CN106434278B - A kind of two sections of multi-stage aeration large size gas lift flat plate photobioreactors - Google Patents

A kind of two sections of multi-stage aeration large size gas lift flat plate photobioreactors Download PDF

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CN106434278B
CN106434278B CN201610742506.9A CN201610742506A CN106434278B CN 106434278 B CN106434278 B CN 106434278B CN 201610742506 A CN201610742506 A CN 201610742506A CN 106434278 B CN106434278 B CN 106434278B
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bioreactor
aeration
reactor shell
main line
internal partition
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CN106434278A (en
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李清毅
水双
赵金龙
厉雄峰
徐轶伦
胡达清
李立
王汝能
姚子麟
刘海蛟
张健
邱鹤
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Zhejiang Zheneng Technology Environmental Protection Group Co ltd
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Zhejiang Tiandi Environmental Protection Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/58Reaction vessels connected in series or in parallel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a kind of two sections of multi-stage aeration large size gas lift flat plate photobioreactors, including bioreactor, the bioreactor include reactor shell, internal partition, aerating system and exhaust system;The internal partition is set to the centre of bioreactor, bioreactor is separated into two regions, there are certain distances for internal partition and reactor shell top and bottom, circulate convenient for algae solution;The aerating system includes motor-driven valve, restricting orifice, aeration main line and multiple aeration branch pipes.The beneficial effects of the present invention are: the scale application of microalgae carbon-fixation-technology compared with laboratory research, may be implemented in the present invention;This invention ensures that CO2Sufficient supply in bioreactor, and solve CO2The problem of gas is unevenly distributed;The invention can ensure that algae solution is not influenced by extraneous factor, and solve the problems, such as that bioreactor dissolved oxygen is difficult to be discharged.

