CN102373151B - Bioreactor and photo bio culture systems - Google Patents
Bioreactor and photo bio culture systems Download PDFInfo
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- CN102373151B CN102373151B CN201010266749.2A CN201010266749A CN102373151B CN 102373151 B CN102373151 B CN 102373151B CN 201010266749 A CN201010266749 A CN 201010266749A CN 102373151 B CN102373151 B CN 102373151B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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Abstract
The invention discloses a kind of pocket type bioreactor be made up of fexible film and the photo bio culture systems comprising this bioreactor.Described bioreactor is formed with the liquid discharge entrance for injecting and get rid of photo bio nutrient solution, and is provided with aerating apparatus in described bioreactor.Multiple isolating points is formed in described bioreactor, for the photo bio nutrient solution in bioreactor is divided into multiple liquid regions be interconnected, described aerating apparatus is from the bottom of bioreactor to jet flow stream in photo bio nutrient solution, and described gas comprises the reactant gases needed for photo bio growth.In the present invention, the gas that aerating apparatus ejects can flow through each liquid regions from bottom to up swimmingly, this can not only make the photo bio in photo bio nutrient solution and reactant gases fully react, and photo bio nutrient solution can be made to be stirred fully by gas, thus improve photo bio by optical efficiency.
Description
Technical field
The present invention relates to photo bio culture technique field, bioreactor of espespecially a kind of film type and preparation method thereof, photo bio culture systems and method.
Background technology
At present, microalgae industry is produced and is still generally adopted open pool culture systems, and the advantage of this mode is less investment, easy and simple to handle, be easy to produce, but there is following deficiency: (1) is easily affected by the external environment, and is difficult to the temperature that keeps comparatively being suitable for and illumination; (2) pollution of dust, insect and miscellaneous bacteria can be subject to, not easily keep high-quality single algae to cultivate; (3) moisture easily evaporates; (4) illuminating area is low with the ratio of volume of culture, luminous energy and CO2 utilization ratio not high, cannot high-density culture be realized; (5) objectionable impurities such as heavy metal is easy to algae cylinder accumulation; (6) algae liquid concentration is general lower, so that results costly; (7) floor space is large.The high cost causing micro-algae to produce for above-mentioned reasons is with inefficient.
Therefore, comparatively efficient and be easy to the development trend that closed photo thing reactive system that working condition controls just becomes both culturing microalgae industry.The more closed photo bioreactor of current application has sealed pipe type reactor, plate-type reactor and film type reactor etc.
As shown in Figure 1, the two dimensional structure schematic diagram of film type reactor common in prior art is.Its entirety is a kind of transparent film pocket type structure, is to use more a kind of plastic bag type bio-light reactor both at home and abroad at present.Material is transparent plastics film, and in figure, A is fluid inlet, and B is liquid outlet.Culturing process is that nutritive medium injects from fluid inlet, relies on gravity to decline successively, discharges from liquid outlet.
The film type reactor of prior art adopts sealed structure, and cleaning is inconvenient, and it needs circulating liquid in the process of cultivating micro-algae, and in order to ensure that liquid ceaselessly flows, need power-equipment incessantly nutrient solution to be sent to water filling port, power consumption is larger.And there is the characteristic distributions of stacked on top of one another due to its nutrient solution, due to the isolation between each layer, the aerator being arranged on bottom can not well for culture supernatants provides reactant gases, and its aeration effect is bad.The same with tubular reactor, aerating gas and nutrient solution are easy to occur layering, cause the utilization ratio occurring CO2 not high, and oxygen is difficult to be separated, and is unfavorable for that micro-algae produces; And isolate because this bioreactor has horizontal layering, micro-algae can deposit on lateral isolation band, brings difficulty to the cleaning of microalgae harvesting and bioreactor.
Summary of the invention
The embodiment of the present invention provides a kind of bioreactor and preparation method thereof, photo bio culture systems and method, has that the power consumption of film type reactor is large, the problem of aeration poor effect in order to solve in prior art.
According to an aspect of the present invention, a kind of pocket type bioreactor be made up of fexible film is provided, described bioreactor is formed with the liquid discharge entrance for injecting and get rid of photo bio nutrient solution, and is provided with aerating apparatus in described bioreactor.Wherein, multiple isolating points is formed in described bioreactor, for the photo bio nutrient solution in bioreactor is divided into multiple liquid regions be interconnected, described aerating apparatus is from the bottom of described bioreactor to jet flow stream in photo bio nutrient solution, and described gas comprises the reactant gases needed for photo bio growth.
According to a preferred embodiment of the present invention, front side fexible film and rear side fexible film by making bioreactor are heat sealing at mutually comes together to form described multiple isolating points.
According to a further advantageous embodiment of the invention, front side fexible film and rear side fexible film by making bioreactor are mutually clamped together and form described multiple isolating points.
According to a further advantageous embodiment of the invention, described bioreactor also comprises the both sides, front and back that are arranged in described bioreactor and relative at least one pair of column in front and back, for the sandwich fexible film from described bioreactor, described each column has the projection for the formation of described multiple isolating points.
According to a further advantageous embodiment of the invention, the fore-and-aft clearance between the projection of described every column is 0 ~ 1mm.
According to a further advantageous embodiment of the invention, described bioreactor is made up of two panels fexible film, the mode that the lower edge of described two panels fexible film and both sides of the edge are clamped by clips or sealed by the mode of heat seal, when adopting the mode of clips clamping, the clips of the lower edge that described two panels fexible film is clamped by clips and both sides of the edge is opened when gathering in the crops or clean.
According to a further advantageous embodiment of the invention, described bioreactor is made up of a slice fexible film, described a slice fexible film is folded into two-layer, and the mode that the open sides edge formed after folding and/or lower edge are clamped by clips or sealed by the mode of heat seal, when adopting the mode of clips clamping, the clips of the lateral edges that described a slice fexible film is clamped by clips and/or lower edge is opened when gathering in the crops or clean.
According to a further advantageous embodiment of the invention, described bioreactor is made up of integral type tubular fexible film, the mode that the lower edge of described tubular fexible film is clamped by clips or sealed by the mode of heat seal, when adopting the mode of clips clamping, the clips of the lower edge that described tubular fexible film is clamped by clips is opened when gathering in the crops or clean.
According to a further advantageous embodiment of the invention, the top of described bioreactor is provided with the sprung parts hung.
According to a further advantageous embodiment of the invention, described sprung parts comprises: the suspension element that suppending hole, hanger bar or hanger bar and suspension line form.
According to a further advantageous embodiment of the invention, described fexible film selects the film of llowing group of materials: polyethylene PE film, polyvinylchloride film or polyurethane PU film.
According to another aspect of the present invention, a kind of photo bio culture systems is provided, comprises: aforesaid bioreactor; And the transport pipe to be connected with the liquid discharge entrance of described bioreactor, for injecting photo bio nutrient solution and/or collect the photo bio nutrient solution of described bioreactor in described bioreactor.
Compared with prior art, owing to being formed with multiple isolating points in pocket type bioreactor, the photo bio nutrient solution in bioreactor is divided into multiple liquid regions be interconnected.Therefore, the gas that aerating apparatus ejects can flow through each liquid regions from bottom to up swimmingly, this can not only make the photo bio in photo bio nutrient solution and reactant gases fully react, and photo bio nutrient solution can be made to be stirred fully by gas, thus improve photo bio by optical efficiency.In addition, when adopting clamping mode to form pocket type bioreactor, whole pocket type bioreactor can be opened easily, cleaning and gathering in the crops all very convenient.
Accompanying drawing explanation
Fig. 1 is the two dimensional structure schematic diagram of film type reactor in prior art;
Fig. 2 is the effect schematic diagram of point-like isolation in the embodiment of the present invention;
Fig. 3 is the structural representation of bioreactor in the embodiment of the present invention one;
Fig. 4 is the structural representation of bioreactor in the embodiment of the present invention two;
Fig. 5 is the structural representation of bioreactor in the embodiment of the present invention three;
Fig. 6 is the structural representation of bioreactor in the embodiment of the present invention four;
Fig. 7 is the structural representation of photo bio culture systems in the embodiment of the present invention;
Fig. 8 is the clamp structure schematic diagram of the multiple isolating points forming bioreactor of the present invention.
Embodiment
In order to overcome in prior art, film type reactor existence cleaning is difficult, power consumption is large, the problem of aeration poor effect, and the embodiment of the present invention provides a kind of bioreactor of film type, and this reactor comprises: at least a slice fexible film and aerating apparatus.
