CN203048957U

CN203048957U - Slide for cell hierarchical culture

Info

Publication number: CN203048957U
Application number: CN 201320009795
Authority: CN
Inventors: 周密; 姜民
Original assignee: Fudan University
Current assignee: Fudan University
Priority date: 2013-01-08
Filing date: 2013-01-08
Publication date: 2013-07-10
Anticipated expiration: 2023-01-08

Abstract

The utility model belongs to the technical field of microculture instruments, and relates to a slide for cell hierarchical culture. The slide is provided with a folded part comprising a turnup edge slide and a folded corner slide; the hemming slide consists of a plane and turnup edges; the turnup edges are arranged on two sides of the plane; the folded corner slide consists of a plane and folded corners; the folded corners are arranged at four corners of the plane; the turnup edge slide and the folded corner slide are formed by performing thermal deformation, blistering or pressing and sticking on glass, organic glass or non-toxic plastic; and the folding directions of the turnup edge slide and the folded corner slide are the same. A result shows that the slide for the cell hierarchical culture is easy to prepare and convenient to use and can be repeatedly used; the folding direction and the thickness are consistent; the slide is convenient to clamp; the experiment operations of nerve cell culture and the cell hierarchical culture are greatly simplified.

Description

A kind of slide for cell layering cultivation

Technical field

The utility model belongs to microorganism culturing technique with the apparatus field, relates to a kind of slide for cell layering cultivation; This slide comprises crimping slide and volume angle slide, is applicable to neuronal cell cultures and other kind cell layering cultivation.

Technical background

At present, along with viable cell imaging technique in the microbe research field is growing, impel at all kinds of to dynamic observe the key experiment that becomes the bio-science research from the separate tissue cultured cells, especially crucial especially to the observation of wherein each class neuronal cell.Practice shows, very difficult about the cultivation of the neuronal cell of forming mammalian central nervous system, its structure height specialization is also very fastidious to culture condition, only could survive and grow in non-neuron neurogliocyte layer usually; And, in this kind culture, neurogliocyte is more than neuronal cell, usually after with fluorescence labeled cell, the fluorescence that neurogliocyte sends is brighter than neuronal cell, makes the difficult neuronal cell to mixed culture of investigator carry out imaging and analyze its activity.The neurone that people such as Banker set up and spongiocyte layering co-culture method have overcome the spongiocyte uppity problem of growing, its result has shown that described neurone and spongiocyte are grown on the Different Plane of same extracellular fluid, the purifying of having got back when neurone obtains to stablize the spongiocyte support, but aforesaid method remains in weak point, wherein need the wax point as upholder, and each paraffin thickness of laying can't be consistent; Simultaneously, the paraffin that adopts needs to melt in advance, and the wax point need can use behind illumination-based disinfection under the ultraviolet lamp; Therefore all, cause the preliminary preparation of aforesaid method many and loaded down with trivial details.

Therefore, pressing for a kind of novel experiment equipment in the cells involved layering culture experiment helps simplify the layering of neurone spongiocyte and cultivates altogether.

Summary of the invention

The purpose of this utility model is defective and the deficiency that overcomes prior art, and a kind of utensil of microorganism culturing is provided, and is specifically related to a kind of slide for cell layering cultivation; This slide comprises crimping and volume angle slide, can simplify neurone spongiocyte layering co-culture experiments, can replace needing in the prior art to lay in advance the cover glass of paraffin and sterilization, is applicable to neuronal cell cultures and other kind cell layering cultivation.

Slide for cell layering cultivation of the present utility model is characterized in that described slide is provided with the convolution part, and it comprises crimping slide and volume angle slide;

Described crimping slide is made up of plane 1 and crimping 2, and crimping 2 is arranged on the both sides on plane 1;

Described volume angle slide is made up of plane 1 and volume angle 3, and volume angle 3 is arranged on the place, four angles on plane 1.

In the utility model, described crimping slide is made both sides towards the slide with one side convolution for adopting glass, synthetic glass, non-toxic plastic materials such as (as polyvinyl chloride) through thermal distortion, plastic uptake or compacting, the method for pasting; Among the embodiment of the present utility model, the convolution height of described crimping 2 is 1-2mm, and this crimping 2 has two, and the convolution direction is identical; When convolution slide was down placed horizontal plane, its plane kept level substantially;

In the utility model, described volume angle slide is made four angles towards the slide with one side convolution for adopting glass, synthetic glass, non-toxic plastic materials such as (as polyvinyl chloride) through thermal distortion, plastic uptake or compacting, the method for pasting; Among the embodiment of the present utility model, the convolution height at described volume angle 3 is 1-2mm, and it is identical that this volume angle 3 has four, convolution direction; When convolution slide was down placed horizontal plane, its plane kept level substantially.