Description

A kind of two sections of multi-stage aeration large size gas lift flat plate photobioreactors
Technical field
The technology belongs to the fixed coal-burning power plant CO of microalgae2Field, in particular to it is a kind of for the fixed CO of microalgae2Large size it is flat Board-like bioreactor.
Background technique
By CO2" greenhouse effects " caused by discharge and global warming have become great environmental problem, the CO that coal electricity releases2 Occupy larger specific gravity.To solve the CO that coal-burning power plant releases2Emission problem, microalgae carbon-fixation-technology be great prospect technology it One.Microalgae has many requirements, temperature, illumination and photoperiod, CO to breeding environment2Concentration etc. all has an impact to it, therefore selects Suitable bioreactor is most important.
Bioreactor currently used for microalgae carbon sequestration mainly has open and two kinds closed.Open culture with it is outer Boundary's environment directly contacts, and microalgae realizes photoautotrophic cultivation using natural light, but it has the following problems: taking up a large area, cultivates Conditional instability, vulnerable to pollution are affected by the external environment big.The density of closed photo bioreactor culture microalgae is high, culture Condition is easy to control, the microalgae safety non-pollution turned out.The diversified closed reactor of development in laboratory, but problem It is to be only limitted to carry out small research mostly, there are CO for the application of scale large size2Gas is unevenly distributed, and dissolved oxygen is difficult to arrange in algae solution Out, microalgae growth rate affected by many factors is different, is not suitable for the problems such as sea-farming.
Summary of the invention
The present invention for present reactor be difficult to the application of scale large size, in algae solution dissolved oxygen be difficult to be discharged, algae growth speed The problem of rate is different and is not suitable for sea-farming, invents and devises a kind of multi-stage aeration large-scale flat-plate type bioreactor, The large-scale application of microalgae carbon-fixation-technology is aimed at, improves the carbon sequestration efficiency of large-scale bioreactor to greatest extent.
In order to achieve the above technical purposes, the invention adopts the following technical scheme:
This two sections of multi-stage aerations large size gas lift flat plate photobioreactor, including bioreactor, the photoproduction Object reactor includes reactor shell, internal partition, aerating system and exhaust system;The internal partition is set to photo-biological Bioreactor is separated into two regions, stayed at the top and bottom of internal partition and reactor shell by the centre of reactor There is certain distance, is circulated convenient for algae solution;The aerating system includes motor-driven valve, restricting orifice, aeration main line and more A aeration branch pipe;Reactor shell two sides are provided with the 5th aeration main line and the 6th aeration main line, respectively from reactor Housing bottom and middle part pass through multiple aeration branch pipes access reactor shell;The exhaust system includes shut-off valve and connecting tube Road, exhaust pipe are bend pipe, are arranged at the top of reactor shell, the gas in algae solution can be made to be discharged, while guaranteeing optical-biological reaction Algae solution is not contaminated inside device.
As preferred: the reactor shell is box rectangular parallelepiped structure, rectangular body side surface setting working medium import and work Matter outlet, side and the multiple aeration openings of setting etc., exhaust outlet is arranged in top.
As preferred: cuboid bottom uses corrosion resisting steel, remaining five face bilayer white tempering glass strong using light transmission Add PVB film among glass.
As preferred: the length of cuboid is 4m-5m, width 400mm, is highly 4m-5m;The corrosion resistant of cuboid bottom It loses steel and uses 907 stainless steels;The white tempered glass of single layer in remaining five face of cuboid is with a thickness of 12mm.
As preferred: internal partition is 0.2m-0.3m with reactor shell distance from bottom, with reactor shell distance from top For 0.8m-1m.
As preferred: the 5th aeration main line aeration flow is 2 times that the 6th aeration main line is aerated flow.
As preferred: the bend pipe quantity at the top of reactor shell is three.
As preferred: the 5th aeration main line and the 6th aeration main line flow are different, are guaranteeing that two sides algae solution can be into While the internal partial circulating of row flows, the two sides of internal partition can carry out whole circulate.
As preferred: the bioreactor includes multistage, and series is not more than 10.
As preferred: passing through the respectively arranged pipe joint chair in bioreactor bottom and middle part and flange phase between every grade Even.
The beneficial effects of the present invention are:
1, the scale application of microalgae carbon-fixation-technology may be implemented compared with laboratory research in the present invention.
2, this invention ensures that CO2Sufficient supply in bioreactor, and solve CO2What gas was unevenly distributed Problem.
3, it the invention can ensure that algae solution is not influenced by extraneous factor, and solves bioreactor dissolved oxygen and is difficult to be discharged The problem of.
4, the present invention realizes algae solution and realizes that partial circulating flowing and entirety circulate in bioreactor, improves The carbon sequestration efficiency of microalgae ensure that the growth rate of microalgae is more uniform.
5, the present invention rationally designs bioreactor structure and selection bioreactor shell material, ensure that reaction Device sea-farming preferably.
Detailed description of the invention
Fig. 1 is the schematic front view of two sections of multi-stage aeration large-scale flat-plate type bioreactors of the invention;
Fig. 2 is the sorrowful view schematic diagram of two sections of multi-stage aeration large-scale flat-plate type bioreactors of the invention;
Fig. 3 is two sections of multi-stage aeration large-scale flat-plate type bioreactor schematic diagram of housing structure of the invention.
Description of symbols: 2, (n-1)th grades of first order bioreactor 1, second level bioreactor photo-biologicals are anti- Answer 4, n-th grades of bioreactors 5 of device, the first bottom pipe joint chair 6, the second bottom pipe joint chair 7, the first middle part pipe joint chair 8, second Middle part pipe joint chair 9, third bottom pipe joint chair 10, the 4th bottom pipe joint chair 11, third middle part pipe joint chair 12, the 4th middle part pipe joint chair 13, the 5th bottom pipe joint chair 14, the 6th bottom pipe joint chair 15, the 5th middle part pipe joint chair 16, the 6th middle part pipe joint chair 17, reactor Shell 18, internal partition 19, aerating system 20, exhaust system 21, cuboid bottom 22, left side 23, right side 24, cuboid 26, working medium import 27, sender property outlet 28, bend pipe 29, restricting orifice 30, motor-driven valve 31, first are aerated at the top of side 25, cuboid Main line 32, second is aerated main line 33, aeration branch pipe 34, third aeration main line the 35, the 4th and is aerated main line 36, shut-off valve 37, the 5th aeration main line the 38, the 6th is aerated main line 39.
Specific embodiment
The present invention is described further below with reference to embodiment.The explanation of following embodiments is merely used to help understand this Invention.It should be pointed out that for those skilled in the art, without departing from the principle of the present invention, also Can be with several improvements and modifications are made to the present invention, these improvement and modification also fall into the protection scope of the claims in the present invention It is interior.
As shown in Figure 1 to Figure 3, this two sections of multi-stage aerations large size gas lift flat plate photobioreactor includes first order light Bioreactor 1,2, (n-1)th grades of second level bioreactor, 4, n-th grades of bioreactor bioreactor 5;
A side bottom and middle part for the first order bioreactor 1 and n-th grade of bioreactor 5 is respectively set Pipe joint chair, the second level bioreactor 2 and (n-1)th grade of 4 two sides of bioreactor are arranged in bottom and middle part Pipe joint chair.
The lower tube of first order bioreactor 1 the bottom pipe joint chair 6 and 2 side of second level bioreactor Joint chair 7 is connected by flange, in 1 middle part pipe joint chair 8 of first order bioreactor and 2 side of second level bioreactor Portion's pipe joint chair 9 is connected by flange;
The bottom pipe joint chair 11 and the n-th -2 grades bioreactors 3 one of (n-1)th grade of 4 side of bioreactor The bottom pipe joint chair 10 of side is connected by flange, (n-1)th grade of 4 middle part pipe joint chair 13 of bioreactor and the n-th -2 grades photo-biologicals The middle part pipe joint chair 12 of 3 side of reactor is connected by the flange that is connected;
The bottom pipe joint chair 15 of n-th grade of bioreactor 5 and (n-1)th grade of 4 other side of bioreactor Bottom pipe joint chair 14 is connected by flange, n-th grade of 5 middle part pipe joint chair 17 of bioreactor and (n-1)th grade of bioreactor 4 The middle part pipe joint chair 16 of the other side is connected by flange.N is not more than 10.
The bioreactor includes reactor shell 18, internal partition 19, aerating system 20, exhaust system 21.
The reactor shell 18 is box rectangular parallelepiped structure, and cuboid bottom 22 uses corrosion resisting steel, remaining five face Using the white tempered glass centre of the strong bilayer of light transmission plus PVB film.Working medium import 27 and working medium is arranged in rectangular body side surface 25 Multiple aeration openings etc. are arranged in outlet 28, side 23 and 24, and exhaust outlet is arranged in top 26.The length of cuboid is 4m-5m, and width is 400mm is highly 4m-5m.The corrosion resisting steel of cuboid bottom 22 uses 907 stainless steels.The single layer in remaining five face of cuboid is white Tempered glass is with a thickness of 12mm.
The internal partition 19 is set to the centre of bioreactor, and bioreactor is separated into the area Liang Ge There are certain distances for domain, internal partition 19 and reactor shell top 26 and bottom 22, circulate convenient for algae solution.Inside every Plate 19 and reactor shell bottom 22 are 0.8m-1m with 26 distances at the top of reactor shell apart from being 0.2m-0.3m.
The aerating system 20 includes motor-driven valve 31, restricting orifice 30, aeration main line 32,33,35,36 and multiple exposures Gas branch pipe 34 etc.;18 two sides of reactor shell are respectively provided with two-way aeration main line 38,39, respectively from reactor shell bottom in Portion accesses reactor shell 18 by multiple aeration branch pipes.It is aerated the flow of main line 38 and 39 difference, is guaranteeing two sides algae While liquid can carry out the flowing of internal partial circulating, the two sides of internal partition 19 can carry out whole circulate.It is aerated main line 38 aeration flows are 2 times for being aerated main line 39 and being aerated flow.
The exhaust system 21 includes shut-off valve 37 and connecting line, and exhaust pipe is bend pipe 29, is arranged in reactor enclosure 26 at the top of body, the gas in algae solution can be made to be discharged, while guarantee that algae solution is not contaminated inside bioreactor.Reactor shell 29 quantity of bend pipe at top 26 is 3.

