CN204918618U - Suspended cell culture bottle - Google Patents
Suspended cell culture bottle Download PDFInfo
- Publication number
- CN204918618U CN204918618U CN201520563424.9U CN201520563424U CN204918618U CN 204918618 U CN204918618 U CN 204918618U CN 201520563424 U CN201520563424 U CN 201520563424U CN 204918618 U CN204918618 U CN 204918618U
- Authority
- CN
- China
- Prior art keywords
- bottle
- cell culture
- bottleneck
- arranges
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model provides a suspended cell culture bottle. Suspended cell culture bottle includes bottle, bottleneck and bottle lid, the upper surface of bottle sets up the culture medium and supplyes pipe and the additional tube cap of culture medium, the upper surface of bottle sets up sampling test pipe and sampling test tube cap, the bottleneck sets up in the bottle side to by upwards buckling to the bottle neck direction at the bottom of the bottleneck. The utility model discloses suspended cell culture bottle has easy and simple to handlely, and is manageable, deposit to the blake bottle advances appearance, the different kong yi of sample, avoids leading to the fact advantages such as pollution.
Description
Technical field
The utility model relates to biological technical field, in particular to a kind of suspending cell culture bottle.
Background technology
Cell culture technology is also cell clone technology, and the regular noun in biology is cell culture technology.No matter for whole biotechnology, or the biological cloning technology of one of them, cell cultures is all a requisite process, the extensive clone of cell cultures inherently cell.Cell culture technology can be become the many cells of simply unicellular or few differentiation through mass propgation by a cell, this is the requisite link of clone technology, and the clone of cell cultures inherently cell.A large amount of cells or its meta-bolites is obtained by cell cultures.Because biological product is all get from cell, so can say that cell culture technology is most crucial in biotechnology, most basic technology.
Suspension culture is a kind of conventional cell culture technology, and be a kind of being subject in the liquid nutrient medium constantly stirring or shake, cultivating tissue culture system that is unicellular and small cell cluster, is a kind of training method of non-anchorage-dependent cell.Some anchorage-dependent cell is through adapting to and selecting also to cultivate by this method.Increase suspension culture scale relatively simple, as long as it is just passable to increase volume.The degree of depth, more than 5mm, needs to stir substratum, more than 10cm, also needs deep layer to pass into CO
2and air, to ensure enough gaseous interchange.Cell is made to be in the cultural method of dispersion suspension in nutrient solution all the time by vibration or wheelwork.
The method utilizing the in vitro tissue of solid agar medium to plant to cultivate is widely used in plant genetic experiment.But also there are some shortcomings in some aspects in this method, such as in culturing process, the nutritive ingredient of callus in process of growth of plant, the metabolic substd that plant tissue produces presents a Gradient distribution, and agar itself also has some not clear material compositions may have an impact to culture, thus cause the change of metabolism in plant tissue growth growth course to utilize liquid nutrient medium then can overcome this shortcoming, when plant be organized in grow in liquid nutrient medium time, can be cultivated by thin layer concussion or in substratum, ventilate to improve the supply of oxygen in substratum.The suspension culture of vegetable cell refers to vegetable cell or less cell mass to be suspended in liquid nutrient medium and cultivates, and can keep good dispersion state in culturing process.These little Cell-aggregates are usually from the callus of plant.
General operating process undifferentiated callus is transferred in liquid nutrient medium cultivate.In culturing process, constantly carry out rotation concussion, generally the speed of available 100 ~ 120r/min is carried out.Due to rotation and the concussion of liquid nutrient medium, the cell that callus divides is made constantly to dissociate to get off.Culture in liquid medium within mixes, and existing free individual cells, also has larger cell mass, also has the dead cell residue of inoculum.
In China's Med Biol research, often use culturing bottle as the container of suspension cell culture.When enlarged culturing, need to change multiple Tissue Culture Flask, multiple Tissue Culture Flask is not easy management.And present Tissue Culture Flask sample introduction, sampling use same hole, easily pollute.
