CN2863808Y - Cell multiple-grooved incubator - Google Patents
Cell multiple-grooved incubator Download PDFInfo
- Publication number
- CN2863808Y CN2863808Y CN 200520022169 CN200520022169U CN2863808Y CN 2863808 Y CN2863808 Y CN 2863808Y CN 200520022169 CN200520022169 CN 200520022169 CN 200520022169 U CN200520022169 U CN 200520022169U CN 2863808 Y CN2863808 Y CN 2863808Y
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- China
- Prior art keywords
- cell
- cell body
- groove
- grooved
- incubator
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- Expired - Fee Related
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Abstract
A cell multiple-grooved incubator is disclosed. The main body comprises a groove body and a cover glass, which is characterized in that a plurality of square culture grooves connected up and down are set uniformly on the groove body, a fillister with a certain thickness is set on the bottom of the culture grooves, the cover glass buckles into the fillister and forms a plane with the inferior bottom surface of the groove, a slideway is set on the upside of the groove and the cover glass can slide freely in the slideway of the groove. The design has the advantages of a simple structure, convenient to process and a graceful appearance. The utility model provides a new incubator with the integration of the cell multiple-grooved culture and the living observation function.
Description
Affiliated technical field
The utility model belongs to a kind of cell culture apparatus, particularly a kind of novel incubator that integrates the cultivation of cell multiple-grooved, vivo observation function.
Background technology
Cell cultures is meant that taking out cell from in-vivo tissue imitates and occur environment in the body, under aseptic, proper temperature and potential of hydrogen and certain nutritional condition, makes the phase growth and breeding, and keeps a kind of culture technique of its 26S Proteasome Structure and Function.In modern bio-science research, usually need in the process of cell cultures, carry out a series of microscopic observation (cell levels, molecular level), therefore under maintenance cell growth conditions, carry out somatoscopy and seem especially important.Yet, present laboratory cell observation program great majority be in advance at culture dish, culture plate, shake bottle, cultivate in the test tube after, resampling places under the various light microscopics and observes.And traditional carrying glass cover glass test macro because its sealing property is bad, working solution can very fast volatilization and dry up, and makes observations distortion or some test can't carry out at all.
Summary of the invention
The purpose of this utility model is to overcome above-mentioned weak point, provide a kind of simple in structure, easy to process, external form is graceful and integrate the novel cell multiple-grooved incubator of cell cultures, vivo observation function.
In order to achieve the above object, the technical scheme that the utility model is taked is: main part is made up of cell body and cover glass two parts, it is characterized in that: cell body evenly is provided with a plurality of square culture tanks that communicate up and down, the culture tank bottom is provided with certain thickness groove, slide glass becomes a plane with the cell body bottom surface after buckling into groove, the cell body upper end is provided with slideway, and cover glass can be free to slide in the slideway on cell body.
The thickness of cell body is 30～70mm.
The square length of culture tank is 15～20mm on the cell body, and the degree of depth of groove is 0.25mm～0.5mm, and the thickness of cover glass is 0.5～1mm.
Cover glass and slide glass material are glass, and the cell body material is a synthetic glass.
The utility model has the advantages that:
1. integrate cell cultures and somatoscopy function, and the observation of cell is under the nature living condition, the result is more true and reliable.
2. a cell body is provided with a plurality of culture tanks simultaneously, can cultivate the cell of different sorts, different culture condition simultaneously, does controlled observation at microscopically by the easy switching of Stage microscope.
3. culture tank has bigger volume and better stopping property with respect to the common glass cover glass test macro that carries, and cell culture fluid (or working fluid) can not dry up because of volatilization when observing.
4. dismounting, cleaning, sterilization conveniently can repeatedly reuse.
Description of drawings
Fig. 1 is Facad structure figure of the present utility model, and Fig. 2 is its reverse side structure iron.
Embodiment
Below in conjunction with accompanying drawing utility model embodiment is described in further detail:
As seen from Figure 1, Figure 2: main part is made up of cell body 1 and cover glass 4 two parts, it is characterized in that: cell body 1 evenly is provided with a plurality of square culture tanks 3 that communicate up and down, culture tank 3 bottoms are provided with certain thickness groove 5, slide glass 6 buckles into groove 5 backs becomes a plane with cell body 1 bottom surface, cell body 1 upper end is provided with slideway 2, and cover glass 4 can be free to slide in the slideway on the cell body 12.
The thickness of described cell body 1 is 30～70mm.
The square length of culture tank 3 is 15～20mm on the described cell body 1, and the degree of depth of groove 5 is 0.25mm～0.5mm, and the thickness of cover glass 4 is 0.5～1mm.
Described cover glass 4 and slide glass 6 materials are glass, and cell body 1 material is a synthetic glass.
