CN209368302U - Multifunctional automatic independent/combined co-culture device - Google Patents
Multifunctional automatic independent/combined co-culture device Download PDFInfo
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- CN209368302U CN209368302U CN201822231837.3U CN201822231837U CN209368302U CN 209368302 U CN209368302 U CN 209368302U CN 201822231837 U CN201822231837 U CN 201822231837U CN 209368302 U CN209368302 U CN 209368302U
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- 239000007788 liquid Substances 0.000 claims abstract description 46
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 29
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 23
- 239000001963 growth medium Substances 0.000 claims abstract description 22
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- 238000010438 heat treatment Methods 0.000 claims description 44
- 239000000523 sample Substances 0.000 claims description 33
- 238000009413 insulation Methods 0.000 claims description 16
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical group [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 9
- 229910052782 aluminium Inorganic materials 0.000 claims description 9
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- 241000790917 Dioxys <bee> Species 0.000 claims 1
- GCNLQHANGFOQKY-UHFFFAOYSA-N [C+4].[O-2].[O-2].[Ti+4] Chemical compound [C+4].[O-2].[O-2].[Ti+4] GCNLQHANGFOQKY-UHFFFAOYSA-N 0.000 claims 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a multi-functional automatic independent/combined co-culture device, include: more than two culture units, culture dish combination and carbon dioxide inlet pipes which are connected through connecting pipelines. Using the co-culture device of the present application, different tissues or cells are placed in the respective culture unit internal dishes, the flow exchange channel is connected and appropriate passing filter membranes are selected as required for the experiment. Setting the culture temperature and CO required by different culture units2Concentration and timing sample collection time, etc.After the experiment is finished, the culture dish in the unit can be replaced, and the culture dish can also be taken out to carry out extraction operation of RNA, DNA and protein samples and the like. Each culture unit can independently and automatically collect samples at fixed time and replace culture media through the liquid flow control area, the defects that manual sample collection is complicated, time points are difficult to control and the like are overcome, the reliability of sample collection sample quality can be ensured to the maximum degree, and the detection accuracy of related indexes is improved.
Description
Technical field
The invention belongs to technical field of cell culture, in particular to a kind of multifunction automatic independence/joint co-cultures dress
It sets.
Background technique
In recent years, with stem-cell research, the development of regenerative medicine, it is desirable that largely modulate cell.Cell is in incubation
Carbon dioxide, waste is discharged in ingredient required for middle absorption oxygen, nutrient etc. are developed.Thus, due in long time cultivation cell
Culture medium can deteriorate, it is therefore desirable to regularly replace culture medium, the degradation of the necessary running audit culture medium of operating personnel.But
It is that operating personnel needs to be removed or placed into sample relative to incubator, at this point, to thin to check the degradation of culture medium
The impact iso-stress that born of the same parents generate when being applied to the environmental changes such as temperature, carrying, it is possible to which the development of cell is had an impact.
To traditional single layer face cell culture is used in current external toxicity and pharmacological experiment, which can not
Internal microenvironment locating for true simulation cell, may cause certain deviation when Study of Exogenous substance influences body.
In order to preferably establish the cultivating system similar to vivo environment, the late 1980s, researcher is single in original two dimension
Cell Co culturing Techenique has been developed on the basis of confluent monolayer cells culture.
Such as Chinese patent CN201410508108.1 discloses a kind of cell co-culture device, it includes pedestal, culture
The mobile cell culture chamber of base agitated assemblies, the interior culture dish for filling culture medium and multiple accommodating cells, multiple mobile cell trainings
Feeding room is movably mounted in culture dish, be provided on mobile cell culture chamber its inner cell cannot by and acellular can
By penetrating film, mobile cell culture chamber is separated by by penetrating film with culture dish, and culture medium agitated assemblies are mounted on the base,
And the rotary body in culture medium agitated assemblies protrudes into culture dish to stir the culture in culture dish when the rotating body is rotated
Base is transmitted to the cell secreta in mobile cell culture room on the target cell in corresponding mobile cell culture room.
