CN107446796A - A kind of bacteria cellulose fermenting and producing equipment - Google Patents
A kind of bacteria cellulose fermenting and producing equipment Download PDFInfo
- Publication number
- CN107446796A CN107446796A CN201610367656.6A CN201610367656A CN107446796A CN 107446796 A CN107446796 A CN 107446796A CN 201610367656 A CN201610367656 A CN 201610367656A CN 107446796 A CN107446796 A CN 107446796A
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- China
- Prior art keywords
- culture box
- culture
- bacteria cellulose
- height
- baffle plate
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- 229920002678 cellulose Polymers 0.000 title claims abstract description 56
- 239000001913 cellulose Substances 0.000 title claims abstract description 56
- 241000894006 Bacteria Species 0.000 title claims abstract description 54
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 230000004151 fermentation Effects 0.000 claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims description 23
- 238000009630 liquid culture Methods 0.000 claims description 17
- 239000007788 liquid Substances 0.000 claims description 11
- 230000003068 static effect Effects 0.000 claims description 8
- 239000002699 waste material Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 235000015097 nutrients Nutrition 0.000 description 7
- 239000002609 medium Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 3
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 229920002749 Bacterial cellulose Polymers 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000005016 bacterial cellulose Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 210000004087 cornea Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000033 toxigenic Toxicity 0.000 description 1
- 230000001551 toxigenic effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention provides a kind of bacteria cellulose fermenting and producing equipment, including body and culture box, for quiescent culture bacteria cellulose.The bacteria cellulose fermenting and producing occupation area of equipment is small, multiple culture box fermenting and producings can be carried out simultaneously, the bacteria cellulose film of different-thickness or required thickness can be produced simultaneously, and just all culture mediums can be added in multiple culture boxes by once-through operation, save manual labor, the fermentation yield and yield of bacteria cellulose are greatly improved, finally improves the efficiency of bacteria cellulose production.
Description
Technical field
The invention belongs to microbial cultivation equipment field, it particularly relates to a kind of fermenting and producing equipment for quiescent culture bacteria cellulose.
Background technology
Bacteria cellulose(Bacterial cellulose, abbreviation BC), refer to the general designation of cellulose under given conditions, generated by the microbial fermentation of some special Pseudomonas.The fermentation synthetic method of bacteria cellulose has two kinds of static fermentation and dynamic fermentation, wherein static fermentation refers to that bacterium carries out fermented and cultured in the liquid culture medium of standing and the gas-liquid contact face of air and produces cellulose, bacteria cellulose is from nutrient solution and the gas-liquid interface of air, along nutrient solution continuous dynamic accumulation downwards, ultimately forming thickness and nutrient solution highly has positively related bacteria cellulose film.It is domestic at present to use static fermentation method using Microbe synthesis bacteria cellulose is most.
The membranaceous bacteria cellulose compact structure that static fermentation method is formed, the anti-intensity height that rises, air-and water-permeable performance is good, retentiveness is good, there is good biocompatibility and biodegradability simultaneously, it is widely used in each field such as medical science, biology, chemical industry, food, such as Bone Defect Repari, cornea, wound dressing, tissue engineering bracket.But the space and manual labor needed for during static culture are larger, toxigenic capacity is high, yield and low yield, and these all become the principal element for restricting bacteria cellulose industrialized production and popularization and application.In addition, in the actual production process of bacteria cellulose simultaneously, it is often necessary to which one time fermentation just produces the bacteria cellulose film of different-thickness, and to meet industrial requirement, but current static fermentation equipment all fails to realize this effect.
The content of the invention
The present invention provides a kind of equipment of the fermented-producing bacteria cellulose under static conditions, the occupation area of equipment is small, multiple culture box fermenting and producings can be carried out simultaneously, the bacteria cellulose film of different-thickness or required thickness can be produced simultaneously, and just all culture mediums can be added in multiple culture boxes by once-through operation, manual labor is saved, greatly improves the fermentation yield and yield of bacteria cellulose, finally improves the efficiency of bacteria cellulose production.