Principle of work of the present utility model is: at first cell multiple-grooved incubator is cleaned, sterilization, reverse side up, slide glass 6 is buckled in the groove 5 of cell body 1 bottom, around slide glass 6, evenly coat a little Vaseline then to strengthen the stopping property of groove 5 and slide glass 6, cell culture apparatus turn to face up, to treat that cultured cells and nutrient solution thereof add in the culture tank 3 with minimum demand, place in the incubator and cultivate, at the appointed time, the cell body 1 that fills culturing cell is taken out, cover glass 4 is sealed up each culture tank 3 by slideway 2, after cell body 1 is put on light microscopic (common light microscopic) Stage microscope, one of them culture tank 3 optical system for alignment is observed, moving stage, make other culture tank 3 successively optical system for alignment observe.If need carry out the cell dyeing Fluirescence observation on fluorescent microscope, laser confocal microscope, the back covered 4 that cell in the culture tank 3 can be dyeed is inserted light path and is observed according to the aforesaid operations step.
Claims (4)
1. cell multiple-grooved incubator, main part is made up of cell body (1) and cover glass (4) two parts, it is characterized in that: cell body (1) evenly is provided with a plurality of square culture tanks (3) that communicate up and down, culture tank (3) bottom is provided with certain thickness groove (5), slide glass (6) buckles into groove (5) back becomes a plane with cell body (1) bottom surface, cell body (1) upper end is provided with slideway (2), and cover glass (4) can be free to slide in the slideway (2) on the cell body (1).
2. according to the described cell multiple-grooved of claim 1 incubator, it is characterized in that: the thickness of cell body (1) is 30～70mm.
3. according to the described cell multiple-grooved of claim 1 incubator, it is characterized in that: the square length that cell body (1) is gone up culture tank (3) is 15～20mm, and the degree of depth of groove (5) is 0.25mm～0.5mm, and the thickness of cover glass (4) is 0.5～1mm.
4. according to the described cell multiple-grooved of claim 1 incubator, it is characterized in that: cover glass (4) and slide glass (6) material are glass, and cell body (1) material is a synthetic glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520022169 CN2863808Y (en) | 2005-12-19 | 2005-12-19 | Cell multiple-grooved incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520022169 CN2863808Y (en) | 2005-12-19 | 2005-12-19 | Cell multiple-grooved incubator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2863808Y true CN2863808Y (en) | 2007-01-31 |
Family
ID=37676260
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520022169 Expired - Fee Related CN2863808Y (en) | 2005-12-19 | 2005-12-19 | Cell multiple-grooved incubator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2863808Y (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226150A (en) * | 2011-05-13 | 2011-10-26 | 刘小龙 | Cell cultivating apparatus used for imaging living cell |
| CN102807952A (en) * | 2012-08-14 | 2012-12-05 | 中国人民解放军南京军区南京总医院 | Rocking dynamic culture box |
| CN103149068A (en) * | 2013-03-07 | 2013-06-12 | 珠海市丽拓发展有限公司 | Leucorrhea, defecate and urine routine microscope examination slide making kit |
| CN106249396A (en) * | 2016-09-09 | 2016-12-21 | 芜湖市科源教学设备有限公司 | A kind of coverslip that is not required to can be directly used for the teaching microscope slide of film-making |
| CN107063787A (en) * | 2016-12-19 | 2017-08-18 | 浙江世纪康大医疗科技股份有限公司 | A kind of automatic pullable cover plate system |
| CN107941591A (en) * | 2017-12-28 | 2018-04-20 | 贵州大学 | Small-sized mite class cleaning device |
-
2005
- 2005-12-19 CN CN 200520022169 patent/CN2863808Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226150A (en) * | 2011-05-13 | 2011-10-26 | 刘小龙 | Cell cultivating apparatus used for imaging living cell |
| CN102807952A (en) * | 2012-08-14 | 2012-12-05 | 中国人民解放军南京军区南京总医院 | Rocking dynamic culture box |
| CN102807952B (en) * | 2012-08-14 | 2014-08-27 | 中国人民解放军南京军区南京总医院 | Rocking dynamic culture box |
| CN103149068A (en) * | 2013-03-07 | 2013-06-12 | 珠海市丽拓发展有限公司 | Leucorrhea, defecate and urine routine microscope examination slide making kit |
| CN106249396A (en) * | 2016-09-09 | 2016-12-21 | 芜湖市科源教学设备有限公司 | A kind of coverslip that is not required to can be directly used for the teaching microscope slide of film-making |
| CN106249396B (en) * | 2016-09-09 | 2019-03-05 | 南陵旺科知识产权运营有限公司 | A kind of application method for being not required to coverslip and can be directly used for the teaching glass slide of film-making |
| CN107063787A (en) * | 2016-12-19 | 2017-08-18 | 浙江世纪康大医疗科技股份有限公司 | A kind of automatic pullable cover plate system |
| CN107941591A (en) * | 2017-12-28 | 2018-04-20 | 贵州大学 | Small-sized mite class cleaning device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |