CN113897404B - Production device capable of accurately controlling thickness of bacterial cellulose film and preparation method thereof - Google Patents
Production device capable of accurately controlling thickness of bacterial cellulose film and preparation method thereof Download PDFInfo
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- CN113897404B CN113897404B CN202111249238.4A CN202111249238A CN113897404B CN 113897404 B CN113897404 B CN 113897404B CN 202111249238 A CN202111249238 A CN 202111249238A CN 113897404 B CN113897404 B CN 113897404B
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- C—CHEMISTRY; METALLURGY
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D1/00—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor
- B26D1/01—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work
- B26D1/04—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work having a linearly-movable cutting member
- B26D1/06—Cutting through work characterised by the nature or movement of the cutting member or particular materials not otherwise provided for; Apparatus or machines therefor; Cutting members therefor involving a cutting member which does not travel with the work having a linearly-movable cutting member wherein the cutting member reciprocates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/26—Means for mounting or adjusting the cutting member; Means for adjusting the stroke of the cutting member
- B26D7/2614—Means for mounting the cutting member
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B26—HAND CUTTING TOOLS; CUTTING; SEVERING
- B26D—CUTTING; DETAILS COMMON TO MACHINES FOR PERFORATING, PUNCHING, CUTTING-OUT, STAMPING-OUT OR SEVERING
- B26D7/00—Details of apparatus for cutting, cutting-out, stamping-out, punching, perforating, or severing by means other than cutting
- B26D7/26—Means for mounting or adjusting the cutting member; Means for adjusting the stroke of the cutting member
- B26D7/2628—Means for adjusting the position of the cutting member
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/44—Multiple separable units; Modules
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention relates to the technical field of biology, in particular to a production device capable of accurately controlling the thickness of bacterial cellulose film and a preparation method thereof. In order to solve the problems of uneven surface, unsmooth, uneven density and inconsistent thickness of cut-out film produced by standing fermentation in the prior art, the invention provides a production device capable of accurately controlling the thickness of a bacterial cellulose film and a preparation method thereof.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a production device capable of accurately controlling the thickness of bacterial cellulose film and a preparation method thereof.
Background
The disclosure of this background section is only intended to increase the understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art.
Bacterial cellulose (Bacterial cellulose, BC for short) is mainly a high molecular carbohydrate synthesized by bacteria outside cells, has very similar chemical composition to natural plant cellulose, and is formed by connecting glucose with b-1, 4-glycosidic bond. The bacterial cellulose has a superfine reticular fiber structure, pure texture, high crystallinity and strong water absorption, is a natural 'sponge' of nano materials, has good biological safety and degradability, has mild synthesis process and strong film forming property, and is a 'nonwoven fabric woven by innumerable bacteria' by an image metaphor; other properties of bacterial cellulose: ultrafine property, super-strong performance, shape plasticity, water permeability, air permeability, biosynthesis controllability and the like.
The advantages indicate that the bacterial cellulose has irreplaceable application prospect in the fields of food, medical treatment, industry and the like. Fermentation is a key step for forming a film of bacterial cellulose, the strength of the bacterial cellulose is far higher than that of dynamic culture during static culture, and the current method for industrially producing the bacterial cellulose film is mostly standing fermentation, but the inventor researches and discovers that the temperature of each area of a fermentation environment is difficult to be consistent during the standing fermentation process, so that the thickness density of the fermented bacterial cellulose film is difficult to be consistent, and the bacterial cellulose has strong water absorption, the weight of the bacterial cellulose film after water absorption is increased by tens of times, the volume of the bacterial cellulose film is increased by times, the bacterial cellulose film is as fine and soft as jelly, and the thickness and the density of each film are not uniform, so that the thickness of a product in a forming stage is difficult to control, the qualification rate of the product is low, and the waste is high.
Disclosure of Invention
In order to solve the contradiction that the bacterial cellulose membrane produced by standing fermentation cannot balance thickness and density stability and the problems that the cut membrane surface is uneven, not smooth and the thickness is uncontrollable in the prior art, the invention provides a production device capable of accurately controlling the thickness of the bacterial cellulose membrane and a preparation method thereof.
Specifically, the invention is realized by the following technical scheme:
In a first aspect of the present invention, there is provided a method for preparing a bacterial cellulose film having a controlled thickness with precision, comprising the steps of: activating seeds, performing amplification culture and fermentation, and transferring the strains to a fermentation tray for static culture when the strains enter a growth logarithmic phase.
In a second aspect of the invention, a production device capable of accurately controlling the thickness of bacterial cellulose film is provided, comprising a fermentation device and a standing fermentation device;
the fermentation device is provided with a feed inlet, a vent hole and a discharge outlet;
The fermenting installation that stands includes box and fermentation tray, fermentation tray is located the box, is equipped with the feed line on the box, the feed line is connected in different fermentation trays respectively, and fermentation tray bottom surface is equipped with the rectangle baffle, and the baffle slope sets up, is connected with fermentation tray bottom through an limit, and fermentation tray is extended to the opposite side on this limit, and the lower surface of baffle is equipped with the wave line that sets up along the incline direction.
In a third aspect of the invention, a bacterial cellulose membrane prepared by a preparation method capable of accurately controlling the thickness of the bacterial cellulose membrane is provided.
The technical scheme has the following beneficial effects:
1) Through optimizing the seed liquid culture bar process, seed liquid is firstly cultured in a common fermentation tank, and then seeds in a growth logarithmic phase are transferred to a specific fermentation tray, so that a bacterial cellulose membrane with more uniform and compact density and larger thickness is easily obtained.
2) The traditional method directly carries out standing fermentation on the seed liquid, and the obtained film has the defects of bubbles and the like on the surface, and has uneven density and low density. According to the scheme, the seed liquid in the growth logarithmic phase is transferred into the specific fermentation tray through stirring, so that the mixing degree of the seed liquid is enhanced, the negative influence of transfer on the seed liquid can be reduced, and the phenomenon that the quality of the bacterial cellulose membrane after fermentation is influenced due to excessive fungus transfer loss of the seed liquid is avoided.