In view of this, special proposition the utility model.
Utility model content
The purpose of this utility model is to provide a kind of suspending cell culture bottle, and described suspending cell culture bottle has easy and simple to handle, manageable, deposit, and culturing bottle sample introduction, samples different Kong Yi, avoids advantages such as polluting.
In order to realize above-mentioned purpose of the present utility model, spy by the following technical solutions:
A kind of suspending cell culture bottle, comprises bottle, bottleneck and bottle cap; Described bottleneck is connected with bottle cap; The upper surface of described bottle arranges culture medium supplemented pipe and culture medium supplemented pipe lid; The upper surface of described bottle arranges sampling detector tube and sampling detector tube lid; Described bottleneck is arranged on the side of bottle, and by upwards bending to bottle neck direction at the bottom of bottleneck (equal with bottle at the bottom of bottleneck during normal use, bottle neck is higher than at the bottom of bottleneck).
Described bottleneck is connected preferably by screw thread with bottle cap; In described bottle cap, preferably seal washer is set, bottleneck can be sealed with bottle cap and be connected.
Described culture medium supplemented pipe and culture medium supplemented pipe lid are connected preferably by screw thread; In described culture medium supplemented pipe lid, preferably seal washer is set, makes culture medium supplemented pipe and culture medium supplemented pipe lid can seal connected.
Described sampling detector tube is connected preferably by screw thread with sampling detector tube lid; In described sampling detector tube lid, preferably seal washer is set, sampling detector tube can be sealed with sampling detector tube lid and be connected.
Described bottle neck preferably arranges filtering layer, and described filtering layer can filtrated air, described bottle cap cell inject and cell harvesting time open.
Described bottle is preferably integrated formed structure, and the part wherein near bottleneck is preferably ladder structure, and described bottle is preferably rectangular configuration away from the part of bottleneck.
The angle of the upper surface of described bottleneck and bottle is 120 ° to 135 °.
The diameter of described bottle neck is preferably 2cm, and the diameter of the described culture medium supplemented pipe mouth of pipe and the sampling detector tube mouth of pipe is preferably 1cm respectively.
The upper surface of described bottle preferably arranges panel, the shape of described panel is consistent with the upper surface shape of bottle, described panel arranges through hole at culture medium supplemented pipe and sampling detector tube, be respectively used to, through culture medium supplemented pipe and sampling detector tube, panel fully be contacted with the upper surface of bottle.
The upper surface of described panel and bottle is preferably by ultrasonic welding mode seal welding.
One side of described bottle preferably arranges draw-in groove, the another side relative with draw-in groove preferably arranges corresponding connector assembly, described grafting can be inserted in the draw-in groove of adjacent suspending cell culture bottle, makes adjacent suspending cell culture bottle stationary arrangement.
Or a side of described bottle preferably arranges buckle, the another side relative with buckle preferably arranges corresponding bayonet connection, described buckle can with the bayonet connection clamping of adjacent suspending cell culture bottle, make adjacent suspending cell culture bottle stationary arrangement.
The bottom surface of described bottle arranges culture medium supplemented pipe groove in the position corresponding with culture medium supplemented pipe; The internal diameter of described culture medium supplemented pipe groove is preferably not less than the external diameter of described culture medium supplemented pipe lid;
The bottom surface of described bottle arranges groove in the position corresponding with sampling detector tube and samples detector tube; The internal diameter of described sampling detector tube groove is preferably not less than the external diameter of described sampling detector tube lid.
The culture medium supplemented pipe being stamped culture medium supplemented pipe lid can insert culture medium supplemented pipe groove, the sampling detector tube being stamped sampling detector tube lid can insert sampling detector tube, multiple suspending cell culture bottle can mounted on top, and the bottom surface of suspending cell culture bottle preferably can contact with the panel of the suspending cell culture bottle of below.
