CN102807952B - Rocking dynamic culture box - Google Patents
Rocking dynamic culture box Download PDFInfo
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- CN102807952B CN102807952B CN201210294854.6A CN201210294854A CN102807952B CN 102807952 B CN102807952 B CN 102807952B CN 201210294854 A CN201210294854 A CN 201210294854A CN 102807952 B CN102807952 B CN 102807952B
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- crank
- chute
- connecting rod
- cultivation case
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
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Abstract
The invention discloses a rocking dynamic culture box. The rocking dynamic culture box comprises a box body, a sliding groove, a motor, a reciprocating motion mechanism and a motor speed adjusting device, wherein a tray is arranged in the box body; the sliding groove is formed below the tray; a sliding block matched with the sliding groove is arranged at the bottom of the tray and connected with the sliding groove in a sliding way; the sliding groove is supported by a bracket; the reciprocating motion mechanism comprises a crank and a connecting rod; one end of the connecting rod is connected with the sliding block; the other end of the connecting rod is connected with an output shaft of the motor through the crank; the motor drives the crank to rotate and pushes the sliding block to reciprocate in the sliding groove through the connecting rod; and the motor speed adjusting device is connected with the motor. According to the rocking dynamic culture box, the crank and connecting rod mechanism is driven by the motor to drive the tray to reciprocate with a low frequency in a small range, so that an oocyte or embryo for in-vitro culture is positioned in a dynamic culture environment, so as to simulate the rocking state of the oocyte or embryo in uterus. Therefore, the embryo development quality can be remarkably promoted and the later embryo implantation can be promoted, so as to increase the pregnancy rate.
Description
Technical field
The present invention relates to biotechnology or reproductive medicine field, relate in particular to and a kind ofly can carry out to ovocyte or embryo the incubator of dynamic cultivation.
Background technology
In animal embryo engineering research or Issues of Human Assisted Reproductive Technologies, the maturation in vitro of ovocyte, the vitro culture of fertilization or preimplantation embryos is the routine operation flow process in laboratory, is mainly to carry out in incubator.
For the ectogenesis quality that improves gamete or embryo, laboratory study mainly concentrates on the optimization of medium component, and cytokine is added, suitable oxygen concn etc.Along with going deep into of laboratory seminar research, the culture system in vitro of embryo or gamete is day by day perfect, various culture condition (gas phases, temperature) simulate environment (uterus or uterine tube) in female repro ductive system completely as far as possible, but compare with natural embryonic development in body, embryo quality and implantation rate can not match in excellence or beauty completely, reason is that in female reproductive tract, the environment of fetal development is more complicated, uterine tube or intrauterine secreting liquid complicated component, provide perfect growing environment to embryo's growth and implantation.In these researchs, oocyte maturation or embryo's cultivation is mainly to carry out in static substratum.And the ripening process of ovocyte in body, and ovocyte and sperm be combined after fertilization at magnum tubae uterinae, the continuation growth of zygote, is accompanied by intratubal migration, enters uterus when morula.Therefore the ovocyte or the embryo that in body, grow are dynamic migrations, are accompanied by the daily routines of parent, not remain static always.Dynamic variation not only can be diluted embryo's metabolic waste in time, promotes the exchange of embryo's surrounding gas and biomacromolecule material simultaneously.
Someone studies and points out, during in-vitro maturity of porcine oocytes, gives the machinery concussion of certain frequency, though can not promote the core of ovocyte ripe (growing to the MII phase), the development quality of ovocyte can significantly be promoted.Same principle, in human reproduction's medical science, carries out low-frequency concussion or waves ectogenetic embryo, make it in dynamic cultivation conditions, result shows, can significantly promote embryo's development quality also can promote embryo's later stage implantation, improves pregnancy rate.Illustrate that in this training method and embryoid body, development models is more approaching.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of dynamic cultivation case that makes vibrating state in vitro culture ovocyte or embryo's simulation parent.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: a kind of dynamic cultivation case that rocks, comprise casing, and in casing, be provided with pallet, also comprise chute, motor, reciprocating device and motor speed control device;
Chute is located at pallet below, and tray bottom is provided with the slide block suitable with chute, and slide block and chute are slidably connected; Chute is by stent support;
Reciprocating device comprises crank and connecting rod, and connecting rod one end is connected with the slide block of tray bottom, and the other end is connected with motor output shaft by crank, and driven by motor crank up also promotes slide block by connecting rod and moves back and forth in chute;
Motor speed control device is connected with motor.
As preferably, also comprise timing device, timing device is controlled the break-make of motor power.
As preferably, motor is reducing motor.
As preferably, motor speed control device is electrically-actuated throttle device.
As preferably, motor speed control device is mechanical adjustable speed drive; Motor output shaft is connected with crank by mechanical adjustable speed drive.
