CN107446796B - Bacterial cellulose fermentation production equipment - Google Patents
Bacterial cellulose fermentation production equipment Download PDFInfo
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- CN107446796B CN107446796B CN201610367656.6A CN201610367656A CN107446796B CN 107446796 B CN107446796 B CN 107446796B CN 201610367656 A CN201610367656 A CN 201610367656A CN 107446796 B CN107446796 B CN 107446796B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
Abstract
The invention provides bacterial cellulose fermentation production equipment which comprises a body and a culture box and is used for static culture of bacterial cellulose. The bacterial cellulose fermentation production equipment is small in occupied area, can simultaneously perform fermentation production of a plurality of culture boxes, can simultaneously produce bacterial cellulose membranes with different thicknesses or required thicknesses, can add all culture mediums into the culture boxes through one-time operation, saves labor, greatly improves the fermentation yield and the yield of the bacterial cellulose, and finally improves the efficiency of the bacterial cellulose production.
Description
Technical Field
The invention belongs to the field of microbial culture equipment, and particularly relates to fermentation production equipment for static culture of bacterial cellulose.
Background
Bacterial Cellulose (BC) is a generic term for cellulose produced by fermentation of microorganisms of certain specific genera under specific conditions. The fermentation synthesis method of the bacterial cellulose comprises static fermentation and dynamic fermentation, wherein the static fermentation refers to the fermentation culture of bacteria on the gas-liquid contact surface of a static liquid culture medium and air to generate the cellulose, the bacterial cellulose is continuously synthesized and accumulated downwards along the culture solution from the gas-liquid interface of the culture solution and the air, and finally the bacterial cellulose film with the thickness positively correlated to the height of the culture solution is formed. At present, the static fermentation method is mostly adopted for synthesizing bacterial cellulose by using microorganisms in China.
The membrane-shaped bacterial cellulose formed by the static fermentation method has a compact structure, high expansion strength, good air and water permeability, good water retention property, good biocompatibility and biodegradability, and is widely applied to various fields of medicine, biology, chemical industry, food and the like, such as bone repair, cornea, wound dressing, tissue engineering scaffolds and the like. However, the space and labor required in the static culture process are large, the culture cost is high, and the yield and the productivity are low, which all become main factors restricting the industrial production, popularization and application of the bacterial cellulose. In addition, in the actual production process of bacterial cellulose, bacterial cellulose membranes with different thicknesses are often produced simultaneously by one-time fermentation to meet the industrial requirements, but the current static fermentation equipment cannot achieve the effect.
Disclosure of Invention
The invention provides equipment for producing bacterial cellulose by fermentation under the standing condition, which has small floor area, can simultaneously carry out fermentation production of a plurality of culture boxes, can simultaneously produce bacterial cellulose membranes with different thicknesses or required thicknesses, can add all culture media into the plurality of culture boxes by one-time operation, saves labor force, greatly improves the fermentation yield and the yield of the bacterial cellulose, and finally improves the production efficiency of the bacterial cellulose.
The invention provides bacterial cellulose fermentation production equipment, which comprises a body and a culture box, wherein,
the body comprises a top wall 1, a bottom wall, a front wall, a rear wall, a left wall and a right wall;
the inner sides of the front wall and the rear wall are provided with a plurality of mutually parallel slide rails, and the slide rails on the front wall and the rear wall are correspondingly equal in height; openings in opposite directions are sequentially arranged on the left wall and the right wall from top to bottom by taking the interval between the upper sliding rail and the lower sliding rail as the height;
the culture box is provided with a bottom surface and four side baffles, the heights of the four side baffles are different, the height of the highest baffle is equal to the interval height of the upper slide rail and the lower slide rail on the body, and a pore and a sliding block for controlling the height of the liquid culture medium are arranged on the opposite side baffle of the highest baffle; the height of the culture medium liquid in the culture box can be controlled by adjusting the position of the slide block in the pore, so that bacterial cellulose membranes with different thicknesses can be produced;
the distance between the highest baffle of the culture box and the baffle on the opposite side of the highest baffle is smaller than the distance between the left wall and the right wall in the body; the culture box frame is arranged on the slide rail of the body and can move left and right along the slide rail.
The top wall of the body is also provided with a feeding pipe and an air outlet; and a waste liquid outlet pipe and an air inlet are also arranged on the bottom wall of the body.