During use, place culture dish to carry out the neurone cultivation after some days up the convolution of the utility model crimping slide or volume angle slide, described slide upset is made crimping or volume angle down, and transfer in the culture dish that contains spongiocyte, make neurone towards spongiocyte, carry out neuronal cell cultures and cultivate.

Use is the result show, crimping slide of the present utility model or volume angle slide preparation are simple, easy to use, and its convolution direction, consistency of thickness make things convenient for clamping, and be reusable, greatly simplified neuronal cell cultures and cell layering culture experiment.

For the ease of understanding, below will describe in detail the slide of cultivating for the cell layering of the present utility model by concrete drawings and Examples, it needs to be noted, specific examples and accompanying drawing only are in order to illustrate, obviously those of ordinary skill in the art can illustrate according to this paper, in scope of the present utility model, the utility model is made various corrections and change, these corrections and change, these corrections and change are also included in the utility model scope.

Description of drawings

Fig. 1 is a kind of crimping slide surface structure synoptic diagram for cell layering cultivation;

Fig. 2 is a kind of volume angle slide surface structure synoptic diagram for cell layering cultivation;

Fig. 3 is that the utility model places culture dish to carry out the synoptic diagram that layering is cultivated altogether;

In Fig. 1 ~ 3,1 is that plane, 2 is that crimping, 3 is the volume angle.

Embodiment

Embodiment 1

As Fig. 1, shown in Figure 3, this is used for the slide that the cell layering is cultivated, and is made up of plane 1 and crimping 2, and the both sides on described plane 1 are provided with crimping 2; Described crimped lid slide is made both sides towards the slide with one side convolution for adopting glass, synthetic glass, non-toxic plastic materials such as (as polyvinyl chloride) through thermal distortion, plastic uptake or compacting, the method for pasting;

The convolution height of crimping 2 is 1-2mm, and it is identical that this crimping 2 has two, convolution direction; When convolution slide was down placed horizontal plane, its plane kept level substantially;

During use, place culture dish to carry out the neurone cultivation after some days up folding crimping slide convolution described in the utility model down, with the upset of described slide and transfer in the culture dish that contains spongiocyte, crimping down at this moment, make neurone towards spongiocyte, carry out neuronal cell cultures.

Embodiment 2

As Fig. 2, shown in Figure 3, this is used for the slide that the cell layering is cultivated, and is made up of plane 1 and volume angle 3, establishes volume angle 3 for four jiaos of described plane 1.

Described volume angle slide is made four jiaos towards the slide with one side convolution for adopting glass, synthetic glass, non-toxic plastic materials such as (as polyvinyl chloride) through thermal distortion, plastic uptake or compacting, the method for pasting;

The convolution height at volume angle 3 is 1-2mm, and it is identical that this volume angle 3 has four, convolution direction; When convolution slide was down placed horizontal plane, its plane kept level substantially.

During use, place culture dish to carry out the neurone cultivation after some days up folding volume described in the utility model angle cover glass convolution down, with the upset of described slide and transfer in the culture dish that contains spongiocyte, roll up the angle down this moment, make neurone towards spongiocyte, carry out neuronal cell cultures.

The result of above-described embodiment shows that it is simple, easy to use, reusable that the utility model prepares, and its convolution direction, consistency of thickness make things convenient for clamping, can simplify the operation of neuronal cell cultures and cell layering culture experiment.

Claims

1. one kind is used for the slide that the cell layering is cultivated, and it is characterized in that described slide is provided with the convolution part, and it comprises crimping slide and volume angle slide;

Described crimping slide is made up of plane (1) and crimping (2), and described crimping (2) is arranged on the both sides of plane (1);

Described volume angle slide is made up of plane (1) and volume angle (3), and described volume angle (3) is arranged on the place, four angles on plane (1).

2. by the described slide for cell layering cultivation of claim 1, it is characterized in that the convolution height of described crimping (2) is 1-2mm.

3. by claim 1 or 2 described slides for cell layering cultivation, it is characterized in that its convolution direction of described crimping (2) is identical.

4. by the described slide for cell layering cultivation of claim 1, it is characterized in that the convolution height at described volume angle (3) is 1-2mm.

5. by claim 1 or 4 described slides for cell layering cultivation, it is characterized in that described volume angle (3) has four, the convolution direction is identical.