CN202471455U - Root system secretion normal position collecting device and system - Google Patents

Root system secretion normal position collecting device and system Download PDF

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Publication number
CN202471455U
CN202471455U CN2011205570605U CN201120557060U CN202471455U CN 202471455 U CN202471455 U CN 202471455U CN 2011205570605 U CN2011205570605 U CN 2011205570605U CN 201120557060 U CN201120557060 U CN 201120557060U CN 202471455 U CN202471455 U CN 202471455U
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China
Prior art keywords
root
sampling
hole
original
gathering
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Expired - Fee Related
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CN2011205570605U
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Chinese (zh)
Inventor
刘庆
肖娟
李宇飞
尹华军
陈建中
陈劲松
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Chengdu Institute of Biology of CAS
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Chengdu Institute of Biology of CAS
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Abstract

The utility model discloses a plant root system secretion normal position collecting device and a system, aims at the defects of a static culture device in the prior art, and provides an experimental apparatus which is installed on a plant growth normal position site and can collect a plant root system secretion and system. The plant root system secretion normal position collecting device comprises a collecting pipe, wherein the upper part of the collecting pipe is provided with an opening and is provided with a sampling plug, the lower part of the collecting pipe is provided with a sample outlet port connected with a sample outlet pipe, a glass bead layer is filled in the upper part of the collecting pipe, a glass wool layer is filled in the lower part of the collecting pipe, the sampling plug is made of a silicon rubber material, the sampling plug is provided with a sample inlet port and a root hole, a silicon rubber material of the periphery of the root hole is thinner so as to form a thin layer region, and the edge of from the root hole to the sampling plug is provided with a radial notch. The utility model also provides the root system secretion normal position collection system which is composed of a sampling device, a root system secretion normal position collecting device and a suction filtration device through connection. The plant root system secretion normal position collecting device and the system provided by the utility model farthest reduce the disturbance and damage of a root system of the device, can avoid generating human errors in a experimentation, can greatly shorten suction filtration time, can improve sampling efficiency, and is convenience in operation.