The front wall or the rear wall of the body is also internally provided with a temperature adjusting device, and the number of the temperature adjusting devices can be 1 or more according to the size of the culture equipment, so that the temperature in the culture equipment is uniform and controllable.
Preferably, the spacing height of the upper slide rail and the lower slide rail and the height of the highest baffle of the culture box are 5-15 cm.
Preferably, the heights of the other three baffles of the culture box can be respectively and independently 2-3 cm lower than the height of the highest baffle.
Preferably, the heights of the other three baffles of the culture box can be 3-13 cm independently.
Preferably, the number of the apertures and the sliders on the baffle plate of the culture box is selected from one or more, for example, 2 to 5.
Preferably, the shape of the pore on the baffle plate of the culture box is selected from rectangular, oval, arched and other pore shapes commonly used in the field.
Preferably, a scale mark for displaying the height of the liquid culture medium is arranged around the aperture on the baffle plate of the culture box.
Preferably, a drainage structure is arranged outside the hole on the baffle plate of the culture box to guide the culture solution to flow into the culture box of the next layer.
Preferably, the number of the culture boxes which can be placed in the bacterial cellulose fermentation production equipment is 10-20.
The bacterial cellulose fermentation production equipment can be used for carrying out static culture on a large number of bacterial cellulose membranes with different thicknesses, and can realize that all culture mediums are added into a plurality of culture boxes through one-time operation.
When the device is used, the slide block used for controlling the height of the liquid culture medium on each culture box is fixed to a required position, so that the liquid culture media with different heights can be loaded on each culture box according to production requirements, and bacterial cellulose membranes with different thicknesses can be produced through fermentation. Then the culture box frame is arranged on the slide rail of the body and pushed to be closed, so that the bacterial cellulose fermentation production equipment is integrally sealed.
Then, the liquid culture medium inoculated in advance is injected into the first layer of culture box in the equipment from a feed pipe at the top of the equipment, and when the liquid level height of the culture medium in the first layer of culture box reaches the liquid level height set by the slide block on the culture box, the liquid culture medium can flow into the next layer of culture box from the hole on the slide block (or through a drainage structure outside the hole). By analogy, with the continuous injection of the liquid culture medium, the culture medium can fill all the culture boxes step by step from the first-layer culture box, and the height of the culture medium in each culture box is the height preset by the sliding block, and then fermentation culture is carried out.
After the fermentation culture is finished, the culture boxes of any layer can be pulled out from the left side and the right side of the bacterial cellulose fermentation production equipment as required, and the collection of bacterial cellulose and the cleaning of the culture boxes are carried out.
The invention has simple structure, convenient use and small occupied area, can simultaneously ferment a plurality of culture boxes, can simultaneously produce bacterial cellulose membranes with different thicknesses or required thicknesses, can add all culture media into the plurality of culture boxes through one-time operation, saves labor and greatly improves the fermentation yield and the productivity of the bacterial cellulose.
Drawings
FIG. 1: the invention discloses a structural schematic diagram of a bacterial cellulose fermentation production equipment body.
FIG. 2: the invention discloses a structural schematic diagram of a culture box of bacterial cellulose fermentation production equipment.
FIG. 3: the invention also discloses another structural schematic diagram of the culture box of the bacterial cellulose fermentation production equipment.
FIG. 4: the invention relates to a front cross-sectional view of bacterial cellulose fermentation production equipment.
Detailed Description
The present invention is further described in detail with reference to the following structural drawings and examples, and it will be appreciated by those skilled in the art that changes may be made without departing from the scope and spirit of the invention.
Example 1:
this embodiment is a preferred structure of the bacterial cellulose fermentation production apparatus of the present invention. As shown in fig. 1 and 2, the bacterial cellulose fermentation production apparatus of the present embodiment includes a body and a culture box, wherein,
the body comprises a top wall 1, a bottom wall, a front wall 2, a rear wall, a left wall and a right wall 3; wherein, the inner sides of the front wall and the rear wall are provided with a plurality of mutually parallel slide rails 4, and the slide rails on the front wall and the rear wall are correspondingly equal in height; openings 5 in opposite directions are sequentially arranged on the left wall and the right wall from top to bottom by taking the interval between the upper sliding rail and the lower sliding rail as the height;
a feed pipe 6 and an air outlet 7 are also arranged on the top wall of the body;
a waste liquid outlet pipe 8 and an air inlet 9 are also arranged on the bottom wall of the body.
