CN102199537B - Membrane bioreactor used in microgravity environment and simulated microgravity environment - Google Patents

Membrane bioreactor used in microgravity environment and simulated microgravity environment Download PDF

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Publication number
CN102199537B
CN102199537B CN 201110091329 CN201110091329A CN102199537B CN 102199537 B CN102199537 B CN 102199537B CN 201110091329 CN201110091329 CN 201110091329 CN 201110091329 A CN201110091329 A CN 201110091329A CN 102199537 B CN102199537 B CN 102199537B
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microgravity
bio
main body
parameter measurement
reactor main
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CN102199537A (en
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李春
王超
李坤鹏
滕杰
蒋远大
邓玉林
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Beijing Institute of Technology BIT
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

Abstract

The invention relates to a membrane bioreactor device for the efficient culture of suspended biological cells and in particular relates to a membrane bioreactor used in the microgravity environment and the simulated microgravity environment. The membrane bioreactor is mainly used in the microgravity environment and used as a ground simulated microgravity experimental device to perform the long-term culture of suspended biological cells and the biological effect research and the efficient preparation method research of recombinant protein. The membrane bioreactor comprises a bioreactor main body, an air supply system, a liquid filling system, a stirring system, a parameter measurement and control system, an exhaust system and a sampling system, wherein the parameter measurement and control system comprises a pH value parameter measurement and control system, a temperature parameter measurement and control system, a dissolved oxygen parameter measurement and control system and a pressure parameter measurement and control system. The membrane bioreactor device can be utilized to realize the long-term and efficient culture of suspended biological cells in the microgravity environment and the ground simulated microgravity environment, the multi-parameter real-time detection and the automatic control.

Description

Membrane bioreactor under a kind of microgravity and stimulated microgravity
Technical field
The present invention relates to the film bioreactor device that a kind of suspended biological cell high-efficient is cultivated, be particularly related to a kind ofly for the membrane bioreactor under microgravity and stimulated microgravity, be mainly used in microgravity environment and carry out the efficient preparation research of the long-time cultivation of suspended biological cell and biological effect research and recombinant protein as ground simulation microgravity experiment device.
Background technology
In the space microgravity environment, flow phenomenon is disappeared in gas and liquid, buoyancy disappears, and the component that different densities causes is separated and the sink-float phenomenon disappears, and the static pressure of fluid disappears etc.This makes gas-liquid-solid three-phase be difficult to separate, and has increased the supply of oxygen in the biomass cells culturing process, the discharge of waste gas and the uniform difficulty of mass transfer of nutrition.In order to carry out space microgravity environmental biomedicine effect and spatial cell biology, microbiological correlative study, must develop the biomass cells culture technique that is fit to the space microgravity environment, develop corresponding culture device.The astropowers such as the U.S., Russia, Europe, Japan carry out design and the space life science research of reactor for many years, make space biomass cells culture device and technology obtain significant progress.Some novel biomass cellss are cultivated unit hardware equipment and are come into operation, and can be used for from higher organism such as animal, plant to microorganism, cell, organize level, from the space biomedicine to different levels researchs such as Biotechnology In Spaces.Up to the present, rotary wall type has been developed in countries in the world, the continous pouring formula, the device that the microgravity environment biomass cells of the number of different types such as pulsed is cultivated carried out by the microgravity environment that is used for of just exploitation of NASA Johnson Space Center generation RWV (the Rotating Wall Vessel Bioreactor) reactor that biomass cells and tissue suspension are cultivated 90 years last century as the U.S..The U.S. that satisfies of NASA exploitation carries out the cell culture unit reactor (Cell CultureUnit Bioreactor, CCUB) that life science is studied on international space station.The single loop cell culture reactor (Single Loop for Cell Culture Bioreactor, SLCCB) of the development such as NASA's Ames Research Center etc.This serial reaction device system changes for exhaust mode and installs the series of measures such as defoaming device additional by outer circulation and solved preferably the deficiency that reactor assembly is cultivated biomass cells, can better adapt to the demand that the space microgravity environment carries out biomass cells culture studies and scientific experiment.