As preferably, motor speed control device comprises control button, controls button and is located at box outer surface.
As preferably, reciprocating mechanism is located at pallet below.
As preferably, pallet card is provided with for settling the draw-in groove of culture dish;
As preferably, draw-in groove is square groove or circular groove or strip groove.
As preferably, also comprise culture dish, culture dish bottom is provided with the projection suitable with pallet draw-in groove.
The invention has the beneficial effects as follows:
By motor driving crank linkage assembly, drive pallet to do small size low frequency to-and-fro movement, make ovocyte in vitro culture or embryo in dynamic culture environment, with simulation ovocyte or the vibrating state of embryo in parent, can significantly promote embryo's development quality also can promote embryo's later stage implantation, improve pregnancy rate.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.
Fig. 1 is the front view that the present invention rocks dynamic cultivation case embodiment 1.
Fig. 2 is the vertical view that the present invention rocks dynamic cultivation case embodiment 1.
Fig. 3 is the pallet disk structure figure that the present invention rocks dynamic cultivation case embodiment 1.
In figure, 1-casing, 101-chamber door, 2-pallet, 201-draw-in groove, 3-slide block, 4-chute, 5-connecting rod, 6-crank, 7-motor, 8-motor adjusting device, 9-culture dish, 10-button, 11-output shaft.
Embodiment
Embodiment 1:
Fig. 1 is a kind of dynamic cultivation case that rocks, and comprises casing 1, and casing is provided with chamber door 101, is provided with pallet 2, chute 4, motor 7, reciprocating device and motor speed control device 8 in casing.
Chute 4 is located at pallet 2 belows, and tray bottom is provided with the slide block 3 suitable with chute 4, and pallet and slide block can be integral pieces, can be also the split part interfixing.Slide block 3 is contained in chute and can in chute, slides.Chute 4 is by frame support.Pallet 2 can be reciprocating back and forth above chute.
Motor speed control device 8 adopts electrically-actuated throttle device, and electrically-actuated throttle device is connected with motor 7.By electrically-actuated throttle device, can regulate the rotating speed of motor output shaft 11.
Reciprocating device is toggle, one end of connecting rod 5 is connected with pallet 2 bottoms, the other end is connected with crank 6, and crank is fixedly connected with the output shaft 11 of motor, and motor 7 drives cranks 6 revolutions the slide block 3 by connecting 5 promotion pallets to move back and forth chute 4 is interior.
The amplitude moving back and forth can be by regulating the preseting length of crank 6 and connecting rod 5 to regulate, and moving back and forth frequency can regulate by changing the rotating speed of motor 7.Reach like this with the oscillation form of small size low frequency and simulate the kinestate that embryo rocks in parent.
Electrically-actuated throttle device is provided with to be controlled with button 10, and button 10 is located at casing 1 outside surface (Fig. 2).
In Fig. 3, for avoiding culture dish 9 to be moved in card when the tray motion, pallet 2 cards are provided with for settling the draw-in groove 201 of culture dish 9.Set draw-in groove is square or circular, and square draw-in groove is used for arranging NUNC tetra-hole culture dish, and circular draw-in groove directly adopts the shape consistent with the circular culture dish of 35mm bottom.
The present embodiment also comprises timing device (not marking in Fig. 1), and timing device is controlled the break-make of motor power, thereby control pallet, produces intermittent to-and-fro movement.Timing device also can be by controlling pallet 2 timed motion and regularly stopping, and reaches the get up in the night to urinate daytime state of volt of simulation parent.
Motor 7, crank 6, connecting rod 5, motor speed control device 8 are installed in space in the below of frame.
The present embodiment also has other structures identical with common CO2 incubator.As CO2, N2 access port, can detected temperatures in case and the infrared probe of CO2 concentration, the damping device in incubator.
During use, the culture dish that ovocyte or embryo are housed is embedded in the draw-in groove on pallet.With button, set motor speed, and the time intermittently starting, close chamber door 101 and can start to cultivate.
With button 10 starter motors, motor 7 output shafts 11 drive crank 6 revolutions, promote pallet 2 interior reciprocating at chute 4 by connecting rod 5, realize thus pallet with the motion of oscillation on small scale form back and forth, simulation embryo or the rolling flowing mode of gamete in parent.
The present embodiment is that the CO2 incubator that routine is used improves, pallet can carry out dynamic low frequency oscillation on small scale according to frequency or the amplitude set, give a dynamic culture environment, and then can promote ovocyte or embryo's ectogenesis effect, the oocyte in vitro maturation of Erecting and improving and embryo's culture system in vitro.
Embodiment 2:
Pallet 2 cards are provided with strip-shaped clamp groove, and the culture dish bottom of using is provided with the bar shaped raised line suitable with strip groove equally.
