CN112080429A - High stability surface biological cell culture dish - Google Patents
High stability surface biological cell culture dish Download PDFInfo
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- CN112080429A CN112080429A CN202010938895.9A CN202010938895A CN112080429A CN 112080429 A CN112080429 A CN 112080429A CN 202010938895 A CN202010938895 A CN 202010938895A CN 112080429 A CN112080429 A CN 112080429A
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- dish
- cell culture
- biological cell
- culture dish
- high stability
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- 238000004113 cell culture Methods 0.000 title claims abstract description 26
- 230000009194 climbing Effects 0.000 claims abstract description 45
- 238000009832 plasma treatment Methods 0.000 claims abstract description 6
- 230000000994 depressogenic effect Effects 0.000 claims description 16
- 239000011521 glass Substances 0.000 claims description 8
- 229920008790 Amorphous Polyethylene terephthalate Polymers 0.000 claims description 6
- 239000003292 glue Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 239000000499 gel Substances 0.000 claims description 3
- 239000012943 hotmelt Substances 0.000 claims description 3
- 238000001746 injection moulding Methods 0.000 claims description 3
- 239000001993 wax Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 10
- 238000000926 separation method Methods 0.000 description 8
- 239000002775 capsule Substances 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 230000010261 cell growth Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 229920005372 Plexiglas® Polymers 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000005388 borosilicate glass Substances 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a high-stability surface biological cell culture dish, which comprises a dish body, a dish cover and a climbing sheet, wherein the dish body is provided with a first opening and a second opening; a polygonal sunken area is arranged in the middle of the bottom surface of the dish body, a through hole is arranged in the middle of the sunken area, the shape and the thickness of the climbing sheet are the same as those of the sunken area, the climbing sheet is fixed on the sunken area, and the upper surface of the climbing sheet is subjected to plasma treatment; the upper edge of the dish body and the lower edge of the dish cover are provided with staggered convex and concave structures which are uniformly distributed, and the shapes of the convex and concave structures are the same; the upper edge of the dish body is provided with a ditch, the lower edge of the dish cover is provided with a protruding part corresponding to the ditch, and the height and the width of the protruding part are consistent with the depth and the width of the ditch.
Description
Technical Field
The invention belongs to the field of medical article processing, and particularly relates to a culture dish convenient for cell culture.
Background
Cell culture is the most central and basic technology in biotechnology; a petri dish is a laboratory vessel used for microbial or cell culture. The biological cell culture dish commonly used at present is made of borosilicate glass, and a climbing sheet used for bearing microorganisms or cells is arranged in the middle area of the bottom of the culture dish. Attachment and extension of biological cells during culture is one of the important indicators, and although attachment and extension are the basic growth characteristics of most in vitro cultured cells, individual cells have poor attachment and extension characteristics during growth, such as lymphocytes. In order to improve the adhesion property and the stability of the biological cell culture dish, some special substances for promoting cell adhesion, such as laminin, fibronectin, type III18 collagen, serum expansion factor, etc., are currently used. In other words, the special cells cannot grow normally on the biological cell culture dish, especially the attachment and extension cannot be guaranteed, the stability is poor, and further the special cells cannot be subjected to related experiments.
Disclosure of Invention
In order to solve the problem that the attachment and extension of special cells on a culture dish are difficult to ensure, the invention designs a high-stability surface biological cell culture dish, which comprises: a dish body, a dish cover and a climbing sheet; a polygonal sunken area is arranged in the middle of the bottom surface of the dish body, a through hole is arranged in the middle of the sunken area, the shape and the thickness of the climbing sheet are the same as those of the sunken area, the climbing sheet is fixed on the sunken area, and the upper surface of the climbing sheet is subjected to plasma treatment; the upper edge of the dish body and the lower edge of the dish cover are provided with staggered convex and concave structures which are uniformly distributed, and the shapes of the convex and concave structures are the same; the upper edge of the dish body is provided with a ditch, the lower edge of the dish cover is provided with a protruding part corresponding to the ditch, and the height and the width of the protruding part are consistent with the depth and the width of the ditch.