3) Experiments also find that the inclined baffle plate is arranged at the bottom of the fermentation tray, so that water vapor generated in the fermentation process can be drained, water drops formed by water vapor condensation can be prevented from falling back to the surface of the bacterial cellulose membrane, or water absorption layers are generated on the surface of the cellulose membrane due to overhigh water vapor, and the uniformity of the overall density is influenced.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention. Embodiments of the present invention are described in detail below with reference to the attached drawing figures, wherein:
FIG. 1 is a schematic diagram of a stationary fermentation apparatus in a production apparatus for precisely controlling the thickness of bacterial cellulose film according to example 1 of the present invention;
FIG. 2 is a schematic view of a fermentation tray and a baffle plate in a stationary fermentation apparatus according to example 1 of the present invention;
FIG. 3 is a top view of a baffle plate in a stationary fermentation apparatus according to example 1 of the present invention;
FIG. 4 is a front view of a pre-press film section in a production apparatus for precisely controlling the thickness of a bacterial cellulose film according to example 1 of the present invention;
FIG. 5 is a right side view of a pre-press film section in a production apparatus for precisely controlling the thickness of a bacterial cellulose film according to example 1 of the present invention;
FIG. 6 is a schematic view of a blade clamping groove in a production device capable of precisely controlling the thickness of bacterial cellulose film according to example 1 of the present invention;
FIG. 7 is a view showing the appearance of a bacterial cellulose membrane prepared by the conventional method and example 1 of the present invention;
FIG. 8 is an external view showing the bacterial cellulose membrane prepared in comparative example 1 of the present invention;
FIG. 9 is an external view showing a bacterial cellulose membrane prepared in comparative example 2 of the present invention;
FIG. 10 is an external view showing a bacterial cellulose membrane prepared in comparative example 3 of the present invention;
FIG. 11 is a graph showing the properties of bacterial cellulose membranes prepared in example 2 and comparative examples 1-3 of the present invention, (a) example 2, (b) comparative example 1, (c) comparative example 2, and (d) comparative example 3;
Wherein: 1. exhaust fan, 2, control console, 3, glass, 4, feed line, 5, solenoid valve, 6, fermentation tray, 7, ultraviolet lamp and lighting lamp tube, 8, air inlet fan, 9, baffle, 10, bracket, 11, long conveyer belt, 12, short conveyer belt, 13, electric lifting rod, 14, knife protecting shell, 15, support frame, 16, knife blade draw-in groove, 17, knife rest, 18, round hole, 19, draw-in groove.
Detailed Description
The invention will be further illustrated with reference to specific examples. It is to be understood that these examples are illustrative of the present invention and are not intended to limit the scope of the present invention. The experimental procedures, which do not address the specific conditions in the examples below, are generally carried out under conventional conditions or under conditions recommended by the manufacturer.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments in accordance with the present disclosure. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
It should be understood that the terms "upper," "lower," "horizontal," "top," "bottom," and the like indicate or relate to a position or location based on the position or location shown in the drawings, and are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element in question must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.
In order to solve the problems of inconsistent film thickness, uneven density, uneven surface of a cut film with high difficulty in controlling thickness and unsmooth surface of the bacterial cellulose film produced by standing fermentation in the prior art, the invention provides a production device capable of accurately controlling the film thickness of the bacterial cellulose film and a preparation method thereof.
Specifically, the invention is realized by the following technical scheme:
In a first aspect of the present invention, there is provided a method for preparing a bacterial cellulose film having a controlled thickness with precision, comprising the steps of: activating seeds, performing amplification culture, fermenting, transferring the strains to a fermentation tray for static culture when the strains enter a growth log phase.
Through optimizing the seed liquid culture bar process, seed liquid is firstly cultured in a common fermentation tank, and then seeds in a growth logarithmic phase are transferred to a specific fermentation tray, so that a bacterial cellulose membrane with more uniform and compact density and larger thickness is easily obtained.
The traditional method directly carries out standing fermentation on the seed liquid, and the obtained film surface is influenced by condensed water and has the defects of bubbles and the like, and has uneven density and low density. According to the scheme, the seed liquid in the growth logarithmic phase is transferred into the specific fermentation tray through stirring, so that the mixing degree of the seed liquid is enhanced, the negative influence of transfer on the seed liquid can be reduced, and the phenomenon that the quality of the bacterial cellulose membrane after fermentation is influenced due to excessive fungus transfer loss of the seed liquid is avoided.
In one or more embodiments of the present invention, the strain liquid is kept in a stirred and mixed state during the transfer process.
In one or more embodiments of the invention, the stationary culture process prevents water vapor generated by the strain from condensing into water drops to the fermentation tray.
In one or more embodiments of the present invention, the seed is selected from at least one of acetobacter xylinum, acetobacter pasteurium, acetobacter hanothei, acetobacter gluconicum, agrobacterium, acetobacter aceti, achromobacter, enterobacter, pseudomonas, azotobacter, rhizobium, sarcina, campylobacter, salmonella, escherichia;
Preferably, the activated seed comprises: preparing seed liquid, sterilizing by high-pressure steam, culturing for a period of time, and inoculating to obtain first-stage seed liquid;
Preferably, the expanding culture comprises the steps of inoculating the primary seed liquid into the seed liquid of the fermentation tank, wherein the seed quantity is 6% -10%, the rotating speed is 50% -100 r/min, and the secondary seed liquid is obtained after the primary seed liquid is cultured for a period of time.
In one or more embodiments of the invention, the fermenting step includes introducing the secondary seed liquid into a fermenting apparatus with an inoculum size of 6% -10%, continuing to culture in the fermenting apparatus, and controlling aeration and dissolved oxygen saturation.
In one or more embodiments of the invention, the transfer process includes: when the strain enters into the growth logarithmic phase or starts fermenting for 4-12 h from the strain entering into the fermentation device, the fermentation liquid is transferred into the fermentation container through the pipeline at the rotating speed of 50-100 r/min for 5-10 cm in depth, and the stationary culture is started.