Under the space microgravity environment, biological scientific experiment has suitable challenge, these experiments need a large amount of time to go to prepare and hardware technology support reliably, therefore study microgravity environment and the impact of biomass cells is had very large restricted, comprise electric energy, weight, volume, loading restriction; Cosmonaut's restriction; Flying machine can limit; The commercial value restriction; Also need special experimental installation.Thereby need an energy to complete the equipment of simulated microgravity experiment on ground.Simulated microgravity is to utilize on the ground the simulated microgravity devices such as gyrator to make the successive randomly changing of the gravity direction that acts on object, it is " zero gravity " phenomenon that the vector of rotating a week (360 °) generation equals zero, the Cell and organism macromole is in a kind of simulation free falling body state, i.e. simulated microgravity state.Under stimulated microgravity, in fluid, shearing force and turbulent property reduce, and the settling ratio of organism changes.
Can simulate on the ground the device that produces microgravity has: the machine that certainly falls, aerostat, sounding rocket, the tower that falls, parabolic flight machine etc.But the shortcoming of these devices be can not be continuous the generation microgravity, for needing long-time continuous to produce the biological culture of microgravity effect and inapplicable.The simulated microgravity bio-reactor is that US National Aeronautics and Space Administration (NASA) develops for carrying out the spatial cell culture experiment the earliest, develops into gradually the culture apparatus of studying the biological effect under simulate microgravity such as animal and plant cells, microorganism under surface condition.Simultaneously, its type is also by the filling type cultured continuously of incipient simple batch cultivation to widespread use in front.Its function also develops into present multiparameter on-line monitoring regulation and control etc. by the initial rotating speed that can only simply regulate and control.Along with the fast development of space science technology, China had also developed the culture apparatus of cultivating animal and plant cells, tissue and various algae, microorganism etc. in the last few years.
But the different kind organism reactor of at present world's exploitation can not realize fully during under microgravity environment and ground simulation microgravity environment, biomass cells is cultivated gas-liquid mass transferring, the technical barrier such as separate, realize the high-efficient culture of biomass cells and monitoring and the regulation and control of correlation parameter.
Summary of the invention
The objective of the invention is to satisfy in order to solve biomass cells culture apparatus under microgravity environment that the suspended biological cell is long-time, the technical problem of high-efficient culture research, provide a kind of simple in structure, culture efficiency is high, can realize that the culture system multiparameter detects in real time and auto-control, long-time high-efficient culture, the multiparameter that can be used for carrying out under microgravity and ground simulation microgravity environment the suspended biological cell detects and the multifunctional membrane bio-reactor of auto-control in real time.
The objective of the invention is to be achieved through the following technical solutions.
Membrane bioreactor under a kind of microgravity of the present invention and stimulated microgravity, comprise bio-reactor main body, airing system, liquid filling system, stirring system, parameter measurement Controlling System, exhaust system and sampling system, wherein, parameter measurement Controlling System comprises pH value parameter Measurement and Control System, temperature parameter Measurement and Control System, dissolved oxygen parameter measurement Controlling System and pressure parameter Measurement and Control System.
The aspect ratio of bio-reactor main body is 1.5~2; The accumbency of bio-reactor main body is fixedly connected with on the bio-reactor base for supporting and with it; Bio-reactor main body top is provided with inlet mouth and first row gas port, and the left side wall body arranges fluid inlet, and the right side wall body is provided with leakage fluid dram and second row gas port from top to bottom, and outer side surface is provided with heating jacket; The air feed membrane module is coiled in the inwall side of bio-reactor main body by the support slot of bio-reactor main body inner wall; The cylinder spiral stirring rake is positioned on the central horizontal axis of bio-reactor main body with bio-reactor main body two side and is tightly connected; Central row air film device is positioned at cylinder spiral stirring rake and coaxial with it, and the cylinder spiral stirring rake rotates around central row air film device under power wheel drive.
Airing system is comprised of air feed bottle, reducing valve and gas meter; By controlling gas flow rate and gaseous tension, realize that in the oxygen supply membrane module, oxygen is to the still supply of nutrient solution, the oxygen supply membrane module is the poly tetrafluoroethylene pipe.