Wherein, at least a slice fexible film forms the bag-like container (bag-shaped bioreactor) with fluid continuity; Between the fexible film of the inner both sides of bag-like container, multiple isolating points is set, photo bio (such as micro-algae) nutrient solution is divided into some liquid regions be interconnected; Bag-like container top is provided with the sprung parts for hanging bioreactor, and bottom is provided with the liquid discharge entrance for injecting and get rid of photo bio nutrient solution.
Aerating apparatus is arranged on the bottom of the liquid regions in bioreactor, for jet flow stream in photo bio nutrient solution, comprises the reactant gases needed for photo bio growth in this gas.Like this, in jet process, the reactant gases needed for photo bio growth can not only be provided, and can fully stir photo bio nutrient solution, make greatly improving by optical efficiency of photo bio.Note that the gas assembled in bioreactor, can by the open discharge on its top.
When above-mentioned bioreactor is made up of two panels fexible film, the mode that the bottom of two panels fexible film and both sides of the edge are clamped by clips or by the mode of two panels fexible film bottom and both sides of the edge heat seal is sealed.Or the fexible film of above-mentioned formation bioreactor is a slice and sealing forms tubular film or above-mentioned fexible film is integral type tubular film, the mode that the lower edge of described tubular film is clamped by clips or sealed by the mode of heat seal.Wherein a slice fexible film formation tubular can adopt curling and the edge heat seal of curling rear coincidence is sealed or sealed by clips, and above-mentioned integral type tubular film refers to that the film selected is that tubular film does not need curling sealing.
Multiple isolating points in above-mentioned bioreactor, specifically can be formed by the mode arranging column or heat seal.Specifically comprise: column is oppositely arranged in the corresponding position of bioreactor both sides, the fexible film for clamping bioreactor both sides forms multiple isolating points; Generally be less than in the spacing of two columns of the corresponding position of bioreactor both sides the thickness being full of the liquid regions of liquid between the fexible film of bioreactor both sides.Or above-mentioned multiple isolating points is by being formed the mode of the select location heat seal on the fexible film of inner for bioreactor both sides.Note that the present invention is not limited to this, other method of clamping also can be adopted to form multiple isolating points, such as, elongated clip or other be applicable to any one fixture of the present invention.
Be illustrated in figure 2 by multiple isolating points realize point-like isolation effect schematic diagram, in Fig. 2 10 be fexible film, 20 for aerating apparatus, 30 be multiple isolating points.
Multiple bioreactor can also be arranged to a photo bio culture systems.
Be described in detail below by specific embodiment.
Embodiment one:
The bioreactor that the embodiment of the present invention one provides, its structure as shown in Figure 3, comprising: fexible film 11, multiple isolating points 12, aerating apparatus 13, liquid discharge entrance 14 and the suspension element be made up of hanger bar 15 and suspension line 16.
Above-mentioned fexible film 11 can be any transparent or semitransparent films such as polyethylene PE film, polyvinylchloride film, polyurethane PU film; And in the film of bioreactor both sides at least side be transparent or semitransparent material; The sealing the margin of above-mentioned fexible film 11 is sealed by the mode of heat seal or is sealed by clips.
Namely by the bottom of two panels fexible film 11 and both sides of the edge heat seal sealing or its underpart and both sides of the edge clips can be clamped, form the bag-like container with fluid continuity.Also tubular film can be sealed into a slice fexible film, or the tubular film of the integral type that need not seal directly selecting tubular flexible materials to make, and tubular film has at least half surface-area to be transparent or semitransparent material, the lower edge that now only need seal tubular film can form the bag-like container with fluid continuity.
Multiple isolating points 12 is set between fexible film 11, for after holding photo bio nutrient solution, photo bio nutrient solution is divided into some liquid regions be interconnected.Multiple isolating points 12 can be formed by the mode of heat seal, realizes bioreactor being divided into some regions with fluid continuity, can form the liquid regions be interconnected after holding nutrient solution.
Aerating apparatus 13 is arranged on the bottom of the liquid regions in bioreactor, and for providing the reactant gases needed for photo bio growth, enable the bottom-up aeration of gas, aeration effect is better.Such as: aerating apparatus 13 can be one and have some micropore breather pipes, as shown in Figure 3, ventpipe is incorporated into bottom from the top of bioreactor, then bending extends along bottom, and the ventpipe bottom being positioned at has some micropores, guarantee aeration bubbles is even, and the pressurized air of suitable pressure power passes into from ventpipe two ends.