In some more specific embodiments, the method of making comprises:
(1) Activating seeds, preparing seed liquid, sterilizing by high-pressure steam at 115-126 ℃, culturing for 16-24 hours, and inoculating to obtain primary seed liquid, wherein the condition of the primary seed liquid culture is 28-32 ℃; the seed can be at least one of Acetobacter xylinum, acetobacter pasteurii, acetobacter hancei, gluconobacter, agrobacterium, acetobacter aceti, achromobacter, enterobacter, pseudomonas, azotobacter, rhizobium, sarcina, campylobacter, salmonella, and Escherichia.
(2) Performing expansion culture, namely inoculating the first-stage seed liquid obtained in the step 1 into the seed liquid of the fermentation tank, wherein the seed quantity is 6% -10%, the rotating speed is 50-100 r/min, and culturing for 12-20 hours to obtain a second-stage seed liquid;
(3) The second-level seed liquid in the step (2) is inoculated into an air-opened fermentation tank, the inoculation amount is 6% -10%, the culture is continued in the fermentation tank, the temperature is 28-32 ℃, the pH is 5-6, the rotating speed is 50-100 r/min, the ventilation amount is 0.08-0.15 m < lambda > (- ∙ h), and the dissolved oxygen saturation is more than or equal to 95%;
(4) Culturing in an air-open fermentation tank for 4-12 hours, enabling strains to enter a growth log phase, enabling the bacterial growth speed to reach the maximum at the moment, keeping the rotation speed to be 50-100 r/min, transferring fermentation liquor into a fermentation container through a pipeline to a depth of 5-10 cm, and starting stationary culture; the purpose is as follows: the bacteria can adapt to the fermentation environment more quickly after being cultured in the fermentation tank to the logarithmic phase with the fastest growth speed of the bacteria, and the bacteria are uniformly distributed in the fermentation tray in higher concentration, so that the density uniformity of bacterial cellulose membranes in the later stage can be ensured.
(5) Observing the consumption of the fermentation liquid during fermentation, and continuously supplementing the fermentation liquid into a fermentation tray through fermentation equipment to maintain the depth of the fermentation liquid at 5-10 cm; the purpose is as follows: the raw material consumption caused by the high-speed growth of bacteria is supplemented, the nutrition of substances in the fermentation process is ensured, the growth of acetobacter xylinum is promoted, the sugar conversion rate and the average bacterial growth rate reach higher growth level, bacterial cellulose is converted and synthesized in a large amount, the depth of fermentation liquor is maintained, and the thickness of bacterial cellulose film is increased.
(6) Adding a surfactant Tw-80 at 36h from the beginning of fermentation, wherein the dosage is 0.02-0.04%; the surfactant can change the permeability of cell membrane, is favorable for the uptake of nutrition and release of products by cells, has certain promotion effects on enzyme production, polysaccharide production and the like of microorganisms in fermentation, generates more cellulose, and can make the membrane thicker.
(7) The duration of the fermentation process is 7-8 days, the surface of the membrane after the fermentation is smooth, and the membrane is taken out from the fermentation tray;
(8) And pre-pressing the cellulose membrane after fermentation into a membrane with uniform thickness and compact texture, then flatly cutting the membrane into at least two membranes with specific thickness, regulating the distance between the blades through a flatly cutting device to obtain the thickness which is not more than the thickness of the cellulose membrane, and smoothing and flattening the two surfaces of the flatly cut membrane, wherein the flatly cut membrane can be used for producing products in other fields after treatment.
By optimizing the seed liquid culture bar process, bacterial seeds with higher activity are obtained, bacterial growth in a fermentation tray is faster, and a film with more uniform density is easily obtained; optimizing the fermentation process enables the bacteria to ingest more nutrients, thereby producing more cellulose and obtaining thicker bacterial cellulose films.
In a second aspect of the invention, a production device capable of accurately controlling the thickness of bacterial cellulose film is provided, comprising a fermentation device and a standing fermentation device;
the fermentation device is provided with a feed inlet, a vent hole and a discharge outlet;
The fermenting installation that stands includes box and fermentation tray, fermentation tray is located the box, is equipped with the feed line on the box, the feed line is connected in different fermentation trays respectively, and fermentation tray bottom surface is equipped with the rectangle baffle, and the baffle slope sets up, is connected with fermentation tray bottom through an limit, and fermentation tray is extended to the opposite side on this limit, and the lower surface of baffle is equipped with the wave line that sets up along the incline direction.
In some more specific embodiments, the apparatus comprises:
The new fermentation equipment consists of a box body and a plurality of fermentation trays inside the box body, and the outer surface of the equipment is formed by splicing stainless steel.
The box comprises a left side face, a right side face, a top face, a bottom face, a rear face and a front face (door) 6 part, the front portion of the box is connected with equipment through a hinge, sealing gaskets are attached to four sides of the front face, sealing gaskets are attached to corresponding frames of the front face of the equipment, after the front face is closed, the equipment is sealed well, and the equipment after disinfection cannot be contacted with the outside due to good sealing performance, so that pollution is avoided.
The control console consists of a touchable screen and various controllers, can display the information of the temperature and humidity, fermentation time, fermentation liquid depth and the like in the current fermentation vehicle, and can control the opening and closing of an inlet fan, an exhaust fan, an electromagnetic valve, an ultraviolet lamp and an illuminating lamp, and control the start and the end of fermentation; fermentation information can be read through the USB flash disk.
The ultraviolet lamp and the lighting lamp tube are respectively connected at the top and the bottom of the equipment through the tube seat, the ultraviolet lamp can disinfect the interior of the fermentation equipment after the fermentation is finished and the cleaning is finished, the lighting lamp is used for lighting, and the ultraviolet lamp is matched with the disinfectant to enable the equipment to be thoroughly disinfected, so that pollution is avoided.