Or described panel arranges draw-in groove near bottleneck bottom, bottom bottle bottom surface, correspondence position arranges edge, and draw-in groove and edge can be fixedly linked, and the mode that multiple suspending cell culture bottle can stack by putting the cart before the horse connects and fixes.
The surrounding of described panel preferably arranges flange, and bottle bottom surface surrounding correspondence position preferably arranges groove, and when multiple suspending cell culture bottle stacks, described flange can insert in the groove of adjacent suspending cell culture bottle bottle bottom surface and be fixed.
Described flange and/or groove preferably arrange breach, is convenient to separation when multiple suspending cell culture bottle stacks.
Or the surrounding of described panel preferably arranges bayonet connection, bottle bottom surface surrounding correspondence position preferably arranges buckle, and when multiple suspending cell culture bottle stacks, described bayonet connection can connect and fix with buckle.
Described bottle side preferably arranges scale marks.
Described panel and bottle medial surface are through surface hydrophilicity process.
Described bottle side preferably arranges abrasive block, is convenient to mark.
Compared with prior art, the beneficial effects of the utility model are:
(1) multiple suspending cell culture bottle can stack and deposit and move, and saves incubator space, convenient management.
(2) described bottle side arranges abrasive block, be convenient to mark.
(3) described bottleneck is arranged on bottle side, and upwards bends to bottle neck direction by the bottom of bottleneck, and like this when cell cultures keeps flat, bottle neck position is higher, uses during more substratum and can not overflow, and conveniently from culturing bottle, extracts cell.
(4) angle of the upper surface of described bottleneck and bottle is 120 ° to 135 °, like this, when Tissue Culture Flask keeps flat, transfer pipet or cell scraper can touch all positions of cultivating bottom surface, facilitate harvested cell, avoid culturing bottle to produce the extraction dead angle of not easily extracting cell.
(5) suspending cell culture bottle described in has easy and simple to handle, manageable, deposit, and culturing bottle sample introduction, samples different Kong Yi, avoids advantages such as polluting.
Accompanying drawing explanation
In order to be illustrated more clearly in the utility model embodiment or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the utility model suspending cell culture bottle side-looking structural representation;
Fig. 2 is the utility model suspending cell culture bottle plan structure schematic diagram;
Fig. 3 is the utility model suspending cell culture bottle forward sight structural representation;
Reference numeral:
1-bottle cap; 2-bottleneck; 3-panel;
4-bottle; 5-culture medium supplemented pipe lid; 6-culture medium supplemented pipe;
7-samples detector tube lid; 8-samples detector tube; 9-abrasive block;
10-filtering layer.
Embodiment
Below in conjunction with the drawings and specific embodiments, the technical solution of the utility model is clearly and completely described, but it will be understood to those of skill in the art that, following described embodiment is the utility model part embodiment, instead of whole embodiments, only for illustration of the utility model, and should not be considered as limiting scope of the present utility model.Based on the embodiment in the utility model, those of ordinary skill in the art are not making the every other embodiment obtained under creative work prerequisite, all belong to the scope of the utility model protection.Unreceipted actual conditions person in embodiment, the condition of conveniently conditioned disjunction manufacturers suggestion is carried out.Agents useful for same or the unreceipted production firm person of instrument, be and can buy by commercially available the conventional products obtained.
In description of the present utility model, it should be noted that, orientation or the position relationship of the instruction such as term " " center ", " on ", D score, "left", "right", " vertically ", " level ", " interior ", " outward " they be based on orientation shown in the drawings or position relationship; be only the utility model and simplified characterization for convenience of description; instead of instruction or imply the device of indication or element must have specific orientation, with specific azimuth configuration and operation, therefore can not be interpreted as restriction of the present utility model.In addition, term " first ", " second ", " the 3rd " only for describing object, and can not be interpreted as instruction or hint relative importance.