Motor speed control device 8 adopts mechanical adjustable speed drive, and motor output shaft is connected with toggle by mechanical adjustable speed drive.By mechanical adjustable speed drive, can regulate the rotating speed of motor output shaft 11.
Other structures of the present embodiment are identical with embodiment 1.
Claims (8)
1. rock a dynamic cultivation case, comprise casing, in described casing, be provided with pallet, it is characterized in that: also comprise chute, motor, reciprocating device, motor speed control device and for simulating the get up in the night to urinate daytime timing device of state of volt of parent;
Described pallet card is provided with for settling the draw-in groove of culture dish; Described chute is located at pallet below, and tray bottom is provided with the slide block suitable with chute, and slide block and chute are slidably connected; Chute is by stent support;
Described reciprocating device comprises crank and connecting rod, and connecting rod one end is connected with the slide block of tray bottom, and the other end is connected with motor output shaft by crank, and driven by motor crank up also promotes slide block by connecting rod and moves back and forth in chute;
Described motor speed control device is connected with motor;
Described timing device is controlled the break-make of motor power.
2. the dynamic cultivation case that rocks according to claim 1, is characterized in that: described motor is reducing motor.
3. the dynamic cultivation case that rocks according to claim 1, is characterized in that: described motor speed control device is electrically-actuated throttle device.
4. the dynamic cultivation case that rocks according to claim 1, is characterized in that: described motor speed control device is mechanical adjustable speed drive; Motor output shaft is connected with crank by mechanical adjustable speed drive.
5. the dynamic cultivation case that rocks according to claim 1, is characterized in that: described motor speed control device comprises control button, controls button and is located at box outer surface.
6. the dynamic cultivation case that rocks according to claim 1, is characterized in that: described reciprocating mechanism is located at pallet below.
7. the dynamic cultivation case that rocks according to claim 1, is characterized in that: described draw-in groove is square groove or circular groove or strip groove.
8. according to rocking dynamic cultivation case described in claim 1 or 7, it is characterized in that: also comprise culture dish, described culture dish bottom is provided with the projection suitable with pallet draw-in groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210294854.6A CN102807952B (en) | 2012-08-14 | 2012-08-14 | Rocking dynamic culture box |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210294854.6A CN102807952B (en) | 2012-08-14 | 2012-08-14 | Rocking dynamic culture box |
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| CN102807952A CN102807952A (en) | 2012-12-05 |
| CN102807952B true CN102807952B (en) | 2014-08-27 |
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| CN201210294854.6A Expired - Fee Related CN102807952B (en) | 2012-08-14 | 2012-08-14 | Rocking dynamic culture box |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103645303B (en) * | 2013-12-17 | 2016-01-13 | 中国海洋大学 | A kind of experimental provision of simulating Marine shoal oil spilling |
| CN104789469B (en) * | 2015-04-29 | 2017-05-24 | 天津理工大学 | Bioreactor for in-vitro cell culture centrifugal high-acceleration loading |
| US10995312B2 (en) | 2015-08-25 | 2021-05-04 | Global Life Sciences Solutions Usa Llc | Biomanufacturing apparatus |
| CN106993525B (en) * | 2017-05-18 | 2019-09-17 | 贵州务川科华生物科技有限公司 | Paper mulberry vegetative propagation breeding apparatus |
| CN110055159B (en) * | 2019-04-28 | 2022-07-26 | 青岛农业大学 | Production equipment and method of microecologics for pet intestinal health care |
| CN111607516B (en) * | 2020-06-09 | 2021-07-09 | 苏州大学 | Early embryo oviduct-simulated environment in-vitro culture chip for breaking development retardation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2607373Y (en) * | 2003-02-26 | 2004-03-24 | 陈寅初 | Semi-automatic double-phase blood bacteria increasing apparatus |
| CN2616547Y (en) * | 2003-04-01 | 2004-05-19 | 黄海智 | Vertical photosynthetic bacterial culture apparatus |
| CN2863808Y (en) * | 2005-12-19 | 2007-01-31 | 祖述冲 | Cell multiple-grooved incubator |
-
2012
- 2012-08-14 CN CN201210294854.6A patent/CN102807952B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2607373Y (en) * | 2003-02-26 | 2004-03-24 | 陈寅初 | Semi-automatic double-phase blood bacteria increasing apparatus |
| CN2616547Y (en) * | 2003-04-01 | 2004-05-19 | 黄海智 | Vertical photosynthetic bacterial culture apparatus |
| CN2863808Y (en) * | 2005-12-19 | 2007-01-31 | 祖述冲 | Cell multiple-grooved incubator |
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| CN102807952A (en) | 2012-12-05 |
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