But existing cell co-culture device with the following drawback that: (1) difference cultivation temperature needed for different tissues or cell
And CO2When concentration, existing co-culture device be cannot achieve；(2) when it is cumbersome manually to receive sample complexity, and being extremely difficult to collect between sample
Between accuracy and uniformity；(3) during tissue or Cell Co culturing Techenique are studied at present, many kinds of substance coexists in cultivating system, nothing
Method carries out independent studies to specific small-molecule substance effects certain in co-culture system；(4) existing co-culture device will be a variety of thin
The effects of born of the same parents are put in same cultivating system, cannot achieve " one-to-one " " single pair is more " and " multipair list " is studied；(5) existing total training
Feeding device is mainly by the way that Various Tissues or cell to be inoculated in same culture vessel simultaneously, or by penetrating film by two kinds of differences
Cell is nested together, and not only to change liquid process cumbersome for cell for these methods, and subsequent experimental is inconvenient (as single in separated and extracting
Cell RNA, DNA or albumen etc.).
Summary of the invention
The purpose of the present invention is in view of the drawbacks of the prior art, provide a kind of multifunction automatic independence/joint co-cultivation
Device.
To achieve the goals above, the invention adopts the following technical scheme: a kind of multifunction automatic independence/joint is trained altogether
Support device comprising: more than two independent culture unit, culture dishes combination and carbon dioxide connected by connecting pipe into
Feed channel；Wherein the independent culture unit from top to bottom successively includes temperature-controlled area, liquid stream control zone and low-temperature storage area；
The surrounding of the temperature-controlled area and the lower half portion inside connecting pipe are provided with heating and thermal insulation set, and culture dish combination is placed
In heating and thermal insulation set, the culture dish combination includes culture dish with cover identical with culture number of unit and connect two trainings
It supports the controllable liquid stream between ware and exchanges pipe, the controllable liquid stream exchange pipe exchanges component control by the controllable liquid stream being arranged in pipe
Liquid stream in tubulation flow to direction and can by substance, the bottom of the culture dish is provided with direct heater；It is described
Culture medium storage tanks and recycling can are placed in low-temperature storage area, the culture medium storage tanks and recycling can are respectively arranged with culture
Base tube road and recovery pipe, the pipeline pass through low-temperature storage area, reach at the top of culture dish, protrude into training across the lid of culture dish
It supports in ware, micropump is provided on pipeline, the micropump is placed in liquid stream control zone；
One end of the carbon dioxide pipe passes through culture dish lid and is connected to each independent culture dish, and the other end passes through two
Carbonoxide general pipeline connection carbon dioxide bottle.
Further, the section of controllable liquid stream exchange pipe is circle, and bottom is connected with the bottom of culture dish, the circle in section
The heart is concordant at the top of culture dish or is higher than at the top of culture dish.
Further, controllable liquid stream exchange component includes circular valve plate and the central axis that valve plate center is arranged in and rotation
Axis, the circular valve plate are averagely divided into two parts or three parts, and each section is respectively arranged with the filter of different selection passabilities
Film；The central axis tail portion is provided with worm gear mechanism, connects rotation axis；The rotation axis pass through controllable liquid stream exchange pipe and
Connecting pipe stretches out outside, and rotation axis rotation drives central axis to rotate circular valve plate by worm gear mechanism.
Further, controllable liquid stream exchange component further includes timing control device, and the timing control device includes timing
Button, timing controller and micromotor；The timing button is used for basis for control time, the timing controller to be arranged
Set time time opening and closing micromotor, the tail portion of the shaft of micromotor are provided with fixing sleeve, and rotation axis is inserted
Enter fixed interplanting, the rotation of micromotor drives rotation axis rotation.
Further, heating and thermal insulation set includes internal heat-conducting layer, middle part dielectric layer and external insulation heating layer, described thermally conductive
Layer is made of metal material, and priming petock in dielectric layer, the Heat preservation layer includes insulating layer, heating layer and biography from outside to inside
Thermosphere, the insulating layer are made of thermal insulation material, and heating layer is heating wire, and the heating wire connects muff temperature controller, are passed
Thermosphere is made of metal material.
Further, direct heater is aluminium heating plate, and the temperature controller of the aluminum heater connects temperature-sensitivprobe probe, the temperature
In quick probe insertion culture dish, temperature feedback is carried out.
Further, the quantity for cultivating unit is 2~4.
Further, temperature is controlled in low-temperature storage area at -20~4 DEG C.
Further, be provided with air intake control valve on carbon dioxide pipe, control different culture dish carbon dioxide into
Tolerance.
