CN106047667A - Bacterial cellulose fermentation production equipment - Google Patents
Bacterial cellulose fermentation production equipment Download PDFInfo
- Publication number
- CN106047667A CN106047667A CN201610382410.6A CN201610382410A CN106047667A CN 106047667 A CN106047667 A CN 106047667A CN 201610382410 A CN201610382410 A CN 201610382410A CN 106047667 A CN106047667 A CN 106047667A
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- Prior art keywords
- cultivation box
- height
- bacterial cellulose
- baffle plate
- zymolysis equipment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides equipment for bacterial cellulose fermentation production under a standing condition. The equipment is small in occupied area, fermentation production of multiple culture boxes can be conducted simultaneously, and enough oxygen can be provided. All culture media can be added into the culture boxes through once-through operation with devices of a peristaltic pump and the like, operation can be conducted on the single culture box according to needs, and bacterial cellulose gel films with different thicknesses and quality are obtained. By means of mechanized one-time injection of the culture media, manual labor can be saved, the fermentation production and yield of the bacterial cellulose are greatly increased, and the production efficiency of the bacterial cellulose is improved finally.
Description
Invention field
The invention belongs to microbial cultivation equipment field, it particularly relates to a kind of for quiescent culture Bacterial cellulose
Fermenting and producing equipment.
Background technology
Bacterial cellulose (Bacterial cellulose is called for short BC), refers under given conditions, by some special bacterium
The general designation of the cellulose that the fermentable belonged to generates.The fermentation synthetic method of Bacterial cellulose has static fermentation and dynamic fermentation
Two kinds, wherein static fermentation refers to that antibacterial carries out fermentation culture in the gas-to-liquid contact face of the liquid culture medium stood with air and produces
Raw cellulose, Bacterial cellulose is from culture fluid and the gas-liquid interface of air, along culture fluid continuous dynamic accumulation, end form downwards
Thickness and culture fluid height is become to have positively related bacteria cellulose film.The most domestic utilize Microbe synthesis Bacterial cellulose most
Use static fermentation method.
The membranaceous Bacterial cellulose compact structure that static fermentation method is formed, the anti-intensity that rises is high, air-and water-permeable performance is good, hold
Aqueous is good, has good biocompatibility and biodegradability simultaneously, is widely used in medical science, biology, chemical industry, food
Etc. each field, such as Bone Defect Repari, cornea, wound dressing, tissue engineering bracket etc..But space required during static culture and
Manual labor is relatively big, and toxigenic capacity is high, and yield and productivity are low, and these all become restriction Bacterial cellulose industrialized production and push away
The principal element of wide application.Additionally, in the actual production process of Bacterial cellulose, it is often necessary to one time fermentation produces the most simultaneously
Going out the bacteria cellulose film of different-thickness, to meet industrial requirement, but current static fermentation equipment all fails to realize this
Effect.
Summary of the invention
The present invention provides a kind of equipment producing Bacterial cellulose at static conditions bottom fermentation, and this occupation area of equipment is little,
Multiple cultivation box fermenting and producing can be carried out simultaneously, different-thickness or the bacteria cellulose film of required thickness can be produced simultaneously,
And just all of culture medium can be joined in multiple cultivation box by once-through operation, save manual labor, be greatly improved
The fermentation yield of Bacterial cellulose and productivity, finally improve the efficiency that Bacterial cellulose produces.
The present invention provides a kind of Bacterial cellulose fermenting and producing equipment, including body and cultivation box, wherein,
Described body includes roof 1, diapire, antetheca, rear wall, left wall, You Bi；
The inner side of wherein said antetheca and rear wall is provided with multiple slide rail being parallel to each other, and the slide rail on antetheca and rear wall
Corresponding contour；In described left wall and right wall, being spaced apart highly with upper and lower two slide rails, it is arranged in sequence with direction from top to bottom
Contrary opening；
Described cultivation box has a bottom surface and four side boards, and the height of four described side boards is different, its
The height of middle top gear plate is equal with the interval height of two slide rails up and down on described body, and at the offside of top gear plate
On baffle plate, it is provided with the hole for controlling liquid culture medium height and slide block；Permissible by adjusting slider position in hole
Control to cultivate the height of the medium liquid in box, such that it is able to produce the bacteria cellulose film of different-thickness；
Distance between top gear plate and its side dams of described cultivation box, less than left wall in body with between right wall
Distance；Described cultivation carriage, on the slide rail of described body, can move left and right along slide rail.