The culture box is provided with a bottom surface and four side baffles, the heights of the four side baffles are different, the height of the highest baffle 10 is equal to the interval height of the upper slide rail 4 and the lower slide rail 4 on the body, the heights of the highest baffle are all 10 cm, the heights of the other three baffles are all 8 cm, a vertical hole 12 is formed in the opposite side baffle 11 of the highest baffle 10, and a sliding block 13 for adjusting the height of a liquid culture medium is arranged in the hole; the slide block can control the height of the culture medium liquid in the culture box, so that bacterial cellulose membranes with different thicknesses can be produced, and scale marks 14 for displaying the height of the liquid culture medium are arranged around the holes on the baffle plate of the culture box;
in the embodiment, the distance between the highest baffle plate 10 and the opposite side baffle plate 11 of the culture box is 25 cm, and the distance between the left wall and the right wall in the body is 28 cm; the culture box is arranged on the slide rail 4 of the body and can move left and right along the slide rail 4.
Example 2:
this embodiment is another preferred structure of the bacterial cellulose fermentation production apparatus of the present invention. As shown in FIGS. 1 and 3, the bacterial cellulose fermentation production apparatus of the present embodiment includes a body and a culture box, wherein the body has the same structure as that of embodiment 1.
The culture box is provided with a bottom surface and four side baffles, the heights of the four side baffles are different, the height of the highest baffle 10 is equal to the interval height of the upper slide rail 4 and the lower slide rail 4 on the body, the heights of the highest baffle are all 12 cm, the heights of the other three baffles are all 9 cm, two vertical holes 12 are formed in the opposite side baffle 11 of the highest baffle 10, one hole is located at the upper part of the baffle, the other hole is located at the lower part of the baffle, and a sliding block 13 for adjusting the height of a liquid culture medium is arranged in each hole; by arranging the two sliders at the positions in the two holes, more different heights can be arranged, so that more bacterial cellulose membranes with different thicknesses can be produced, and the scale marks 14 for displaying the height of the liquid culture medium are arranged around the holes on the baffle plate of the culture box.
In the embodiment, the distance between the highest baffle plate 10 and the opposite side baffle plate 11 of the culture box is 20 cm, and the distance between the left wall and the right wall in the body is 24 cm; the culture box is arranged on the slide rail 4 of the body and can move left and right along the slide rail 4.
Example 3:
this embodiment is an example of the structure in which the bacterial cellulose fermentation production apparatus body and the culture box of the present invention are assembled together. As shown in FIG. 3, the bacterial cellulose fermentation production apparatus of the present embodiment has 10 cassettes, wherein the upper 5 cassettes are placed in an open state, and the lower 5 cassettes are placed in a closed state, i.e., a state in which fermentation culture is performed. The dark material in the cassette of FIG. 3 is liquid medium 15 of varying thickness.
When the bacterial cellulose fermentation production equipment is used for carrying out static cell cellulose fermentation production, the slide block 13 used for adjusting the height of the liquid culture medium on each culture box is fixed at a required position, so that the liquid culture mediums with different heights can be loaded on each culture box according to production requirements, and bacterial cellulose membranes with different thicknesses can be produced through fermentation. Then the culture box frame is arranged on the slide rail of the body and pushed to be closed, so that the bacterial cellulose fermentation production equipment is integrally sealed.
Then, the liquid culture medium inoculated in advance is injected into the first layer of culture box in the equipment from the feeding pipe 6 at the top of the bacterial cellulose fermentation production equipment, and when the liquid level height of the culture medium in the first layer of culture box reaches the liquid level height set by the upper sliding block 13 of the culture box, the liquid culture medium flows into the next layer of culture box from the hole 12 on the upper surface of the sliding block. By analogy, along with the continuous injection of the liquid culture medium, the culture medium can fill all the culture boxes step by step from the first-layer culture box, the culture medium height in each culture box is different heights which are preset through the sliding block, and then fermentation culture is carried out.
After the fermentation culture is finished, the culture boxes of any layer can be pulled out from the left side and the right side of the bacterial cellulose fermentation production equipment as required, and the collection of bacterial cellulose and the cleaning of the culture boxes are carried out.