The present invention provides a kind of bacteria cellulose fermenting and producing equipment, including body and culture box, wherein,
The body includes roof 1, bottom wall, antetheca, rear wall, left wall, right wall;
The inner side of wherein described antetheca and rear wall is provided with multiple slide rails being parallel to each other, and the slide rail on antetheca and rear wall correspond to it is contour;In the left wall and right wall, with upper and lower two slide rails at intervals of height, opening in opposite direction is arranged in sequence with from top to bottom;
The culture box has a bottom surface and four side boards, the height of four described side boards is different, wherein the height of highest baffle plate is equal with the interval height of two slide rails up and down on the body, and on the side dams of highest baffle plate, provided with the hole and sliding block for controlling liquid culture medium height;The height of the medium liquid in culture box can be controlled by position of the adjusting slider in hole, so as to produce the bacteria cellulose film of different-thickness;
The distance between highest baffle plate and its side dams of the culture box, less than the distance between left wall and right wall in body;The culture carriage can move left and right on the slide rail of the body along slide rail.
Feed pipe and gas outlet are additionally provided with the roof of the body;Waste liquid effuser and air inlet are additionally provided with the bottom wall of the body.
Temperature-adjusting device is also equipped with the antetheca or rear wall of the body, the thermostatic quantity installs one or more according to the big I of culture device, so that the temperature in culture device is uniform, controllable.
Preferably, the height of the highest baffle plate of the interval height of upper and lower two slide rails and the culture box is 5-15 centimetres.
Preferably, the height of other three baffle plates of the culture box can each independent 2-3 centimetres lower than the height of highest baffle plate.
Preferably, the height of other three baffle plates of the culture box can be independently each 3-13 centimetres.
Preferably, the quantity of the hole on the culture box baffle plate and sliding block is selected from one or more, such as 2-5.
Preferably, the pore shape commonly used in the art such as the pore shape selection rectangle on the culture box baffle plate, ellipse, arch.
Preferably, around the hole on the culture box baffle plate, it is additionally provided with the scale designation of display liquid culture medium height.
Preferably, on the outside of the hole on the culture box baffle plate, flow guiding structure is additionally provided with, to guide nutrient solution to flow into next layer of culture box.
Preferably, the quantity for the culture box that can be placed in the bacteria cellulose fermenting and producing equipment is 10-20.
The bacteria cellulose fermenting and producing equipment of the present invention can be used for carrying out the bacteria cellulose film that quiescent culture is large batch of, thickness is different, and can realize and just all culture mediums are added in multiple culture boxes by once-through operation.
When in use, first it will be used to control the sliding block of liquid culture medium height to be fixed to required position on each culture box, each culture box is set to load the liquid culture medium of different height according to needs are produced, the bacteria cellulose film of different-thickness is gone out so as to fermenting and producing.Then culture carriage is pushed into close, makes bacteria cellulose fermenting and producing equipment integral sealing on the slide rail of body.
Then, inside the first floor culture box that feed pipe at the top of liquid culture medium slave unit after advance inoculation is injected into equipment, when the culture medium liquid level in first floor culture box, which reaches, cultivates the liquid level set by box top shoe, fluid nutrient medium can be from the hole above sliding block(Or through the flow guiding structure on the outside of hole)It is flowed into next layer of culture box.By that analogy, with the continuous injection of liquid culture medium, culture medium can from first floor culture box step by step fill up all culture boxes, now the culture medium in each culture box highly is beforehand through the height of sliding block setting, then carries out fermented and cultured.
After fermented and cultured terminates, any one layer of culture box can be pulled out as needed from the left and right sides of bacteria cellulose fermenting and producing equipment, the collection of bacteria cellulose is carried out and culture box is cleaned.
The present invention is simple in construction, easy to use, floor space is small, multiple culture box fermentations can be carried out simultaneously, the bacteria cellulose film of different-thickness or required thickness can be produced simultaneously, and just all culture mediums can be added in multiple culture boxes by once-through operation, manual labor is saved, is greatly improved the fermentation yield and yield of bacteria cellulose.
Brief description of the drawings
Fig. 1:The structural representation of the bacteria cellulose fermenting and producing apparatus body of the present invention.
Fig. 2:The structural representation of the bacteria cellulose fermenting and producing equipment culture box of the present invention.
Fig. 3:Another structural representation of the bacteria cellulose fermenting and producing equipment culture box of the present invention.
Fig. 4:The front section view of the bacteria cellulose fermenting and producing equipment of the present invention.
Embodiment
The present invention is described in more detail for Structure Figure and embodiment below, artisan will appreciate that the change made without departing from the scope and spirit of the present invention, belongs to the scope of the present invention.
