CN110622860B - Plant tissue high-efficiency culture equipment - Google Patents
Plant tissue high-efficiency culture equipment Download PDFInfo
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- CN110622860B CN110622860B CN201910960646.7A CN201910960646A CN110622860B CN 110622860 B CN110622860 B CN 110622860B CN 201910960646 A CN201910960646 A CN 201910960646A CN 110622860 B CN110622860 B CN 110622860B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention relates to the technical field of plant tissue culture equipment, and particularly discloses high-efficiency plant tissue culture equipment, wherein a micro-control flow channel is arranged in a culture solution adding micro-control core plate, a liquid outlet micro-hole group consisting of a plurality of liquid outlet micro-holes is also arranged on the lower end surface of the culture solution adding micro-control core plate corresponding to each culture placing groove, the culture solution is accurately supplemented and supplied by utilizing a micro-fluidic mode, the culture solution supplementing efficiency is improved, the output end of a culture solution supplementing controller of the invention is communicated with the liquid inlet end of the culture solution adding micro-control core plate, so that the culture solution supplementing controller controls the supplementing flow and the supplementing pressure of the supplementing culture solution, the supplementing energy and the supplementing precision of the culture solution are improved, a spiral mixing channel is also arranged on the culture solution adding micro-control core plate, thus, the different components of the culture solution can be conveniently prepared for use, prevent the mutual influence between the components after long-time preparation and mixing from influencing the culture effect.
Description
Technical Field
The invention relates to the technical field of plant tissue culture equipment, in particular to high-efficiency plant tissue culture equipment.
Background
Plant tissue culture is a biotechnological means for culturing whole plants or other valuable products under specific conditions by using cells or tissues separated from plant bodies, currently used plant tissue culture equipment is generally rapidly cultured at a certain temperature by using an incubator, and the addition amount, supplement frequency and supplement amount of a culture solution are critical in the culture process. Moreover, for some culture fluids, the nutrient effect is greatly reduced due to the interaction of the mixed components if the time course is configured. Further, when the culture medium is added, if the culture plate is pulled out, the culture tissue is adversely affected by the influence of temperature and humidity, and the efficiency and control accuracy of the culture are affected.
Based on this, the present invention aims to provide a plant tissue high-efficiency culture apparatus to solve the technical problems in the background art.
Disclosure of Invention
The present invention has been made to solve the above-mentioned problems occurring in the prior art, and an object of the present invention is to provide an apparatus for efficiently culturing plant tissue.
In order to achieve the purpose, the invention provides the following technical scheme:
a plant tissue high-efficiency culture device comprises a base and an incubator, wherein the incubator is arranged on the base, a plurality of layers of culture plates which are arranged at intervals are arranged in the incubator, a plurality of culture placing grooves for culturing plant tissues are arranged on the culture plates at intervals in an array, and the plant tissue high-efficiency culture device is characterized in that,
a culture solution adding micro-control core plate which is arranged in a close fit manner with the culture plate is also arranged above the culture plate;
a micro-control flow channel is arranged in the culture solution adding micro-control core plate, and a liquid outlet micro-hole group consisting of a plurality of liquid outlet micro-holes is further arranged on the lower end surface of the culture solution adding micro-control core plate corresponding to each culture placing groove;
still include culture solution supply controller, the output of culture solution supply controller with the feed liquor end intercommunication of culture solution addition micro-control core board, so that by culture solution supply controller control supply culture solution's supply flow and supply pressure.
Further, as preferred, the one end that the little accuse core board was added to the culture solution is provided with a plurality of supports and extends the seat, it all is provided with at least one feed liquor micropore in extending the seat to support, still be provided with the mixed channel subassembly with each feed liquor micropore intercommunication on the little accuse core board of culture solution addition, the mixed channel subassembly is through each little accuse runner and respective play liquid micropore group intercommunication, and each feed liquor micropore is connected to the culture solution supply pipe of different grade type, the other end that the little accuse core board was added to the culture solution still is provided with a plurality of supporting seats.
Further, preferably, the mixing channel assembly comprises a spiral mixing channel, a first liquid supply channel, a second liquid supply channel and a third liquid supply channel are communicated with the outermost channel of the spiral mixing channel, the innermost channel of the spiral mixing channel is communicated to the mixed liquid outlet channel, and the mixed liquid outlet channel is connected to each micro-control channel.
Preferably, the longer the micro-control flow channel communicated to the liquid outlet micro-hole group is, the larger the inner diameter of the micro-control flow channel is, and the diameter of the micro-control flow channel is less than 0.2 mm.
Further, preferably, a high-frequency oscillator is further provided in the base, and the high-frequency oscillator is capable of periodically oscillating the incubator.