The liquid filling system is comprised of deformable Liquid storage bag, peristaltic pump and TT﹠C system; Wherein, the deformable Liquid storage bag comprises acid solution bag, alkali lye bag, nutrient solution bag and low temperature seed liquor bag, and peristaltic pump comprises acid solution conveying peristaltic pump, alkali lye conveying peristaltic pump, nutrient solution conveying peristaltic pump and seed liquor conveying peristaltic pump; Acid solution carries peristaltic pump to control the conveying of liquid in the acid solution bag, alkali lye carries peristaltic pump to control the conveying of liquid in the alkali lye bag, nutrient solution carries peristaltic pump to control the conveying of nutritive medium in the nutrient solution bag, and seed liquor carries peristaltic pump to control the conveying of liquid spawn in low temperature seed liquor bag; Working hour by controlling each peristaltic pump and flow are realized the accurate pulse filling of liquid; The temperature parameter Measurement and Control System is comprised of micro temperature sensor probe, heating jacket and TT﹠C system, and when in the bio-reactor main body, temperature was lower than preset temp, the heating jacket follow procedure was started working, and realizes the accurate control of temperature; Minisize pH sensor probe, micro temperature sensor probe, miniature dissolved oxygen sensor probe and the sealing of micro pressure sensor probe are arranged on the inwall of bio-reactor main body upper and lower.
Stirring system is comprised of magnetic force driving motor and cylinder spiral stirring rake, and wherein, the cylinder spiral stirring rake comprises cylinder spiral stirring rake base, magnetic sheet and cylinder spiral stirrer paddle, and drum surface is evenly distributed with sieve aperture.Utilize the rotation of magnetic force driving electric motor driving cylinder spiral stirring rake, can not cause on the one hand the fragmentation of cell walls for culturing micro-organisms, cell and tissue, realize the abundant mixing of gas-liquid-solid; The rotation of cylinder spiral stirrer paddle under the normal gravity of ground on the other hand is for the cultivation of biomass cells provides stimulated microgravity; The power system of stirring system can also be motor direct-drive.
Exhaust system is comprised of central row air film device, vacuum pump and gas sampling bag.
Sampling system is comprised of sampling peristaltic pump and sample collection pond.
Beneficial effect
Film bioreactor device of the present invention can realize that long-time high-efficient culture, the multiparameter of microgravity environment and ground simulation microgravity environment low suspension biomass cells detect and auto-control in real time.
Description of drawings
Fig. 1 is film bioreactor device embodiment schematic diagram of the present invention;
Fig. 2 is bio-reactor main body embodiment diagrammatic cross-section of the present invention;
Fig. 3 is cylinder spiral stirring rake embodiment diagrammatic cross-section of the present invention;
Wherein, 1-bio-reactor main body; 2-oxygen supply membrane module; 3-cylinder spiral stirring rake; 4-central row air film device; The 5-heating jacket; 6-bio-reactor base for supporting; The 7-air feed bottle; The 8-reducing valve; The 9-gas meter; 10-acid solution bag; 11-alkali lye bag; 12-nutrient solution bag; 13-low temperature seed liquor bag; The 14-acid solution is carried peristaltic pump; 15-alkali lye is carried peristaltic pump; The 16-nutrient solution is carried peristaltic pump; The 17-seed liquor is carried peristaltic pump; 18-minisize pH sensor probe; 19-micro temperature sensor probe; 20-magnetic force driving motor; The miniature dissolved oxygen sensor probe of 21-; 22-micro pressure sensor probe; The 23-peristaltic pump of taking a sample; 24-sample collection pond; The 25-vacuum pump; 26-gas sampling bag; The 101-fluid inlet; The 102-inlet mouth; 103-first row gas port; The 104-leakage fluid dram; 105-second row gas port; 301-cylinder spiral stirring rake base; The 302-magnetic sheet; 303-cylinder spiral stirrer paddle; The 304-sieve aperture.
Embodiment
Below in conjunction with drawings and Examples, the present invention is elaborated.