Description

A kind of root exudates original position gathering-device and system
Technical field
The utility model relates to a kind of gathering-device, particularly relates to a kind of secretions from plant roots original position gathering-device, belongs to experiment mechanical hook-up field.
Background technology
Plant is imported organic in soil through root system and The litter, be two kinds of main paties that influence forest ecosystem under ground portion material cycle and energy Flow.Secretions from plant roots is that root system of plant passes through the inorganic ions of rhizospheric environment release and the general designation of organic compound in the vital movement process; Circulation has important regulation to root exudates to soil nutrient through influencing the rhizosphere microorganism process; This is most important for keeping forest-tree growth and ecosystem stability, and the forest ecosystem type meaning of especially low nutrient availability is more great.Therefore, the secretion secreted in the habitat in position of secretions from plant roots, particularly plant is the important content of the underground ecological process research in land.
A kind of static culture device (Richard et al is disclosed in the prior art; 2008); This device adopts carbon-free nutrient solution completely not damage to put in the glass device that sterile glass beads and carbon-free nutrient solution are housed after soil rinses out with " cultivations " adhering to of radicula system to be cultivated, cultivate finish after employing vacuum pump suction filtration backwashing manner root exudates is washed out.There are following 5 point defects in use in this device: (1) this device upper end adopts the rubber septum that has V-cut to bind, and seals film.Adopt syringe needle repeatedly to prick partition injection nutrient solution during suction filtration, because selected root system diameter does not wait at 0.25mm~2mm, V-cut is difficult for effectively regulating; The rubber septum thickness does not clearly demonstrate simultaneously, influences device and uses; And especially occur chapping after the rubber material is used repeatedly, be clamminess, harden, soften in the summer of high temperature, phenomenons such as efflorescence, variable color, mildew; (2) the device lower end adopts the grid funnel to prevent that beaded glass from stopping up outlet, yet the syringe lower end of China outlet at present can not can't be fixed on the common common syringe that needle holder is positioned at the syringe side by the grid funnel all at side; (3) structure of similar threeway is adopted in device exit, lower end, and finding in the operation is prone to come off causes collecting flow and lose, and is prone to puncture aluminium foil and the pollution that causes receiving soil; (4) parcel one deck aluminium foil is cultivated in the The litter of nuzzling, yet this length of root system is in soil, and existing will be installed the loam cake The litter, and possibly change the living environment of root system; (5) during suction filtration, dismantle earlier, open aluminium foil and prick with syringe and add the nutrient solution suction filtration after passing through rubber septum and sealing film, change after the end again and seal film and moistening thieving paper, wrap aluminium foil lid The litter, the whole operation process causes sample contamination easily.
The prior art list of references:
Richard?P.Phillips*,Yael?Erlitz,Raven?Bier?and?Emily?S.Bernhardt.New?approach?for?capturing?soluble?root?exudates?in?forest?soils.Functional?Ecology?2008,22,990-999.
The utility model content
The purpose of the utility model is exactly the deficiency to prior art, provides a kind of and can be installed in experimental provision and the system that massive planting thing root exudates is checked and accepted in the plant growth in situ.
For realizing above-mentioned purpose, the technical scheme of the utility model is following:
A kind of root exudates original position gathering-device comprises collection tube, and the collection tube upper opening is also installed the sampling plug, and the bottom has outlet and connects the appearance pipe, and the collection tube internal upper part is loaded glass bead layer, and the glass wool layer is loaded in the bottom; Said sampling has injection port and root hole beyond the Great Wall, it is characterized in that: said sampling plug is that silastic material is processed.
The sampling plug adopts silastic material to process in the said apparatus, effectively utilizes the good characteristic of silastic material sealing effectiveness, makes the sampling plug can make things convenient for installing/dismounting and difficult drop-off.And injection port beyond the Great Wall and root hole can experience use repeatedly and be difficult for chapping, be clamminess, harden, soften, efflorescence, guaranteed that sample is difficult for the contaminated while and has reduced installation cost again.
Said apparatus adopts the injector for medical purpose syringe as collection tube, with syringe needle as outlet.The sampling plug is installed in injector syringe upper opening place, average thickness 4~5mm, root hole diameter 0.5~2mm.Root hole has radial slot to sampling plug edge.The attenuation of root hole periphery silastic material forms the thin layer district, thin layer district diameter 5~10mm, average thickness 0.2~0.3mm.Syringe specifically can adopt 30ml or 50ml specification; When gatherer adopted the 30ml syringe, glass bead layer floor height 10.5~11.5cm was when gatherer adopts the 50ml syringe; Glass bead layer floor height 12~13cm, beaded glass are particle diameter<1mm and through the beaded glass of 3M salt pickling; The high layer by layer 0.5~1cm of glass wool adopts the glass wool through the pickling of 3M salt.
Be the basis with above-mentioned root exudates original position gathering-device, the utility model also provides a kind of root exudates original position collection system, and concrete technical scheme is following:
A kind of root exudates original position collection system is characterized in that: comprise root exudates original position gathering-device, said original position gathering-device injection port is inserted with the sample introduction pipe, and the sample introduction pipe connects sampling device; Said outlet is with out the appearance pipe, goes out the appearance pipe and connects the suction filtration device.
