CN112899161A

CN112899161A - Cell culture device

Info

Publication number: CN112899161A
Application number: CN202110161185.4A
Authority: CN
Inventors: 费小涛
Original assignee: Shaoxing Gaoyanzhi Biotechnology Co ltd
Current assignee: Shaoxing Gaoyanzhi Biotechnology Co ltd
Priority date: 2021-02-05
Filing date: 2021-02-05
Publication date: 2021-06-04

Abstract

The invention discloses a cell culture device, which comprises a cell culture device body consisting of an incubator, an oxygen storage tank, an observation mechanism and a debugging mechanism, wherein a culture cavity is formed in the incubator, the bottom of the inner wall of the culture cavity is fixedly connected with a heating mechanism, the top of the heating mechanism is communicated with a water storage tank, the top of the water storage tank is sequentially provided with an air injection plate and a culture frame, and the outer side of the air injection plate is fixedly connected with the inner wall of the culture cavity. This cell culture device has solved prior art, and cell culture device is open cultivation for the majority, and the laboratory technician observes and need open the device and observe, causes very big pollution to inside, cultivates success rate greatly reduced, and few part direct observation can't adjust specifically observe every position, causes to observe the record inaccuracy, and the temperature of culture dish can not obtain fine constant temperature simultaneously, and the internal heating still causes bacterial contamination's problem easily.

Description

Cell culture device

Technical Field

The invention relates to the technical field of biology, in particular to a cell culture device.

Background

Cell culture refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain main structure and function, and is also called cell cloning technology, the formal term in biology is cell culture technology, and cell culture is an essential process for the whole bioengineering technology and one of the biological cloning technologies, the cell culture is large-scale cloning of cells, the cell culture technology can be used for making a large amount of cells into simple single cells or rarely differentiated multiple cells through mass culture, which is an essential link of the cloning technology, and the cell culture is cloning of the cells. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.

According to the Chinese patent CN108546644A, the invention discloses a cell culture device for biotechnology, which comprises a base, wherein the outer wall of the top of the base is connected with a box body through bolts, the inner wall of one side, opposite to the box body, of the box body is connected with connecting rods through bolts, the outer wall of one side, opposite to the two connecting rods, of the box body is connected with the same heating wire through bolts, the inner wall of one side, opposite to the box body, of the box body is connected with the same water storage box through bolts, purified water is filled in the water storage box, the inner wall of one side, opposite to the box body, of the. The invention has good growth environment of cells, accelerates the growth speed of the cells, shortens the cell culture period, accelerates the efficiency of biotechnological research work, increases the temperature of purified water in the water storage tank by the heating wire, thereby increasing the temperature in the culture bottle, avoiding the damage of the cultured cells caused by high temperature, and providing good growth environment for the cells in the culture bottle.

Among the prior art, cell culture device is open cultivation for the majority, and the laboratory technician observes and need open the device and observe, causes very big pollution to inside, cultivates success rate greatly reduced, and few partial direct observation can't adjust specifically observe every position, causes and observes the record inaccuracy, and the temperature of culture dish can not obtain fine constant temperature simultaneously, and internal heating still causes bacterial contamination easily.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a cell culture device, which solves the problems that most of cell culture devices are open culture devices in the prior art, an experimenter needs to open the device for observation, great pollution is caused to the inside, the success rate of culture is greatly reduced, few parts can not be directly observed to adjust and specifically observe each part, the observation record is inaccurate, the temperature of a culture dish can not be well kept constant, and bacterial pollution is easily caused by internal heating.

(II) technical scheme

In order to achieve the purpose, the invention is realized by the following technical scheme: the utility model provides a cell culture device, includes the incubator, deposits the cell culture device body that oxygen jar, observation mechanism and debugging mechanism constitute, the cultivation chamber has been seted up to the inside of incubator, the inner wall bottom fixedly connected with heating mechanism who cultivates the chamber, the top intercommunication of heating mechanism has the water storage box, the top of water storage box has set gradually gas injection board and has cultivateed the frame, the outside of gas injection board and the inner wall fixed connection who cultivates the chamber, the inside of cultivateing the chamber is provided with the dwang, the outside of dwang and the inside fixed connection who cultivates the frame, the top of gas injection board is provided with jet-propelled mechanism, the inside fixedly connected with culture dish of cultivateing the frame.