Embodiment 1:
The present embodiment is a kind of preferred structure of the bacteria cellulose fermenting and producing equipment of the present invention.As depicted in figs. 1 and 2, the bacteria cellulose fermenting and producing equipment of the present embodiment includes body and culture box, wherein,
The body includes roof 1, bottom wall, antetheca 2, rear wall, left wall, right wall 3;The inner side of wherein described antetheca and rear wall is provided with multiple slide rails 4 being parallel to each other, and the slide rail on antetheca and rear wall correspond to it is contour;In the left wall and right wall, with upper and lower two slide rails at intervals of height, opening 5 in opposite direction is arranged in sequence with from top to bottom;
Feed pipe 6 and gas outlet 7 are additionally provided with the roof of the body;
Waste liquid effuser 8 and air inlet 9 are additionally provided with the bottom wall of the body.
The culture box has a bottom surface and four side boards, the height of four side boards is different, wherein the height of highest baffle plate 10 is equal with the interval height of two slide rails 4 up and down on body, highly it is 10 centimetres, other three heights of baffle plate are 8 centimetres, on the side dams 11 of highest baffle plate 10, provided with a vertical hole 12, the sliding block 13 for being used for adjusting liquid culture medium height is provided with hole;The sliding block can control the height of the medium liquid in culture box, so as to produce the bacteria cellulose film of different-thickness, around the hole on the culture box baffle plate, be additionally provided with the scale designation 14 of display liquid culture medium height;
In the present embodiment, the distance between the highest baffle plate 10 of the culture box and its side dams 11 are 25 centimetres, and the distance between left wall and right wall are 28 centimetres in body;The culture carriage can move left and right on the slide rail 4 of the body along slide rail 4.
Embodiment 2:
The present embodiment is another preferred structure of the bacteria cellulose fermenting and producing equipment of the present invention.As shown in figures 1 and 3, the bacteria cellulose fermenting and producing equipment of the present embodiment includes body and culture box, wherein the body is identical with the structure of embodiment 1.
The culture box has a bottom surface and four side boards, the height of four side boards is different, wherein the height of highest baffle plate 10 is equal with the interval height of two slide rails 4 up and down on body, highly it is 12 centimetres, other three heights of baffle plate are 9 centimetres, on the side dams 11 of highest baffle plate 10, provided with two vertical holes 12, one of hole is located at baffle plate top, another hole is located at baffle plate bottom, and the sliding block 13 for adjusting liquid culture medium height is designed with each hole;By setting position of the two sliding blocks in two holes, more different height can be set, so as to produce the bacteria cellulose film of more different-thickness, around the hole on the culture box baffle plate, the scale designation 14 of display liquid culture medium height is additionally provided with.
In the present embodiment, the distance between the highest baffle plate 10 of the culture box and its side dams 11 are 20 centimetres, and the distance between left wall and right wall are 24 centimetres in body;The culture carriage can move left and right on the slide rail 4 of the body along slide rail 4.
Embodiment 3:
The present embodiment for the present invention bacteria cellulose fermenting and producing apparatus body and cultivate capsule together with structure example.As shown in figure 3, have in the bacteria cellulose fermenting and producing equipment of the present embodiment 10 culture boxes, wherein above 5 culture boxes be open mode place, below 5 culture boxes be closed mode placement, i.e., progress fermented and cultured when state.Dark matter in Fig. 3 culture boxes is the fluid nutrient medium 15 of different-thickness.
When the bacteria cellulose fermenting and producing equipment using the present invention carries out the production of quiescence cells cellulose fermentation, the sliding block 13 for being used to adjust liquid culture medium height on each culture box is first fixed to required position, allow each culture box according to production needs, the liquid culture medium of different height is loaded, the bacteria cellulose film of different-thickness is gone out so as to fermenting and producing.Then culture carriage is pushed into close, makes bacteria cellulose fermenting and producing equipment integral sealing on the slide rail of body.
Then, inside the first floor culture box that liquid culture medium after advance inoculation is injected into equipment from the feed pipe 6 at the top of the bacteria cellulose fermenting and producing equipment of the present invention, when the culture medium liquid level in first floor culture box, which reaches, cultivates the liquid level set by box top shoe 13, fluid nutrient medium can be flowed into from the hole 12 above sliding block in next layer of culture box.By that analogy, with the continuous injection of liquid culture medium, culture medium can from first floor culture box step by step fill up all culture boxes, now the culture medium in each culture box highly is beforehand through the different height of sliding block setting, then carries out fermented and cultured.
