CN203238261U - Cell culture dish specially used for cell climbing piece - Google Patents
Cell culture dish specially used for cell climbing piece Download PDFInfo
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- CN203238261U CN203238261U CN 201320287220 CN201320287220U CN203238261U CN 203238261 U CN203238261 U CN 203238261U CN 201320287220 CN201320287220 CN 201320287220 CN 201320287220 U CN201320287220 U CN 201320287220U CN 203238261 U CN203238261 U CN 203238261U
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- culture dish
- cell
- cell culture
- slide glass
- shallow slot
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Abstract
The utility model discloses a cell culture dish specially used for a cell climbing piece. The cell culture dish comprises a cell culture dish body and a culture dish cover thereof, wherein at least one shallow slot matched with the shape of a glass slide is formed in the bottom surface of the cell culture dish body. When the cell culture dish specially used for the cell climbing piece is used, the glass slide is put into each shallow slot in the bottom surface of the cell culture dish body, so that the glass slide does not have a height difference in the cell culture dish, and cells can easily climb on the platform and grow up; therefore, the cell climbing effect is good; moreover, the glass slide in each shallow slot is easy to take out, and experiment operation is facilitated; the subsequent experiment operation procedure can be simplified.
Description
Technical field
The utility model relates to a kind of use for laboratory cell climbing sheet special cell culture dish.
Background technology
Cell climbing sheet is the important technical of Cell Biology Experiment, is usually used in the experiment that some combining forms are learned position observation intracellular protein or dna level, such as immunohistochemistry, immunofluorescence and fluorescence in situ hybridization etc.
Manufacture at present cell climbing sheet and still do not have special equipment, the method that common laboratory is manufactured cell climbing sheet is as follows: use cover glass to be carrier, put into ordinary cells culture dish bottom, adding cell suspension cultivates again, cell has the habit of adherent growth, cover glass can be taken out after cell firmly is attached on the cover glass.But because cover glass is very thin, thickness only about 0.15mm, easily is adsorbed on the culture dish bottom, difficult during taking-up, and cover glass is frangible, therefore when culture dish takes out and follow-up test very easily damage cover glass operating, caused very large difficulty to experimental implementation, experimental result also is difficult for preserving.In addition, the conventional slide glass that adopts of follow-up test (such as immunohistochemistry, immunofluorescence and fluorescence in situ hybridization) is as carrier, and the instruments such as the automatic staining instrument that a lot of units are equipped with, active immunity group instrument, automatic mounting machine are carrier design for slide glass all, so need the cover glass behind the creep plate is bundled or sticks on the slide glass, complex operation is not easy to the observation experiment result.If directly use slide glass to carry out cell climbing sheet as carrier, although slide glass is solid non-friable, easy to operate, easily preserve, but slide glass is thicker, puts into the ordinary cells culture dish and has height fall, cell can not be climbed up this platform growth, so the cell climbing sheet effect is often very poor.Up to this point also there is not a kind of utensil that is exclusively used in the making in laboratory cell climbing sheet.
The utility model content
The purpose of this utility model is to provide a kind of cell climbing sheet special cell culture dish.
The purpose of this utility model can be achieved through the following technical solutions:
A kind of cell climbing sheet special cell culture dish, it includes Tissue Culture Dish body and culture dish thereof lid, is provided with the shallow slot that at least one matches with the slide glass shape in the lower surface of this Tissue Culture Dish body.Described cooperation refers to that the size of shallow slot can guarantee that slide glass does not rock in shallow slot, can guarantee again to leave the gap between slide glass and the shallow slot inwall, is convenient to take out slide glass with instrument such as tweezers.
The quantity of described shallow slot is two or more.
Quantity is that two or more shallow slots are set up in parallel in Tissue Culture Dish body lower surface.
Above-mentioned shallow slot is arranged on the mid-way of Tissue Culture Dish body lower surface.
One end of above-mentioned shallow slot is integrally formed with the deep-slotted chip breaker of being convenient to take out with hand or instrument slide glass.
Above-mentioned slide glass is conventional slide glass, and the bottom surface of described Tissue Culture Dish body is the thickening bottom surface, and the thickness of its bottom surface is convenient to arrange the shallow slot that matches with the slide glass shape greater than the thickness of slide glass.
The slide glass that uses in the Tissue Culture Dish of the utility model design is commercially available conventional slide glass (size 25.4 * 76.2mm, thick 1.2 ~ 1.7mm), when slide glass is put into shallow slot, the upper level of this slide glass is identical with Tissue Culture Dish body bottom surface height around it, makes cell can evenly be grown in Tissue Culture Dish body bottom and is attached on the slide glass.The deep-slotted chip breaker that is located at shallow slot one end conveniently takes out slide glass.
The beneficial effects of the utility model:
The cell climbing sheet special cell culture dish of the utility model design, during use, slide glass is put into the shallow slot that is located at Tissue Culture Dish body lower surface, make slide glass in Tissue Culture Dish, not have height fall, cell can be easy to climb up this platform growth, so cell climbing sheet is effective.And the slide glass that is located in the shallow slot takes out easily, is convenient to experimental implementation.Owing to directly substituted cover glass as carrier with slide glass, simplified experimental procedure, overcome the defective of frangible, the difficult preservation of cover glass, operational difficulty, and can use self-reacting device to carry out follow-up test, saved manpower, optimized the follow-up test effect.
Description of drawings
Fig. 1 is the vertical view of cell climbing sheet special cell culture dish body.