Bioreactor top is provided with the sprung parts for hanging bioreactor, the sprung parts for being made up of hanger bar 15 and suspension line 16 in Fig. 3.Hanger bar 15 can also be only had in actual applications.Hanger bar 15 can be the straight-bar that steel pipe that intensity is suitable or other any material diameter are suitable, and pass from the top of reactor, there is suspension line 16 at the two ends of hanger bar 15, can be suspended on suitable place.
Bioreactor bottom is provided with the liquid discharge entrance 14 for injecting and get rid of photo bio nutrient solution.Nutrient solution and algae kind can add from upper opening, also can inject from lower drain port.
Such as: the bioreactor in Fig. 3 can be wide 1000 millimeters, the reactor of similar bag minor of high 1200 millimeters.The diameter of each isolating points utilizing heat seal to be formed can be 5 millimeter.Bottom clamp, arranges aerating apparatus in lower end base 50 millimeters of regions.Aerating apparatus can directly select micropore air aeration tube, also can adopt plastics tubing, plexi-glass tubular punch above (aperture 0.2-0.4 millimeter) make.Certainly these parameters just illustrate, can set as required in practical application.
Embodiment two:
The bioreactor that the embodiment of the present invention two provides, its structure as shown in Figure 4, comprising: fexible film 21, multiple isolating points 22, aerating apparatus 23, liquid discharge entrance 24 and the suspension element be made up of hanger bar 25 and suspension line 26.
The difference of the bioreactor provided in itself and embodiment one is that its aerating apparatus 23 is a straight ventpipe, and its gangway is all positioned at bottom.And aerating apparatus 23 is U pipe ventpipe in embodiment one, its gangway is all positioned at the top of bioreactor.
Multiple isolating points 22 is set between fexible film 21.With can by least one pair of column 101 of arranging, 102(see Fig. 8 unlike multiple isolating points 22 in embodiment one, the position in the front and back both sides corresponding with multiple isolating points 22) formed.Particularly, as shown in Figure 8, every column 101,102 is arranged in the both sides, front and back of bioreactor and front and back are relative, for the sandwich fexible film from bioreactor, each column 101,102 has the projection 12 ' for the formation of multiple isolating points 22.When every column 101,102 clamps the fexible film of bioreactor both sides, protruding 12 ' will form isolating points 22 on fexible film.
The fore-and-aft clearance being generally positioned at the projection 12 ' of every column 101,102 of the corresponding position of bioreactor both sides is less than the thickness being full of the liquid regions of liquid between the fexible film of bioreactor both sides, preferably, the fore-and-aft clearance of the projection 12 ' of every column of the corresponding position of bioreactor both sides is 0 ~ 1mm.
The width of liquid regions can be adjusted by the position adjusting column.Such as, the level interval being positioned at the column of fexible film homonymy is 55mm, and after ensureing loading nutrient solution, the horizontal clearance of liquid regions is not more than 45mm.
All the other structures of bioreactor are identical with embodiment one, repeat no more herein.
Embodiment three:
The bioreactor that the embodiment of the present invention three provides, its structure as shown in Figure 5, comprising: fexible film 31, multiple isolating points 32, aerating apparatus 33, liquid discharge entrance 34 and the suspension element be made up of suppending hole 35.
The difference of the bioreactor provided in itself and embodiment one is that its suspension element is suppending hole 35.By the suspension of the direct realization response device of suppending hole 35.Fexible film 31 top is pointed shape as shown in FIG., is provided with a suppending hole at sharp corner.
All the other structures of bioreactor are identical with embodiment one, repeat no more herein.
Embodiment four:
The bioreactor that the embodiment of the present invention four provides, its structure as shown in Figure 6, comprising: fexible film 41, multiple isolating points 42, aerating apparatus 43, liquid discharge entrance 44 and the suspension element be made up of suppending hole 45.
Although its aerating apparatus 43 is also U pipe ventpipe, its gangway is all positioned at the top of bioreactor, is that all direct upper square tube by reactor of the discrepancy end of its U-shaped ventpipe gets off with the difference of the bioreactor provided in embodiment three.