Preferably, the inside of depositing the oxygen jar is linked together through the gas-supply pipe with the inside of cultivateing the chamber, heating mechanism's top extends to the inside of water storage box, heating mechanism is from taking the jet-propelled function, bleeder vent and exhaust hole have been seted up in proper order from the top down in the inside of incubator.

Compared with the prior art, the purified water in the water storage tank is heated at high temperature and killed by the heating mechanism, the heating mechanism has a blowing function, the high-temperature gas flows into the gas spraying plate, the gas spraying mechanism dissipates the heat of the high-temperature gas, so that the outer surface of the culture dish is uniformly heated by hot gas, the temperature of water is constantly controlled by the temperature controller in the water storage tank, the cells cultured by the high temperature are prevented from being damaged, a good growth environment is provided for the cells in the culture dish, the hot gas pressure in the culture cavity is controlled by the vent holes to keep a certain temperature and humidity, a good living environment is provided for the cells, the impurity gas generated in the cell growth process is discharged by the vent holes, the oxygen concentration in the culture cavity is controlled by the oxygen storage tank, the cell is more favorable for the survival of the cells, the position of the culture dish can be automatically adjusted by combining the culture rack and the rotating rod, the observation that is favorable to the laboratory technician, debugging mechanism can finely tune the culture dish simultaneously, and observation mechanism enlargies the inside of culture dish, and the accurate observation record of favourable and laboratory technician need not take out the culture dish and observes, has prevented that external bacterial impurity from getting into and causing the pollution to the culture dish, has improved the survival rate of cell.

Preferably, cultivate the frame including cultivateing the dish, the inside sliding connection who cultivates the dish cultivates the extension board, cultivate the inside and culture dish sliding connection of extension board, cultivate the outside fixedly connected with magnetic strip of extension board, cultivate the outside of dish and cultivate the inner wall fixed connection in chamber, cultivate the inside and dwang fixed connection of dish.

Preferably, observation mechanism is including observing the frame, the outside of observing the frame and the inside fixed connection of incubator, the bottom of observing the frame runs through the incubator and extends to the inside of incubator, the inside from the top down of observing the frame has set gradually concave lens and convex lens, the right side threaded connection threaded rod of concave lens, the bottom of threaded rod is connected with the inside rotation of observing the frame, the outside of convex lens and the inside fixed connection of observing the frame.

Preferably, the debugging mechanism includes the debugging piece, the outside of debugging piece and the inside sliding connection of incubator, the inside sliding connection of debugging piece has the debugging pole, the left end fixedly connected with magnetic block of debugging pole, the right side fixedly connected with of magnetic block resumes the spring, the right-hand member of resuming the spring and the inside fixed connection of debugging piece.

Preferably, the left side of the magnetic block is magnetically connected with the right side of the magnetic strip.

Compare with the tradition, through the dwang, it makes things convenient for selection of experimenter to drive the culture rack rotation, can observe the inside cell of every culture dish, prevent external environment's pollution, magnetic block magnetic force acts on the culture rack magnetic force strip in the debugging mechanism simultaneously, it carries out accurate correction to the culture dish position to have made things convenient for the experimenter, concave lens can adjust the dwang and adjust from top to bottom on the observation mechanism, application Galileo telescope principle, enlarge the observation to culture dish inside, it is clearer to observe, the observation has prevented simultaneously to take out to observe and has wholly caused the pollution to the culture chamber, observation mechanism and debugging mechanism two combine to make things convenient for selection of experimenter to observe simultaneously and need not take out, time saving, can not cause any pollution to culture chamber and culture dish.

Preferably, the air injection mechanism comprises an air injection block, the air injection block is internally provided with an air delivery pipe, the top of the air injection block is fixedly connected with an air injection valve, the top end of the air delivery pipe is communicated with the air injection valve, the bottom of the air delivery pipe is communicated with an air delivery ring block, and the bottom of the air injection block is provided with a buffer groove.

Preferably, the receiving opening is formed in the receiving strip and communicated with the bottom of the gas conveying pipe, and a sterilization mechanism is fixedly connected to the inner wall of the receiving opening.