Further, it is provided with liquid crystal display outside independent culture unit, the liquid crystal display connects heating and thermal insulation
Set, direct heater, micropump and carbon dioxide pipe air intake control valve, for set heating and thermal insulation set and directly heat
Heating temperature, culture dish liquid feeding and the air inflow for receiving sample time, carbon dioxide of device, and the above-mentioned actual value of real-time display.
Using the co-culture device of the application, different tissues or cell are put into respectively culture unit interior dish, connection
Good liquid stream exchanges pipeline and according to the suitable permeability filter membrane of selection needed for experiment.Training is respectively placed inside three culture units
Required different culture medium and receipts sample pipe etc. are supported, system sealing is kept.Needed for the setting of different culture units can be set respectively
Cultivation temperature, CO2Sample time etc. is received in concentration and timing.Can be taken off during co-cultivation receive sample complete sample tube carry out storage sample and
Measurement etc., and can be according to needed for experiment, selecting different permeability filter membranes to carry out co-culture experiments researchs etc..It, can after the completion of experiment
Culture dish in unit is replaced, also can use trip RNA, DNA and protein sample extraction operation etc..Each culture unit can lead to
Cross liquid stream control zone and carry out independent self-timing and receive the operation such as sample and replacement culture medium, be conducive to improve it is artificial receive sample triviality and
Time point is difficult to the disadvantages of control.And unit bottom is equipped with low-temperature storage area (- 20~4 DEG C), can utmostly ensure to receive sample
The reliability of sample quality improves the detection accuracy of index of correlation.
Had using the co-culture device system of the application below the utility model has the advantages that (1) Various Tissues or cell are independently trained
Support temperature and CO2Concentration setting, can be applied to cultivate repercussion study between the different various kinds of cell of required condition；(2) pass through
Setting time utmostly ensures to receive all quality reliabilities, mentions in the automatic receipts sample of different time sections timing and cryo-conservation
High index of correlation detection accuracy, and can realize with sample after a collection of cell different time sections and different disposal collect automatically and after
Continuous detection；(3) automatic to replace liquid stream valve filter membrane by setting processing and incubation time, realize liquid flow direction and permeability
Control realizes different time sections, is different by " one-to-one ", " single pair is more " and " multipair list " Effect study of medium etc.；(4) lead to
Replacement permselectivity filter membrane is crossed, certain specific small molecule study on mechanism etc. in co-culture system is realized, can also pass through
Specific molecular absorption etc., reversely studies effect of the specific small molecule in co-culture system；(5) training inside the co-culture device
Supporting ware and pipeline independently can take out and replace, and subsequent experimental is facilitated to operate (such as separating and extract unicellular DNA or albumen)；
(6) co-culture device can realize Various Tissues or the cell-cell interaction research of different culture medium, and it is thin to carry out single
Born of the same parents' timing automatic replaceable liquid operation, it is convenient and efficient and do not influence other co-culture the normal cultivation conditions of tissue/cell；
Detailed description of the invention
Fig. 1 is the top view figure of embodiment multifunction automatic independence/joint co-culture device.
Fig. 2 is the structural schematic diagram of embodiment culture unit (wherein the heating and thermal insulation set of temperature-controlled area is cross-sectional view).
Fig. 3 is the stereoscopic schematic diagram of embodiment culture unit.
Fig. 4 is the partial schematic diagram of embodiment culture dish combination.
Fig. 5 is the schematic diagram at embodiment valve plate center.
Fig. 6 is the structural schematic diagram of the controllable liquid stream exchange component of embodiment.
Specific embodiment
In order to make those skilled in the art more fully understand application scheme, below in conjunction in the embodiment of the present application
Attached drawing, the technical scheme in the embodiment of the application is clearly and completely described, it is clear that described embodiment is only
The embodiment of the application a part, instead of all the embodiments.Based on the embodiment in the application, ordinary skill people
Member's every other embodiment obtained without making creative work, all should belong to the model of the application protection
It encloses.
It should be noted that term " includes " and " tool in the description and claims of this application and above-mentioned attached drawing
Have " and their any deformation, it is intended that cover it is non-exclusive include, for example, containing a series of steps or units
Process, method, system, product or equipment those of are not necessarily limited to be clearly listed step or unit, but may include without clear
Other step or units listing to Chu or intrinsic for these process, methods, product or equipment.
Multifunction automatic independence/joint co-culture device as shown in Figure 1 includes three as shown in Figure 1 and passes through connection
Culture unit 2, culture dish combination 3 and the carbon dioxide pipe 4 that pipeline 1 connects.
