CN112553073A - Composite culture tank for multi-cell culture - Google Patents
Composite culture tank for multi-cell culture Download PDFInfo
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- CN112553073A CN112553073A CN202011442800.0A CN202011442800A CN112553073A CN 112553073 A CN112553073 A CN 112553073A CN 202011442800 A CN202011442800 A CN 202011442800A CN 112553073 A CN112553073 A CN 112553073A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 17
- 239000002131 composite material Substances 0.000 title claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 16
- 238000005242 forging Methods 0.000 claims abstract description 4
- 239000001963 growth medium Substances 0.000 claims description 32
- 238000002347 injection Methods 0.000 claims description 25
- 239000007924 injection Substances 0.000 claims description 25
- 238000003860 storage Methods 0.000 claims description 24
- 238000010438 heat treatment Methods 0.000 claims description 19
- 239000007921 spray Substances 0.000 claims description 11
- 210000000078 claw Anatomy 0.000 claims description 7
- 238000005485 electric heating Methods 0.000 claims description 7
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- 230000000694 effects Effects 0.000 claims description 3
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/20—Heating; Cooling
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Abstract
The invention discloses a composite culture tank for multi-cell culture, which belongs to the field of cell culture devices and comprises a forging die, wherein a servo motor is fixedly connected on a bearing seat, a transparent cup is fixedly connected on the power output end of the servo motor, the top end of the transparent cup is clamped with a top cover, a pressure mechanism is fixedly connected on the top cover, a cover plate matched with the transparent cup is fixedly connected on the telescopic end of the pressure mechanism, two pairs of vertical guide rails are fixedly connected on the inner wall of the transparent cup, a middle plate is arranged in the transparent cup and is fixedly connected with the vertical guide rails, a culture seat is arranged on the lower side of the middle plate, two pairs of movable plates are fixedly connected on the culture seat, a plurality of culture mediums are clamped on the movable plates, an electric guide rail is fixedly connected on the outer wall of the bearing seat, the centralized culture of a large number of cells can be realized, and a healthy growth environment is provided for the cells which, and facilitates the display and centralized culture of a plurality of cell culture media.
Description
Technical Field
The invention relates to the field of cell culture devices, in particular to a composite culture tank for multi-cell culture.
Background
Pluripotent stem cells (embryonic stem cells and artificial pluripotent stem cells are considered as important cell sources for regenerative medicine due to their unlimited proliferation potential and multi-differentiation potential, and as regenerative medicine realized by stem cells, there are conceivable, for example, treatments for liver cirrhosis, blood diseases and myocardial infarction, construction of blood vessels, regeneration of bones and corneas, and securing of skin for transplantation.
In recent years, in the fields of pharmaceutical production, gene therapy, regenerative medicine, immunotherapy, and the like, there has been a demand for a cell culture apparatus that efficiently cultures a large amount of stem cells in an artificial environment.
Most of the existing cell culture methods store a culture medium in which cells are cultured in a proper environment for culturing, on one hand, the cells cannot be uniformly cultured with one pair of a plurality of kinds or a large amount of cells, and most of culture devices are closed in the culture process, so that the growth state of the cells is not easy to observe.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems in the prior art, the invention aims to provide a composite culture tank for multi-cell culture, which can realize centralized culture of a large number of cells, provide a healthy growth environment for the cells which grow in a centralized manner, and facilitate the display and centralized culture of a plurality of cell culture media.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A composite culture tank for multi-cell culture comprises a forging die, wherein a servo motor is fixedly connected to a bearing seat, a transparent cup is fixedly connected to the power output end of the servo motor, a top cover is clamped at the top end of the transparent cup, a pressure mechanism is fixedly connected to the top cover, a cover plate matched with the transparent cup is fixedly connected to the telescopic end of the pressure mechanism, two pairs of vertical guide rails are fixedly connected to the inner wall of the transparent cup, an intermediate plate is arranged in the transparent cup and fixedly connected with the vertical guide rails, a culture seat is arranged on the lower side of the intermediate plate, two pairs of movable plates are fixedly connected to the culture seat, a plurality of culture mediums are clamped on the movable plates, an electric guide rail is fixedly connected to the outer wall of the bearing seat, a clamping jaw is fixedly connected to the movable end of the electric guide rail and comprises a jaw frame, fixedly connected with two pairs of and vertical guide rail assorted cylinder electro-magnets on the claw frame, cylinder electro-magnet sliding connection is in vertical guide rail, the equal fixedly connected with in upper and lower both ends of cylinder electro-magnet and vertical guide rail assorted gyro wheel can realize concentrating the cultivation to a large amount of cells, for concentrating the cell of growing and provide healthy growing environment, and make things convenient for the show and the centralized culture of a plurality of cell culture medium.
