2003-10-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/041572 WO2003057836A2 (en) 2001-12-27 2002-12-27 Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions

Country Status (3)

Country Link
AU (2) AU2002367294A1 (en)
CA (1) CA2471827A1 (en)
WO (1) WO2003057836A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2659945C (en) 2005-08-03 2014-12-16 Advanced Cell Technology, Inc. Improved methods of reprogramming animal somatic cells
CN1955738B (en) * 2005-10-28 2012-01-11 中国科学院海洋研究所 Immunofluorescence dyeing viewing method for left-eyed flounder zygote microtubule skeleton
AU2008218998A1 (en) 2007-02-23 2008-08-28 Advanced Cell Technology, Inc. Highly efficient methods for reprogramming differentiated cells and for generating animals and embryonic stem cells from reprogrammed cells
US10865383B2 (en) 2011-07-12 2020-12-15 Lineage Cell Therapeutics, Inc. Methods and formulations for orthopedic cell therapy

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEN D. ET AL.: "The giant Panda (Ailuropoda melanoleuca)somcit nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg", SCIENCE IN CHINA, vol. 42, no. 4, August 1999 (1999-08-01), pages 346 - 353, XP002967307 *
LOI P. ET AL.: "Genetic rescue of an endangered mammal by cross-species nuclear transfer using Post-Mortem somatic cells", NATURE BIOTECHNOLOGY, vol. 19, October 2001 (2001-10-01), pages 962 - 964, XP002967306 *
THOMSON J.A. ET AL.: "Embryonic stem cell lines derived from human blastocysts", SCIENCE, vol. 282, 6 November 1998 (1998-11-06), pages 1145 - 1147, XP002933311 *

Also Published As

Publication number Publication date
AU2002367294A1 (en) 2003-07-24
WO2003057836A2 (en) 2003-07-17
AU2008229989A1 (en) 2008-11-06
CA2471827A1 (en) 2003-07-17

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