Embodiment
As shown in Figure 1, membrane bioreactor comprises bio-reactor main body, airing system, liquid filling system, stirring system, parameter measurement Controlling System, exhaust system and sampling system, wherein, parameter measurement Controlling System comprises pH value parameter Measurement and Control System, temperature parameter Measurement and Control System, dissolved oxygen parameter measurement Controlling System and pressure parameter Measurement and Control System.
Bio-reactor main body 1 adopts polycarbonate material to make, and aspect ratio is 1.5, and volume is 100ml.1 accumbency of bio-reactor main body is fixedly connected with on bio-reactor base for supporting 6 and with it; Bio-reactor main body 1 top is provided with inlet mouth 102 and first row gas port 103 as shown in Figure 2, and the left side wall body arranges fluid inlet 101, and the right side wall body is provided with leakage fluid dram 104 and second row gas port 105 from top to bottom, and outer side surface is provided with heating jacket 5; Air feed membrane module 2 is coiled in the inwall side of bio-reactor main body 1 by the support slot of bio-reactor main body inner wall; Cylinder spiral stirring rake 3 is positioned on the central horizontal axis of bio-reactor main body 1 with bio-reactor main body 1 two side and is tightly connected; Central row air film device 4 is positioned at cylinder spiral stirring rake 3 and coaxial with it, and cylinder spiral stirring rake 3 rotates around central row air film device 4 under the driving of magnetic force driving motor 20;
Airing system is comprised of air feed bottle 7, reducing valve 8 and gas meter 9, and the gas in air feed bottle 7 is air; By controlling gas flow rate 10-30ml/min and gaseous tension, realize the interior oxygen of oxygen supply membrane module 2 to the still supply of nutrient solution, oxygen supply membrane module 2 is the poly tetrafluoroethylene pipe.
The liquid filling system is comprised of deformable Liquid storage bag, peristaltic pump and TT﹠C system; Wherein, the deformable Liquid storage bag comprises acid solution bag 10, alkali lye bag 11, nutrient solution bag 12 and low temperature seed liquor bag 13, and peristaltic pump comprises acid solution conveying peristaltic pump 14, alkali lye conveying peristaltic pump 15, nutrient solution conveying peristaltic pump 16 and seed liquor conveying peristaltic pump 17; Acid solution carry peristaltic pump 14 control acid solution bag 10 interior liquid conveying, alkali lye carry peristaltic pump 15 control alkali lye bag 11 interior liquid conveying, nutrient solution carries peristaltic pump 16 to control the conveying of nutrient solution bag 12 interior nutritive mediums, and seed liquor carries peristaltic pump 17 to control the conveying of low temperature seed liquor bag 13 interior liquid spawns; Working hour by controlling each peristaltic pump and flow are realized the accurate pulse filling of liquid, and the power of peristaltic pump is 6W, and flow is 0.1-7.5ml/min; The temperature parameter Measurement and Control System is comprised of micro temperature sensor probe 19, heating jacket 5 and TT﹠C system, when the interior temperature of bio-reactor main body 1 during lower than preset temp, heating jacket 5 follow procedures are started working, and realize the accurate control of temperature, and heating jacket 5 power are 15W; Minisize pH sensor probe 18, micro temperature sensor probe 19, miniature dissolved oxygen sensor probe 21 and 22 sealings of micro pressure sensor probe are arranged on the inwall of bio-reactor main body 1 upper and lower and are an angle of 90 degrees with bio-reactor main body 1 bottom surface.
Stirring system is comprised of magnetic force driving motor 20 and cylinder spiral stirring rake 3, wherein, as shown in Figure 3, cylinder spiral stirring rake 3 comprises cylinder spiral stirring rake base 301, magnetic sheet 302 and cylinder spiral stirrer paddle 303, its spiral number of turns is 2 circles, and drum surface is evenly distributed with sieve aperture 304.Utilize magnetic force driving motor 20 to drive 3 rotations of cylinder spiral stirring rake, the power of magnetic force driving motor 20 is 12W, maximum stirring velocity is 60rpm, can not cause on the one hand the fragmentation of cell walls for culturing micro-organisms, cell and tissue, realizes the abundant mixing of gas-liquid-solid; The rotation of cylinder spiral stirrer paddle 3 under the normal gravity of ground on the other hand is for the cultivation of biomass cells provides stimulated microgravity.
