CN112226366A - Low oxygen cell culture device - Google Patents
Low oxygen cell culture device Download PDFInfo
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- CN112226366A CN112226366A CN202011129656.5A CN202011129656A CN112226366A CN 112226366 A CN112226366 A CN 112226366A CN 202011129656 A CN202011129656 A CN 202011129656A CN 112226366 A CN112226366 A CN 112226366A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 53
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title description 13
- 239000001301 oxygen Substances 0.000 title description 13
- 229910052760 oxygen Inorganic materials 0.000 title description 13
- 239000011521 glass Substances 0.000 claims abstract description 68
- 206010021143 Hypoxia Diseases 0.000 claims abstract description 25
- 230000001146 hypoxic effect Effects 0.000 claims abstract description 23
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 12
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 9
- 239000001569 carbon dioxide Substances 0.000 claims description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 239000007789 gas Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 230000008859 change Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007954 hypoxia Effects 0.000 description 2
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
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Abstract
The invention belongs to the technical field of hypoxic cell culture, in particular to a hypoxic cell culture device, which comprises a control center and a culture chamber, two sides of the outer surface of the control center are fixedly connected with a second fixing block through screws, a first support rod is arranged above the culture chamber, a plurality of first fixing blocks are bonded on both sides of the upper surface of the culture chamber, adjusting bolts are screwed and connected on one side inside the second fixing block, a plurality of grooves are respectively arranged in the first transparent glass plate and the second transparent glass plate, so that a culture dish for cell culture can be conveniently placed in the first transparent glass plate and the second transparent glass plate, meanwhile, the two sides of the groove of the first transparent glass plate and the second transparent glass plate are respectively bonded with a limiting spring group, and through the limiting of the culture dishes of the limiting spring groups at the two sides, thereby the stability that the culture dish was placed in No. one transparent glass board and No. two transparent glass boards has been fine guaranteed.
Description
Technical Field
The invention belongs to the technical field of hypoxic cell culture, and particularly relates to a hypoxic cell culture device.
Background
Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.
The hypoxic cell culture is an important one in a cell culture mode, usually, a certain auxiliary device is needed to complete the culture during the hypoxic cell culture, the existing hypoxic cell culture chamber of the MAWORDE star product can well simulate the living environment of cells in an organism to culture the cells, however, the device has a certain limitation in the using process, namely a culture dish for placing cell sap, when the device is placed inside for culture, the workers can only observe the state change of the cell culture above the culture dish, but cannot well observe the cell culture below the culture dish, thereby the contrast of experimenter below can not carry out for can not in time carry out the concentration of more accurate adjusting device interior oxygen, make cell culture's effect better, this device still lacks certain practicality simultaneously.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the hypoxic cell culture device which has the characteristics that experimenters can observe the upper part and the lower part of the cell culture state in the device in time conveniently, the oxygen concentration in the control device can be adjusted accurately in time, the experimental result is more accurate, and meanwhile, the device has better practicability.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a hypoxia cell culture device, includes control center and cultivates the room, the fixed block No. two of screw fixedly connected with is all passed through to control center surface both sides, it is provided with the bracing piece No. one to cultivate the room top, it all bonds a plurality of fixed block to cultivate room upper surface both sides, the inside one side of No. two fixed blocks closes soon and is connected with adjusting bolt, adjusting bolt surface one side end is rotated through the bearing and is connected with the bracing piece No. two, it bonds No. one transparent glass board to cultivate indoor portion one side, and has seted up a plurality of recess on one side of a transparent glass inboard portion, the inside bonding of a transparent glass inboard has the spacing spring assembly of a plurality of, a transparent glass board top is provided with No. two transparent glass boards.
According to the preferred technical scheme of the hypoxic cell culture device, the control rod is rotatably connected above the inner part of the culture chamber, the third fixing block is bonded to the tail end of one side of the outer surface of the control rod, the second transparent glass plate is bonded to two sides of the outer surface of the third fixing block, and an observation notch is formed in one side of the inner part of the first transparent glass plate.
As a preferred technical scheme of the hypoxic cell culture device, four fixing blocks are bonded on two sides of the upper surface of the control center, and a limiting block is connected to one side of the outer surface of each fixing block in a clamping mode.