The difference of the bioreactor provided in itself and embodiment one is also that its suspension element is suppending hole 45.By the suspension of the direct realization response device of suppending hole 45.Fexible film 41 top is provided with row's suppending hole 45 as shown in FIG., by the steady suspension of this row's suppending hole 45 realization response device.
All the other structures of bioreactor are identical with embodiment one, repeat no more herein.
Embodiment five:
The embodiment of the present invention five provides a kind of photo bio cultural method, and the bioreactor using above-described embodiment one to four to provide cultivates photo bio.The method is mainly for the bioreactor adopting clips to seal fexible film.The method comprises:
Photo bio nutrient solution is contained in and is cultivated by having in the bioreactor of fluid continuity bag-like container of being formed of fexible film.
When needing to gather in the crops the photo bio of having cultivated, the lower edge of two panels fexible film and/or the clips of both sides of the edge that form bioreactor are opened, the lower edge of two panels fexible film and/or both sides of the edge are opened, photo bio nutrient solution is discharged and collects; The clips of the lower edge of the tubular film maybe a slice fexible film sealing of the integral type tubular film or formation bioreactor that form bioreactor formed is opened, the lower edge of tubular fexible film is opened, photo bio nutrient solution is discharged and collects.
That is, when the photosynthetic microorganism in above-mentioned bioreactor has been cultivated, for the bioreactor that the bottom and both sides of the edge that are clamped two panels fexible film by clips are formed, the bottom of two panels fexible film and/or the clips of both sides of the edge can be opened, directly the photo bio nutrient solution in bioreactor is collected in collecting tank or other collection devices.When the bottom of both sides fexible film and both sides of the edge are clips sealing, only can open the clips at the edge of bottom or both sides, also clips all can be opened and gather in the crops.When two panels fexible film is integral type tubular film, the clips can only opening lower edge is gathered in the crops.
When photo bio has been cultivated, the bioreactor of above-mentioned employing clips sealing can carry out a point demolition clean.Namely, when the bottom of two panels fexible film and/or the clips of both sides of the edge are opened, the part in the middle of described two panels fexible film is cleaned; Or when the clips of the lower edge of the tubular film that integral type tubular film or the sealing of a slice fexible film are formed is opened, the part in the middle of cleaning tubular fexible film.That is, when cleaning, the bottom that also two panels fexible film can be clamped by clips and the clips of both sides of the edge are opened, and directly clean the middle portion of two panels fexible film.
This harvesting approach is directly gathered in the crops under gravity, realizes conveniently and energy efficient.This cleaning way, not only easy to clean and can not there is cleaning dead angle, avoid cleaning difficulty and the sordid phenomenon of cleaning occurs.
The various bioreactors provided in above-described embodiment one to four, the photo bio culture systems comprising two or more bioreactors can be combined into, for the bioreactor shown in embodiment one, the structure of the photo bio culture systems formed as shown in Figure 7, the transport pipe 2 comprising some bioreactors 10 and be connected with the liquid discharge entrance of bioreactor.
Transport pipe, for injecting the photo bio nutrient solution of photo bio nutrient solution and/or collection bioreactor in bioreactor.
Bioreactor is hung by sprung parts and places, and for holding photo bio nutrient solution, cultivates photosynthetic microorganism.
Coupled together by some bioreactors above by pipeline, can unify to carry out filling nutrient solution, inoculation, technological operation such as emptying grade, realizes mass production and the management of photo bio.
The method flow of the various bioreactors provided in above-described embodiment is provided, performs step as follows:
Step S101: at least will seal by a slice fexible film, and form the bag-like container with fluid continuity.
Above-mentioned at least a slice fexible film of inciting somebody to action seal, and specifically comprise:
The mode clamped by clips or by by the mode of two panels fexible film edge heat seal, by the bottom of two panels fexible film and both sides of the edge sealing.Or seal the tubular film formed or the integral type tubular film selected by by a slice fexible film, the mode lower edge of tubular film clamped by clips or sealed by the mode of heat seal.
Step S102: multiple isolating points is set between the fexible film of the inner both sides of bioreactor, photo bio nutrient solution is divided into some liquid regions be interconnected.
