CN208844108U - A kind of cell culture apparatus - Google Patents

Abstract

The utility model discloses a kind of cell culture apparatus, including box body, the tray interior is equipped with cellular compartments film, and the upper layer of the box body is divided into culture liquid layer by the cellular compartments film, and lower layer is divided into cell culture layer；The culture liquid layer is communicated with culture solution mouth；The cell culture layer is communicated with cell culture mouth；Inlet duct is equipped in the cell culture layer, the inlet duct includes equipped with the air pressure regulator above box body, and the air pressure regulator connects admission line, and the admission line is communicated in the air inlet layer of box body bottom, and the air inlet layer is equipped with air inlet.The utility model solves the problems, such as that cell culture apparatus can not high-volume serialization culture in the prior art.

Description

A kind of cell culture apparatus

Technical field

The utility model relates to cell culture auxiliary devices, equipment technical field, particularly belong to a kind of cell culture apparatus.

Background technique

Cell culture technology is also cell clone technology.For entire biotechnology, cell culture be all one must Indispensable process.A large amount of cell or its metabolite are obtained by cell culture.Because biological product is obtained from cell Come, it is possible to say that cell culture technology is most crucial, most basic technology in biotechnology.

Currently used cell large-scale cultivation method has the culture of regular growth culture bottle, spinner culture, bioreactor Culture and cell factory etc..But these culture vessels are only capable of doing batch cultur, are difficult to replace culture solution during culture and mention Cellular products are taken, culture, which finishes, to be both needed to disposably take out cell and culture solution, is not able to satisfy the requirement of the culture of serialization.And Above-mentioned culture vessel is generally only capable of doing the culture of small lot due to being not equipped with oxygen device for supplying, it is difficult to apply in industry In high-volume culture.

Utility model content

In consideration of it, solving cell culture apparatus in the prior art can not the utility model proposes a kind of cell culture apparatus The problem of high-volume serialization culture.

The utility model is achieved through the following technical solutions:

A kind of cell culture apparatus, including box body, the tray interior are equipped with cellular compartments film, and the cellular compartments film is by institute The upper layer for stating box body is divided into culture liquid layer, and lower layer is divided into cell culture layer；The culture liquid layer is communicated with culture solution mouth；Institute It states cell culture layer and is communicated with cell culture mouth；Inlet duct is equipped in the cell culture layer, the inlet duct includes setting There is the air pressure regulator above box body, the air pressure regulator connects admission line, and the admission line is communicated in box body bottom The air inlet layer of layer, the air inlet layer are equipped with air inlet.

One preferred embodiment of the utility model, wherein the culture solution mouth and the cell culture mouth have been respectively communicated with Culture liquid pipe and cell culture tube, the culture liquid pipe and the cell culture tube are respectively equipped with bottle cap；The cell culture fluid The bottle cap of pipe is equipped with air hole.

One preferred embodiment of the utility model, wherein the air inlet layer includes rubber section, if the rubber section is equipped with Do the air inlet.

One preferred embodiment of the utility model, wherein the diameter of the air inlet is 0.1mm~0.05mm.

One preferred embodiment of the utility model, wherein several spaced cells are equipped in the cell culture layer Culture plate, the tissue culture plate are obliquely or vertically arranged.

Working principle of the utility model is: adjusting air pressure regulator first, so that air inlet layer is kept certain air pressure, make air inlet Mouthful to external gas leakage, avoid anoxic inside cell culture layer, influence the growth and breeding of cell.By training needed for cell culture Nutrient solution is added to culture liquid layer from culture solution mouth.Culture solution is flowed into cell culture layer by cellular compartments film.Later by thin Born of the same parents cultivate mouth and the cell that need to be cultivated are added in cell culture layer.Cell growth and breeding in cell culture layer, and growth and Generated macromolecular product is trapped in cell culture layer by cellular compartments film during breeding.It is mentioned when needing to do part When taking and adding cell, is extracted and added from cell culture mouth, it is easy to operate to make the more efficient of cell culture.? In the incubation of cell, when needing replacing culture solution, culture solution is drawn out from culture solution mouth, or pours out, and from culture solution Mouth adds, easy to operate without separating cell.

Compared with prior art, the utility model has the beneficial effects that the cell culture layer independently opened is arranged in the utility model With culture liquid layer, separate cell and its product and cell culture fluid, it can be individually to cell and its product and cell culture Liquid processes, and enables the progress of cell culture serialization, saves the operating process of cell culture, increase production efficiency. The utility model is provided with the air inlet layer for cell oxygen supply in box body bottom, and when solving high-volume culture, cell is easy anoxic The problem of, apply the utility model in the high-volume culture of industry.

Detailed description of the invention

The utility model is described in further detail below with the drawings and specific embodiments:

Fig. 1 is the structural schematic diagram of the utility model.

Appended drawing reference: cellular compartments film 5, culture liquid layer 4, cell culture layer 9, air pressure regulator 1, admission line 11, into Gas-bearing formation 6, culture liquid pipe 7, cell culture tube 3, bottle cap 2, box body 10, tissue culture plate 12.

Specific embodiment

In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation Example, the present invention will be further described in detail.It should be appreciated that specific embodiment described herein is only used to explain The utility model is not used to limit the utility model.It should be noted that when element is referred to as " being fixed on " or " being set to " Another element, it directly on the other element or can may be simultaneously present centering elements.When an element is known as " being connected to " another element, it can be directly to another element or may be simultaneously present centering elements.Below The realization of the utility model is described in detail in conjunction with specific embodiments.