In a preferred embodiment of the hypoxic cell culture apparatus of the present invention, the sealing door is rotatably connected to one side of the inside of the culture chamber by a plurality of hinges.
As a preferred technical scheme of the hypoxic cell culture device, a handle rod is welded on one side of the outer surface of the first fixing block.
In a preferred embodiment of the hypoxic cell culture device, the end of the second support rod on one side of the outer surface is connected to the inside of the first support rod in a clamping manner.
As a preferred technical scheme of the hypoxic cell culture device, the adjusting bolt and the tail end of one side of the outer surface of the control rod are respectively provided with a rotating handle
As a preferred technical scheme of the hypoxic cell culture device, the flexible pipe is connected with a nitrogen cylinder and a carbon dioxide cylinder respectively inside the culture chamber.
Compared with the prior art, the invention has the beneficial effects that: the culture dishes for cell culture are placed inside the first transparent glass plate and the second transparent glass plate, the limiting spring groups are bonded on two sides of the grooves of the first transparent glass plate and the second transparent glass plate, and the culture dishes of different sizes can be stably placed by limiting the limiting spring groups on two sides of the culture dishes, so that the device is more practical; by rotating the adjusting bolt on one side inside the second fixing block, the second supporting rod pushes the first supporting rod to move upwards, so that the culture room is kept stable and moves upwards to a proper position, and an experimenter can observe the cell culture state below a culture dish placed inside the culture room conveniently; one side of the upper surface of the culture chamber is connected with the first supporting rod in a clamping manner through a plurality of first fixing blocks, and the connecting part is fixed through bolts, so that the first supporting rod is convenient to mount, fix and dismount; no. four fixed block surface one side blocks are connected with the stopper, and the block junction is by the bolt fastening for the stopper can carry on spacingly to cultivateing the room, thereby has guaranteed to cultivate the room and has placed the stability when control center upper surface, can adjust the position of stopper through the mode of not hard up bolt simultaneously, thereby reaches to cultivate the room homoenergetic to different dimensions and carry out suitable fixed, has further increased the practicality of this device.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view showing the structure of the inside of the culture chamber according to the present invention in detail;
FIG. 3 is a schematic structural view of a detailed connection section of a second support rod and an adjusting bolt in the invention;
FIG. 4 is a schematic view showing the detailed connection structure between the first support rod and the second support rod according to the present invention;
FIG. 5 is an enlarged schematic view of the connection between the first transparent glass plate and the limiting spring set;
FIG. 6 is a schematic structural diagram of the fourth fixing block and a limiting block which are connected in detail and enlarged;
FIG. 7 is a schematic view showing the structure of the culture chamber in detail connected with a nitrogen gas cylinder and a carbon dioxide gas cylinder according to the present invention;
in the figure: 1. a control center; 2. a sealing door; 3. a culture chamber; 4. a first fixed block; 5. a handle bar; 6. a first support rod; 7. a second support rod; 8. a second fixed block; 9. adjusting the bolt; 10. a first transparent glass plate; 11. a limiting spring set; 12. a third fixed block; 13. a control lever; 14. a second transparent glass plate; 15. an observation notch; 16. a bearing; 17. a fourth fixed block; 18. a limiting block; 19. a nitrogen gas cylinder; 20. a carbon dioxide cylinder.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
Referring to fig. 1-7, the present invention provides the following technical solutions: a hypoxia cell culture device comprises a control center 1 and a culture chamber 3, wherein two sides of the outer surface of the control center 1 are fixedly connected with a second fixing block 8 through screws, a first support rod 6 is arranged above the culture chamber 3, a plurality of first fixing blocks 4 are adhered to two sides of the upper surface of the culture chamber 3, one side inside the second fixing block 8 is screwed and connected with an adjusting bolt 9, the tail end of one side of the outer surface of the adjusting bolt 9 is rotatably connected with a second support rod 7 through a bearing 16, a first transparent glass plate 10 is adhered to one side inside the culture chamber 3, a plurality of grooves are formed in one side inside the first transparent glass plate 10, a plurality of limiting spring groups 11 are adhered to the inside of the first transparent glass plate 10, a second transparent glass plate 14 is arranged above the first transparent glass plate 10, in the embodiment, a lead and a pipeline are connected between the control center 1 and the culture chamber 3, the device