In description of the present utility model, it should be noted that, unless otherwise clearly defined and limited, term " installation ", " being connected ", " connection " should be interpreted broadly, and such as, can be fixedly connected with, also can be removably connect, or connect integratedly; Can be mechanical connection, also can be electrical connection; Can be directly be connected, also indirectly can be connected by intermediary, can be the connection of two element internals.For the ordinary skill in the art, particular case the concrete meaning of above-mentioned term in the utility model can be understood.
As shown in Figure 1-Figure 3, the utility model provides a kind of suspending cell culture bottle, comprises bottle 4, bottleneck 2 and bottle cap 1; The upper surface of described bottle 4 arranges culture medium supplemented pipe 6 and culture medium supplemented pipe lid 5; The upper surface of described bottle 4 arranges sampling detector tube 8 and sampling detector tube lid 7; Described bottleneck 2 is arranged on bottle 4 side, and is upwards bent to bottleneck 2 mouthfuls of directions by bottleneck 2 end.
Described bottleneck 2 is connected preferably by screw thread with bottle cap 1; In described bottle cap 1, preferably seal washer is set, bottleneck 2 can be sealed with bottle cap 1 and be connected.
Described culture medium supplemented pipe 6 and culture medium supplemented pipe lid 5 are connected preferably by screw thread; In described culture medium supplemented pipe lid 5, preferably seal washer is set, makes culture medium supplemented pipe 6 and culture medium supplemented pipe lid 5 can seal connected.
Described sampling detector tube 8 is connected preferably by screw thread with sampling detector tube lid 7; In described sampling detector tube lid 7, preferably seal washer is set, sampling detector tube 8 can be sealed with sampling detector tube lid 7 and be connected.
Described bottleneck 2 mouthfuls preferably arranges filtering layer 10, and described filtering layer 10 can filtrated air, described bottle cap 1 cell inject and cell harvesting time open.
Described bottle 4 is preferably integrated formed structure, and the part wherein near bottleneck 2 is preferably ladder structure, and described bottle 4 is preferably rectangular configuration away from the part of bottleneck 2.
Described bottleneck 2 is 120 ° to 135 ° with the angle of the upper surface of bottle 4.
The upper surface of described bottle 4 preferably arranges panel 3, the shape of described panel 3 is consistent with the upper surface shape of bottle 4, described panel 3 arranges through hole at culture medium supplemented pipe 6 and sampling detector tube 8, be respectively used to, through culture medium supplemented pipe 6 and sampling detector tube 8, panel 3 fully be contacted with the upper surface of bottle 4.
The upper surface of described panel 3 and bottle 4 is preferably by ultrasonic welding mode seal welding.
Described bottle 4 one side preferably arranges draw-in groove, and the another side relative with draw-in groove preferably arranges corresponding connector assembly, and described grafting can be inserted in the draw-in groove of adjacent suspending cell culture bottle, makes adjacent suspending cell culture bottle stationary arrangement.
Or a side of described bottle 4 preferably arranges buckle, the another side relative with buckle preferably arranges corresponding bayonet connection, described buckle can with the bayonet connection clamping of adjacent suspending cell culture bottle, make adjacent suspending cell culture bottle stationary arrangement.
The bottom surface of described bottle 4 arranges culture medium supplemented pipe groove in the position corresponding with culture medium supplemented pipe 6; The internal diameter of described culture medium supplemented pipe groove is preferably not less than the external diameter of described culture medium supplemented pipe lid 5;
The bottom surface of described bottle 4 arranges groove in the position corresponding with sampling detector tube 8 and samples detector tube 8; The internal diameter of described sampling detector tube groove is preferably not less than the external diameter of described sampling detector tube lid 7.
The culture medium supplemented pipe 6 being stamped culture medium supplemented pipe lid 5 can insert culture medium supplemented pipe groove, the sampling detector tube 8 being stamped sampling detector tube lid 7 can insert sampling detector tube 8, multiple suspending cell culture bottle can mounted on top, and the bottom surface of suspending cell culture bottle preferably can contact with the panel 3 of the suspending cell culture bottle of below.