Fig. 2 is the sectional view that is provided with the cell climbing sheet special cell culture dish body A-A direction of slide glass in the shallow slot.
Fig. 3 is the sectional view of not putting the cell climbing sheet special cell culture dish body A-A direction of slide glass in the shallow slot.
Fig. 4 is the sectional view of cell climbing sheet special cell culture dish body B-B direction.
Embodiment
Such as Fig. 1, Fig. 2 and shown in Figure 3, a kind of cell climbing sheet special cell culture dish, it includes Tissue Culture Dish body 1 and culture dish thereof lid, is provided with side by side two shallow slots 3 that match with slide glass 2 shapes in the mid-way of the lower surface of this Tissue Culture Dish body 1.An end of described shallow slot 3 is integrally formed with the deep-slotted chip breaker 4 of being convenient to hand or instrument such as tweezers taking-up slide glass 2 as shown in Figure 4.Described slide glass is commercially available conventional slide glass.The bottom surface of described Tissue Culture Dish body 1 is the thickening bottom surface, and the thickness of its bottom surface is convenient to offer the shallow slot 3 that matches with the slide glass shape greater than the thickness of slide glass 2.
During use, slide glass 2 is put into the shallow slot 3 that is located at Tissue Culture Dish body 1 lower surface, make slide glass 2 in Tissue Culture Dish body 1, not have height fall, then in Tissue Culture Dish body 1, add cell suspension, the culture dish lid also is a plakea, its internal diameter is slightly larger than Tissue Culture Dish body 1 external diameter, and it is tipped upside down on the Tissue Culture Dish body 1.During cultivation, cell attachment is grown in slide glass 2 upper surfaces, so cell climbing sheet is effective.And the deep-slotted chip breaker 4 that one is located at shallow slot 3 one ends makes slide glass be convenient to take out with hand or instrument, and is easy to operate.Because the utility model directly uses slide glass to manufacture cell climbing sheet as carrier, simplified experimental procedure, overcome the defective that cover glass is frangible, be difficult for preservation, operational difficulty, the cell climbing sheet made from slide glass 2 can directly use at self-reacting device (automatic staining instrument, active immunity group instrument, automatically mounting machine etc.) and carry out follow-up test (such as immunohistochemistry, immunofluorescence and fluorescence in situ hybridization etc.), save manpower, optimized the effect of follow-up test.
Claims (7)
1. cell climbing sheet special cell culture dish, it includes Tissue Culture Dish body (1) and culture dish lid thereof, it is characterized in that being provided with the shallow slot (3) that at least one matches with slide glass (2) shape in the lower surface of this Tissue Culture Dish body (1).
2. cell climbing sheet special cell culture dish according to claim 1 is characterized in that the quantity of described shallow slot (3) is two or more.
3. cell climbing sheet special cell culture dish according to claim 2 is characterized in that described shallow slot (3) is set up in parallel in Tissue Culture Dish body (1) lower surface.
4. the described cell climbing sheet special cell culture dish of any one according to claim 1-3 is characterized in that described shallow slot (3) is arranged on the mid-way of Tissue Culture Dish body (1) lower surface.
5. the described cell climbing sheet special cell culture dish of any one according to claim 1-3 is characterized in that an end of described shallow slot (3) is integrally formed with the deep-slotted chip breaker (4) of being convenient to take out with hand or instrument slide glass (2).
6. cell climbing sheet special cell culture dish according to claim 4 is characterized in that an end of described shallow slot (3) is integrally formed with the deep-slotted chip breaker (4) of being convenient to take out with hand or instrument slide glass (2).
7. cell climbing sheet special cell culture dish according to claim 1 is characterized in that described slide glass is conventional slide glass, and the bottom surface of described Tissue Culture Dish body (1) is the thickening bottom surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320287220 CN203238261U (en) | 2013-05-21 | 2013-05-21 | Cell culture dish specially used for cell climbing piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320287220 CN203238261U (en) | 2013-05-21 | 2013-05-21 | Cell culture dish specially used for cell climbing piece |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203238261U true CN203238261U (en) | 2013-10-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320287220 Expired - Fee Related CN203238261U (en) | 2013-05-21 | 2013-05-21 | Cell culture dish specially used for cell climbing piece |
Country Status (1)
| Country | Link |
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| CN (1) | CN203238261U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104359741A (en) * | 2014-09-26 | 2015-02-18 | 南通大学 | Application of multi-functional PAP pen in cell climbing immunohistochemical detection |
| CN112080429A (en) * | 2020-09-09 | 2020-12-15 | 江苏益玛生物科技有限公司 | High stability surface biological cell culture dish |
| CN112094745A (en) * | 2020-09-09 | 2020-12-18 | 江苏益玛生物科技有限公司 | Processing method of high-stability surface biological cell culture dish |
-
2013
- 2013-05-21 CN CN 201320287220 patent/CN203238261U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104359741A (en) * | 2014-09-26 | 2015-02-18 | 南通大学 | Application of multi-functional PAP pen in cell climbing immunohistochemical detection |
| CN104359741B (en) * | 2014-09-26 | 2017-01-11 | 南通大学 | Application of multi-functional PAP pen in cell climbing immunohistochemical detection |
| CN112080429A (en) * | 2020-09-09 | 2020-12-15 | 江苏益玛生物科技有限公司 | High stability surface biological cell culture dish |
| CN112094745A (en) * | 2020-09-09 | 2020-12-18 | 江苏益玛生物科技有限公司 | Processing method of high-stability surface biological cell culture dish |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131016 Termination date: 20140521 |