is convenient for controlling the change of the oxygen concentration inside the culture chamber 3 in real time through the control center 1, meanwhile, a first transparent glass plate 10 and a second transparent glass plate 14 are respectively arranged inside the culture chamber 3, a plurality of grooves are respectively arranged inside the first transparent glass plate 10 and the second transparent glass plate 14, culture dishes for cell culture are conveniently placed inside the first transparent glass plate 10 and the second transparent glass plate 14 through the grooves, meanwhile, the limiting spring groups 11 are respectively bonded on two sides of the grooves of the first transparent glass plate 10 and the second transparent glass plate 14, and the culture dishes of different models and sizes can be conveniently cultured by the extrusion of the limiting spring groups 11 on two sides, so that the stability of the culture dishes placed inside the first transparent glass plate 10 and the second transparent glass plate 14 is well ensured, meanwhile, because of the deformation of the limiting spring groups 11, the stability can be ensured, so that the practicability of the device is better; when needing to observe the lower part of the cell culture state in the culture room 3, the adjusting bolt 9 on one side in the second fixing block 8 rotates, and the tail end on one side of the outer surface of the adjusting bolt 9 is connected with the second supporting rod 7 through the bearing 16, so that the fixed position of the second supporting rod 7 cannot be influenced when the adjusting bolt 9 rotates, the second supporting rod 7 pushes the first supporting rod 6 to move upwards, the culture room 3 is kept stable and moves upwards to a proper position, and the laboratory staff can observe the cell culture state below a culture dish placed in the culture room 3 conveniently, so that the laboratory staff can observe the upper part and the lower part of the cell culture state in the device conveniently and timely, the oxygen concentration in the device can be accurately adjusted in time, and the experimental result is more accurate; simultaneously, one side of the upper surface of the culture chamber 3 is clamped and connected with a first supporting rod 6 through a plurality of first fixing blocks 4, and the joint is fixed through bolts, so that the first supporting rod 6 is convenient to mount, fix and dismount, meanwhile, a second fixing block 8 is fixedly connected with the control center 1 through screws, and the second fixing block 8 and the connecting part of the second fixing block 8 are also convenient to mount and dismount.
Specifically, a control rod 13 is rotatably connected above the inner part of the culture chamber 3, a third fixing block 12 is bonded at the tail end of one side of the outer surface of the control rod 13, a second transparent glass plate 14 is bonded at both sides of the outer surface of the third fixing block 12, and an observation notch 15 is formed at one side of the inner part of the first transparent glass plate 10, in the embodiment, the control rod 13 is rotatably connected above the inner part of the culture chamber 3, the third fixing block 12 is bonded at the tail end of the outer surface of the control rod 13, the support of the second transparent glass plates 14 at both sides is conveniently ensured through the third fixing block 12, the third fixing block 12 can drive the second transparent glass plate 14 to rotate together through the rotation of the control rod 13, meanwhile, the observation notch 15 is formed at one side of the inner part of the first transparent glass plate 10, the culture dish fixed inside the second transparent glass plate 14 can rotate to the position of the observation notch, the experimenter can observe the inside fixed culture dish below of No. two transparent glass plate 14 through observing notch 15, has guaranteed the experimenter and has observed the convenience of No. two transparent glass plate 14 culture dish below.
Specifically, four fixing blocks 17 are bonded on two sides of the upper surface of the control center 1, and a limiting block 18 is connected to one side of the outer surface of each four fixing block 17 in a clamping manner, in the embodiment, a plurality of four fixing blocks 17 are bonded on two sides of the upper surface of the control center 1, and a limiting block 18 is connected to one side of the outer surface of each four fixing block 17 in a clamping manner, and the clamping connection part is fixed by a bolt, so that the limiting block 18 can limit the culture chamber 3, the stability of the culture chamber 3 when placed on the upper surface of the control center 1 is ensured, and meanwhile, the position of the limiting block 18 can be adjusted in a bolt loosening manner, so that the culture chambers 3 with different sizes can be properly fixed, and the practicability of the device is further improved; simultaneously this device control center 1 is equipped with carbon dioxide, temperature, humidity, the detector of oxygen content to better real-time supervision each parameter value change, in time adjust when necessary, and control center 1 inside one side is furnished with the display simultaneously, carries out the data observation for the user.