The feeding pipeline is used for conveying fermentation raw materials from the outside of the equipment to the inside of the equipment, and branches leading to the fermentation tray are left after entering the equipment, the branches are provided with holes above the fermentation tray, and electromagnetic valves are arranged on the branches, so that the fermentation tray can be taken out to be added with the raw materials, the raw materials are prevented from contacting with the outside, the pollution is reduced, and the fermentation process is safer.
And each fermentation tray is internally provided with a liquid level meter, the liquid level meter is connected with equipment through a quick connector, the equipment is convenient to detach and replace, the depth of the fermentation liquid can be sensed and fed back to the control console to display the depth of the fermentation liquid at the moment, and when the residual fermentation liquid reaches a preset value, the control console can control the electromagnetic valve to switch to supplement the consumed fermentation liquid, so that the depth of the fermentation liquid is maintained. The fermentation tray is internally provided with fermentation liquor, and transparent materials are convenient for observing the residual fermentation liquor and the thickness of the cellulose film, so that the fermentation tray is a place for producing the film by bacterial growth; the middle of the device is provided with the bracket, and the fermentation tray is placed on the bracket, so that the fermentation tray is more convenient to pull and place.
The bottom surface of the fermentation tray is provided with a corrugated plate with a special structure, the long side of the corrugated plate is identical to the long side of the bottom surface of the fermentation tray, the wide side of the corrugated plate is longer than the wide side of the bottom surface of the fermentation tray, two short sides of the corrugated plate are clamped in clamping grooves corresponding to the fermentation equipment, an angle of 5 degrees is formed between the corrugated plate and the bottom surface of the fermentation tray, the other side of the corrugated plate exceeds the bottom surface of the fermentation tray, and the downward side of the corrugated plate is provided with a wave-shaped groove in the direction of the extending short side, so that condensed water formed in the fermentation process can be gathered and flows out of the fermentation tray through an inclined plane, and finally gathered into a condensed water collecting tray at the bottom of the fermentation equipment, because condensed water drops on a bacterial cellulose film in the fermentation, cellulose can grow towards the water drops to lead to protrusion on the surface of the film to form pimple, and the Mole surface is uneven, and the condensed water is guided into the bottom collecting tray to avoid the occurrence of the situation.
The contact surface between the exhaust fan and the inside of the equipment is covered by an efficient filter screen, when the temperature in the automobile is too high, the temperature can be reduced through ventilation, meanwhile, the oxygen content in the equipment is increased by matching with the intake fan, and the exhaust fan is fixed at the top of the equipment through 4 screws; the inlet fan is covered by the high-efficiency filter screen by the external contact surface, external dust bacteria are prevented from entering the device, the inlet fan is fixed below the right side part of the device through 4 screws, the six surfaces inside the fermentation device are also provided with temperature sensing and heating pipes, the temperature sensing and heating pipes cooperate with the inlet fan and the exhaust fan to stabilize the oxygen content and the temperature in the device, so that the temperature of each area in the fermentation device is consistent, and the temperature is displayed on a control console.
The bottom of the fermentation equipment is supported by four rollers on the ground, so that the fermentation equipment can freely control the direction to move at will in the moving process, and the labor is saved.
In one or more embodiments of the invention, the inner wall of the box body is provided with at least one groove which is arranged in parallel, the height of the groove is matched with the height of the fermentation tray, so that the fermentation tray can submerge in the groove, or a bracket is arranged in the box body, and the fermentation tray is positioned on the bracket.
In one or more embodiments of the present invention, the slicing device further includes a control console, a pre-pressing film area and an arc-shaped cutter, the control console is used for controlling slice thickness, the pre-pressing film area is provided with two groups of conveyor belts, each group of conveyor belts includes an upper conveyor belt and a lower conveyor belt, the upper conveyor belts of the two groups of conveyor belts are the same in height, the lower conveyor belts are the same in height, the length of the arc-shaped cutter is smaller than the width of the conveyor belts, the arc-shaped cutter is horizontally placed between the two groups of conveyor belts, and the length of the arc-shaped cutter is perpendicular to the length direction of the conveyor belts.
In some more specific embodiments, the trimming device comprises:
A control console, a pre-pressing film area and an arc-shaped cutter row.
The pre-pressing film area mainly comprises two conveyor belts, an arc-shaped row cutter and four electric lifting rods; the main part of equipment is two parallel conveyer belts, and the higher authority is the short conveyer belt, and the lower authority is the long conveyer belt, and the four corners of short conveyer belt is by screwed connection electric lift pole, and electric lift pole passes through the screw fixation on the frame of long conveyer belt, and four electric lift poles pass through the controller and are connected with the control cabinet, make the control cabinet can remove the distance between two conveyer belts of short conveyer belt control. When the bacterial cellulose membrane passes through the area, a part of water is lost by the extrusion of the two conveyor belts, so that the bacterial cellulose membrane becomes more compact, and simultaneously the two conveyor belts provide friction force to enable the membrane to pass through the arc-shaped row cutters quickly, so that the cut membrane is smoother; the electric lifting rod controlled by the computer is simple and convenient to adjust, and the precision is higher.
The cutter row consists of a plurality of arc-shaped blades, a cutter rest, a plurality of groups of blade clamping grooves and a plurality of groups of electric lifting rods, wherein two blade clamping grooves and two electric lifting rods are needed for 1 arc-shaped blade; the knife rest is two stainless steel pipes, the lower ends of the knife rest are fixed on the frame of the long conveyor belt, the two sides of the knife rest are symmetrical, and the upper ends of the knife rest are higher than the short conveyor belt; the blade clamping grooves are provided with round holes, the diameters of the round holes are matched with the diameters of the tool rest, the blade clamping grooves can be arranged on the tool rest through the round holes, each group of clamping grooves are symmetrically arranged left and right, and an arc-shaped blade is controlled in the middle of each group of clamping grooves; each clamping groove is connected with an electric lifting rod through a long screw, and the electric lifting rod is connected with a control console through a controller; the control console controls the expansion and contraction of the two electric lifting rods to move a group of blade clamping grooves, so that the movement of the arc-shaped blades is realized, the distance between the blades is adjusted, and the thickness of a film is accurately controlled; the part of the upper end of the tool rest, which is higher than the upper end of the tool rest, is used for placing a temporarily unavailable blade clamping groove.
