CN204981891U - Cell culture changes bottle - Google Patents

Abstract

A cell culture spinner bottle, comprising a bottle body with an opening, a bottleneck fixedly connected to the opening end of the bottle body, and a bottle cap, the bottle cap and the bottle neck are fixedly connected in a threaded manner, the bottle body includes a bottom wall and a The side wall of the surface periphery, the curve formed by the longitudinal section of the bottle intercepting the inner surface of the side wall is a wavy line. In the cell culture spinner bottle of the present utility model, the curve formed by the longitudinal section of the bottle body intercepting the inner surface of the side wall is a wavy line, so that the inner surface of the bottle body can be increased without changing the size of the bottle body. The area makes the cultured cells loose, easy to collect, and simple in structure.

Description

A spinner bottle for cell culture

technical field

The utility model relates to the technical field of cell culture equipment, in particular to a cell culture spinner bottle.

Background technique

Some cells and tissues can grow in suspension, but a considerable number of mammalian cells need to be attached to in vitro culture scaffolds for adherent growth. Therefore, in addition to good transparency, non-toxic and sterile, the culture flasks, culture plates and culture dishes used to provide the environment for in vitro culture of cells also need surface modification to make the surface hydrophilic and make the adherent type grow cells are able to attach, divide and grow. In the existing cell culture bottle, it mainly includes a bottle body, a bottleneck and a bottle cap, and the bottom of the bottle body is subjected to hydrophilic treatment so that the cells adhere to the bottom and grow, and the area of the bottom is relatively small compared to the area of the whole bottle body, and the growth of the cultured cells is relatively small. Space is limited.

Utility model content

Aiming at the deficiencies of the prior art, the purpose of this utility model is to provide a cell culture spinner bottle, which increases the area of the inner surface of the bottle for cell attachment without changing the size of the bottle body.

In order to achieve the above object, the utility model adopts the following technical solutions:

A cell culture spinner bottle, comprising a bottle body with an opening, a bottleneck fixedly connected to the opening end of the bottle body, and a bottle cap, the bottle cap and the bottle neck are fixedly connected in a threaded manner, the bottle body includes a bottom wall and a The side wall of the surface periphery, the curve formed by the longitudinal section of the bottle intercepting the inner surface of the side wall is a wavy line.

Preferably, the curve formed by the longitudinal section of the bottle body intercepting the outer surface of the side wall is a wavy line.

Preferably, the outer surface of the side wall is provided with scale marks.

Preferably, the upper surface of the bottom wall protrudes upwards to form a boss.

Preferably, the bottle body is cylindrical or cylindrical.

The beneficial effects of the utility model are:

Compared with the prior art, in the cell culture spinner bottle of the present utility model, the curve formed by the longitudinal section of the bottle body intercepting the inner surface of the side wall is a wavy line, so that the inner surface of the bottle body can be enlarged without changing the size of the bottle body The area available for cell attachment makes the cultured cells loose, easy to collect, and simple in structure.

Description of drawings

Fig. 1 is the structural representation of the utility model;

Fig. 2 is the A direction view in Fig. 1;

Fig. 3 is a schematic cross-sectional view of the longitudinal section of the bottle body of the present invention intercepting the inner surface of the side wall.

Wherein, 10, bottle body; 11, bottom wall; 12, side wall; 13, wavy line; 14, scale line; 15, boss; 20, bottleneck; 30, bottle cap.

detailed description

Below, in conjunction with accompanying drawing and specific embodiment, the utility model is described further:

A cell culture spinner bottle as shown in Fig. 1, 2 and Fig. 3 includes a bottle body 10, a bottleneck 20 and a bottle cap 30, and the bottle body 10 has an opening at one end, which is convenient for putting in the cell culture solution. The bottle neck 20 is fixedly connected to the open end of the bottle body 10 , and the bottle cap 30 is screwed to the bottle neck 20 to seal the opening of the bottle body 10 , which helps the growth of cells in the bottle body 10 .

The above-mentioned bottle body 10 includes a bottom wall 11 and a side wall 12. The curve formed by the longitudinal section of the bottle body 10 intercepting the inner surface of the side wall 12 is a wavy line 13. In this way, on the premise of changing the size of the bottle body 10, the number of bottles is increased. The area of the inner surface of the body 10 that can be used for cell attachment makes the distribution of cultured cells in the bottle body 10 relatively loose, which is convenient for experimental collection; at the same time, the setting of this structure can better make the cells in the bottle body 10 adhere to the wall. The growth state is good.

Preferably, in this embodiment, the curve formed by the longitudinal section of the above-mentioned bottle body 10 intercepting the outer surface of the side wall 12 may also be a wavy line 13, so as to prevent the possible slipping of the bottle body 10 when the user holds it in hand, so as to facilitate handling. put.

Preferably, a scale line 14 can be provided on the outer surface of the side wall 12, and the position of the line of the scale line 14 can be consistent with that of the wavy line 13, which is convenient for recording the cell volume in the bottle body 10 and observing the cell growth rate. Preferably, the upper surface of the above-mentioned bottom wall 11 can be provided with a boss 15, which can be formed by protruding upward from the upper surface of the bottom wall 11, so that the cell growth space inside the bottle body 10 can be further increased to facilitate sampling .

Preferably, in this embodiment, the above-mentioned bottle body 10 can be specifically a cylindrical or cylindrical structure, and its cross-section is used as a hydrophilic treatment surface, which is much larger than that of a common bottle bottom. framework, which further amplifies the adherent growth surface.

In the cell culture spinner bottle of the present utility model, the curve formed by the longitudinal section of the bottle body 10 intercepting the inner surface of the side wall 12 is a wave line 13, so that the inner surface of the bottle body 10 can be increased without changing the size of the bottle body 10. The area for the cell to adhere to the wall makes the cultured cells relatively loose, easy to collect, and simple in structure.

For those skilled in the art, various other corresponding changes and deformations can be made according to the technical solutions and ideas described above, and all these changes and deformations should fall within the protection scope of the claims of the present utility model.

Claims (5)

1. a cell-culturing rotating bottle, comprise the bottle with opening, the bottleneck be fixedly connected with the opening end of bottle and bottle cap, bottle cap is fixedly connected with in the mode of thread fit with bottleneck, it is characterized in that, bottle comprises diapire and is positioned at the sidewall of bottom wall upper surface periphery, and formed by the internal surface of the longitudinal cross-section intercepting sidewall of bottle, curve is wavy line.

2. cell-culturing rotating bottle as claimed in claim 1, is characterized in that, formed by the outside surface of the longitudinal cross-section intercepting sidewall of bottle, curve is wavy line.

3. cell-culturing rotating bottle as claimed in claim 1, it is characterized in that, the outside surface of sidewall is provided with scale marks.

4. cell-culturing rotating bottle as claimed in claim 1, is characterized in that, the upper surface formation boss protruding upward of diapire.

5. cell-culturing rotating bottle as claimed in claim 1, it is characterized in that, bottle is cylindricality or tubular.