Or described panel 3 arranges draw-in groove near bottleneck 2 bottom, bottom bottle 4 bottom surface, correspondence position arranges edge, and draw-in groove and edge can be fixedly linked, and the mode that multiple suspending cell culture bottle can stack by putting the cart before the horse connects and fixes.
The surrounding of described panel 3 preferably arranges flange, and bottle 4 bottom surface surrounding correspondence position preferably arranges groove, and when multiple suspending cell culture bottle stacks, described flange can insert in the groove of adjacent suspending cell culture bottle bottle 4 bottom surface and be fixed.
Described flange and/or groove preferably arrange breach, is convenient to separation when multiple suspending cell culture bottle stacks.
Or the surrounding of described panel 3 preferably arranges bayonet connection, bottle 4 bottom surface surrounding correspondence position preferably arranges buckle, and when multiple suspending cell culture bottle stacks, described bayonet connection can connect and fix with buckle.
Described bottle 4 side preferably arranges scale marks.
Described panel 3 and bottle 4 medial surface are through surface hydrophilicity process.
Described bottle 4 side preferably arranges abrasive block 9, is convenient to mark.
Compared with prior art, the beneficial effects of the utility model are:
(1) multiple suspending cell culture bottle can stack and deposit and move, and saves incubator space, convenient management.
(2) described bottle 4 side arranges abrasive block 9, be convenient to mark.
(3) described bottleneck 2 is arranged on bottle 4 side, and is upwards bent to bottleneck 2 mouthfuls of directions by bottleneck 2 end, and like this when cell cultures keeps flat, bottleneck 2 mouthfuls of positions are higher, uses during more substratum and can not overflow, and conveniently from culturing bottle, extracts cell.
(4) described bottleneck 2 is 120 ° to 135 ° with the angle of the upper surface of bottle 4, like this, when Tissue Culture Flask keeps flat, transfer pipet or cell scraper can touch all positions of cultivating bottom surface, facilitate harvested cell, avoid culturing bottle to produce the extraction dead angle of not easily extracting cell.
(5) suspending cell culture bottle described in has easy and simple to handle, manageable, deposit, and culturing bottle sample introduction, samples different Kong Yi, avoids advantages such as polluting.
Although illustrate and describe the utility model with specific embodiment, however it will be appreciated that can to make when not deviating from spirit and scope of the present utility model many other change and amendment.Therefore, this means to comprise all such changes and modifications belonged within the scope of the utility model in the following claims.
Claims (10)
1. a suspending cell culture bottle, is characterized in that: comprise bottle, bottleneck and bottle cap; Described bottle cap is connected with bottleneck; The upper surface of described bottle arranges culture medium supplemented pipe and culture medium supplemented pipe lid; The upper surface of described bottle arranges sampling detector tube and sampling detector tube lid; Described bottleneck is arranged on the side of bottle, and upwards bends to bottle neck direction by the bottom of bottleneck.
2. a kind of suspending cell culture bottle according to claim 1, is characterized in that: described bottleneck is connected by screw thread with bottle cap.
3. a kind of suspending cell culture bottle according to claim 1, is characterized in that: described culture medium supplemented pipe and culture medium supplemented pipe lid are connected by screw thread.
4. a kind of suspending cell culture bottle according to claim 1, is characterized in that: described sampling detector tube is connected by screw thread with sampling detector tube lid.
5. a kind of suspending cell culture bottle according to claim 1, is characterized in that: described bottle neck arranges filtering layer.
6. a kind of suspending cell culture bottle according to claim 1, is characterized in that: the angle of the upper surface of described bottleneck and bottle is 120 ° to 135 °.
7. a kind of suspending cell culture bottle according to claim 1, is characterized in that: the upper surface of described bottle arranges panel.
8. a kind of suspending cell culture bottle according to claim 1, is characterized in that: described bottle side arranges scale marks.