Preferably, the horizontal projection of the protrusion is in a wave trough shape of a rectangle, a trapezoid or a sine wave.
Preferably, the climbing piece is made of APET materials, the upper portions of two sides of the concave area are provided with inward extending separation blades, the separation blades and the dish body are integrally formed in an injection molding mode, one side of the climbing piece is abutted to the lower end of the separation blade during installation, and the other side of the climbing piece is clamped into the separation blade on the other side of the climbing piece.
Preferably, the climbing piece is made of organic glass, the edge of the depressed area and the edge of the climbing piece are sealed by transparent glue or glass cement or wax or hot melt, the longitudinal section of the climbing piece is trapezoidal, one base angle is obliquely cut off, the included angle between one oblique edge and the base edge is 80-85 degrees, the included angle between the other oblique edge and the bottom is 100-135 degrees, the included angle between the other oblique edge and the upper edge is 100-135 degrees, the shape of the longitudinal section of the depressed area is identical to that of the longitudinal section of the climbing piece, and the dish body is integrally injection-molded.
Preferably, a rubber ring or a gel ring or a silica gel ring is fixed in the ditch.
Compared with a common cell culture dish, the invention has the following advantages:
1. the surface of the climbing sheet is subjected to plasma treatment, so that cell attachment and extension are facilitated, and the shape design matched with the concave area enables the climbing sheet to be mounted and dismounted conveniently and quickly, and the observation quality is good;
2. the culture dish has good air permeability and sealing performance, and the structural design of the combination part of the dish cover and the dish body ensures that the dish cover can be tightly closed on the dish body when not in use, and can also form a plurality of ventilating holes on the bulge part of the upper edge of the dish body for facilitating ventilation when the bulge of the dish cover is buckled on the bulge part of the upper edge of the dish body during cell culture, and the dish cover can not slide off from the transverse direction due to the design of a channel;
3. only APET or plexiglass is selected among the wide variety of resins because it may not contain 19 chemicals that affect cell growth, thereby improving the quality of cell growth in the biological cell culture dish. APET or organic glass has excellent optical performance and low dielectric constant. In summary, the optical properties of the material, such as light transmittance, refractive index and the like, are nearly consistent compared with glass, so that the biological cell culture dish can be used for subsequent processing, such as a fluorescence microscope, a phase contrast microscope, a confocal microscope, a differential interference contrast microscope, living cell imaging and Fluorescence In Situ Hybridization (FISH), and can ensure the subsequent processing effect;
4. the bottom of the culture dish is provided with the through hole, so that observation cannot be influenced by the thickness of the bottom of the culture dish during microscopic observation.
In conclusion, the cell culture dish can ensure cell attachment and extension, has good sealing property and air permeability, is convenient for cell observation due to the design of the through hole at the bottom of the dish, and has obvious technical progress in the field.
Drawings
FIG. 1 is a front view of one embodiment of the present invention.
Fig. 2 is a front view of another embodiment of the present invention.
FIG. 3 is a longitudinal cross-sectional view of the capsule bottom according to one embodiment of the present invention.
Figure 4 is a longitudinal cross-sectional view of a capsule bottom according to another embodiment of the invention.
Figure 5 is a top view of the capsule of the present invention.
Fig. 6 is a schematic view of the junction of the capsule body of the present invention.
Detailed Description
Example 1
The high stability surface biological cell culture dish of the invention as shown in fig. 1, 3, 5 and 6 comprises: a dish body 12, a dish cover 11 and a climbing sheet 13; a square depressed area 15 is arranged in the middle of the bottom surface of the dish body 12, a round through hole 14 is arranged in the middle of the depressed area 15, the shape and the thickness of the climbing piece 13 are the same as those of the depressed area 15, the climbing piece is fixed on the depressed area, and the upper surface of the climbing piece 13 is subjected to plasma treatment; the upper edge of the dish body 12 and the lower edge of the dish cover 11 are provided with staggered bulges 16 and depressions 17 which are uniformly distributed, and the bulges 16 and the depressions 17 are the same in shape; a ditch 61 is arranged on the upper edge of the dish body 12, a protruding part corresponding to the ditch 61 is arranged on the lower edge of the dish cover 11, and the height and the width of the protruding part are consistent with the depth and the width of the ditch 61.