Claims (8)
1. A fermentation device of bacterial cellulose membrane comprises a body and at least 2 culture boxes, wherein,
the body comprises a top wall (1), a bottom wall, a front wall (2), a rear wall, a left wall and a right wall (3);
wherein the inner sides of the front wall (2) and the rear wall are provided with a plurality of mutually parallel slide rails (4), and the slide rails (4) on the front wall (2) and the rear wall are correspondingly equal in height; openings (5) with opposite directions are sequentially arranged on the left wall and the right wall (3) from top to bottom by taking the interval between the upper sliding rail and the lower sliding rail (4) as the height;
the culture box is provided with a bottom surface and four side baffles, wherein the height of the side baffles (10) is less than or equal to the spacing height of an upper slide rail (4) and a lower slide rail (4) on the body, and a structure for controlling the height of the liquid culture medium, namely a pore (12) and a slide block (13), is arranged on the opposite side baffle (11) on the side provided with the opening (5); a scale mark (14) for displaying the height of the liquid culture medium is arranged around the aperture (12) on the baffle plate of the culture box; a drainage structure is arranged outside the hole (12) on the baffle plate of the culture box;
the culture box is erected on a sliding rail (4) of the body and can move left and right along the sliding rail (4), and the culture box can be detached from the opening; wherein the content of the first and second substances,
a feed pipe (6) and an air outlet (7) are also arranged on the top wall (1) of the body; a waste liquid outlet pipe (8) and an air inlet (9) are also arranged on the bottom wall of the body; namely, a temperature adjusting device is also arranged in the front wall (2) or the rear wall of the body, and a handle is arranged on the side opposite to the side of the baffle plate provided with the opening (5) of the culture box;
when the fermentation equipment is used, firstly, a sliding block used for controlling the height of the liquid culture medium on each culture box is fixed to a required position, so that each culture box can be loaded with the liquid culture medium with different heights according to production requirements, and bacterial cellulose membranes with different thicknesses are produced through fermentation; then putting the culture box frame on the slide rail of the body, pushing the culture box frame to be closed, and sealing the bacterial cellulose fermentation production equipment integrally; then, liquid culture medium inoculated in advance is injected into a first layer of culture box in the fermentation equipment from a feed pipe at the top of the fermentation equipment, and when the liquid level height of the culture medium in the first layer of culture box reaches the liquid level height set by a sliding block on the culture box, the liquid culture medium flows into a next layer of culture box from a hole on the sliding block or a drainage structure outside the hole; with the continuous injection of the liquid culture medium, the culture medium can fill all the culture boxes step by step from the first-layer culture box, the height of the culture medium in each culture box is the height preset by the sliding block, and then fermentation culture is carried out; after the fermentation culture is finished, the culture boxes of any layer are drawn out from the left side and the right side of the bacterial cellulose fermentation production equipment as required, and the bacterial cellulose is collected and the culture boxes are cleaned.
2. The fermentation apparatus according to claim 1, wherein the height of the baffle plates of the culture cassette is each independently 5 to 15 cm.
3. The fermentation apparatus according to claim 1, wherein the number of the apertures (12) and the sliders (13) on the baffle plate of the culture cassette is selected from any one of the groups 1 to 5.
4. A fermentation apparatus according to claim 3, wherein the aperture (12) in the baffle plate is selected from the group consisting of rectangular, oval and arcuate.
5. The fermentation apparatus according to any one of claims 1 to 4, wherein the number of the culture boxes is 3 to 100.
6. The fermentation apparatus according to claim 5, wherein the number of the culture boxes is 5 to 60.
7. The fermentation apparatus according to claim 6, wherein the number of the culture boxes is 10 to 40.
8. Fermentation apparatus according to claim 1, wherein the fermentation apparatus comprises a static fermentation for the preparation of bacterial cellulose.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610367656.6A CN107446796B (en) | 2016-05-30 | 2016-05-30 | Bacterial cellulose fermentation production equipment |
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| CN201610367656.6A CN107446796B (en) | 2016-05-30 | 2016-05-30 | Bacterial cellulose fermentation production equipment |
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| CN105950682A (en) * | 2016-05-30 | 2016-09-21 | 天津科技大学 | Fermentation method |
| CN106047667A (en) * | 2016-05-30 | 2016-10-26 | 天津科技大学 | Bacterial cellulose fermentation production equipment |
| CN113897404A (en) * | 2021-10-26 | 2022-01-07 | 山东纳美德生物科技有限公司 | Production device capable of precisely controlling thickness of bacterial cellulose membrane and preparation method thereof |
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