Multiple isolating points is set between the fexible film of the inner both sides of bioreactor, specifically comprises:
At the column that the corresponding position setting position of bioreactor both sides external is relative, formed multiple isolating points of bioreactor inside by the fexible film of column clamping bioreactor both sides; Or pass through, by the mode of the fexible film heat seal of inner for bioreactor both sides, to form multiple isolating points at the select location place of fexible film.
The thickness of liquid regions between the fexible film being less than bioreactor both sides in the spacing of two columns of the corresponding position of bioreactor both sides.
Step S103: the sprung parts for hanging bioreactor is set on bioreactor top.
Sprung parts can be the sprung parts being arranged on the suppending hole on fexible film top, hanger bar or being made up of hanger bar and suspension line.Specifically see the specific descriptions of the above embodiments.
Step S104: the liquid discharge entrance for injecting and get rid of photo bio nutrient solution is set in two panels fexible film bottom.
Step S105: the bottom being positioned at liquid regions in bioreactor arranges aerating apparatus, provides the reactant gases needed for photo bio growth.
For the film type reactor using clips clamping, when photosynthetic microorganism (such as: micro-algae) cultivates ripe results, according to the mode making micro-algae sink to collecting, then sealed bottom folder can be opened and release the micro-algae being deposited in bottom and gather in the crops; According to the mode making micro-algae floating collect, then micro-algae of directly taking out top is gathered in the crops, and greatly reduces results cost.
At the end of cultivation, by the removal such as clips and column, cleaning can be facilitated, avoids cleaning dead angle.Namely when needing the bioreactor sealed above-mentioned employing clips mode to gather in the crops and/or clean, the bottom of two panels fexible film and/or the clips of both sides of the edge are opened, the bottom of two panels fexible film and/or both sides are opened, results photo bio and/or the part of cleaning in the middle of two panels fexible film; Or the clips of the lower edge of integral type tubular film is opened, the bottom of tubular fexible film is opened, results photo bio and/or the part of cleaning in the middle of tubular fexible film.
In the preparation method of above-mentioned bioreactor, the order of step S101-105 can adjust, and does not limit and first prepares which parts, as long as can prepare reactor.
Above-mentioned bioreactor that the embodiment of the present invention provides and preparation method thereof, photo bio culture systems and method, liquid regions is formed by isolating points, nutrient solution no longer goes up lower leaf, avoids the problem of the aeration poor effect that lower leaf causes in the transverse movement of existing film type bioreactor culture liquid; Bioreactor of the present invention, at the bottom aeration of liquid regions, can obtain good aeration effect, and analyzed by concrete fluid software simulation, the stirring effect of above-mentioned bioreactor is better than the stirring effect of existing reactor.Relative to pipeline reactor, plate-type reactor and existing film reactor, its CO2 utilization ratio is improved, and in reactor, the dissolved oxygen amount of nutrient solution improves, and is more conducive to the growth of photo bio.And when avoiding lateral isolation in picture prior art with isolating points isolation, the photo bio in photo bio nutrient solution deposits to the possibility on lateral isolation band, thus be easy to cleaning and results.
And above-mentioned bioreactor adopts the training method of not circulating liquid, only bioreactor need be hung and place, there is not the shortcoming that nutrient solution transporting resistance is large, owing to not needing Cyclic culture liquid, saved energy consumption, reduced toxigenic capacity; It belongs to open system, also there is not the too high shortcoming affecting micro algae growth of dissolved oxygen.
When after microdisk electrode results, the clips etc. of the column of setting, use can be disassembled, clean reactor, directly clean sheet fexible film, convenient and swift, cleaning performance is good.And due to easy disassembly, reduce transport installation cost.
Film type bioreactor provided by the invention, by appropriate design, makes this reactor: (1) has high illuminating surface and amasss the ratio with volume; (2) there is higher gas exchange efficiency; (3) there is applicable Recycle design; (4) route of transmission of light, distribution and quality is improved; (5) accumulation of unwanted metabolic products is prevented.
Multiple reactor can be combined by pipeline, that one is suitable for batch industrialization management bioreactor, and avoid general plastic bag type reactor optical length by the mode forming liquid regions, be unfavorable for the shortcoming of high-density culture, enable to be obtained better lighting effect by the photo bio of cultivating, better complete photosynthetic response.