Exhaust system is comprised of central row air film device 4, vacuum pump 25 and gas sampling bag 26.
Sampling system is comprised of sampling peristaltic pump 23 and sample collection pond 24.
Working process:
Need utilize alcohol immersion before carrying out the suspended biological cell cultures under microgravity and stimulated microgravity, uv irradiating, the modes such as sterilized water are carried out cold sterilization to reactor assembly and are processed and clean.Then carry out biomass cells and cultivate operation.at first, nutrient solution carries peristaltic pump 16 that the aseptic culture fluid in nutrient solution bag 12 is annotated in bio-reactor main body 1 with the speed of 7.5ml/min, carry 16 working hour of peristaltic pump 12min to make liquid be filled with bio-reactor main body 1 by controlling nutrient solution, the liquid that is filled with accounts for 90% of bio-reactor main body 1 volume, gas enters along in the oxygen supply membrane module 2 of bio-reactor main body 1 inwall coiling through air feed bottle 7, reducing valve 8 and gas meter 9, by controlling gas flow rate 10-30ml/min and gaseous tension, realization improves oxygen utilization efficient to the still supply of bio-reactor main body 1 interior oxygen, during to the 1 culture system inoculation of bio-reactor main body, the low temperature seed liquor bag 13 of 4 ℃ carries peristaltic pump 17 to annotate to the interior pulsed of bio-reactor main body 1 under the control of supervisory system by seed liquor, carry the working hour 80s of peristaltic pump 17 by controlling seed liquor, realize that the seed liquor filling rate is 10ml, utilize power to drive the interior cylinder spiral stirring rake 3 of bio-reactor main body 1 around the speed rotation of central row air film device 4 with 30rpm for the magnetic force driving motor 20 of 12W, by rotatablely moving of cylinder spiral stirrer paddle 303 on cylinder spiral stirring rake 3, realize the even distribution (carry out biomass cells when cultivating the high-density culture that rotatablely moves as cell of cylinder spiral stirring rake 3 stimulated microgravity is provided) of bio-reactor main body 1 interior gas-liquid-solid three-phase under the normal gravity environment of ground, utilize the temperature parameter Measurement and Control System to control bio-reactor main body 1 inner suspension biomass cells and cultivate the stable of required microenvironment, when temperature sensor probe 19 detects the interior temperature of bio-reactor main body 1 and spends lower than preset temp 37, supervisory system is opened the switch of heating jacket 5 automatically, heating jacket 5 begins heating, when reaching preset temp, heating jacket 5 switch autoshutdowns are realized the accurate monitoring of temperature (± 0.5 degree), utilize the minisize pH sensor probe 18 that arranges on bio-reactor main body 1 upper and lower inwall, miniature dissolved oxygen sensor probe 21 and micro pressure sensor probe 22 are respectively to the pH value (2-12) of bio-reactor main body 1 inner suspension biomass cells fermentation culture system, oxygen dissolving value (20-150%) and force value (70-90KPa) carry out that real-time online detects and by predefined value, the working hour of carrying peristaltic pump 16 and alkali lye to carry peristaltic pump 17 by controlling acid solution, the accurate control of realization to culture system pH value, enter flow and the pressure of bio-reactor main body 1 by controlling oxygen, the accurate monitoring of realization to the culture system oxygen dissolving value.
When the suspended biological cell cultures was carried out sampling analysis, sampling place peristaltic pump 23 was started working and pump into cell cultures liquid until be full of collecting tank in sample collection pond 24.
In the middle and later periods of suspended biological cell cultures, the oxygen that the Growth of Cells metabolic exhaustion is a large amount of, producing a large amount of refuse oxygen dissolving values this moment also decreases, under the control of supervisory system, the vacuum pump 25 of exhaust system is started working, to the pipeline that connects central row air film device 4 take out negative pressure (≤0.08MPa), realize the rapidly and efficiently discharging of biomass cells metabolic waste carbon dioxide, reduce it to the restraining effect of Growth of Biologic Cell metabolism.