In the said system, injection port and sample introduction pipe junction, outlet and go out an appearance pipe junction silicon rubber web member all is installed are to reach sealing effectiveness.Sampling device adopts injector for medical purpose.The suction filtration device comprises bottle,suction, surge flask, vacuum pump, and bottle,suction and surge flask all are sealing polypropylene empty bottles that the top has air admission hole and aspirating hole.Go out appearance pipe and connect a bottle,suction air admission hole, be connected silicone tube between bottle,suction aspirating hole and the surge flask air admission hole, be connected silicone tube between surge flask aspirating hole and vacuum pump.The effect of bottle,suction is the secretions from plant roots that the splendid attire suction filtration goes out, and the surge flask effect is to prevent that solution from getting into vacuum pump, with the protection vacuum pump.
Compared with prior art; The beneficial effect of the utility model is: (1) has adopted special-purpose silastic material to make the sampling plug, and thin layer district quality is soft, sampling plug also easy damaged root system not when extruding; Sillicon rubber blocking also has excellent electric insulating, oxytolerant resistance to aging, fast light resistance to aging and mildew resistance, chemical stability etc.; Can prevent the root exudates sample contamination, and can after cleaning, recycle, reduce cost of products; (2) glass wool is high layer by layer is to confirm through repetition test, and no latch up phenomenon takes place in the warranty test; (3) mouth of pipe junction is installed silicon rubber web member or silicone rubber seal, flexible operation, difficult drop-off as required; (4) gathering-device and system use easy operating, save time, and raise the efficiency, and do not damage the root system in the disturbance device not, the introducing of also stopping pollutant basically.
Description of drawings
Fig. 1 is a root exudates original position gathering-device structural representation.
Fig. 2 is a sampling plug structure synoptic diagram.
Fig. 3 is a root exudates original position collection system structural representation.
Label is following among the figure:
1 root exudates original position gathering-device, 11 collection tubes, 12 sampling plugs
121 injection ports, 122 root holes, 123 thin layer districts
124 radial slots, 13 outlets 14 go out the appearance pipe
15 glass bead layer, 16 glass wool layers, 17 sample introduction pipe
18 silicon rubber web members, 19 silicone rubber seal, 2 sampling devices
31 bottle,suctions, 32 surge flasks, 33 vacuum pumps
34 silicone tubes
Embodiment
Below in conjunction with accompanying drawing, the preferred embodiment of the utility model is done further to describe.
Embodiment one
Like Fig. 1~shown in Figure 2, make a kind of root exudates original position gathering-device.
Fig. 1 is a root exudates original position gathering-device structural representation.Root exudates original position gathering-device comprises collection tube 11, and collection tube 11 upper openings are also installed sampling plug 12.Sampling plug 12 is that silastic material is processed, and has injection port 121 and root hole 122 on the sampling plug 12; Collection tube 11 bottoms have outlet 13.Collection tube 11 is 30ml or 50ml specification injector for medical purpose syringe, and outlet 13 is syringe needle.
Collection tube 11 internal upper parts are loaded glass bead layer 15, and glass wool layer 16 is loaded in the bottom.Glass bead layer 15 floor heights 10.5~11.5cm in the 30ml specification syringe, glass bead layer floor height 12~13cm in the 50ml specification syringe, beaded glass particle diameter<1mm.Glass wool layer 16 floor height 5~10mm.
Fig. 2 is a sampling plug structure synoptic diagram.Root hole 122 periphery silastic material attenuation form thin layer district 123, and root hole 122 has radial slot 124 to sampling plug 12 edges.Sampling plug 12 average thicknesss 4~5mm, root hole 122 diameters 0.5~2mm, thin layer district 123 diameters, 5~10mm, average thickness 0.2~0.3mm.
Embodiment two
As shown in Figure 3.Make a kind of root exudates original position collection system, its place identical with embodiment one is repeated description no longer, and difference is:
Fig. 3 is a root exudates original position collection system structural representation.Root exudates original position collection system comprises root exudates original position gathering-device 1, and original position gathering-device 1 injection port 121 is inserted with sample introduction pipe 17, and the sample introduction pipe connects sampling device 2, and sampling device 2 is injector for medical purpose.Outlet 13 is with out appearance pipe 14, goes out appearance pipe 14 and connects the suction filtration device, and the suction filtration device comprises bottle,suction 31, surge flask 32, vacuum pump 33, and bottle,suction 31 all is sealing empty bottles that the top has air admission hole and aspirating hole with surge flask 32.Be connected appearance pipe 14 between outlet 13 and bottle,suction 31 air admission holes, be connected silicone tube 34 between bottle,suction 31 aspirating holes and surge flask 32 air admission holes, be connected silicone tube 34 between surge flask 32 aspirating holes and vacuum pump.
Sample introduction pipe 17 or go out appearance pipe 13 junctions silicon rubber web member 18 is arranged in the system is to reach sealing effectiveness.
Original position gathering-device 1 tilts to place, and is 45 °~90 ° with the ground angle.
Root exudates original position collection system method of application is: load glass wool layer 7 and glass bead layer 6 in the collection tube 11, sample introduction pipe 17 inserts sample holes 121, and sample introduction pipe upper end is with silicone rubber seal 19 sealings; Root system of plant is put root hole 122 into through radial slot 124; Sampling plug 12 is installed on the collection tube 11, and root system of plant is placed into glass bead layer and cultivates; Collection tube 11 outlets 13 connect appearance pipe 14, go out appearance pipe 14 lower ends with silicone rubber seal 19 sealings.The original position gathering-device 1 outer aluminium foil of wrapping up in tilts to be placed in the soil, is 45 °~90 ° with the ground angle, and the best is 60 °, buries original position gathering-device 1 with fine earth and cultivates.After cultivating end, take off each silicone rubber seal 19, sample introduction pipe 17 connects sampling devices 2, and sampling device 2 is filled with carbon-free nutrient solution, goes out appearance pipe 14 and connects the suction filtration device, carries out suction filtration.Each sampler 1 corresponding bottle,suction 31, the splendid attire root exudates is collected liquid, and bottle,suction 31 adopts public bottle cap, then changes bottle,suction 31 when changing sampler 1.Flow velocity is set is about 5cm to vacuum pump 33 during suction filtration 3Se -1