It is specific, cultivate 3 inside one sides in room and be connected with sealing door 2 through a plurality of hinge rotation, in this embodiment, the notch has been seted up to 3 inside one sides in room of this device cultivation, be convenient for through the notch to the inside device of placing cell culture of cultivation room 3, simultaneously pass through hinge rotation connection sealing door 2 in notch position department, and sealing door 2 is connected with the notch block, thereby be convenient for cultivate 3 notch departments in room closed and open, sealing door 2 has the rubber pad with 3 notch block parcels in cultivation room, further guarantee sealing door 2 and the leakproofness of cultivating 3 notch block edges in room.
Specifically, 4 outside surface one side welding of fixed block has handle pole 5, in this embodiment, carries out the position transfer to cultivateing room 3 through handle pole 5 the laboratory technician of being convenient for.
Specifically, the terminal block in the surface of No. two bracing pieces 7 one side is connected inside bracing piece 6, and in this embodiment, the block in the surface of No. two bracing pieces 7 of this device one side is connected inside bracing piece 6, avoids No. two bracing pieces 7 to cause the slip when promoting a bracing piece 6 to move up, influences the stability that cultivates room 3 and shift up.
Specifically, adjusting bolt 9 and control lever 13 surface one side end all are provided with the rotation handle, and in this embodiment, through install the rotation handle at one side end of adjusting bolt 9 and control lever 13 to the experimenter of being convenient for more rotates adjusting bolt 9 and control lever 13
Specifically, cultivate the inside hose that leads to of room 3 and be connected with nitrogen cylinder 19 and carbon dioxide gas cylinder 20 respectively, in this embodiment, through connecting nitrogen cylinder 19 and carbon dioxide gas cylinder 20 to can fine control oxygen content, the gas pocket has been seted up to the inside one side of room 3 at the same time, and gas pocket department closes soon and is connected with the closing cap, so that oxygen get into in real time.
The working principle and the use of the invention are as follows: in the using process of the invention, the device needs to be sterilized before being used, so as to ensure the sterile environment of the device and be beneficial to cell culture, meanwhile, oxygen and carbon dioxide of the device are precisely regulated and controlled by an electronic valve, in addition, an incubator of the device is provided with three air holes, two of which are carbon dioxide and nitrogen, the remaining one is air hole for gas exchange and oxygen circulation, and three parts are provided with gas filtering devices, so as to ensure the sterile environment of a box body, a lead and a pipeline are connected between a control center 1 of the device and an incubation chamber 3, so that the change of the oxygen concentration in the incubation chamber 3 can be conveniently controlled in real time by the control center 1, meanwhile, a first transparent glass plate 10 and a second transparent glass plate 14 are respectively arranged in the incubation chamber 3 of the device, and a plurality of grooves are respectively arranged in the first transparent glass plate 10 and the second transparent glass plate 14, through the groove, the culture dish used for cell culture is conveniently placed inside the first transparent glass plate 10 and the second transparent glass plate 14, meanwhile, the limiting spring groups 11 are bonded on two sides of the groove of the first transparent glass plate 10 and the second transparent glass plate 14, and the culture dish is extruded by the limiting spring groups 11 on two sides, so that the stability of the culture dish placed inside the first transparent glass plate 10 and the second transparent glass plate 14 is well ensured, and meanwhile, due to the deformation of the limiting spring groups 11, the stability of the culture dishes of different models and sizes can be ensured, so that the practicability of the device is better; when needs are observed the below of 3 inside cell culture states in culture room, adjust bolt 9 through the inside one side to No. two fixed blocks 8 rotates, be connected with No. two bracing pieces 7 at adjust bolt 9 surface one side end simultaneously through bearing 16, thereby adjust bolt 9 can not influence the fixed position of No. two bracing pieces 7 when rotating, and can make No. two bracing pieces 7 push away bracing piece 6 and upwards move, and then reach and keep moving to suitable position on stabilizing culture room 3, thereby be convenient for the laboratory technician observe the cell culture state of the culture dish below of 3 inside places in culture room, through this mode, thereby be convenient for the timely upper and lower directions of observing the device interior cell culture state of laboratory staff, in time adjust accurate oxygen concentration to in the controlling means, make the experimental result more accurate.