The control console is arranged beside the equipment, and has the main functions of controlling the switch of the equipment, adjusting the speed of the conveyor belt, adjusting the distance between the short conveyor belt and the long conveyor belt and adjusting the distance between the blades.
In a third aspect of the invention, a bacterial cellulose membrane prepared by a preparation method capable of accurately controlling the thickness of the bacterial cellulose membrane is provided.
The invention will now be described in further detail with reference to the following specific examples, which should be construed as illustrative rather than limiting.
Example 1
(1) Preparing a solid culture medium, and activating seed acetobacter xylinum after sterilizing at the high temperature of 121 ℃;
(2) Preparing seed liquid, inoculating after shaking culture for 24h to obtain primary seed liquid, wherein the primary seed liquid is cultured at 30 ℃ and 160r/min;
(3) Performing expansion culture, namely inoculating the primary seed liquid obtained in the step (2) into the seed liquid of the fermentation tank, wherein the seed quantity is 10%, the rotating speed is 50r/min, and culturing for 20 hours to obtain a secondary seed liquid;
(4) The second-level seed liquid in the step 2 is inoculated into an air-opened fermentation tank, the inoculation amount is 10 percent, the culture is continued in the fermentation tank, the temperature is 30 ℃, the pH is 6.0, the rotating speed is 100r/min, the ventilation amount is 0.08-0.15 mW/(- ∙ h), and the dissolved oxygen saturation is more than or equal to 95 percent;
(5) When the strain enters a growth logarithmic phase and fermentation starts for 12h, maintaining the rotating speed at 100r/min;
(6) Pre-sterilizing a fermentation tray by using a sterilizing liquid and an ultraviolet lamp, wherein scale marks are arranged in the fermentation tray, transferring the fermentation liquid into the fermentation tray to a depth of 5cm, and starting standing fermentation;
(7) The depth of the fermentation liquid is frequently observed during fermentation, and the fermentation liquid is supplemented into the fermentation tray every time the consumption of the fermentation liquid exceeds 0.5cm, so that the depth of the fermentation liquid is maintained at 5cm;
(8) Adding surfactant Tw-80 at 36h from the beginning of fermentation, wherein the dosage is 0.04%, and adding into a fermentation tray by using a pipette;
(9) The duration of the fermentation process is 8 days, the surface of the film after the fermentation is smooth, the texture is compact, the water content is 96-98%, and the thickness is 3.2cm on average;
(10) The bacterial cellulose film was taken out and subjected to flat cut into 8 pieces of 3mm cellulose film.
Bacterial cellulose films with uniform thickness density can be obtained by optimizing the fermentation process, and the thickness is enough for a plurality of products with various types to be cut flatly.
The using method of the static fermentation device comprises the following steps:
As shown in fig. 1 and 2, the console 2 is composed of a touchable screen, and the information of the temperature, fermentation time, fermentation liquid depth and the like in the current fermentation equipment is displayed on the touch screen, so that the switches of the inlet fan 8, the exhaust fan 1, the electromagnetic valve 5, the ultraviolet lamp and the lighting lamp tube 7 can be controlled, and the start and the end of fermentation can be regulated and controlled; and the information during fermentation can be read through the USB flash disk, and the data can be analyzed to provide various data.
The fermentation equipment consists of a box body and a plurality of fermentation trays 6, and the interior of the equipment is well sealed after the front surface of the equipment is closed, so that pollution is reduced; the top surface of the equipment is provided with an exhaust fan 1 and a feed inlet, the contact surface between the exhaust fan 1 and the inside of the equipment is covered by a high-efficiency filter screen, so that the air in the equipment can be discharged, the right side surface of the equipment is provided with an inlet fan 8, the contact surface between the inlet fan 8 and the outside is covered by the high-efficiency filter screen, and six surfaces of the fermentation equipment are also provided with a temperature probe and a heating pipe; after the fermentation temperature is set, the temperature probe senses the temperature and feeds back to the control console, and the air inlet fan 8, the exhaust fan 1 and the heating pipe work together to enable hot air to circulate in the equipment, so that the temperature of each area in the fermentation equipment is consistent, bacteria in each area grow consistently, and bacterial cellulose films with uniform density and consistent thickness are produced; the bottom surface of the device is provided with a collecting tray for collecting condensed water.
The ultraviolet lamp and the lighting lamp tube 7 can disinfect the interior of the fermentation equipment after the fermentation is finished and the cleaning is finished, the lighting lamp is used for lighting, and the ultraviolet lamp is matched with the disinfectant to thoroughly disinfect the equipment, so that pollution is avoided.
The feeding pipeline 4 is used for conveying fermentation raw materials from the outside of the equipment to the inside of the equipment, a branch leading to the fermentation tray 6 is arranged after the fermentation raw materials enter the equipment, the opening is arranged above the fermentation tray 6, the solenoid valve 5 is arranged on the branch, a system control switch is used for setting a feeding rule, and fermentation liquid can be automatically added after the feeding rule is set by a control console, so that the raw materials are prevented from contacting the outside, and pollution is reduced.
A liquid level meter is arranged in the fermentation tray 6, the depth of the fermentation liquid is displayed in real time and is fed back to a control console, so that the switch of the electromagnetic valve 5 is controlled; the bottom surface of the fermentation tray 6 is provided with an inclined baffle plate 9, so that condensed water generated in the fermentation process can be guided out of the fermentation tray 6 and flows into a collecting tray at the bottom surface; the fermentation tray 6 is clamped on the bracket 10 through the inclined plate, so that the fermentation tray is more stable.