Above-described specific descriptions; purpose, technical scheme and beneficial effect to invention further describe; institute is understood that; the above is only specific embodiments of the invention; the protection domain that is not intended to limit the present invention; within the spirit and principles in the present invention all, any modification of making, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.

Claims (7)

1. membrane bioreactor under a microgravity and stimulated microgravity, it is characterized in that: comprise bio-reactor main body (1), airing system, liquid filling system, stirring system, parameter measurement Controlling System, exhaust system and sampling system, wherein, parameter measurement Controlling System comprises pH value parameter Measurement and Control System, temperature parameter Measurement and Control System, dissolved oxygen parameter measurement Controlling System and pressure parameter Measurement and Control System;
Bio-reactor main body (1) accumbency is gone up and is fixedly connected with it at bio-reactor base for supporting (6); Bio-reactor main body (1) top is provided with inlet mouth (102) and first row gas port (103); The left side wall body arranges fluid inlet (101), and the right side wall body is provided with leakage fluid dram (104) and second row gas port (105) from top to bottom, and outer side surface is provided with heating jacket (5); Oxygen supply membrane module (2) is coiled in the inwall side of bio-reactor main body (1) by the support slot of bio-reactor main body inner wall; Cylinder spiral stirring rake (3) is positioned on the central horizontal axis of bio-reactor main body (1) with bio-reactor main body (1) two side and is tightly connected; Central row air film device (4) is positioned at cylinder spiral stirring rake (3) and coaxial with it, and cylinder spiral stirring rake (3) rotates around central row air film device (4) under power wheel drive;
Airing system is comprised of air feed bottle (7), reducing valve (8) and gas meter (9);
The liquid filling system is comprised of deformable Liquid storage bag, peristaltic pump and TT﹠C system;
The temperature parameter Measurement and Control System is comprised of micro temperature sensor probe (19), heating jacket (5) and TT﹠C system, and minisize pH sensor probe (18), micro temperature sensor probe (19), miniature dissolved oxygen sensor probe (21) and micro pressure sensor probe (22) sealing are arranged on the inwall of bio-reactor main body (1) upper and lower;
Stirring system is comprised of power system and cylinder spiral stirring rake (3);
Exhaust system is comprised of central row air film device (4), vacuum pump (25) and gas sampling bag (26);
Sampling system is comprised of sampling peristaltic pump (23) and sample collection pond (24).
2. membrane bioreactor under a kind of microgravity according to claim 1 and stimulated microgravity, it is characterized in that: the aspect ratio of bio-reactor main body (1) is 1.5~2.
3. membrane bioreactor under a kind of microgravity according to claim 1 and stimulated microgravity, it is characterized in that: oxygen supply membrane module (2) is the poly tetrafluoroethylene pipe.
4. membrane bioreactor under a kind of microgravity according to claim 1 and stimulated microgravity, it is characterized in that: the deformable Liquid storage bag comprises acid solution bag (10), alkali lye bag (11), nutrient solution bag (12) and low temperature seed liquor bag (13), and peristaltic pump comprises acid solution conveying peristaltic pump (14), alkali lye conveying peristaltic pump (15), nutrient solution conveying peristaltic pump (16) and seed liquor conveying peristaltic pump (17).
5. membrane bioreactor under a kind of microgravity according to claim 1 and stimulated microgravity is characterized in that: minisize pH sensor probe (18), micro temperature sensor probe (19), miniature dissolved oxygen sensor probe (21) and micro pressure sensor probe (22) and bio-reactor main body (1) bottom surface are an angle of 90 degrees.
6. membrane bioreactor under a kind of microgravity according to claim 1 and stimulated microgravity, it is characterized in that: cylinder spiral stirring rake (3) comprises cylinder spiral stirring rake base (301), magnetic sheet (302) and cylinder spiral stirrer paddle (303), and drum surface is evenly distributed with sieve aperture (304).
7. membrane bioreactor under a kind of microgravity according to claim 1 and stimulated microgravity, it is characterized in that: the power system of stirring system is magnetic force driving or motor direct-drive.
CN 201110091329 2011-04-13 2011-04-13 Membrane bioreactor used in microgravity environment and simulated microgravity environment Expired - Fee Related CN102199537B (en)

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