Preferably, the sterilization mechanism comprises a middle fixed frame, a microwave device is arranged inside the middle fixed frame, a flow guide opening is formed inside the middle fixed frame, a water outlet hole is formed in the bottom of the middle fixed frame, a sleeve is communicated with the top of the middle fixed frame, and the top end of the sleeve is communicated with the bearing opening.

Compared with the prior art, the microwave device in the sterilization mechanism utilizes electromagnetic waves to drive polar molecules in high-temperature gas to rotate, the mutual friction generates high temperature, the sterilization of the high-temperature gas in the middle and the solid frames is carried out, meanwhile, the outside gas of the water storage tank is sterilized, the sterile culture environment of the culture chamber is provided, the growth of cells in the culture dish is more facilitated, water drops after redundant condensation can flow into the water outlet holes, the contact area between the carrying strip and the middle and solid frames and the high-temperature gas is increased, the rapid temperature rise of the culture dish in a short time is facilitated, meanwhile, the utilization rate of the high-temperature gas is improved, the gas conveying pipe can filter large water drops in the high-temperature gas, the temperature and the humidity in the culture chamber are ensured, the slow flow groove is beneficial for the water drops condensed in the gas conveying pipe to flow out of the gas injection mechanism again, the water flows into the water storage, simultaneously, each culture dish and each part are guaranteed to be heated evenly, and the culture of cells in the culture dish is more facilitated.

(III) advantageous effects

The invention provides a cell culture device. The method has the following beneficial effects:

(1) the culture dish is provided with the heating mechanism and the air injection mechanism, purified water in the water storage box is heated and killed at high temperature through the heating mechanism, meanwhile, the heating mechanism is provided with an air injection function, high-temperature gas flows into the air injection plate, the air injection mechanism conducts heat dissipation on the high-temperature gas, the outer surface of the culture dish is uniformly heated by the hot gas, the temperature controller is arranged in the water storage box to constantly control the temperature of the water, the cultured cells are prevented from being damaged by the high temperature, and a good growth environment is provided for the cells in the culture dish.

(2) The invention is provided with the air holes and the exhaust holes, the hot air pressure in the culture cavity is controlled by the air holes to keep a certain temperature and humidity, a good living environment is improved for cells, the exhaust holes remove impurity gas generated in the cell growth process, and the oxygen concentration in the culture cavity is controlled by matching with the oxygen storage tank, so that the cell survival is facilitated.

(3) The culture dish position adjusting device is provided with the debugging mechanism and the observing mechanism, the position of the culture dish can be automatically adjusted through the combination of the culture rack and the rotating rod, any culture dish can be observed, the observation of experimenters is facilitated, meanwhile, the debugging mechanism can finely adjust the culture dish, the observing mechanism amplifies the interior of the culture dish, accurate observation records of the experimenters are facilitated, the culture dish does not need to be taken out for observation, external bacterial impurities are prevented from entering the culture dish to cause pollution, and the survival rate of cells is improved.

(4) The culture rack is provided with the debugging mechanism, the culture rack is driven to rotate through the rotating rod, so that the selection of experimenters is facilitated, the internal cells of each culture dish can be observed, the pollution of the external environment is prevented, and meanwhile, the magnetic force of the magnetic block in the debugging mechanism acts on the magnetic force strip on the culture rack, so that the experimenters can conveniently and accurately correct the positions of the culture dishes.

(5) The invention is provided with an observation mechanism, a rotating rod can be adjusted up and down through a concave lens on the observation mechanism, the principle of Galileo telescope is applied, in order to obtain upright cells in a culture dish, the objective lens uses a convex lens with longer focal length, the eyepiece uses a concave lens, light rays emitted by a distant object meet the eyepiece (concave lens) after passing through the objective lens before converging and imaging, the converging light rays are diverged, and the intersection point of reverse extension lines of the diverging light rays forms an upright virtual image so as to observe cell growth, thereby being more beneficial to observing the cells and culture solution in the culture dish and preventing the whole culture cavity from being polluted by taking out and observing.

(6) The invention is provided with a sterilization mechanism, and by using an electromagnetic wave with the microwave frequency between MHz through a microwave device in the sterilization mechanism, water can strongly absorb microwaves, so that polar molecules of the water rotate, the molecules generate heat by friction, the temperature rises rapidly, the water can be sterilized by heat, the temperature can reach to DEG C within tens of seconds to a few minutes, and microorganisms in liquid can be completely killed, so that the water sterilization device is suitable for sterilizing water, sterilizing high-temperature gas in the middle-fixed frame, sterilizing the gas outside the water storage tank, providing a sterile culture environment for a culture cavity, and being more beneficial to the growth of cells in the culture dish.