The bottom of the fermentation equipment is supported by four rollers on the ground, so that the fermentation equipment can freely control the direction to move at will in the moving process, and the labor is saved.
The using method of the film cutting device comprises the following steps:
As shown in fig. 3, 4,5 and 6, the console is arranged beside the device and has the main functions of controlling the switch of the device, the speed of the conveyor belt, adjusting the distance between the short conveyor belt and the long conveyor belt and adjusting the distance between the blades. The bacterial cellulose membrane loses some water through a pre-pressing membrane area, and is cut and formed through a row cutter after becoming compact.
The pre-pressing film area mainly comprises two conveyor belts, an arc-shaped row cutter and four electric lifting rods; two parallel conveyor belts are arranged on the supporting frame 15, a short conveyor belt 12 is arranged on the upper side, a long conveyor belt 11 is arranged on the lower side, and four electric lifting rods 13 are connected with a control console through a controller, so that the control console can move the short conveyor belt to control the distance between the two conveyor belts by adjusting the four electric lifting rods 13. When the bacterial cellulose membrane passes through the area, a part of water is lost by the extrusion of the two conveyor belts, so that the bacterial cellulose membrane becomes more compact, and simultaneously the two conveyor belts provide friction force to enable the membrane to pass through the arc-shaped row cutters quickly, so that the cut membrane is smoother; the electric lifting rod controlled by the computer is simple and convenient to adjust, and the precision is higher.
After the bacterial cellulose film is pre-pressed in the front, the bacterial cellulose film comes to a cutter arranging position, the cutter arranging position is composed of a plurality of arc-shaped blades, a cutter rest 17, a plurality of groups of blade clamping grooves 16 and a plurality of groups of electric lifting rods, round holes 18 are formed in the blade clamping grooves 19, grooves are formed in the cutter rest 17 and are matched with the round holes, and the round holes 18 are fixed on the grooves when being inserted into the cutter rest 17. The row of knives is provided with a knife protecting shell 14, improving safety.
The arc-shaped blade can be detached by the blade buckle 19, so that the replacement is convenient; the electric lifting rod is connected with the control console through the controller; the control console controls the extension and retraction of the two electric lifting rods to move a group of blade clamping grooves 16, so that the movement of the arc-shaped blades is realized, the distance between the blades is adjusted, and the thickness of a film is accurately controlled; the part of the upper end of the tool rest, which is higher than the upper end of the tool rest, is used for placing a temporarily unavailable blade clamping groove.
As shown in fig. 7, (a) (b) is a cellulose membrane obtained by the conventional fermentation method for 7 days, and (c) (d) is a cellulose membrane obtained by the method of this example. (a) (b) the film surface is loose, the fingerprints are left, the thickness is thinner, and (c) the film thickness is thicker, the color and luster are white, the accumulation state of the cellulose film can be seen from the cross section, and the texture is compact.
Experimental example 2
(1) Activating seeds, preparing seed liquid, sterilizing with high-pressure steam at 121 ℃, culturing for 24 hours, and inoculating to obtain primary seed liquid, wherein the temperature of the primary seed liquid is 30 ℃ during culturing; the seed is acetobacter xylinum.
(2) Performing expansion culture, namely inoculating the first-stage seed liquid obtained in the step ⑴ into the seed liquid of the fermentation tank, wherein the seed quantity is 10%, the rotating speed is 100r/min, and culturing for 20 hours to obtain a second-stage seed liquid;
(3) The second-level seed liquid in the step (2) is inoculated into an air-opened fermentation tank, the inoculum size is 10 percent, the culture is continued in the fermentation tank, the temperature is 30 ℃, the pH is 5-6, the rotating speed is 70r/min, the ventilation is 0.08-0.15 mW/(- ∙ h), and the saturation of dissolved oxygen is more than or equal to 95 percent;
(4) In the 6 th hour of the culture in the gas-open type fermentation tank, the strain enters a growth logarithmic phase, at the moment, the growth speed of bacteria reaches the maximum, the bacteria can adapt to the fermentation environment more quickly, the rotation speed is kept at 70r/min, the fermentation liquid is transferred to the depth of 5cm in the fermentation container through a pipeline, the same number of thalli in each fermentation container is ensured, the later growth is more uniform, and the stationary culture is started;
(5) The consumption of fermentation liquor is observed during fermentation, fermentation liquor is continuously supplemented into a fermentation tray through fermentation equipment, so that the depth of the fermentation liquor is maintained at 5cm, raw material consumption caused by high-speed growth of bacteria is supplemented, sufficient nutrition of substances in the fermentation process is ensured, the growth of acetobacter xylinum is promoted, the sugar conversion rate and the average bacterial growth rate reach higher growth level, bacterial cellulose is greatly converted and synthesized, the depth of the fermentation liquor is maintained, and the growth space is maintained.
(6) Adding a surfactant Tw-80 at 36h from the beginning of fermentation, wherein the dosage is 0.02%; the surfactant can change the permeability of cell membrane, is favorable for the uptake of nutrition and release of products by cells, has certain promotion effect on enzyme production, polysaccharide production and the like of microorganisms in fermentation, and produces more cellulose.
(7) The duration of the fermentation process is 7 days, the surface of the membrane after the fermentation is smooth, the thickness is between 2.8 and 4cm, and the membrane is taken out from the fermentation container;
(8) Taking 5 pieces of 5cm x 1cm from the intersection points of four corners and diagonal lines of the cellulose membrane after fermentation at random, measuring the weight of the cellulose membrane as wet weight, evaporating the 5 pieces of membrane to dryness until the water content is constant, and measuring the weight of the cellulose membrane as dry weight.
TABLE 1 Property parameters of 5 Membrane blocks of cellulose Membrane prepared in EXAMPLE 2
Comparative example 1
(1) Activating seeds, preparing seed liquid, sterilizing with high-pressure steam at 121 ℃, culturing for 24 hours, and inoculating to obtain primary seed liquid, wherein the temperature of the primary seed liquid is 30 ℃ during culturing; the seed is acetobacter xylinum.