Further, the intermediate plate includes the heat treatment ring, the joint has the mesh board on the inner wall of heat treatment ring, two pairs of through-holes have been dug on the mesh board, fixedly connected with heat accumulation board in the through-hole, the heat treatment ring is made by the heat conduction material, mesh board and heat accumulation board are insulation material, can realize keeping warm to the culture medium through the intermediate plate, can keep the constant temperature state for a long time after making the culture medium heating, and the cell is healthy to grow up in the easy culture medium.
Furthermore, the culture medium comprises a substrate, the middle part of the substrate is fixedly connected with a culture block, the distance between two adjacent culture blocks is 1-2cm, and the distance between the upper surface of the substrate and the upper surface of the culture block is 0.5-1cm, so that the two culture media have enough gap to ensure air flow.
Further, the upper end fixedly connected with two pairs of hollow columns of fly leaf, the top fixedly connected with and mesh board assorted check valve of hollow column, a plurality of evenly distributed's of fixedly connected with spacing ring on the hollow column, fixedly connected with spray tube on the spacing ring, the spray tube communicates with each other with hollow column, the fly leaf is made by the heat-insulating material, carries out the nutrient supply to the multilayer culture medium through hollow column water conservancy diversion nutritional agent.
Furthermore, the cover plate comprises an injection tray, an electric heating plate is fixedly connected to the injection tray, a plurality of injection nozzles matched with the one-way valves are fixedly connected to the bottom end of the injection tray, and the nutrient is supplied to the culture medium by stacking the injection nozzles on the cover plate and the one-way valves.
Further, the pressure mechanism includes the storage shell, sliding connection has the piston in the storage shell, the bottom fixedly connected with electric putter of storage shell, electric putter's flexible end and piston fixed connection, the top fixedly connected with and the storage shell matched with electric putter of piston, the bottom of storage shell communicates with each other with the injection shower nozzle and extrudes the culture solution in the storage shell and gets into in the apron.
Further, two pairs of fixed disks of servo motor's top fixedly connected with, the bottom fixedly connected with and the fixed disk assorted chuck of fly leaf, bear and install in the seat with servo motor assorted card post, the power take off end and the servo motor fixed connection of card post guarantee that the culture seat can fix firmly when descending to the bottom.
Furthermore, a diversion trench is drilled on the culture medium and extends to the bottom end of the cultivation block, so that the nutrient solution splashed out of the spray pipe flows to the bottom end of the cultivation block through the diversion trench
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) the scheme can realize concentrated cultivation of a large number of cells, provides a healthy growth environment for the cells which grow intensively, and is convenient for the display and the concentrated cultivation of a plurality of cell culture media.
(2) The intermediate plate includes the heat treatment ring, and the joint has the mesh board on the inner wall of heat treatment ring, and the last excavation of mesh board has two pairs of through-holes, fixedly connected with heat accumulation board in the through-hole, and the heat treatment ring is made by the heat conduction material, and mesh board and heat accumulation board are insulation material, can realize keeping warm to the culture medium through the intermediate plate, can keep the constant temperature state for a long time after making the culture medium heating, and the cell is healthy to grow up in the easy culture medium.
(3) The cover plate comprises an injection tray, an electric heating plate is fixedly connected to the injection tray, a plurality of injection nozzles matched with the one-way valves are fixedly connected to the bottom end of the injection tray, and the nutrient is supplied to the culture medium by stacking the injection nozzles on the cover plate and the one-way valves. The pressure mechanism comprises a storage shell, a piston is connected in the storage shell in a sliding mode, an electric push rod is fixedly connected to the bottom end of the storage shell, the telescopic end of the electric push rod is fixedly connected with the piston, the top end of the piston is fixedly connected with the electric push rod matched with the storage shell, the bottom end of the storage shell is communicated with an injection nozzle, a culture solution in the piston extrusion storage shell enters a cover plate, two pairs of hollow columns are fixedly connected to the upper end of a movable plate, the top end of each hollow column is fixedly connected with a one-way valve matched with a mesh plate, a plurality of uniformly distributed limiting rings are fixedly connected to each hollow column, spray pipes are fixedly connected to the limiting rings and communicated with the hollow columns, the movable plate is made of a heat-insulating material, and nutrient supply.