(7) The culture dish heating device is provided with the bearing strips and the middle fixing frames, and the contact area between the bearing strips and the middle fixing frames and the high-temperature gas is increased, so that the culture dish can be heated quickly in a short time, the utilization rate of the high-temperature gas is improved, and redundant condensed water drops can flow into the water outlet holes.

(8) The culture cavity is provided with the gas conveying pipe and the slow flow groove, large water drops in high-temperature gas can be filtered through the gas conveying pipe, the temperature and the humidity in the culture cavity are guaranteed, and the slow flow groove is beneficial to enabling the condensed water drops in the gas conveying pipe to flow out of the gas injection mechanism again and flow into the water storage tank.

(9) The invention is provided with the air injection valve, the diffusion area of high-temperature gas is increased through the air injection valve, the culture dish is favorable for rapid heating, each culture dish and each part are ensured to be uniformly heated, and the culture of cells in the culture dish is more favorable.

Drawings

FIG. 1 is a schematic structural diagram of a novel solid of the present invention;

FIG. 2 is a schematic structural view of a novel cross-section of the present invention;

FIG. 3 is a schematic three-dimensional structure of the novel cultivation shelf of the present invention;

FIG. 4 is a schematic structural diagram of a section of the novel observation mechanism of the present invention;

FIG. 5 is a schematic structural diagram of a section of the novel debugging mechanism of the invention;

FIG. 6 is a schematic structural view of a section of the novel air injection mechanism of the present invention;

FIG. 7 is a schematic structural view of a section of the novel gas delivery ring block of the present invention;

fig. 8 is a schematic structural view of a section of the novel sterilization mechanism of the present invention.

In the figure: 1 cell culture device body, 2 culture box, 21 culture cavity, 211 air vent, 212 air vent, 22 heating mechanism, 23 water storage tank, 24 air jet plate, 25 culture shelf, 251 culture tray, 252 culture support plate, 253 magnetic strip, 26 rotating rod, 27 air jet mechanism, 271 air jet block, 272 air pipe, 273 air jet valve, 274 air delivery ring block, 2741 receiving strip, 2742 receiving port, 2743 sterilization mechanism, 27431 middle fixing frame, 27432 microwave device, 27433 diversion port, 27434 water outlet hole, 27435 sleeve, 275 slow flow groove, 28 culture dish, 3 oxygen storage tank, 4 observation mechanism, 41 observation frame, 42 concave lens, 43 convex lens, 44 threaded rod, 5 debugging mechanism, 51 debugging block, 52 debugging rod, 53 block, 54 magnetic force recovery spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The first embodiment is as follows:

referring to fig. 1-2, the present invention provides a technical solution: the utility model provides a cell culture device, including incubator 2, deposit oxygen jar 3, the cell culture device body 1 that observation mechanism 4 and debugging mechanism 5 are constituteed, cultivate chamber 21 has been seted up to incubator 2's inside, the inner wall bottom fixedly connected with heating mechanism 22 of cultivating chamber 21, the top intercommunication of heating mechanism 22 has water storage box 23, the top of water storage box 23 has set gradually jet-propelled board 24 and has cultivateed frame 25, the outside of jet-propelled board 24 and the inner wall fixed connection who cultivates chamber 21, the inside of cultivateing chamber 21 is provided with dwang 26, the outside of dwang 26 and the inside fixed connection of cultivateing frame 25, the top of jet-propelled board 24 is provided with jet-propelled mechanism 27, the inside fixedly connected with culture dish 28 of cultivateing frame 25.

The inside of oxygen storage tank 3 is communicated with the inside of culture cavity 21 through a gas pipe, the top of heating mechanism 22 extends to the inside of water storage tank 23, heating mechanism 22 has a gas injection function, and vent holes 211 and exhaust holes 212 are sequentially formed in the inside of incubator 2 from top to bottom.