(2) Performing expansion culture, namely inoculating the first-stage seed liquid obtained in the step ⑴ into the seed liquid of the fermentation tank, wherein the seed quantity is 10%, the rotating speed is 100r/min, and culturing for 20 hours to obtain a second-stage seed liquid;
(3) The second-level seed liquid in the step (2) is inoculated into an air-opened fermentation tank, the inoculum size is 10 percent, the culture is continued in the fermentation tank, the temperature is 30 ℃, the pH is 5-6, the rotating speed is 70r/min, the ventilation is 0.08-0.15 mW/(- ∙ h), and the saturation of dissolved oxygen is more than or equal to 95 percent;
(4) In the 20 th hour of the culture in the gas-open type fermentation tank, bacteria grow into a declining period, the rotation speed is kept at 70r/min, fermentation liquor is transferred into the fermentation containers through pipelines to a depth of 5cm, the same number of thalli in each fermentation container is ensured, the later growth is more uniform, and the stationary culture is started;
(5) The consumption of fermentation liquor is observed during fermentation, fermentation liquor is continuously supplemented into a fermentation tray through fermentation equipment, so that the depth of the fermentation liquor is maintained at 5cm, raw material consumption caused by high-speed growth of bacteria is supplemented, sufficient nutrition of substances in the fermentation process is ensured, the growth of acetobacter xylinum is promoted, the sugar conversion rate and the average bacterial growth rate reach higher growth level, bacterial cellulose is greatly converted and synthesized, the depth of the fermentation liquor is maintained, and the growth space is maintained.
(6) Adding a surfactant Tw-80 at 36h from the beginning of fermentation, wherein the dosage is 0.02%; the surfactant can change the permeability of cell membrane, is favorable for the uptake of nutrition and release of products by cells, has certain promotion effect on enzyme production, polysaccharide production and the like of microorganisms in fermentation, and produces more cellulose.
(7) The duration of the fermentation process is 7 days, the texture of the membrane after the fermentation is loose, the thickness is about 2cm, and the membrane is taken out from the fermentation container;
(8) Taking 5 pieces of 5cm x 1cm from the intersection points of four corners and diagonal lines of the cellulose membrane after fermentation at random, measuring the weight of the cellulose membrane as wet weight, evaporating the 5 pieces of membrane to dryness until the water content is constant, and measuring the weight of the cellulose membrane as dry weight.
TABLE 2 Property parameters of 5 Membrane blocks of cellulose Membrane prepared in comparative example 1
Comparative example 2
(1) Activating seeds, preparing seed liquid, sterilizing with high-pressure steam at 121 ℃, culturing for 24 hours, and inoculating to obtain primary seed liquid, wherein the temperature of the primary seed liquid is 30 ℃ during culturing; the seed is acetobacter xylinum.
(2) Performing expansion culture, namely inoculating the first-stage seed liquid obtained in the step ⑴ into the seed liquid of the fermentation tank, wherein the seed quantity is 10%, the rotating speed is 100r/min, and culturing for 20 hours to obtain a second-stage seed liquid;
(3) The second-level seed liquid in the step (2) is inoculated into an air-opened fermentation tank, the inoculum size is 10 percent, the culture is continued in the fermentation tank, the temperature is 30 ℃, the pH is 5-6, the rotating speed is 70r/min, the ventilation is 0.08-0.15 mW/(- ∙ h), and the saturation of dissolved oxygen is more than or equal to 95 percent;
(4) In the 6 th hour of culture in the gas-open type fermentation tank, the strain enters a growth logarithmic phase, the rotation speed is kept at 70r/min, the fermentation liquid is transferred into the fermentation containers through pipelines to a depth of 5cm, the same number of thalli in each fermentation container is ensured, the later growth is more uniform, and the stationary culture is started;
(5) Fermentation broth is not supplemented during fermentation;
(6) The surfactant Tw-80 was added at 36h from the start of fermentation at a dose of 0.02%.
(7) The duration of the fermentation process is 7 days, the surface of the membrane after the fermentation is smooth, the thickness is between 1 cm and 2cm, and the membrane is taken out from the fermentation container;
(8) Taking 5 pieces of 5cm x 1cm from the intersection points of four corners and diagonal lines of the cellulose membrane after fermentation at random, measuring the weight of the cellulose membrane as wet weight, evaporating the 5 pieces of membrane to dryness until the water content is constant, and measuring the weight of the cellulose membrane as dry weight.
TABLE 3 Property parameters of 5 Membrane blocks of cellulose Membrane prepared in comparative example 2
Comparative example 3
(1) Activating seeds, preparing seed liquid, sterilizing with high-pressure steam at 121 ℃, culturing for 24 hours, and inoculating to obtain primary seed liquid, wherein the temperature of the primary seed liquid is 30 ℃ during culturing; the seed is acetobacter xylinum.
(2) Performing expansion culture, namely inoculating the first-stage seed liquid obtained in the step ⑴ into the seed liquid of the fermentation tank, wherein the seed quantity is 5%, the rotating speed is 100r/min, and culturing for 20 hours to obtain a second-stage seed liquid;
(3) The second-level seed liquid in the step (2) is inoculated into an air-opened fermentation tank, the inoculum size is 5 percent, the culture is continued in the fermentation tank, the temperature is 30 ℃, the pH is 5-6, the ventilation is 0.08-0.15 m-2 m-line/(.e. ∙ h), the dissolved oxygen saturation is more than or equal to 95 percent, and the stirring is not carried out;
(4) Transferring the fermentation liquor to a fermentation container through a pipeline for 5cm deep in the 6 th hour of culture in the gas-open fermentation tank, and starting stationary culture;
(5) And observing the consumption of the fermentation liquid during fermentation, continuously supplementing the fermentation liquid into the fermentation tray through fermentation equipment, keeping the depth of the fermentation liquid at 5cm, and supplementing the raw material consumption caused by high-speed growth of bacteria to maintain a growth space.