Further, as a preferred option, a liquid supply main pipe is connected to the culture liquid supply controller, a plurality of liquid supply branch pipes are arranged at the end of the liquid supply main pipe, each liquid supply branch pipe is communicated with a respective liquid supply pipeline in the liquid supply main pipe so as to supply different culture liquids, and each liquid supply branch pipe is connected to a respective corresponding liquid supply micro hole in the support extension seat.
Further, preferably, the top of the incubator is also provided with a ventilation mechanism, a temperature control mechanism is arranged in the incubator, a plurality of groups of upper clamping devices and lower clamping devices are arranged on two inner side walls of the incubator, and the support extension seats and the support seats at two ends of the culture solution adding micro-control core plate are supported on the upper clamping devices and correspond to the liquid supply branch pipes; the two ends of the culture plate are clamped on the lower clamping device.
Preferably, the position of the culture plate corresponding to the culture placement groove is provided with a marking clamping groove in a one-to-one correspondence manner, and the front side of the incubator is provided with a transparent and heat-insulating sealing box door.
Further, as preferred, go up latch device and latch device structure the same down, all include the joint board, fixedly connected with joint on the lateral wall of joint board one side, and joint and locating hole are inserted and are joined in marriage, the joint groove has still been seted up to the opposite side of joint board, and the joint board passes through joint groove and layer board joint.
Further, preferably, the support extension seat of the culture solution adding micro-control core plate is connected with the upper clamping device of the incubator in a sealing manner.
Compared with the prior art, the invention has the beneficial effects that:
the micro-control flow channel is arranged in the culture solution adding micro-control core plate, the liquid outlet micro-hole group consisting of a plurality of liquid outlet micro-holes is also arranged on the lower end surface of the culture solution adding micro-control core plate corresponding to each culture placing groove, the culture solution is accurately supplemented and supplied by using a micro-control mode, the culture efficiency is improved, meanwhile, the output end of the culture solution supplementing controller of the invention is communicated with the liquid inlet end of the culture solution adding micro-control core plate, so that the culture solution supplementing controller controls the supplementing flow and the supplementing pressure of the supplemented culture solution, the supplementing energy and the supplementing precision of the culture solution are improved, the spiral mixing channel is also arranged on the culture solution adding micro-control core plate, thus, the different components of the culture solution can be prepared for use on site conveniently, the effect of culture is prevented from being influenced by the mutual influence among the components after the long-time preparation and the utilization rate of the culture is improved, the aim of rapid culture is achieved, meanwhile, the culture solution is supplemented without opening a box door, and adverse effects on tissues from the outside are prevented.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention when opened;
FIG. 2 is a schematic view of the layout structure of the culture plate and the micro-controlled core plate for adding culture solution in the incubator according to the present invention;
FIG. 3 is a schematic sectional view of the internal micro-control flow channel structure of the micro-control core plate for adding culture solution according to the present invention;
FIG. 4 is a schematic structural diagram of a spiral mixing channel according to the present invention;
FIG. 5 is a schematic view of the structure of the lower clamping device of the present invention.
Detailed Description
Referring to fig. 1 to 5, in the embodiment of the present invention, an efficient plant tissue culture apparatus includes a base 1 and an incubator 3, wherein the incubator is disposed on the base 1, a plurality of layers of culture plates 9 are disposed in the incubator 3 at intervals, a plurality of culture placement grooves 9a for culturing plant tissues are disposed in the culture plates at intervals, and a culture solution adding micro-control core plate 13 disposed in close contact with the culture plates is further disposed above the culture plates;
a micro-control flow channel 14 is arranged in the culture solution adding micro-control core plate 13, and a liquid outlet micro-hole group consisting of a plurality of liquid outlet micro-holes 15 is further arranged on the lower end surface of the culture solution adding micro-control core plate 13 corresponding to the position of each culture placing groove 9 a; still include culture solution supply controller 5, culture solution supply controller 5's output with the feed liquor end intercommunication of culture solution addition micro-controlled core board 13, so that by culture solution supply controller 5 control the supply flow and the supply pressure of supply culture solution.
Wherein, the one end that the culture solution adds micro-control core board 13 is provided with a plurality of supports and extends seat 17, it all is provided with at least one feed liquor micropore 16 in the seat to support to extend, still be provided with the mixed channel subassembly 18 with each feed liquor micropore intercommunication on the culture solution adds micro-control core board 13, mixed channel subassembly 18 is through each micro-control runner 14 and respective play liquid micropore group intercommunication, and each feed liquor micropore is connected to the culture solution supply pipe of different grade type, the other end that the culture solution adds micro-control core board 13 still is provided with a plurality of supporting seats 26.