A kind of cell culture apparatus as shown in Figure 1, including box body 10 are equipped with cellular compartments film 5, cell point inside box body 10 The upper layer of box body 10 is divided into culture liquid layer 4 by diaphragm 5, and lower layer is divided into cell culture layer 9；If being equipped in cell culture layer 9 Do spaced tissue culture plate 12.When the cell of culture is adherent growth cell, it is more advantageous that tissue culture plate 12 is added Fast-growth and breeding in cell.Tissue culture plate 12 can be obliquely or vertically set in cell culture layer 9, be convenient for bottom The gas that layer is passed through passes through tissue culture plate 12.Culture liquid layer 4 is communicated with culture solution mouth；Cell culture layer 9 is communicated with cell training Support mouth；Inlet duct is equipped in cell culture layer 9, inlet duct includes the air pressure regulator 1 equipped with 10 top of box body, air pressure tune It saves valve 1 and connects admission line 11, admission line 11 is communicated in the air inlet layer 6 of 10 bottom of box body.Air inlet layer 6 includes rubber section, Rubber section is equipped with several air inlets.When gas inside air inlet layer 6 is pressurized to a certain extent, gas is from the air inlet in rubber section 6 bubblings in culture solution of mouth.When the air pressure deficiency in air inlet layer 6, due to the elastic reaction of rubber, air inlet can be closed It closes, avoids culture solution from blowing back into inside air inlet layer 6, culture solution is avoided to pollute air inlet layer 6.The diameter of air inlet is 0.1mm~0.05mm, the diameter are conducive to the culture solution supplemental oxygen slowly into cell culture layer, avoid air-flow excessive right The culture of cell causes adverse effect.Culture solution mouth and cell culture mouth have been respectively communicated with culture liquid pipe 7 and cell culture tube 3, Culture liquid pipe 7 and cell culture tube 3 are respectively equipped with bottle cap 2；The bottle cap 2 of cell culture liquid pipe 7 is equipped with air hole.By ventilative Hole makes the air of tray interior be easier to flow out, and is more advantageous to gas exchanges.

In use, adjusting air pressure regulator 1 first, air inlet layer 6 is made to keep certain air pressure, makes leaking to outside for air inlet Gas avoids 9 inside anoxic of cell culture layer, influences the growth and breeding of cell.By culture solution needed for cell culture from culture Liquid mouth is added to culture liquid layer 4.Culture solution is flowed into cell culture layer 9 by cellular compartments film 5.Later, pass through cell culture The cell that need to be cultivated is added in cell culture layer 9 by mouth.Cell growth and breeding in cell culture layer 9, and growth and breeding During generated macromolecular product be trapped in cell culture layer 9 by cellular compartments film 5.When needing to do extracting section When with addition cell, is extracted and added from cell culture mouth, it is easy to operate, and be capable of serialization and cultivate, Operating process is saved, the more efficient of cell culture is made.In the incubation of cell, when needing replacing culture solution, it will train Nutrient solution is drawn out from culture solution mouth, or is poured out, and is added from culture solution mouth, easy to operate without separating cell.

The cell culture layer 9 and culture liquid layer 4 that the utility model setting is independently opened, make cell and its product and cell culture Liquid separates, and can individually process to cell and its product and cell culture fluid, enable cell culture serialization into Row, saves the operating process of cell culture, increases production efficiency.It is cell that the utility model is provided in 10 bottom of box body The air inlet layer 6 of oxygen supply solves the problem of when in high volume cultivating, cell is easy anoxic, applies the utility model in work In the high-volume culture of industry.

Above-described is only preferred embodiments of the present invention, it is noted that for those skilled in the art For, under the premise of not departing from the utility model structure, several modifications and improvements can also be made, these also should be considered as this The protection scope of utility model, these all will not influence the effect and patent practicability of the utility model implementation.

Claims (5)

1. a kind of cell culture apparatus, including box body, it is characterised in that: the tray interior is equipped with cellular compartments film, the cell The upper layer of the box body is divided into culture liquid layer by separation membrane, and lower layer is divided into cell culture layer；The culture liquid layer is communicated with Culture solution mouth；The cell culture layer is communicated with cell culture mouth；Inlet duct, the air inlet are equipped in the cell culture layer Device includes equipped with the air pressure regulator above box body, and the air pressure regulator connects admission line, the admission line connection Set on the air inlet layer of box body bottom, the air inlet layer is equipped with air inlet.

2. a kind of cell culture apparatus as described in claim 1, it is characterised in that: the culture solution mouth and the cell culture mouth It has been respectively communicated with culture liquid pipe and cell culture tube, the culture liquid pipe and the cell culture tube is respectively equipped with bottle cap；It is described The bottle cap of cell culture liquid pipe is equipped with air hole.

3. a kind of cell culture apparatus as claimed in claim 1 or 2, it is characterised in that: the air inlet layer includes rubber section, described Rubber section is equipped with several air inlets.

4. a kind of cell culture apparatus as claimed in claim 3, it is characterised in that: the diameter of the air inlet be 0.1mm~ 0.05mm。

5. a kind of cell culture apparatus as described in claim 1,2 or 4, it is characterised in that: if being equipped in the cell culture layer Spaced tissue culture plate is done, the tissue culture plate is obliquely or vertically arranged.