Claims (10)

1. root exudates original position gathering-device; Comprise collection tube (11), collection tube (11) upper opening is also installed sampling plug (12), and the bottom has outlet (13) and connects appearance pipe (14); Collection tube (11) internal upper part is loaded glass bead layer (15), and glass wool layer (16) is loaded in the bottom; Have injection port (121) and root hole (122) on the said sampling plug (12), it is characterized in that: said sampling plug (12) is that silastic material is processed.
2. device according to claim 1 is characterized in that: the attenuation of said root hole (122) periphery silastic material forms thin layer district (123), and root hole (122) has radial slot (124) to sampling plug (12) edge.
3. device according to claim 2 is characterized in that: said collection tube (11) is the injector for medical purpose syringe, and outlet (13) is a syringe needle.
4. device according to claim 3 is characterized in that: said sampling plug (12) average thickness 4~5mm, root hole (122) diameter 0.5~2mm, thin layer district (123) diameter 5~10mm, average thickness 0.2~0.3mm.
5. device according to claim 4 is characterized in that: said syringe is 30ml or 50ml specification.
6. device according to claim 5 is characterized in that: said glass bead layer (15) beaded glass particle diameter<1mm, glass bead layer (15) floor height 10.5~11.5cm in the 30ml specification syringe, or glass bead layer (15) floor height 12~13cm in the 50ml specification syringe; Said glass wool layer (16) floor height 5~10mm.
7. root exudates original position collection system of utilizing the arbitrary described root exudates original position gathering-device of claim 1~6 to form; It is characterized in that: comprise root exudates original position gathering-device (1); Said original position gathering-device (1) injection port (121) is inserted with sample introduction pipe (17), and sample introduction pipe (17) connects sampling device (2); Said outlet (13) is with out appearance pipe (14), goes out appearance pipe (14) and connects the suction filtration device.
8. according to the said system of claim 7, it is characterized in that: sample introduction pipe (17) or go out appearance pipe (a 13) junction silicon rubber web member (18) is arranged in the system.
9. according to claim 7 or 8 described systems, it is characterized in that: said sampling device (2) is an injector for medical purpose; Said suction filtration device comprises bottle,suction (31), surge flask (32), vacuum pump (33); Bottle,suction (31) all is sealing empty bottles that the top has air admission hole and aspirating hole with surge flask (32); Be connected appearance pipe (14) between outlet (13) and bottle,suction (31) air admission hole; Be connected silicone tube (34) between bottle,suction (31) aspirating hole and surge flask (32) air admission hole, be connected silicone tube (34) between surge flask (32) aspirating hole and vacuum pump.
10. according to the said system of claim 9, it is characterized in that: said root exudates original position gathering-device (1) tilts to place when sampling, is 45 °~90 ° with the ground angle.
CN2011205570605U 2011-12-28 2011-12-28 Root system secretion normal position collecting device and system Expired - Fee Related CN202471455U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103776662A (en) * 2014-01-22 2014-05-07 福建农林大学 Collecting device and method of root exudates from xerophyte
CN105300737A (en) * 2015-05-08 2016-02-03 中国科学院沈阳应用生态研究所 Long-term in-situ collection method and device for forest tree root exudates
CN106053135A (en) * 2016-05-10 2016-10-26 中国科学院成都生物研究所 A grassland herbal plant root exudate in-situ collecting method
CN109765077A (en) * 2019-02-28 2019-05-17 中国科学院南京土壤研究所 A kind of root exudates sampler and sampling system
CN113514269A (en) * 2021-06-03 2021-10-19 海南大学 Root exudate normal position collection system

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103776662A (en) * 2014-01-22 2014-05-07 福建农林大学 Collecting device and method of root exudates from xerophyte
CN103776662B (en) * 2014-01-22 2016-01-27 福建农林大学 A kind of xerophyte root exudates gathering-device and collection method thereof
CN105300737A (en) * 2015-05-08 2016-02-03 中国科学院沈阳应用生态研究所 Long-term in-situ collection method and device for forest tree root exudates
CN106053135A (en) * 2016-05-10 2016-10-26 中国科学院成都生物研究所 A grassland herbal plant root exudate in-situ collecting method
CN109765077A (en) * 2019-02-28 2019-05-17 中国科学院南京土壤研究所 A kind of root exudates sampler and sampling system
CN113514269A (en) * 2021-06-03 2021-10-19 海南大学 Root exudate normal position collection system
CN113514269B (en) * 2021-06-03 2022-03-04 海南大学 Root exudate normal position collection system

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20121003

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CF01 Termination of patent right due to non-payment of annual fee