When the device is used, an experimenter opens the debugging mechanism 5, the prepared culture dishes 28 are placed on the culture frame 25 one by one, the external controller controls oxygen in the oxygen storage tank 3 to flow into the culture cavity 21, the external power supply is switched on, the heating mechanism 22 heats purified water inside the water storage tank 23, the heating mechanism 22 has a blowing function, high-temperature gas is guided into the air injection mechanism 27 through the air injection plate 24, the air injection mechanism 27 uniformly heats the culture dishes 28, redundant hot gas is discharged through the air holes 211, waste gas generated by the culture dishes 28 is discharged through the exhaust holes 212, when observation is needed, the rotating rod 26 is driven by the external motor to drive the culture frame 25 to rotate and debug to a proper position, the debugging mechanism 5 is adjusted to perform accurate positioning, observation is performed through the observation mechanism 4, compared with the prior art, the purified water inside the water storage tank 23 is heated and killed at high temperature through the heating mechanism 22, meanwhile, the heating mechanism 22 has a blowing function, high-temperature gas flows into the air injection plate 24, the air injection mechanism 27 radiates heat to the high-temperature gas, so that the outer surface of the culture dish 28 is uniformly heated by hot gas, a temperature controller is arranged in the water storage tank 23 to constantly control the temperature of water, the cultured cells are prevented from being damaged by the high temperature, a good growth environment is provided for the cells in the culture dish 28, the air holes 211 control the hot air pressure in the culture cavity 21 to keep a certain temperature and humidity, so that a good survival environment is provided for the cells, the air holes 212 exhaust impurity gas generated in the cell growth process, the oxygen concentration in the culture cavity 21 is controlled by matching with the oxygen storage tank 3, the existence of the cells is facilitated, the position of the culture dish 28 can be automatically adjusted by combining the culture rack 25 and the rotating rod 26, any culture dish 28 can be observed, and the observation of a laboratory technician is facilitated, simultaneously debugging mechanism 5 can finely tune culture dish 28, and observation mechanism 4 enlargies the inside of culture dish 28, favourable and the accurate observation record of laboratory technician more need not take out culture dish 28 and observe, has prevented that external bacterial impurity from getting into to cause the pollution to culture dish 28, has improved the survival rate of cell.

Example two:

referring to fig. 1-5, on the basis of the first embodiment, the present invention provides a technical solution: the culture shelf 25 comprises a culture tray 251, a culture strip 252 is connected to the interior of the culture tray 251 in a sliding manner, the interior of the culture strip 252 is connected to the culture dish 28 in a sliding manner, a magnetic strip 253 is fixedly connected to the outer side of the culture strip 252, the outer side of the culture tray 251 is fixedly connected to the inner wall of the culture cavity 21, and the interior of the culture tray 251 is fixedly connected to the rotating rod 26.

The observation mechanism 4 comprises an observation frame 41, the outer side of the observation frame 41 is fixedly connected with the inside of the incubator 2, the bottom of the observation frame 41 penetrates through the incubator 2 and extends into the incubator 2, a concave lens 42 and a convex lens 43 are sequentially arranged in the observation frame 41 from top to bottom, a threaded rod 44 is connected with the right side of the concave lens 42 in a threaded manner, the bottom of the threaded rod 44 is rotatably connected with the inside of the observation frame 41, and the outer side of the convex lens 43 is fixedly connected with the inside of the observation frame 41.

The debugging mechanism 5 comprises a debugging block 51, the outer side of the debugging block 51 is connected with the inner sliding part of the incubator 2, the inner sliding part of the debugging block 51 is connected with a debugging rod 52, the left end of the debugging rod 52 is fixedly connected with a magnetic block 53, the right side of the magnetic block 53 is fixedly connected with a restoring spring 54, and the right end of the restoring spring 54 is fixedly connected with the inner part of the debugging block 51.

The left side of the magnet block 53 is magnetically coupled to the right side of the magnet bar 253.