In this embodiment, the mixing channel assembly 18 includes a spiral mixing channel 23, the outermost channel of the spiral mixing channel is communicated with a first liquid supply channel 20, a second liquid supply channel 21 and a third liquid supply channel 25, the innermost channel 24 of the spiral mixing channel is communicated with a mixing liquid outlet channel 22, and the mixing liquid outlet channel 22 is connected to each micro-control channel 14.
In a preferred embodiment, the longer the micro-control flow channel communicated to the liquid outlet micro-hole group is, the larger the inner diameter of the micro-control flow channel is, and the diameter of the micro-control flow channel is less than 0.2 mm.
Wherein, still be provided with high frequency oscillator 19 in the base, high frequency oscillator can carry out the vibration processing to the incubator regularly.
In the invention, a main liquid supply pipe 6 is connected to a culture liquid supply controller 5, a plurality of branch liquid supply pipes 11 are arranged at the end part of the main liquid supply pipe, each branch liquid supply pipe is communicated with a respective liquid supply pipeline in the main liquid supply pipe so as to supply different culture liquids, and each branch liquid supply pipe is connected to a respective corresponding liquid supply micropore 16 in the support extension seat 17.
The top of the incubator is also provided with a ventilation mechanism 12, a temperature control mechanism is arranged in the incubator, a plurality of groups of upper clamping devices 2 and lower clamping devices 8 are arranged on two inner side walls of the incubator, and support extension seats 17 and support seats 26 at two ends of the culture solution adding micro-control core plate 13 are supported on the upper clamping devices 2 and correspond to the liquid supply branch pipes 11; the two ends of the culture plate are clamped on the lower clamping device.
The position of the culture plate 9 corresponding to the culture placing groove 9a is also provided with a marking clamping groove 9b in a one-to-one correspondence manner, and the front side of the incubator is also provided with a transparent and heat-insulating sealing box door 10. Go up latch device and latch device structure the same down, all include joint board 8a, fixedly connected with joint 8b on 8a one side lateral wall of joint board, and joint 8b and locating hole 7 are inserted mutually and are joined in marriage, joint groove 8c has still been seted up to joint board 8 a's opposite side, and joint board 8a passes through joint groove 8c and layer board 9 joint.
The support extension seat 17 of the culture solution adding micro-control core plate 13 is connected with the upper clamping device 2 of the incubator in a sealing manner.
The micro-control flow channel is arranged in the culture solution adding micro-control core plate, the liquid outlet micro-hole group consisting of a plurality of liquid outlet micro-holes is also arranged on the lower end surface of the culture solution adding micro-control core plate corresponding to each culture placing groove, the culture solution is accurately supplemented and supplied by using a micro-control mode, the culture efficiency is improved, meanwhile, the output end of the culture solution supplementing controller of the invention is communicated with the liquid inlet end of the culture solution adding micro-control core plate, so that the culture solution supplementing controller controls the supplementing flow and the supplementing pressure of the supplemented culture solution, the supplementing energy and the supplementing precision of the culture solution are improved, the spiral mixing channel is also arranged on the culture solution adding micro-control core plate, thus, the different components of the culture solution can be prepared for use on site conveniently, the effect of culture is prevented from being influenced by the mutual influence among the components after the long-time preparation and the utilization rate of the culture is improved, the aim of rapid culture is achieved, meanwhile, the culture solution is supplemented without opening a box door, and adverse effects on tissues from the outside are prevented.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.