The roof of described body is additionally provided with feed pipe and gas outlet；The diapire of described body is additionally provided with waste liquor stream
Go out pipe and air inlet.
Temperature-adjusting device, described thermostatic quantity root it is also equipped with in the antetheca or rear wall of described body
According to the big I of culture device, one or more are installed, so that homogeneous temperature in culture device, controlled.
Preferably, the height of the top gear plate of the interval height of described upper and lower two slide rails and described cultivation box is 5-15 li
Rice.
Preferably, the low 2-of the height than top gear plate that the height of other three baffle plates of described cultivation box can be the most independent
3 centimetres.
Preferably, the height of other three baffle plates of described cultivation box can be the most independent for 3-13 centimetre.
Preferably, hole and the quantity of slide block on described cultivation box baffle plate are selected from one or more, such as 2-5.
Preferably, the pore shape on described cultivation box baffle plate selects rectangle, ellipse, arch etc. commonly used in the art
Pore shape.
Preferably, around the hole on described cultivation box baffle plate, it is additionally provided with the yardstick mark of display liquid culture medium height
Note.
Preferably, outside the hole on described cultivation box baffle plate, it is additionally provided with flow guiding structure, under guiding culture fluid to flow into
Cultivate in box for one layer.
Preferably, the quantity cultivating box can placed in described Bacterial cellulose fermenting and producing equipment is 10-20.
It is thin that the Bacterial cellulose fermenting and producing equipment of the present invention can be used for carrying out that quiescent culture is large batch of, thickness is different
Fungin film, and be capable of just all of culture medium being joined in multiple cultivation box by once-through operation.
In use, first the slide block being used for controlling liquid culture medium height on each cultivation box is fixed to desired location,
Make each cultivation box can load the liquid culture medium of differing heights, such that it is able to fermenting and producing goes out difference according to producing needs
The bacteria cellulose film of thickness.Then, by cultivation carriage on the slide rail of body, it is pushed into Guan Bi, makes Bacterial cellulose fermenting and producing
Equipment integral sealing.
Then, the first floor that the most postvaccinal liquid culture medium feed pipe from equipment top is injected into equipment is cultivated
Inside box, when the culture medium liquid level during the first floor cultivates box reaches to cultivate the liquid level set by box top shoe, liquid
Culture medium (or the flow guiding structure outside hole) can be flowed into next layer and cultivates in box the hole above slide block.With this type of
Pushing away, along with the continuous injection of liquid culture medium, culture medium can cultivate box all of cultivation box that fills up step by step from the first floor, now
Each cultivates the culture medium height in box is the height arranged beforehand through slide block, then carries out fermentation culture.
After fermentation culture terminates, can pull out as required from the left and right sides of Bacterial cellulose fermenting and producing equipment
The cultivation box of any one layer, carries out the collection of Bacterial cellulose and is carried out cultivating box.
Present configuration is simple, easy to use, floor space is little, can carry out the fermentation of multiple cultivation box simultaneously, can give birth to simultaneously
Output different-thickness or the bacteria cellulose film of required thickness, and just all of culture medium can be added by once-through operation
Enter in multiple cultivation boxes, save manual labor, be greatly improved fermentation yield and the productivity of Bacterial cellulose.
Accompanying drawing explanation
The structural representation of the Bacterial cellulose fermenting and producing apparatus body of Fig. 1: the present invention.
The Bacterial cellulose fermenting and producing equipment of Fig. 2: the present invention cultivates the structural representation of box.
The front section view of the Bacterial cellulose fermenting and producing equipment of Fig. 3: the present invention.
Detailed description of the invention
The present invention is described in more detail for Structure Figure and embodiment below, artisan will appreciate that
Without departing from the change made in the case of scope and spirit of the present invention, belong to the scope of the present invention.