Wherein each culture unit from top to bottom successively includes temperature-controlled area 5,6 and of liquid stream control zone as shown in Figures 2 and 3
Low-temperature storage area 7.The surrounding of temperature-controlled area and the lower half portion inside connecting pipe are provided with heating and thermal insulation set, culture dish
Combination is placed in heating and thermal insulation set, and connecting pipe section is circle, can be opened between upper and lower pipeline, lower half portion pipeline and
Temperature-controlled area is connected, and can open top half pipeline and take out culture dish combination.
Heating and thermal insulation set includes internal heat-conducting layer 8, middle part dielectric layer 9 and external insulation heating layer.Heat-conducting layer uses metal
Material is made, priming petock in dielectric layer.Heat preservation layer includes insulating layer 10, heating layer 11 and heat transfer layer 12 from outside to inside.It protects
Warm layer is made of thermal insulation material, and heating layer is heating wire, and heating wire connects muff temperature controller, and heat transfer layer uses metal material
It is made.
Culture dish combination as shown in Figure 4 can draining including three culture dishes 13 with cover and for connect two culture dishes
The lower half portion of stream exchange pipe 14, culture dish and controllable liquid stream exchange pipe is placed in heating and thermal insulation set.Low-temperature storage is put in area
Culture medium storage tanks 15 and recycling can 16 are equipped with, culture medium storage tanks and recycling can are respectively arranged with culture medium pipeline 17 and recycling
Pipeline 18, pipeline pass through low-temperature storage area, reach at the top of culture dish, and the lid 19 across culture dish protrudes into culture dish, pipeline
On be provided with micropump (20a and 20b), micropump is peristaltic pump, and peristaltic pump is placed in liquid stream control zone.
The section of controllable liquid stream exchange pipe is circle, and bottom is connected with the bottom of culture dish, the center of circle in section and culture
It is concordant at the top of ware.Controllably the setting of liquid stream exchange component 21 is in controllable liquid stream exchange pipe, including circular valve plate 22 and setting are in valve
The central axis 23 and rotation axis 24 at plate center, circular valve plate are averagely divided into two parts, and each section is respectively arranged with different choosings
The filter membrane (25a and 25b) of passability is selected, as shown in Figure 5.Filter membrane can be unidirectional permeability filter membrane, bidirectional permeability filter membrane, no
Permeable filter membrane, infiltration-diffused filter membrane, Selective adsorption filter membrane or Preferential adsorption-pore flow model filter membrane are wherein
Two kinds.Central axis tail portion is provided with worm gear mechanism 26, connects rotation axis.Rotation axis passes through controllable liquid stream exchange pipe and connects
Adapter tube road stretches out outside, and rotation axis rotation drives central axis to rotate circular valve plate by worm gear mechanism.
Controllable liquid stream exchange component further includes timing control device 27, and timing control device includes that timing is pressed as shown in Figure 6
Button 28, timing controller 29 and micromotor 30.Timing button, timing controller and micromotor pass through circuit connection.
Timing button is used for for control time, timing controller to be arranged according to set time time opening and closing
Micromotor, the tail portion of the shaft of micromotor are provided with fixing sleeve 31, the fixed interplanting of rotation axis insertion, the rotation of micromotor
Drive rotation axis rotation.
Rotation rotation axis rotation circular valve plate can be can be manually rotated, timing control device, timing controlled rotation can also be passed through
Turn circular valve plate.
The bottom of culture dish is provided with aluminium heating plate 32, and the temperature controller of aluminium heating plate connects temperature-sensitivprobe probe 33, temperature-sensitivprobe probe
It is inserted into culture dish from culture dish lid, carries out temperature feedback.
One end of carbon dioxide pipe is connected to a culture dish, and the other end bout of three air inlet pipe passes through carbon dioxide
The connection carbon dioxide bottle of general pipeline 34.It is provided with air intake control valve 35 on carbon dioxide pipe, controls the air inflow of carbon dioxide.
It is provided with liquid crystal display 36 outside each independent culture unit, liquid crystal display connects heating and thermal insulation set, respectively
Aluminium heating plate, culture medium pipeline and micropump, carbon dioxide pipe in recovery pipe air intake control valve, for setting
The heating temperature of heating and thermal insulation set and aluminium heating plate, culture dish liquid feeding and the air inflow for receiving sample time, carbon dioxide, and in real time
Show above-mentioned actual value.