Simultaneously in the inside top of culture room 3 rotation connection with control lever 13, and the terminal bonding of control lever 13 surface has No. three fixed blocks 12, be convenient for guarantee the support nature of No. two transparent glass boards 14 in both sides through No. three fixed blocks 12, through the rotation to control lever 13, make No. three fixed blocks 12 can drive No. two transparent glass boards 14 together and rotate, observation notch 15 has been seted up to one side in a transparent glass board 10 inside simultaneously, make No. two transparent glass boards 14 inside fixed culture dish rotate the position department of observing notch 15 through observing notch 15, the experimenter can observe the culture dish below of No. two transparent glass boards 14 inside fixes through observing notch 15, the convenience of experimenter observation No. two transparent glass boards 14 culture dish below has been guaranteed.
In addition, the device is bonded with a plurality of fixing blocks 17 on two sides of the upper surface of the control center 1, one side of the outer surface of each fixing block 17 is connected with a limiting block 18 in a clamping mode, the clamping connection part is fixed by bolts, the limiting block 18 can limit the culture chamber 3, the stability of the culture chamber 3 placed on the upper surface of the control center 1 is guaranteed, the position of the limiting block 18 can be adjusted in a loosening mode, the culture chamber 3 with different sizes can be properly fixed, and the practicability of the device is further improved.
This device cultivates 3 inside one sides of room and has seted up the notch, is convenient for through the notch to the device of cultivateing 3 inside cell culture of room, and the department passes through hinge rotation connection sealing door 2 in notch position simultaneously, and sealing door 2 is connected with the notch block to be convenient for cultivate 3 notch departments of room closed and open, sealing door 2 has the rubber pad with 3 notch block parcels of cultivateing the room simultaneously, further guarantees sealing door 2 and cultivates the leakproofness at 3 notch block edges of room.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. Hypoxic cell culture device, comprising a control centre (1) and a culture chamber (3), characterized in that: control center (1) surface both sides all are through No. two fixed block (8) of screw fixedly connected with, it is provided with bracing piece (6) to cultivate room (3) top, it has a plurality of fixed block (4) all to bond to cultivate room (3) upper surface both sides, No. two inside one side of fixed block (8) closes soon and is connected with adjusting bolt (9), adjusting bolt (9) surface one side end is rotated through bearing (16) and is connected with No. two bracing pieces (7), it has a transparent glass board (10) to bond to cultivate room (3) inside one side, and has seted up a plurality of recess on one side of a transparent glass board (10) inside, a transparent glass board (10) inside bonds and has a plurality of spacing spring assembly (11), a transparent glass board (10) top is provided with No. two transparent glass boards (14).
2. A hypoxic cell culture apparatus, according to claim 1, wherein: cultivate room (3) inside top and rotate and be connected with control lever (13), and control lever (13) surface one side end bonds and have No. three fixed blocks (12), No. three fixed block (12) surface both sides all bond and have No. two transparent glass board (14), observation notch (15) have been seted up to inside one side of a transparent glass board (10).
3. A hypoxic cell culture apparatus, according to claim 1, wherein: four fixing blocks (17) are bonded on two sides of the upper surface of the control center (1), and a limiting block (18) is connected to one side of the outer surface of each four fixing block (17) in a clamping mode.
4. A hypoxic cell culture apparatus, according to claim 1, wherein: one side in the culture chamber (3) is rotatably connected with a sealing door (2) through a plurality of hinges.
5. A hypoxic cell culture apparatus, according to claim 1, wherein: a handle rod (5) is welded on one side of the outer surface of the first fixing block (4).
6. A hypoxic cell culture apparatus, according to claim 1, wherein: the tail end of one side of the outer surface of the second support rod (7) is clamped and connected inside the first support rod (6).
7. A hypoxic cell culture apparatus, according to claim 1, wherein: and the tail ends of one sides of the outer surfaces of the adjusting bolt (9) and the control rod (13) are provided with rotating handles.
8. A hypoxic cell culture apparatus, according to claim 1, wherein: the inner part of the culture chamber (3) is connected with a nitrogen cylinder (19) and a carbon dioxide cylinder (20) through hoses.
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