(4) Two pairs of fixed disks are fixedly connected to the top end of the servo motor, a chuck matched with the fixed disks is fixedly connected to the bottom end of the movable plate, a clamping column matched with the servo motor is installed in the bearing seat, and the power output end of the clamping column is fixedly connected with the servo motor, so that the culture seat can be fixed firmly when being descended to the bottom end.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is an exploded view of the present invention;
FIG. 3 is a cross-sectional view of the present invention;
FIG. 4 is a schematic view of the structure at A in FIG. 3;
FIG. 5 is a schematic view of the structure at B in FIG. 4;
FIG. 6 is an exploded view of the intermediate plate of the present invention;
fig. 7 is a cross-sectional view of a pressure mechanism of the present invention.
The reference numbers in the figures illustrate:
the culture device comprises a culture device 1, a servo motor 101, a transparent cup 2, a vertical guide rail 201, a top cover 3, a pressure mechanism 4, a storage shell 401, a piston 402, an electric push rod 403, a cover plate 5, a feeding disc 501, an electric heating plate 502, an injection nozzle 503, an intermediate plate 6, a heat treatment ring 601, a mesh plate 602, a heat storage plate 603, a culture seat 7, a movable plate 701, a hollow column 702, a check valve 703, a limiting ring 704, a spray pipe 705, a culture medium 8, an electric guide rail 9 and a clamping jaw 10.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Example 1:
referring to fig. 1-3, a composite culture tank for multi-cell culture comprises a forging die 1, a servo motor 101 is fixedly connected to a bearing seat 1, a transparent cup 2 is fixedly connected to a power output end of the servo motor 101, two pairs of fixed disks are fixedly connected to a top end of the servo motor 101, a chuck matched with the fixed disks is fixedly connected to a bottom end of a movable plate 701, a clamping column matched with the servo motor 101 is installed in the bearing seat 1, and a power output end of the clamping column is fixedly connected with the servo motor 101, so that the culture seat can be firmly fixed when being lowered to the bottom end.
Referring to fig. 1-4, a top cover 3 is fastened to the top of a transparent cup 2, a pressure mechanism 4 is fixedly connected to the top cover 3, a cover plate 5 matched with the transparent cup 2 is fixedly connected to the telescopic end of the pressure mechanism 4, two pairs of vertical guide rails 201 are fixedly connected to the inner wall of the transparent cup 2, an intermediate plate 6 is arranged in the transparent cup 2, the intermediate plate 6 is fixedly connected to the vertical guide rails 201, a culture base 7 is arranged on the lower side of the intermediate plate 6, two pairs of movable plates 701 are fixedly connected to the culture base 7, a plurality of culture mediums 8 are fastened to the movable plates 701, an electric guide rail 9 is fixedly connected to the outer wall of the carrying base 1, a clamping jaw 10 is fixedly connected to the movable end of the electric guide rail 9, the clamping jaw 10 comprises a claw frame, two pairs of cylindrical electromagnets matched with the vertical guide rails 201 are fixedly connected to the claw frame, the cylindrical electromagnets are slidably connected to the vertical guide rails 201, can realize the concentrated culture of a large amount of cells, provide healthy growth environment for the cells which grow in a concentrated way, and facilitate the display and the concentrated culture of a plurality of cell culture media.
Referring to fig. 4-7, the intermediate plate 6 includes a heat treatment ring 601, a mesh plate 602 is clamped on an inner wall of the heat treatment ring 601, two pairs of through holes are cut on the mesh plate 602, a heat storage plate 603 is fixedly connected in the through holes, the heat treatment ring 601 is made of a heat conductive material, and both the mesh plate 602 and the heat storage plate 603 are made of a heat insulating material, so that the intermediate plate 6 can insulate the culture medium 8, the culture medium 8 can be kept at a constant temperature for a long time after being heated, and cells in the culture medium 8 can grow healthily. Culture medium 8 includes base plate 801, the middle part fixedly connected with of base plate 801 cultivates piece 802, and the interval between two adjacent cultivates pieces 802 is 1-2cm, and the interval between the upper surface of base plate 801 and the upper surface of cultivateing piece 802 is 0.5-1cm, guarantees that two culture medium 8 have sufficient space and guarantees that the air flows, cuts a water conservancy diversion groove on culture medium 8, and the water conservancy diversion groove extends to the bottom of cultivateing piece 802, guarantees that the nutrient solution that splashes out from spray tube 705 flows to the bottom of cultivateing piece 802 through the water conservancy diversion groove.