Entire co-culture device connects power supply by electric wire.
This multifunction automatic co-cultures the training of the tissue for being suitble to various different fostering requirements, primary cell and cell line
Support, can be widely applied to research various kinds of cell and cell-cell interaction relationship, be also suitable for research directly/mediate contact and non-
The co-culture systems such as contact.
This co-culture system is mainly by three independent cultures unit (A, B, C), controllability liquid stream exchange pipeline and independent CO2
Air inlet pipe is formed.Each culture unit has independent temperature control zone, liquid stream control zone and low-temperature storage area etc..
By taking unit A as an example, top is culture dish (can be taken off and replace), is temperature-controlled area close to one layer of culture dish, can
To be independently arranged specific temperature required for tissue or cell culture by aluminium heating plate, middle layer is liquid stream control zone, including
Timing recycles supernatant in culture dish and is automatically replenished the functions such as new culture medium；The bottom is low-temperature storage area, is mainly used for training
The temperature of base storage is supported at 4 DEG C, the sample of recycling is stored in -20 DEG C.Entire co-culture system outer layer cardinal temperature is maintained by water
Jacketing heat component is completed, and three different culture units have respective independent temperature heating systems, meet different tissues and
Needed for cell culture condition.
Connected between this co-culture system different units mainly by controllability liquid stream exchange pipeline completed, wherein pipeline compared with
Short, for length in 6~8cm, controllability liquid stream, which exchanges, has circular valve plate in pipeline, and there are two types of different selections to pass through for circular valve plate tool
The filter membrane of property, can be by operation center's axis, and the different filter membrane of control selections is interposed between different culture dishes, and also may be selected
Different molecular sieves studies interaction of the variety classes small molecule in variety classes cell culture.
This system difference culture unit has respective independent CO2Admission line, can by duct control system, to different tissues and
Different CO needed for the culture such as cell2Concentration is regulated and controled, and a variety of co-cultivation requirements are met.
Using the co-culture device of the application, different tissues or cell are put into respectively culture unit interior dish, connection
Good liquid stream exchanges pipeline and according to the suitable permeability filter membrane of selection needed for experiment.Training is respectively placed inside three culture units
Required different culture medium and receipts sample pipe etc. are supported, system sealing is kept.To the different respective required culture temperature of culture unit setting
Degree, CO2Sample time etc. is received in concentration and timing.It can be taken off receiving sample tube progress storage sample and measurement etc. that sample is completed during co-cultivation,
And it can be according to needed for experiment, selecting different permeability filter membranes to carry out co-culture experiments researchs etc..It, can be in unit after the completion of experiment
Culture dish is replaced, and also can be taken off carrying out RNA, DNA and protein sample extraction operation etc..Each culture unit can pass through liquid stream
Control zone carries out independent self-timing and receives the operation such as sample and replacement culture medium, is conducive to improve and manually receives sample triviality and time point
The disadvantages of being difficult to control.And unit bottom is equipped with low-temperature storage area (- 20~4 DEG C), utmostly can ensure to receive all qualities
The reliability of amount improves the detection accuracy of index of correlation.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any to repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of multifunction automatic independence/joint co-culture device, characterized by comprising: two or more passes through connecting pipe
Independent culture unit, culture dish combination and the carbon dioxide pipeline of connection；
Wherein the independent culture unit from top to bottom successively includes temperature-controlled area, liquid stream control zone and low-temperature storage area；Institute
It states the lower half portion inside the surrounding and connecting pipe of temperature-controlled area and is provided with heating and thermal insulation set, culture dish combination is placed on
In heating and thermal insulation set, the culture dish combination includes culture dish with cover identical with culture number of unit and connect two cultures
Controllable liquid stream between ware exchanges pipe, and the controllable liquid stream exchange pipe exchanges component control by the controllable liquid stream being arranged in pipe
Liquid stream in pipe flow to direction and can by substance, the bottom of the culture dish is provided with direct heater；It is described low
Culture medium storage tanks and recycling can are placed in warm storage area, the culture medium storage tanks and recycling can are respectively arranged with culture medium
Pipeline and recovery pipe, the pipeline pass through low-temperature storage area, reach at the top of culture dish, protrude into culture across the lid of culture dish
In ware, micropump is provided on pipeline, the micropump is placed in liquid stream control zone；
One end of the carbon dioxide pipe passes through culture dish lid and is connected to each independent culture dish, and the other end passes through titanium dioxide
Carbon general pipeline connection carbon dioxide bottle.