9. a kind of suspending cell culture bottle according to claim 1, is characterized in that: the side of described bottle arranges scale marks.
10. a kind of suspending cell culture bottle according to claim 1, is characterized in that: the side of described bottle arranges abrasive block.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520563424.9U CN204918618U (en) | 2015-07-30 | 2015-07-30 | Suspended cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520563424.9U CN204918618U (en) | 2015-07-30 | 2015-07-30 | Suspended cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204918618U true CN204918618U (en) | 2015-12-30 |
Family
ID=54967764
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520563424.9U Active CN204918618U (en) | 2015-07-30 | 2015-07-30 | Suspended cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204918618U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471592A (en) * | 2020-05-11 | 2020-07-31 | 华中科技大学同济医学院附属协和医院 | Cell culture bottle and manufacturing method thereof |
-
2015
- 2015-07-30 CN CN201520563424.9U patent/CN204918618U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111471592A (en) * | 2020-05-11 | 2020-07-31 | 华中科技大学同济医学院附属协和医院 | Cell culture bottle and manufacturing method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN206872853U (en) | Biological cell culture apparatus | |
| CN203513696U (en) | Cell culture flask | |
| CA3086081A1 (en) | Bioreactors for root organ cultures | |
| CN204644371U (en) | A kind of integrated form many cells noncontact co-culture device | |
| CN204918618U (en) | Suspended cell culture bottle | |
| CN202190561U (en) | Cordyceps militaris culturing box | |
| CN202369595U (en) | Cell culture flask | |
| CN111925936A (en) | Urine stem cell preparation equipment | |
| CN106701659A (en) | Porcine ovarian granulosa cell primary culture method | |
| CN217025936U (en) | Cell culture bottle | |
| CN108504574A (en) | Culture bottle | |
| CN210856118U (en) | Molecular biology culture dish | |
| CN204981891U (en) | Cell culture changes bottle | |
| CN104025904A (en) | Culturing method for tricholoma matsutake mycelia and special device thereof | |
| CN205258488U (en) | Novel embryo culture ware | |
| CN201501859U (en) | Fungal microculture device capable of being directly observed under microscope | |
| CN204417516U (en) | The special Micro-CPE neutralization test ware of laser scanning co-focusing microscope | |
| CN207047253U (en) | A kind of culture device of dermis fibroblast | |
| CN208151372U (en) | A kind of new indoor experiment microdisk electrode column photosynthesis physiological target | |
| CN203429183U (en) | Three-dimensional cell culture plate | |
| CN205741065U (en) | A kind of Tissue Culture Dish | |
| CN213803842U (en) | Cell culture device for biotechnology | |
| CN206359538U (en) | A kind of Suspension Culture Flask | |
| WO2018086326A1 (en) | Photobioreactor | |
| CN209338568U (en) | A kind of living cells culture apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: No. 316, floor 3, building 1, block B, No. 80, xingshikou Road, Haidian District, Beijing 100093 Patentee after: Beijing Yimiao Shenzhou Pharmaceutical Technology Co., Ltd Address before: 100000 , Tsinghua Park, Tsinghua University, research and research building, eight floor, B801-089, Beijing Patentee before: BEIJING IMMUNO SHENZHOU MEDICAL TECHNOLOGY Co.,Ltd. |
|
| CP03 | Change of name, title or address | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: Shanghai xianbo Biotechnology Co., Ltd Assignor: Beijing Yimiao Shenzhou Pharmaceutical Technology Co.,Ltd. Contract record no.: X2020320000048 Denomination of utility model: Suspended cell culture bottle Granted publication date: 20151230 License type: Exclusive License Record date: 20200722 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Shanghai xianbo Biotechnology Co., Ltd Assignor: Beijing Yimiao Shenzhou Pharmaceutical Technology Co.,Ltd. Contract record no.: X2020320000048 Date of cancellation: 20211218 |
|
| EC01 | Cancellation of recordation of patent licensing contract |