Referring to fig. 2-5, two pairs of hollow columns 702 are fixedly connected to the upper end of the movable plate 701, a check valve 703 matched with the mesh plate 602 is fixedly connected to the top end of each hollow column 702, a plurality of spacing rings 704 are fixedly connected to the hollow columns 702 and uniformly distributed, a spray pipe 705 is fixedly connected to each spacing ring 704, the spray pipe 705 is communicated with the hollow columns 702, the movable plate 701 is made of a heat insulating material, and nutrients are guided by the hollow columns 702 to supply nutrients to the multi-layer culture medium 8.
Referring to fig. 3-6, the cover plate 5 includes a feeding tray 501, an electric heating plate 502 is fixedly connected to the feeding tray 501, a plurality of injection nozzles 503 matched with the one-way valves 703 are fixedly connected to the bottom end of the feeding tray 501, and the nutrient is supplied to the culture medium 8 by stacking the injection nozzles 503 on the cover plate 5 and the one-way valves 703.
Referring to fig. 5-7, the pressure mechanism 4 includes a storage shell 401, a piston 402 is slidably connected in the storage shell 401, an electric push rod 403 is fixedly connected to the bottom end of the storage shell 401, an extension end of the electric push rod 403 is fixedly connected to the piston 402, an electric push rod 403 matched with the storage shell 401 is fixedly connected to the top end of the piston 402, and the bottom end of the storage shell 401 is communicated with an injection nozzle 503, and the piston 402 extrudes the culture solution in the storage shell 401 into the cover plate 5.
When the device is used, collected cells are placed on a culture medium 8, the culture medium 8 is fixed on a culture seat 7, a heat treatment ring 601 is arranged on an intermediate plate 6, the upper end of the culture medium 8 is sealed and protected by a mesh plate 602, then an electric push rod in a pressure control mechanism 4 is used for enabling a cover plate 5 to be attached to the intermediate plate 6, at the moment, an injection nozzle 503 is clamped with a one-way valve 703 on the culture seat 7, so that a culture solution stored in the pressure mechanism 4 is injected into the one-way valve 703 through the injection nozzle 503 on an injection plate 501, and the culture solution flows into the culture medium 8 arranged on the culture seat 7 through a spray pipe 705 connected with the one-way valve 703 to provide nutrition for the cells cultured in the culture medium 8.
In the process of injecting liquid into the injection tray 501, the electric heating plate 502 works to heat the electric heating plate 502 and heat the heat treatment ring 601, the heated heat treatment ring 601 is insulated by the mesh plate 602, the temperature of the heat treatment ring 601 is kept, and thus the culture medium 8 in the intermediate plate 6 is in a constant temperature environment,
when needs carry out vibration treatment or rotatory observation to the cell, technical staff controls electronic guide rail 9 in class, make clamping jaw 10 carry out the displacement of vertical direction under electronic guide rail 9's effect, clamping jaw 10 removes the in-process, attract and pull culture seat 7 through the magnet on the claw frame, thereby make check valve 703 carry culture seat 7 whole along with clamping jaw 10 and move, until the chuck and the fixed disk joint of culture seat 7 bottom, culture seat 7 and perpendicular guide rail 201 separation this moment, the claw frame outage, thereby make the jack-post during operation, jack-post drive culture seat 7 rotates, it rotates to realize that culture seat 7 carries culture medium 8, realize the show of culture medium 8.
A plurality of culture mediums in this scheme are concentrated and are cultivateed together, and make things convenient for the concentrated culture of multiple cell, and also make things convenient for the show among the culture process, can realize concentrating a large amount of cells and cultivate, provide healthy growing environment for the cell of concentrating the growth, and make things convenient for the show of a plurality of cell culture mediums and concentrate the cultivation
The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.