When in use, when observation is needed, the rotating rod 26 is driven by an external motor to drive the culture rack 25 to rotate, the culture dish 28 is selected to be roughly positioned, then an experimenter pushes the debugging rod 52 in the debugging mechanism 5 to drive the restoring spring 54 to stretch, the magnetic block 53 is driven to move forwards, the magnetic block 53 and the magnetic strip 253 are mutually acted by magnetic force to adjust the debugging rod 52, the culture dish 28 on the culture rack 25 is driven to slightly move, the threaded rod 44 on the observation mechanism 4 is simultaneously adjusted to drive the concave lens 42 to finely adjust up and down, the culture dish 28 is observed, compared with the prior art, the culture rack 25 is driven to rotate by the rotating rod 26, the experimenter can conveniently select, the internal cells of each culture dish 28 can be observed, the pollution of the external environment is prevented, meanwhile, the magnetic block 53 in the debugging mechanism 5 acts on the magnetic strip 253 on the culture rack 25 by magnetic force, and the experimenter can conveniently and accurately correct the position of the culture dish 28, the concave lens 42 on the observation mechanism 4 can adjust the dwang 26 and adjust from top to bottom, and the application Galileo telescope principle is used to enlarge the observation inside the culture dish 28, and the observation is clearer, and the observation has been prevented simultaneously and has been observed and to have led to the fact the pollution to culture chamber 21 is whole, and observation mechanism 4 and the two of debugging mechanism 5 combine to make things convenient for the selection of experimenter to observe and need not take out simultaneously, saves time, can not cause any pollution to culture chamber 21 and culture dish 28.

Example three:

referring to fig. 1-8, on the basis of the first embodiment and the second embodiment, the present invention provides a technical solution: the air injection mechanism 27 comprises an air injection block 271, an air pipe 272 is arranged inside the air injection block 271, an air injection valve 273 is fixedly connected to the top of the air injection block 271, the top end of the air pipe 272 is communicated with the air injection valve 273, an air conveying ring block 274 is communicated with the bottom of the air pipe 272, and a buffer groove 275 is formed in the bottom of the air injection block 271.

The air delivery ring block 274 comprises a receiving strip 2741, the outer side of the receiving strip 2741 is fixedly connected with the inside of the air injection block 271, a receiving port 2742 is formed in the receiving strip 2741, the receiving port 2742 is communicated with the bottom of the air delivery pipe 272, and a sterilization mechanism 2743 is fixedly connected to the inner wall of the receiving port 2742.

The sterilization mechanism 2743 comprises a middle fixing frame 27431, a microwave 27432 is arranged inside the middle fixing frame 27431, a flow guide port 27433 is formed inside the middle fixing frame 27431, a water outlet hole 27434 is formed in the bottom of the middle fixing frame 27431, a sleeve 27435 is communicated with the top of the middle fixing frame 27431, and the top end of the sleeve 27435 is communicated with the bearing port 2742.

When in use, the external power is switched on, the heating mechanism 22 heats the purified water inside the water storage tank 23, the heating mechanism 22 has a blowing function, high-temperature gas is introduced into the blowing mechanism 27 through the blowing plate 24, the high-temperature gas enters through the flow guide port 27433, the microwave 27432 facilitates the ultrasonic wave to drive the polar molecules in the high-temperature gas to rotate, the mutual friction generates high temperature for sterilization, the high-temperature gas flows into the sleeve pipe 27435, flows into the receiving port 2742, flows into the gas pipe 272 and flows into the gas spraying valve 273 for emission, compared with the prior art, the microwave 27432 in the sterilization mechanism 2743 drives the polar molecules in the high-temperature gas to rotate by using electromagnetic wave, the mutual friction generates high temperature, the sterilization is performed on the high-temperature gas inside the fixing frame 27431, meanwhile, the external gas of the water storage tank 23 is sterilized, the sterile culture environment of the culture chamber 21 is provided, and the growth, apopore 27434 can flow in the water droplet after the unnecessary condensation, accept strip 2741 and well solid frame 27431 through increase and high-temperature gas's area of contact, be favorable to carrying out rapid heating up to culture dish 28 in short time, simultaneously, high-temperature gas's utilization ratio improves, gas-supply pipe 272 can filter great drop of water in the high-temperature gas, guarantee the inside temperature and the humidity in culture chamber 21, slow groove 275 is favorable to the drop of water of condensation among the gas-supply pipe 272 to flow out air jet mechanism 27 again, flow in the water storage tank 23, air jet valve 273 has increased high-temperature gas's the area of dispersing, be favorable to the rapid heating up of culture dish 28, guarantee every culture dish 28 and each position thermally equivalent simultaneously, be favorable to the cultivation of cell in culture dish 28 more.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. The term "comprising", without further limitation, means that the element so defined is not excluded from the group consisting of additional identical elements in the process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a cell culture device, includes incubator (2), deposits oxygen jar (3), observation mechanism (4) and debugging mechanism (5) and constitutes cell culture device body (1), its characterized in that: cultivate chamber (21) has been seted up to the inside of incubator (2), the inner wall bottom fixedly connected with heating mechanism (22) of cultivateing chamber (21), the top intercommunication of heating mechanism (22) has water storage box (23), the top of water storage box (23) has set gradually jet plate (24) and has cultivateed frame (25), the outside of jet plate (24) and the inner wall fixed connection who cultivates chamber (21), the inside of cultivateing chamber (21) is provided with dwang (26), the outside of dwang (26) and the inside fixed connection who cultivates frame (25), the top of jet plate (24) is provided with jet mechanism (27), the inside fixedly connected with culture dish (28) of cultivateing frame (25).