(6) Surfactant Tw-80 was added at 36h at the beginning of fermentation at a dose of 0.02% to produce more cellulose.
(7) The duration of the fermentation process is 7 days, the surface texture of the membrane after the fermentation is loose, the thickness is between 0.8 cm and 2.3cm, and the membrane is taken out from the fermentation container;
(8) Taking 5 pieces of 5cm x 1cm from the intersection points of four corners and diagonal lines of the cellulose membrane after fermentation at random, measuring the weight of the cellulose membrane as wet weight, evaporating the 5 pieces of membrane to dryness until the water content is constant, and measuring the weight of the cellulose membrane as dry weight.
TABLE 4 Property parameters of 5 Membrane blocks of cellulose Membrane prepared in comparative example 3
From the experimental data in table 1, it can be seen that:
The standard deviation of experimental example 2 calculated by the formula is 0.0875, 0.0086 and 0.0021 respectively, and the data fluctuation is small, namely the density of each region of the film is uniform.
As shown in fig. 8, in comparative example 1, the culture time of the secondary seeds was prolonged, the seeds grew into the decay phase, the vigor of the seeds was insufficient, the adaptation to the environment was poor, the growth was slow, and the culture time was too long, the larger cellulose masses formed in the fermenter could not be scattered, and the distribution of bacteria was uneven, thus resulting in a thin film with loose texture.
As shown in fig. 9, in comparative example 2, the fermentation process was not fed, and since the early growth of seeds consumed a lot of nutrients, the fermentation broth was gradually consumed, and the growth of bacteria was stopped in advance due to lack of nutrients, resulting in a cellulose film with a generally thinner thickness but a more compact texture.
As shown in FIG. 10, in comparative example 3, the inoculation amount was reduced to 5%, resulting in an extended bacterial growth period, and after the bacteria had entered the high-speed stage of growth, the bacteria had not been able to consume the nutrients in the fermentation broth before the end of fermentation, so that the bacteria had grown slowly, and the film had grown slowly and was thin.
As shown in fig. 11, the experimental parameters of example 2 and comparative example are compared.
It follows that the thickness of the cellulose membrane is affected by the activity of the bacterial species, the nutrients, and the bacterial species activity affects the number and concentration of bacteria, thereby affecting the thickness and density of the cellulose membrane.
The foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. The preparation method capable of accurately controlling the thickness of the bacterial cellulose film is characterized by comprising the following steps: activating seeds, performing amplification culture, fermenting, and transferring the seeds to a fermentation tray for static culture when strains enter a growth log phase;
The activated seed comprises: preparing seed liquid, sterilizing by high-pressure steam, culturing for a period of time, and inoculating to obtain first-stage seed liquid; the seed is at least one selected from Acetobacter xylinum, acetobacter pasteurii, acetobacter hancei, gluconobacter, agrobacterium, acetobacter aceti, achromobacter, enterobacter, pseudomonas, azotobacter, rhizobium, sarcina, campylobacter, salmonella, and Escherichia;
The expansion culture comprises the steps of inoculating a first-stage seed liquid into a seed liquid of a fermentation tank, wherein the inoculum size is 6% -10%, the rotating speed is 50-100 r/min, and culturing for a period of time to obtain a second-stage seed liquid;
The fermentation step comprises the steps of inoculating the secondary seed liquid into a fermentation device, wherein the inoculation amount is 6% -10%, continuously culturing in the fermentation device, and controlling ventilation and dissolved oxygen saturation;
The transfer process includes: when the strain enters a growth logarithmic phase or starts fermenting for 6-12 h from the moment that the strain enters a fermentation device, maintaining the rotating speed at 50-100 r/min, transferring the fermentation liquor into a fermentation container through a pipeline to a depth of 5-10 cm, and starting stationary culture; continuously supplementing fermentation liquor into a fermentation tray through fermentation equipment during fermentation, so that the depth of the fermentation liquor is maintained at 5-10 cm;
the strain liquid is kept in a stirring and mixing state in the transferring process;
The surface active agent Tw-80 is added in the static culture, the dosage is 0.02-0.04%, and the culture time is 7-8 days;
During the stationary culture process, the water vapor generated by the strain is prevented from being condensed into water drops to the fermentation tray;
the production device adopted by the preparation method capable of controlling the thickness of the bacterial cellulose film accurately comprises a fermentation device and a standing fermentation device;
the fermentation device is provided with a feed inlet, a vent hole and a discharge outlet;
the static fermentation device comprises a box body and a fermentation tray;
The inner wall of the box body is provided with at least one groove which is arranged in parallel, the height of the groove is matched with the height of the fermentation tray, so that the fermentation tray can submerge in the groove, or a bracket is arranged in the box body, and the fermentation tray is positioned on the bracket;
The fermentation tray is located the box, is equipped with the feed line on the box, the feed line is connected with different fermentation trays respectively, and fermentation tray bottom surface is equipped with the rectangle baffle, and the baffle slope sets up, is connected with fermentation tray bottom through an limit, and the fermentation tray is extended to the opposite side on this limit, and the lower surface of baffle is equipped with the wave line that sets up along the incline direction.
2. The preparation method of the bacterial cellulose film thickness capable of being controlled accurately according to claim 1, wherein the production device capable of being controlled accurately further comprises a leveling device, the leveling device comprises a control console, a film pre-pressing area and an arc-shaped cutter, the control console is used for controlling slicing thickness, the film pre-pressing area is provided with two groups of conveyor belts, each group of conveyor belts comprises an upper conveyor belt and a lower conveyor belt, the upper conveyor belts of the two groups of conveyor belts are identical in height, the lower conveyor belts are identical in height, the length of the arc-shaped cutter is smaller than the width of the conveyor belts, the arc-shaped cutter is horizontally arranged between the two groups of conveyor belts, and the length of the arc-shaped cutter is perpendicular to the length direction of the conveyor belts.
3. The bacterial cellulose membrane prepared by the preparation method capable of controlling the thickness of the bacterial cellulose membrane with precision according to claim 1.
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