Claims (8)
1. A composite culture tank for multicellular culture, comprising a forging die (1), characterized in that: the device is characterized in that a servo motor (101) is fixedly connected onto the bearing seat (1), a transparent cup (2) is fixedly connected onto the power output end of the servo motor (101), a top cover (3) is clamped at the top end of the transparent cup (2), a pressure mechanism (4) is fixedly connected onto the top cover (3), a cover plate (5) matched with the transparent cup (2) is fixedly connected onto the telescopic end of the pressure mechanism (4), two pairs of vertical guide rails (201) are fixedly connected onto the inner wall of the transparent cup (2), an intermediate plate (6) is arranged in the transparent cup (2), the intermediate plate (6) is fixedly connected with the vertical guide rails (201), a culture seat (7) is arranged on the lower side of the intermediate plate (6), two pairs of movable plates (701) are fixedly connected onto the culture seat (7), a plurality of culture mediums (8) are clamped onto the movable plates (701), fixedly connected with electric guide rail (9) on the outer wall of bearing seat (1), fixedly connected with clamping jaw (10) are served in the activity of electric guide rail (9), clamping jaw (10) are including the claw frame, fixedly connected with two couples and perpendicular guide rail (201) assorted cylinder electro-magnet on the claw frame, cylinder electro-magnet sliding connection is in perpendicular guide rail (201), the equal fixedly connected with in both ends and perpendicular guide rail (201) assorted gyro wheel about the cylinder electro-magnet.
2. A composite culture tank for multi-cell culture according to claim 1, characterized in that: intermediate lamella (6) are including heat treatment ring (601), the joint has mesh board (602) on the inner wall of heat treatment ring (601), two pairs of through-holes have been dug on mesh board (602), fixedly connected with heat accumulation board (603) in the through-hole, heat treatment ring (601) are made by the heat conduction material, mesh board (602) and heat accumulation board (603) are insulation material.
3. A composite culture tank for multi-cell culture according to claim 1, characterized in that: the culture medium (8) comprises a substrate (801), wherein the middle part of the substrate (801) is fixedly connected with a cultivation block (802), the distance between every two adjacent cultivation blocks (802) is 1-2cm, and the distance between the upper surface of the substrate (801) and the upper surface of each cultivation block (802) is 0.5-1 cm.
4. A composite culture tank for multi-cell culture according to claim 1, characterized in that: the upper end fixedly connected with of fly leaf (701) two pairs of hollow post (702), the top fixedly connected with of hollow post (702) and mesh board (602) assorted check valve (703), a plurality of evenly distributed's spacing ring (704) of fixedly connected with on hollow post (702), fixedly connected with spray tube (705) on spacing ring (704), spray tube (705) communicate with each other with hollow post (702), fly leaf (701) are made by the heat-insulating material.
5. A composite culture tank for multi-cell culture according to claim 1, characterized in that: the cover plate (5) comprises an injection tray (501), an electric heating plate (502) is fixedly connected to the injection tray (501), and a plurality of injection nozzles (503) matched with the one-way valves (703) are fixedly connected to the bottom end of the injection tray (501).
6. Composite culture tank for multicellular culture according to claim 1 or 5, characterized in that: pressure mechanism (4) are including storage shell (401), sliding connection has piston (402) in storage shell (401), the bottom fixedly connected with electric putter (403) of storage shell (401), the flexible end and piston (402) fixed connection of electric putter (403), the top fixedly connected with and storage shell (401) assorted electric putter (403) of piston (402), the bottom and the injection shower nozzle (503) of storage shell (401) communicate with each other.
7. A composite culture tank for multi-cell culture according to claim 1, characterized in that: two pairs of fixed disks of top fixedly connected with of servo motor (101), the bottom fixedly connected with and the fixed disk assorted chuck of fly leaf (701), bear and install in seat (1) and servo motor (101) assorted card post, the power take off end and the servo motor (101) fixed connection of card post.
8. A composite culture tank for multicellular culture according to claim 1 or 3, characterized in that: the culture medium (8) is provided with a diversion trench, and the diversion trench extends to the bottom end of the cultivation block (802).
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| CN202011442800.0A CN112553073A (en) | 2020-12-11 | 2020-12-11 | Composite culture tank for multi-cell culture |
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| CN202011442800.0A CN112553073A (en) | 2020-12-11 | 2020-12-11 | Composite culture tank for multi-cell culture |
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Cited By (1)
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| CN113549542A (en) * | 2021-07-19 | 2021-10-26 | 华北理工大学 | Microbial strain expanding culture device and method |
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Application publication date: 20210326 |