More specifically, the horizontal projection of the protrusion 16 is trapezoidal, and the capsule 11 is rotated to the size of the required vent hole when in use.
More specifically, climbing piece 13 adopts the APET material to make, just sunken area 15's both sides upper portion, there is the separation blade 21 of inside extension, separation blade 21 with the ware body 12 is injection moulding as an organic whole, because the APET material is softer during the installation, can withstand separation blade 21 lower extreme with climbing piece 13 on one side, under the separation blade 21 of opposite side is gone into to climbing piece 13 another side card.
More specifically, the groove 61 is fixed with the gel ring 51 for preventing sliding and effectively sealing, and the dish cover can rotate to any position for stopping due to the static friction force, so as to achieve the purpose of adjusting the size of the vent channel.
Example 2
The high stability surface biological cell culture dish of the invention as shown in fig. 2, 4, 5 and 6 comprises: a dish body 12, a dish cover 11 and a climbing sheet 13; a square depressed area 15 is arranged in the middle of the bottom surface of the dish body 12, a round through hole 14 is arranged in the middle of the depressed area 15, the shape and the thickness of the climbing piece 13 are the same as those of the depressed area 15, the climbing piece is fixed on the depressed area, and the upper surface of the climbing piece 13 is subjected to plasma treatment; the upper edge of the dish body 12 and the lower edge of the dish cover 11 are provided with staggered bulges 16 and depressions 17 which are uniformly distributed, and the bulges 16 and the depressions 17 are the same in shape; a ditch 61 is arranged on the upper edge of the dish body 12, a protruding part corresponding to the ditch 61 is arranged on the lower edge of the dish cover 11, and the height and the width of the protruding part are consistent with the depth and the width of the ditch 61.
More specifically, the horizontal projection of the protrusion 16 is rectangular, and when the dish cover 11 needs to be lifted, the convex portions of the dish cover 11 and the dish body 12 are opposite to each other.
More specifically, the climbing sheet 13 is made of organic glass, the edges of the depressed area 15 and the climbing sheet 13 are sealed by transparent glue or glass glue or wax or hot melt, the longitudinal section of the climbing sheet 13 is trapezoidal, one base angle is obliquely cut off, the included angle between one oblique edge and the base is 80-85 degrees, the included angle between the other oblique edge and the base is 100-135 degrees, the included angle between the other oblique edge and the upper edge is 100-135 degrees, the shape of the longitudinal section of the depressed area 15 is identical to that of the longitudinal section of the climbing sheet 13, the shape design of the climbing sheet and the depressed area can enable the climbing sheet with higher elastic modulus to be conveniently clamped into the depressed area during production and processing, subsequent edge sealing processing is performed, and the dish body 12 is integrally injection molded.
More specifically, the trench 61 is fixed with a silicone ring 51 for preventing sliding and effectively sealing.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. A high stability surface biological cell culture dish which characterized in that: comprises a dish body, a dish cover and a climbing sheet; a polygonal sunken area is arranged in the middle of the bottom surface of the dish body, a through hole is arranged in the middle of the sunken area, the shape and the thickness of the climbing sheet are the same as those of the sunken area, the climbing sheet is fixed on the sunken area, and the upper surface of the climbing sheet is subjected to plasma treatment; the upper edge of the dish body and the lower edge of the dish cover are provided with staggered convex and concave structures which are uniformly distributed, and the shapes of the convex and concave structures are the same; the upper edge of the dish body is provided with a ditch, the lower edge of the dish cover is provided with a protruding part corresponding to the ditch, and the height and the width of the protruding part are consistent with the depth and the width of the ditch.
2. The high stability surface biological cell culture dish of claim 1, wherein: the horizontal projection of the protrusion is in a wave trough shape of a rectangle, a trapezoid or a sine wave.