2. A cell culture apparatus according to claim 1, wherein: deposit the inside of oxygen jar (3) and be linked together through the inside of gas-supply pipe with cultivate chamber (21), the top of heating mechanism (22) extends to the inside of water storage box (23), heating mechanism (22) is from taking air injection function, bleeder vent (211) and exhaust hole (212) have been seted up in proper order from the top down to the inside of incubator (2).

3. A cell culture apparatus according to claim 1, wherein: the culture rack (25) comprises a culture tray (251), a culture plate (252) is connected to the interior of the culture tray (251) in a sliding mode, the interior of the culture plate (252) is connected with a culture dish (28) in a sliding mode, a magnetic strip (253) is fixedly connected to the outer side of the culture plate (252), the outer side of the culture tray (251) is fixedly connected with the inner wall of a culture cavity (21), and the interior of the culture tray (251) is fixedly connected with a rotating rod (26).

4. A cell culture apparatus according to claim 1, wherein: observation mechanism (4) are including observing frame (41), the outside of observing frame (41) and the inside fixed connection of incubator (2), the inside that incubator (2) were run through and extend to incubator (2) is run through to the bottom of observing frame (41), the inside from the top down of observing frame (41) has set gradually concave lens (42) and convex lens (43), the right side threaded connection threaded rod (44) of concave lens (42), the bottom of threaded rod (44) is connected with the inside rotation of observing frame (41), the outside of convex lens (43) and the inside fixed connection of observing frame (41).

5. A cell culture apparatus according to claim 1, wherein: debugging mechanism (5) are including debugging piece (51), the outside of debugging piece (51) and the inside sliding connection of incubator (2), the inside sliding connection of debugging piece (51) has debugging pole (52), the left end fixedly connected with magnetic block (53) of debugging pole (52), the right side fixedly connected with of magnetic block (53) resumes spring (54), the right-hand member of resuming spring (54) and the inside fixed connection of debugging piece (51).

6. A cell culture assembly according to claim 5 wherein: the left side of the magnetic block (53) is magnetically connected with the right side of the magnetic strip (253).

7. A cell culture apparatus according to claim 1, wherein: the air injection mechanism (27) comprises an air injection block (271), an air injection pipe (272) is arranged inside the air injection block (271), an air injection valve (273) is fixedly connected to the top of the air injection block (271), the top end of the air injection pipe (272) is communicated with the air injection valve (273), the bottom of the air injection pipe (272) is communicated with an air injection ring block (274), and a flow buffering groove (275) is formed in the bottom of the air injection block (271).

8. A cell culture assembly according to claim 7 wherein: the gas transmission ring block (274) comprises a bearing strip (2741), the outer side of the bearing strip (2741) is fixedly connected with the inside of the gas injection block (271), a bearing port (2742) is formed in the bearing strip (2741), the bearing port (2742) is communicated with the bottom of the gas transmission pipe (272), and a sterilization mechanism (2743) is fixedly connected to the inner wall of the bearing port (2742).

9. A cell culture apparatus according to claim 1, wherein: the sterilization mechanism (2743) comprises a middle fixing frame (27431), a microwave device (27432) is arranged inside the middle fixing frame (27431), a flow guide port (27433) is formed inside the middle fixing frame (27431), a water outlet hole (27434) is formed in the bottom of the middle fixing frame (27431), the top of the middle fixing frame (27431) is communicated with a sleeve (27435), and the top end of the sleeve (27435) is communicated with a bearing port (2742).