Claims (3)

1. a kind of two sections of multi-stage aeration large size gas lift flat plate photobioreactors, it is characterised in that: including bioreactor, The bioreactor includes multistage, and series is not more than 10, is distinguished between every grade by bioreactor bottom and middle part The pipe joint chair of setting is connected with flange;The bioreactor includes reactor shell (18), internal partition (19), aeration system System (20) and exhaust system (21);The internal partition (19) is set to the centre of bioreactor, by optical-biological reaction Device is separated into two regions, and there are certain distances for internal partition (19) and reactor shell top (26) and bottom (22), just It is circulated in algae solution;The aerating system (20) includes motor-driven valve (31), restricting orifice (30), aeration main line and multiple Aeration branch pipe;Reactor shell (18) two sides are provided with the 5th aeration main line (38) and the 6th aeration main line (39), point Reactor shell (18) are not accessed from reactor shell bottom and middle part by multiple aeration branch pipes;5th aeration main line (38) It is different with the 6th aeration main line (39) flow, it is internal while guaranteeing that two sides algae solution can carry out the flowing of internal partial circulating The two sides of partition (19) can carry out whole circulate;The exhaust system (21) includes shut-off valve (37) and connecting line, Exhaust pipe is bend pipe (29), is arranged at the top of reactor shell (26), the gas in algae solution can be made to be discharged, while guaranteeing photo-biological Inside reactor algae solution is not contaminated;Reactor shell (18) is box rectangular parallelepiped structure, and working medium is arranged in rectangular body side surface (25) Multiple aeration openings, top (26) setting exhaust is arranged in import (27) and sender property outlet (28), left side (23) and right side (24) Mouthful;Cuboid bottom (22) uses corrosion resisting steel, remaining five face is using the white tempered glass centre of the strong bilayer of light transmission plus PVB Film;The length of cuboid is 4m-5m, width 400mm, is highly 4m-5m;The corrosion resisting steel of cuboid bottom (22) uses 907 stainless steels;The white tempered glass of single layer in remaining five face of cuboid is with a thickness of 12mm;Internal partition (19) and reactor shell bottom Portion (22) distance is 0.2m-0.3m, is 0.8m-1m with (26) distance at the top of reactor shell.
2. two sections of multi-stage aerations large size gas lift flat plate photobioreactor according to claim 1, it is characterised in that: 5th aeration main line (38) aeration flow is 2 times that the 6th aeration main line (39) is aerated flow.
3. two sections of multi-stage aerations large size gas lift flat plate photobioreactor according to claim 1, it is characterised in that: Bend pipe (29) quantity of (26) is three at the top of reactor shell.
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