Embodiment 1:
The present embodiment is a kind of preferred structure of the Bacterial cellulose fermenting and producing equipment of the present invention.Such as Fig. 1 and Fig. 2 institute
Showing, the Bacterial cellulose fermenting and producing equipment of the present embodiment includes body and cultivates box, wherein,
Described body includes roof 1, diapire, antetheca 2, rear wall, left wall, right wall 3；Wherein said antetheca and the inner side of rear wall
It is provided with multiple slide rail 4 being parallel to each other, and antetheca is corresponding with the slide rail on rear wall contour；In described left wall and right wall, with
Being spaced apart highly of upper and lower two slide rails, is arranged in sequence with opening 5 in opposite direction from top to bottom；
The roof of described body is additionally provided with feed pipe 6 and gas outlet 7；
The diapire of described body is additionally provided with waste liquid effuser 8 and air inlet 9.
Described cultivation box has a bottom surface and four side boards, and the height of described four side boards is different, wherein
The height of top gear plate 10 is equal with the interval height of two slide rails 4 up and down on body, is highly 10 centimetres, other three
Height of baffle plate is 8 centimetres, on the side dams 11 of top gear plate 10, is provided with a vertical hole 12, is provided with use in hole
Slide block 13 in regulation liquid culture medium height；This slide block can control to cultivate the height of the medium liquid in box, thus can
To produce the bacteria cellulose film of different-thickness, around the hole on described cultivation box baffle plate, it is additionally provided with display liquid and cultivates
The scale designation 14 of base height；
In the present embodiment, the distance between top gear plate 10 and its side dams 11 of described cultivation box is 25 centimetres,
In body, the distance between left wall and right wall is 28 centimetres；Described cultivation carriage, can be along cunning on the slide rail 4 of described body
Rail 4 moves left and right.
Embodiment 2:
The present embodiment is the another kind of preferred structure of the Bacterial cellulose fermenting and producing equipment of the present invention.Such as Fig. 1 and Fig. 3 institute
Showing, the Bacterial cellulose fermenting and producing equipment of the present embodiment includes body and cultivates the knot of box, wherein said body and embodiment 1
Structure is identical.
Described cultivation box has a bottom surface and four side boards, and the height of described four side boards is different, wherein
The height of top gear plate 10 is equal with the interval height of two slide rails 4 up and down on body, is highly 12 centimetres, other three
Height of baffle plate is 9 centimetres, on the side dams 11 of top gear plate 10, is provided with two vertical holes 12, one of them hole
Being positioned at baffle plate top, another hole is positioned at baffle plate bottom, is designed with for regulating liquid culture medium height in each hole
Slide block 13；By arranging the two slide block position in two holes, it is possible to arrange more different height, such that it is able to
Produce the bacteria cellulose film of more different-thickness, around the hole on described cultivation box baffle plate, be additionally provided with display liquid training
Support the scale designation 14 of base height.
In the present embodiment, the distance between top gear plate 10 and its side dams 11 of described cultivation box is 20 centimetres,
In body, the distance between left wall and right wall is 24 centimetres；Described cultivation carriage, can be along slide rail on the slide rail 4 of described body
4 move left and right.
Embodiment 3:
The present embodiment is the Bacterial cellulose fermenting and producing apparatus body of present invention structure together with cultivating capsule
Example.As it is shown on figure 3, the Bacterial cellulose fermenting and producing equipment of the present embodiment there are 10 cultivate box, wherein 5 cultivations above
Box be open mode place, below 5 cultivate boxes be closed mode place, i.e. carry out state during fermentation culture.Fig. 3 cultivates in box
The fluid medium that dark matter is different-thickness 15.
When the Bacterial cellulose fermenting and producing equipment using the present invention carries out the production of quiescence cells cellulose fermentation, first will
On each cultivation box, the slide block 13 for regulating liquid culture medium height is fixed to desired location, makes each cultivation box can basis
Produce and need, load the liquid culture medium of differing heights, such that it is able to fermenting and producing goes out the bacteria cellulose film of different-thickness.So
After by cultivate carriage on the slide rail of body, be pushed into Guan Bi, make Bacterial cellulose fermenting and producing equipment integral sealing.
Then, by the most postvaccinal liquid culture medium entering from the Bacterial cellulose fermenting and producing equipment top of the present invention
The first floor that material pipe 6 is injected in equipment is cultivated inside box, and the culture medium liquid level in the first floor cultivates box reaches to cultivate on box
During liquid level set by slide block 13, fluid medium can be flowed into next layer the hole 12 above slide block and cultivate box
In.By that analogy, along with the continuous injection of liquid culture medium, culture medium can from the first floor cultivate box step by step fill up all of training
Supporting box, the culture medium height during now each cultivates box is the differing heights arranged beforehand through slide block, then carries out fermentation training
Support.