Claims (5)
1. A plant tissue high-efficiency culture device comprises a base (1) and an incubator (3), wherein the incubator is arranged on the base (1), a plurality of layers of culture plates (9) which are arranged at intervals are arranged in the incubator (3), a plurality of culture placing grooves (9a) for culturing plant tissues are arranged on the culture plates at intervals, and the plant tissue high-efficiency culture device is characterized in that,
a culture solution adding micro-control core plate (13) which is arranged in a close fit manner with the culture plate is also arranged above the culture plate;
a micro-control flow channel (14) is arranged in the culture solution adding micro-control core plate (13), and a liquid outlet micro-hole group consisting of a plurality of liquid outlet micro-holes (15) is further arranged on the lower end surface of the culture solution adding micro-control core plate (13) corresponding to the position of each culture placing groove (9 a);
the culture medium feeding device is characterized by further comprising a culture medium feeding controller (5), wherein the output end of the culture medium feeding controller (5) is communicated with the liquid inlet end of the culture medium feeding micro-control core plate (13), so that the culture medium feeding controller (5) controls the feeding flow and feeding pressure of the fed culture medium;
one end of the culture solution adding micro-control core plate (13) is provided with a plurality of supporting extension seats (17), at least one liquid inlet micropore (16) is arranged in each supporting extension seat, a mixing channel assembly (18) communicated with each liquid inlet micropore is further arranged on the culture solution adding micro-control core plate (13), each mixing channel assembly (18) is communicated with a corresponding liquid outlet micropore group through each micro-control flow channel (14), each liquid inlet micropore is connected to a culture solution supply pipe of different types, and the other end of the culture solution adding micro-control core plate (13) is further provided with a plurality of supporting seats (26); the diameter of the micro-control flow channel is less than 0.2 mm;
the mixing channel assembly (18) comprises a spiral mixing channel (23), a first liquid supply channel (20), a second liquid supply channel (21) and a third liquid supply channel (25) are communicated with the outermost channel of the spiral mixing channel, the innermost channel (24) of the spiral mixing channel is communicated with a mixed liquid outlet channel (22), and the mixed liquid outlet channel (22) is connected to each micro-control channel (14);
the longer the micro-control flow channel communicated to the liquid outlet micro-hole group is, the larger the inner diameter of the micro-control flow channel is;
a liquid supply main pipe (6) is connected to the culture liquid supply controller (5), a plurality of liquid supply branch pipes (11) are arranged at the end part of the liquid supply main pipe, each liquid supply branch pipe is communicated with a respective liquid supply pipeline in the liquid supply main pipe so as to supply different culture liquids, and each liquid supply branch pipe is connected to a liquid supply micropore (16) in the support extension seat (17) corresponding to each liquid supply branch pipe;
the position of the culture plate (9) corresponding to the culture placing groove (9a) is provided with a marking clamping groove (9b) in a one-to-one correspondence manner, and the front side of the incubator is also provided with a transparent and heat-preserving sealing box door (10).
2. The plant tissue high-efficiency culture equipment according to claim 1, wherein a high-frequency oscillator (19) is further arranged in the base, and the high-frequency oscillator can periodically carry out oscillation treatment on the culture box.
3. The plant tissue efficient culture equipment according to claim 1, wherein a ventilation mechanism (12) is further arranged at the top of the incubator, a temperature control mechanism is arranged in the incubator, a plurality of groups of upper clamping devices (2) and lower clamping devices (8) are arranged on two inner side walls of the incubator, and support extension seats (17) and support seats (26) at two ends of the culture solution adding micro-control core plate (13) are supported on the upper clamping devices (2) and correspond to the liquid supply branch pipes (11); the two ends of the culture plate are clamped on the lower clamping device.
4. The plant tissue high-efficiency cultivation equipment according to claim 3, wherein the upper clamping device and the lower clamping device are the same in structure and comprise clamping plates (8a), clamping heads (8b) are fixedly connected to the side wall of one side of each clamping plate (8a), the clamping heads (8b) are inserted into the positioning holes (7), clamping grooves (8c) are formed in the other side of each clamping plate (8a), and the clamping plates (8a) are clamped with the cultivation plate (9) through the clamping grooves (8 c).
5. The plant tissue high-efficiency culture equipment according to claim 3, wherein the support extension seat (17) of the culture solution adding micro-control core plate (13) is connected with the upper clamping device (2) of the culture box in a sealing manner.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910960646.7A CN110622860B (en) | 2019-10-10 | 2019-10-10 | Plant tissue high-efficiency culture equipment |
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| CN201910960646.7A CN110622860B (en) | 2019-10-10 | 2019-10-10 | Plant tissue high-efficiency culture equipment |
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| CN110622860B true CN110622860B (en) | 2021-04-06 |
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| CN112352676A (en) * | 2020-10-28 | 2021-02-12 | 广西壮族自治区农业科学院 | Multipoint automatic quantitative tissue culture solution stirring and filling machine |
| US11299700B1 (en) | 2021-02-19 | 2022-04-12 | Acequia Biotechnology, Llc | Bioreactor containers and methods of growing hairy roots using the same |
| CN114950585A (en) * | 2022-04-28 | 2022-08-30 | 南通大学 | Microfluidic seed culture chip and culture method thereof |
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| CN106613872A (en) * | 2016-12-27 | 2017-05-10 | 郑州吉音动漫科技有限公司 | Gardening seedling growing auxiliary culturing apparatus |
| CN206760364U (en) * | 2017-06-07 | 2017-12-19 | 合肥本盛生物科技有限公司 | A kind of incubator for tissue culture quickly bred for serrate clubmoss herb |
| CN110150144A (en) * | 2018-03-01 | 2019-08-23 | 杨国强 | A kind of plant cultivating device |
| CN109735451A (en) * | 2019-03-12 | 2019-05-10 | 邯郸学院 | A kind of plant tissue high-efficient culture device |
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