2. multifunction automatic independence/joint co-culture device according to claim 1, it is characterised in that: described controllable
The section that liquid stream exchanges pipe is circle, and bottom is connected with the bottom of culture dish, the center of circle in section it is concordant with culture dish top or
Higher than at the top of culture dish.
3. multifunction automatic independence/joint co-culture device according to claim 1, it is characterised in that: described controllable
It includes circular valve plate and the central axis and rotation axis that valve plate center is arranged in that liquid stream, which exchanges component, and the circular valve plate is averagely divided
At two parts or three parts, each section is respectively arranged with the filter membrane of different selection passabilities；The central axis tail portion is provided with
Worm gear mechanism connects rotation axis；The rotation axis passes through controllable liquid stream exchange pipe and connecting pipe stretches out external, rotation axis
Rotation drives central axis to rotate circular valve plate by worm gear mechanism.
4. multifunction automatic independence/joint co-culture device according to claim 3, it is characterised in that: described controllable
It further includes timing control device that liquid stream, which exchanges component, and the timing control device includes timing button, timing controller and miniature
Motor；The timing button is used for for control time, the timing controller to be arranged according to set time time opening
With closing micromotor, the tail portion of the shaft of micromotor is provided with fixing sleeve, and rotation axis insertion is fixed to interplant, micromotor
Rotation drives rotation axis rotation.
5. multifunction automatic independence/joint co-culture device according to claim 1, it is characterised in that: the heating
Muff includes internal heat-conducting layer, middle part dielectric layer and external insulation heating layer, and the heat-conducting layer is made of metal material, and is situated between
Priming petock in matter layer, the Heat preservation layer include insulating layer, heating layer and heat transfer layer from outside to inside, and the insulating layer is using guarantor
Adiabator is made, and heating layer is heating wire, and the heating wire connects muff temperature controller, and heat transfer layer is made of metal material.
6. multifunction automatic independence/joint co-culture device according to claim 1, it is characterised in that: described direct
Heater is aluminium heating plate, and the temperature controller of the aluminium heating plate connects temperature-sensitivprobe probe, and the temperature-sensitivprobe probe is inserted into culture dish, into
Trip temperature feedback.
7. multifunction automatic independence/joint co-culture device according to claim 1, it is characterised in that: the culture
The quantity of unit is 2~4.
8. multifunction automatic independence/joint co-culture device according to claim 1, it is characterised in that: the low temperature
Temperature is controlled in storage area at -20~4 DEG C.
9. multifunction automatic independence/joint co-culture device according to claim 1, it is characterised in that: the dioxy
Change and be provided with air intake control valve in carbon air inlet pipe, controls the air inflow of different culture dish carbon dioxide.
10. multifunction automatic independence/joint co-culture device according to claim 9, it is characterised in that: the independence
Be provided with liquid crystal display outside culture unit, the liquid crystal display connection heating and thermal insulation set, direct heater, micropump and
The air intake control valve of carbon dioxide pipe adds for setting heating and thermal insulation set and the heating temperature of direct heater, culture dish
Liquid and the air inflow for receiving sample time, carbon dioxide, and the above-mentioned actual value of real-time display.
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| CN201822231837.3U CN209368302U (en) | 2018-12-28 | 2018-12-28 | Multifunctional automatic independent/combined co-culture device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401971A (en) * | 2018-12-28 | 2019-03-01 | 江苏省人民医院（南京医科大学第附属医院） | Multifunctional automatic independent/combined co-culture device |
| WO2024041442A1 (en) * | 2022-08-22 | 2024-02-29 | 国科温州研究院(温州生物材料与工程研究所) | Cell culture device, system, and method |
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2018
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401971A (en) * | 2018-12-28 | 2019-03-01 | 江苏省人民医院（南京医科大学第附属医院） | Multifunctional automatic independent/combined co-culture device |
| CN109401971B (en) * | 2018-12-28 | 2024-01-30 | 江苏省人民医院（南京医科大学第一附属医院） | Multifunctional automatic independent/combined co-culture device |
| WO2024041442A1 (en) * | 2022-08-22 | 2024-02-29 | 国科温州研究院(温州生物材料与工程研究所) | Cell culture device, system, and method |
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