After fermentation culture terminates, can pull out as required from the left and right sides of Bacterial cellulose fermenting and producing equipment
The cultivation box of any one layer, carries out the collection of Bacterial cellulose and is carried out cultivating box.
Claims (9)
1. a Zymolysis Equipment, including body and at least 2 cultivation boxes, wherein,
Described body includes roof (1), diapire, antetheca (2), rear wall, left wall, You Bi (3)；
The inner side of wherein said antetheca (2) and rear wall is provided with multiple slide rail (4) being parallel to each other, and on antetheca (2) and rear wall
Slide rail (4) corresponding contour；In described left wall and You Bi (3), it is spaced apart height, from upper with upper and lower two slide rails (4)
Under be arranged in sequence with opening (5) in opposite direction；
Described cultivation box has a bottom surface and four side boards, and described, wherein the height of side board baffle plate (10) is less than
Or the interval height equal to two slide rails (4) up and down on described body, and be provided with opening (5) side side dams
(11) on, it is provided with the structure for controlling liquid culture medium height, is preferably provided with hole (12) and slide block (13)；
Described cultivation carriage, on the slide rail (4) of described body, can move left and right along slide rail (4), and the most described cultivation box can
Dismantle from opening part.
Zymolysis Equipment the most according to claim 1, is additionally provided with feed pipe (6) on the roof (1) of described body and gives vent to anger
Mouth (7)；The diapire of described body is additionally provided with waste liquid effuser (8) and air inlet (9)；Preferably at the antetheca of described body
(2) or be also equipped with temperature-adjusting device in rear wall, furthermore it is preferred that on the side dams being provided with opening (5) side cultivating box
It is provided with handle.
Zymolysis Equipment the most according to claim 5, the height of the baffle plate of wherein said cultivation box can be the most independent for 5-
15 centimetres.
Zymolysis Equipment the most according to claim 1, hole (12) on wherein said cultivation box baffle plate and slide block (13)
Any one in 1-5 group of quantity.
Zymolysis Equipment the most according to claim 4, hole (12) shape on wherein said cultivation box baffle plate selects rectangular
Shape, ellipse, arch.
Zymolysis Equipment the most according to claim 4, wherein around the hole (12) on described cultivation box baffle plate, is additionally provided with
The scale designation (14) of display liquid culture medium height.
Zymolysis Equipment the most according to claim 1, wherein the outside of the hole (12) on described cultivation box baffle plate, is additionally provided with
Flow guiding structure.
8. according to the Zymolysis Equipment according to any one of claim 1-7, in wherein said Bacterial cellulose fermenting and producing equipment
Cultivation box quantity be 3-100, preferably 5-60, more preferably 10-40.
9., according to the Zymolysis Equipment according to any one of claim 1-8, for static fermentation, it is preferred for preparing bacterial fibers
Element.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610382410.6A CN106047667A (en) | 2016-05-30 | 2016-05-30 | Bacterial cellulose fermentation production equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610382410.6A CN106047667A (en) | 2016-05-30 | 2016-05-30 | Bacterial cellulose fermentation production equipment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106047667A true CN106047667A (en) | 2016-10-26 |
Family
ID=57171864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610382410.6A Pending CN106047667A (en) | 2016-05-30 | 2016-05-30 | Bacterial cellulose fermentation production equipment |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105950682A (en) * | 2016-05-30 | 2016-09-21 | 天津科技大学 | Fermentation method |
| CN107446796A (en) * | 2016-05-30 | 2017-12-08 | 天津科技大学 | A kind of bacteria cellulose fermenting and producing equipment |
| CN109161473A (en) * | 2018-09-27 | 2019-01-08 | 北京观澜科技有限公司 | A kind of the fermented and cultured device and cultural method of bacteria cellulose |
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| CN107446796A (en) * | 2016-05-30 | 2017-12-08 | 天津科技大学 | A kind of bacteria cellulose fermenting and producing equipment |
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| CN109161473A (en) * | 2018-09-27 | 2019-01-08 | 北京观澜科技有限公司 | A kind of the fermented and cultured device and cultural method of bacteria cellulose |
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