3. The high stability surface biological cell culture dish of claim 2, wherein: the climbing piece is made of APET materials.
4. The high stability surface biological cell culture dish of claim 2, wherein: the climbing sheet is made of organic glass.
5. The high stability surface biological cell culture dish of claim 3, wherein: and the upper parts of two sides of the depressed area are provided with inwards extending blocking pieces.
6. The high stability surface biological cell culture dish of claim 4, wherein: the edges of the sunken area and the climbing sheet are sealed by transparent glue or glass glue or wax or hot melt.
7. The high-stability surface biological cell culture dish according to claim 5 or 6, wherein: and a rubber ring or a gel ring or a silica gel ring is fixed in the ditch.
8. The high stability surface biological cell culture dish of claim 5, wherein: the baffle plate and the dish body are integrally formed by injection molding.
9. The high stability surface biological cell culture dish of claim 6, wherein: the vertical section of the climbing piece is trapezoidal, one bottom angle is obliquely cut off, the included angle between one oblique edge and the bottom edge is 80-85 degrees, the included angle between the other oblique edge and the bottom edge is 100-135 degrees, the included angle between the other oblique edge and the upper edge is 100-135 degrees, the shape of the vertical section of the depressed area is identical to that of the vertical section of the climbing piece, and the dish body is integrally injection-molded.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010938895.9A CN112080429A (en) | 2020-09-09 | 2020-09-09 | High stability surface biological cell culture dish |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010938895.9A CN112080429A (en) | 2020-09-09 | 2020-09-09 | High stability surface biological cell culture dish |
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| Publication Number | Publication Date |
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| CN112080429A true CN112080429A (en) | 2020-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010938895.9A Pending CN112080429A (en) | 2020-09-09 | 2020-09-09 | High stability surface biological cell culture dish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094745A (en) * | 2020-09-09 | 2020-12-18 | 江苏益玛生物科技有限公司 | Processing method of high-stability surface biological cell culture dish |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2079189U (en) * | 1990-03-30 | 1991-06-19 | 郑州铁路局西安中心防疫站 | Microbe culture dish |
| CN202175676U (en) * | 2011-08-22 | 2012-03-28 | 唐亚军 | Biochemical culture dish |
| CN203048957U (en) * | 2013-01-08 | 2013-07-10 | 复旦大学 | Slide for cell hierarchical culture |
| CN203238261U (en) * | 2013-05-21 | 2013-10-16 | 中国人民解放军南京军区南京总医院 | Cell culture dish specially used for cell climbing piece |
| CN210048783U (en) * | 2019-03-05 | 2020-02-11 | 南京诺艾新生物技术有限公司 | Cell culture dish for IM-9 cell strain construction |
| CN110791416A (en) * | 2019-11-11 | 2020-02-14 | 浙江赛宁生物科技有限公司 | Culture dish and culture dish manufacturing method |
-
2020
- 2020-09-09 CN CN202010938895.9A patent/CN112080429A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2079189U (en) * | 1990-03-30 | 1991-06-19 | 郑州铁路局西安中心防疫站 | Microbe culture dish |
| CN202175676U (en) * | 2011-08-22 | 2012-03-28 | 唐亚军 | Biochemical culture dish |
| CN203048957U (en) * | 2013-01-08 | 2013-07-10 | 复旦大学 | Slide for cell hierarchical culture |
| CN203238261U (en) * | 2013-05-21 | 2013-10-16 | 中国人民解放军南京军区南京总医院 | Cell culture dish specially used for cell climbing piece |
| CN210048783U (en) * | 2019-03-05 | 2020-02-11 | 南京诺艾新生物技术有限公司 | Cell culture dish for IM-9 cell strain construction |
| CN110791416A (en) * | 2019-11-11 | 2020-02-14 | 浙江赛宁生物科技有限公司 | Culture dish and culture dish manufacturing method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112094745A (en) * | 2020-09-09 | 2020-12-18 | 江苏益玛生物科技有限公司 | Processing method of high-stability surface biological cell culture dish |
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