After fermented and cultured terminates, any one layer of culture box can be pulled out as needed from the left and right sides of bacteria cellulose fermenting and producing equipment, the collection of bacteria cellulose is carried out and culture box is cleaned.
Claims (9)
1. a kind of Zymolysis Equipment, including body and at least two culture box, wherein,
The body includes roof(1), bottom wall, antetheca(2), rear wall, left wall, right wall(3);
Wherein described antetheca(2)Multiple slide rails being parallel to each other are provided with the inner side of rear wall(4), and antetheca(2)With the slide rail on rear wall(4)It is corresponding contour;In the left wall and right wall(3)On, with upper and lower two slide rails(4)At intervals of height, be arranged in sequence with opening in opposite direction from top to bottom(5);
The culture box has a bottom surface and four side boards, described, wherein side board baffle plate(10)Height be less than or equal to the body on two slide rails up and down(4)Interval height, and provided with opening(5)The side dams of side(11)On, provided with the structure for controlling liquid culture medium height, it is preferably provided with hole(12)And sliding block(13);
Slide rail of the culture carriage in the body(4)On, can be along slide rail(4)Move left and right, preferably described culture box can be dismantled from opening.
2. Zymolysis Equipment according to claim 1, in the roof of the body(1)On be additionally provided with feed pipe(6)And gas outlet(7);Waste liquid effuser is additionally provided with the bottom wall of the body(8)And air inlet(9);It is preferred that in the antetheca of the body(2)Or temperature-adjusting device is also equipped with rear wall, furthermore it is preferred that being provided with opening in culture box(5)The side dams of side are provided with handle.
3. Zymolysis Equipment according to claim 1, wherein the height of the baffle plate of the culture box can be independently each 5-15 centimetres.
4. Zymolysis Equipment according to claim 1, wherein the hole on the culture box baffle plate(12)And sliding block(13)Any one in 1-5 groups of quantity.
5. Zymolysis Equipment according to claim 4, wherein the hole on the culture box baffle plate(12)Shape selection rectangle, ellipse, arch.
6. Zymolysis Equipment according to claim 4, wherein the hole on the culture box baffle plate(12)Around, it is additionally provided with the scale designation for showing liquid culture medium height(14).
7. Zymolysis Equipment according to claim 1, wherein the hole on the culture box baffle plate(12)Outside, it is additionally provided with flow guiding structure.
8. according to the Zymolysis Equipment any one of claim 1-7, wherein the culture box quantity in the bacteria cellulose fermenting and producing equipment is 3-100, preferably 5-60, more preferably 10-40.
9. according to the Zymolysis Equipment any one of claim 1-8, for static fermentation, it is preferred for preparing bacteria cellulose.
Priority Applications (1)
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CN201610367656.6A CN107446796B (en) | 2016-05-30 | 2016-05-30 | Bacterial cellulose fermentation production equipment |
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CN201610367656.6A CN107446796B (en) | 2016-05-30 | 2016-05-30 | Bacterial cellulose fermentation production equipment |
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CN107446796A true CN107446796A (en) | 2017-12-08 |
CN107446796B CN107446796B (en) | 2021-02-05 |
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CN201610367656.6A Expired - Fee Related CN107446796B (en) | 2016-05-30 | 2016-05-30 | Bacterial cellulose fermentation production equipment |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105950682A (en) * | 2016-05-30 | 2016-09-21 | 天津科技大学 | Fermentation method |
CN106047667A (en) * | 2016-05-30 | 2016-10-26 | 天津科技大学 | Bacterial cellulose fermentation production equipment |
CN113897404A (en) * | 2021-10-26 | 2022-01-07 | 山东纳美德生物科技有限公司 | Production device capable of precisely controlling thickness of bacterial cellulose membrane and preparation method thereof |
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CN113897404A (en) * | 2021-10-26 | 2022-01-07 | 山东纳美德生物科技有限公司 | Production device capable of precisely controlling thickness of bacterial cellulose membrane and preparation method thereof |
CN113897404B (en) * | 2021-10-26 | 2024-05-24 | 山东纳美德生物科技有限公司 | Production device capable of accurately controlling thickness of bacterial cellulose film and preparation method thereof |
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