In the present invention, multiple isolating points can be arranged in the pattern of rule, and such as a line a row or column one arranges ground arrangement, manufacture more convenient like this, but the multiple isolating points in the present invention also can be arranged in any irregular pattern.
Obviously, those skilled in the art can carry out various change and modification to the present invention and not depart from the spirit and scope of the present invention.Like this, if these amendments of the present invention and modification belong within the scope of the claims in the present invention and equivalent technologies thereof, then the present invention is also intended to comprise these change and modification.
Claims (9)
1. the pocket type bioreactor be made up of fexible film, described bioreactor is formed with the liquid discharge entrance (14) for injecting and get rid of photo bio nutrient solution, and in described bioreactor, be provided with aerating apparatus (13)
It is characterized in that:
Multiple isolating points (12) is formed in described bioreactor, for the photo bio nutrient solution in bioreactor is divided into multiple liquid regions be interconnected,
Described aerating apparatus (13) is from the bottom of described bioreactor to jet flow stream in photo bio nutrient solution, and described gas comprises the reactant gases needed for photo bio growth;
Mutually be clamped together by the front side fexible film and rear side fexible film making bioreactor and form described multiple isolating points (12);
Also comprise the both sides, front and back that are arranged in described bioreactor and relative at least one pair of column (101,102) in front and back, for the sandwich fexible film from described bioreactor, each column (101,102) has the projection (12 ') for the formation of described multiple isolating points (12).
2. bioreactor as claimed in claim 1, it is characterized in that, the fore-and-aft clearance between the projection (12 ') of described every column (101,102) is 0 ~ 1mm.
3. bioreactor as claimed in claim 1, is characterized in that,
Described bioreactor is made up of two panels fexible film, and the mode that the lower edge of described two panels fexible film and both sides of the edge are clamped by clips seals,
When adopting the mode of clips clamping, the clips of the lower edge that described two panels fexible film is clamped by clips and both sides of the edge is opened when gathering in the crops or clean.
4. bioreactor as claimed in claim 1, it is characterized in that, described bioreactor is made up of a slice fexible film, and described a slice fexible film is folded into two-layer, and the mode that the open sides edge formed after folding and/or lower edge are clamped by clips seals
When adopting the mode of clips clamping, the clips of the lateral edges that described a slice fexible film is clamped by clips and/or lower edge is opened when gathering in the crops or clean.
5. bioreactor as claimed in claim 1, it is characterized in that, described bioreactor is made up of integral type tubular fexible film, and the mode that the lower edge of described tubular fexible film is clamped by clips seals,
When adopting the mode of clips clamping, the clips of the lower edge that described tubular fexible film is clamped by clips is opened when gathering in the crops or clean.
6. bioreactor according to claim 1, is characterized in that, the top of described bioreactor is provided with the sprung parts (15,16) hung.
7. bioreactor as claimed in claim 1, it is characterized in that, described sprung parts comprises: the suspension element that suppending hole, hanger bar or hanger bar and suspension line form.
8. the bioreactor according to any one of claim 1-7, is characterized in that, described fexible film selects the film of llowing group of materials: polyethylene PE film, polyvinylchloride film or polyurethane PU film.
9. a photo bio culture systems, is characterized in that, comprising:
At least one bioreactor according to any one of claim 1-8; With
The transport pipe be connected with the liquid discharge entrance of described bioreactor, for injecting photo bio nutrient solution and/or collecting the photo bio nutrient solution of described bioreactor in described bioreactor.
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| CN104862207B (en) * | 2014-02-25 | 2017-10-10 | 中国科学院青岛生物能源与过程研究所 | A kind of hanging bag type microalgae Photoreactor and hanging bag type both culturing microalgae system |
| CN106434278B (en) * | 2016-08-26 | 2019-10-18 | 浙江天地环保科技有限公司 | A kind of two sections of multi-stage aeration large size gas lift flat plate photobioreactors |
| TWI678416B (en) * | 2017-03-07 | 2019-12-01 | 國立臺灣海洋大學 | Closed-type liquid culture system and method thereof for microorganisms |
| JP2021527562A (en) * | 2018-06-21 | 2021-10-14 | バテル・メモリアル・インスティテュートＢａｔｔｅｌｌｅ Ｍｅｍｏｒｉａｌ Ｉｎｓｔｉｔｕｔｅ | Improved microchannel device or mesochannel device, and its additive manufacturing method |
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