CN201770705U - Outer circulation bioreactor for animal cell culture - Google Patents
Outer circulation bioreactor for animal cell culture Download PDFInfo
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- CN201770705U CN201770705U CN 201020278442 CN201020278442U CN201770705U CN 201770705 U CN201770705 U CN 201770705U CN 201020278442 CN201020278442 CN 201020278442 CN 201020278442 U CN201020278442 U CN 201020278442U CN 201770705 U CN201770705 U CN 201770705U
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- pipeline
- tank body
- jar
- canister
- external circulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
The utility model relates to an outer circulation bioreactor for animal cell culture. The bioreactor comprises a tank body, outer circulation pipelines, a heater, a peristaltic pump and a control system, wherein the tank body includes a large tank and a small tank, and the small tank is embedded inside the large tank and is in sleeve chuck fixation with the large tank respectively through a pipeline D, a pipeline A and a pipeline G; the outer circulation pipelines include a circulation pipeline outside the tank body, the pipeline A, a pipeline B, a pipeline E and the pipeline D, wherein the pipeline A and the pipeline B are arranged at the outer upper part of the tank body and connected with the circulation pipeline outside the tank body through T-joints, and the pipeline E and the pipeline D are arranged at the outer lower part of the tank body and connected with the circulation pipeline outside the tank body through T-joints; the circulation pipeline outside the tank body penetrates the peristaltic pump; and a blow-off pipe is connected to the lowermost part of the pipeline D. The utility model gives full play to the properties of large-scale animal cell culture technologies, has high functionality and economical efficiency as well as the remarkable characteristics of complete sterilization, simplicity of operation, low cell culture contamination rate, and high quality and yield, and can be widely used for microcarrier suspension animal cell culture and suspension animal cell culture.
Description
Technical field
The utility model relates to the device that the employing suspension process carries out animal cell culture, relates in particular to a kind of external circulation animal cell culture bioreactor.
Background technology
Bio-reactor refers to viable cell or enzyme to be that biological catalyst carries out the equipment that cell proliferation or biochemical reaction provide control environment, and it is the key equipment in the bioprocesses.Selected quality, yield (transformation efficiency) and the energy consumption to the biochemical industry product of the structure of bio-reactor, operating method and operational condition has substantial connection.
Continuous development along with biotechnology, extensive zooblast bioreactor culture technology just progressively replaces vial and leaves standstill cultivation and rotating and culturing technology, because it provides best living environment can in time cell and virus, thereby the output and the quality of the raising product of top efficiency, should this be a qualitative leap on the animal cell culture history, it also is the important breakthrough of medicine biological technique, it has not only simplified technology, the more important thing is that it can produce high-quality product, a variety of bio-reactors are arranged in the market, comprise home-made and import, respectively have superiority and defective, that have even have inevitable hard defects, thus limited the maximum performance of its advantage.
To compare analysis with regard to the design of aspects such as alr mode, supervisory system, sterilization method, ventilating mode, blowdown pattern to existing several animal cell culture bioreactors below, please see following table for details:
The utility model content
In view of the existing the problems referred to above of existing animal cell culture bioreactor, and for the quality of cell cultures, the influence that yield caused, the utility model is intended to disclose a kind of external circulation animal cell culture bioreactor, with the effect of effective assurance cell cultures, embody good and economic and functional.
Technical solution of the present utility model is achieved in that
A kind of external circulation animal cell culture bioreactor comprises tank body and external circulation line, well heater, peristaltic pump and Controlling System, it is characterized in that:
Described tank body comprises big jar and canister; Described big jar comprises big tank, water-bath interlayer and cover, and described cover is provided with ventage; Described canister comprises lower cover and middle tank body, and the described lower cover of going up is tightly connected with middle tank body respectively; By being arranged at lower cover and middle tank body junction on the described canister inside and, described canister being divided into three spaces of upper, middle and lower perpendicular to the web plate of stainless steel up and down of the axis of described canister;
Described canister is placed on described big jar inside, and fix by pipeline D, A, G and described big jar of cutting ferrule respectively, that is: the interior extreme lower position of lower cover of canister is fixed and be communicated to pipeline D one end, the bottom that its other end is arrogant jar is connected to outside big jar and by threeway and is connected with the I section and the pipeline E of jar external circulation line respectively, and the arrogant tank body of the other end of described pipeline E bottom is inserted in big jar; The extreme higher position that pipeline A one end is fixed in the upper cover of canister also is communicated in the canister since then, the top of the arrogant tank wall of its other end picks out outside the tank body, and be communicated with the II section and the pipeline B of jar external circulation line by threeway, pipeline B is equipped with diaphragm valve in this port, and the arrogant tank wall of the other end of described pipeline B middle and upper part is connected in big jar; The end of pipeline G is fixed in the upper cover of canister and passes the upper cover of canister and the middle tank body that last web plate is connected to described canister successively, and its other end is switched to outside the big tank;
Described external circulation line comprises pipeline E and the pipeline D that pipeline A that the outer top of a jar external circulation line, tank body is connected with the II section of described jar of external circulation line by threeway and pipeline B, the outer bottom of tank body are connected with the I section of described jar of external circulation line by threeway; Described jar of external circulation line passes described peristaltic pump; Described peristaltic pump adopts electrodeless variable-speed;
Also connect liquid respectively on the described jar of external circulation line and replenished pipeline, gas make-up pipeline, liquid collecting tube road;
Described pipeline D connects blow-off pipe at its lowest order.
Further, the monitoring probe that has also connected pH control and dissolved oxygen control on the II section of described jar of external circulation line, be placed with temperature controlled monitoring probe in the described big tank, above-mentioned monitoring probe is connected with housing by lead, and described housing comprises indicating meter, dissolved oxygen controller, pH controller, temperature regulator, datalogger.
Further, the arrogant tank body of described pipeline E bottom is inserted in big jar along big tank wall is tangential.
Further, the described lower cover of going up is tightly connected by external clip with middle tank body respectively.
Compared with prior art, the design of external circulation animal cell culture bioreactor described in the utility model has demonstrated fully the characteristics of animal cell large-scale culture technique, and layout of beam line is succinct; Have excellent function, reliability, accessibility, now specifically describe as follows:
(1) alr mode and sealing: utilize liquid circulation from bottom to top that nutrient solution and cell are stirred by peristaltic pump, fully airtight loop makes the PH, temperature, dissolved oxygen etc. of liquid reach full and uniform, and the shearing force of pair cell goes to zero; Solved the problem of the stirring of other form simultaneously to the seal request of tank body;
(2) ventilating mode: deep layer is directly ventilated, bubble fully not with cells contacting, thereby need not the pin bulb apparatus, can avoid the injury of bubble fully for cell;
(3) sterilization method: reasonable in design, layout of beam line is succinct, sterilizes in the moist heat sterilization cabinet, and is simple to operate, and the dead angle of not sterilizing also need not the maintenance management of pressing force container, has more security; Simultaneously shortened the production preparatory period greatly;
(4) blowdown pattern: can emit at any time by the discharge pipe that is in least significant end come off, apoptotic cells and broken microcarrier, reduced pollution rate;
Monitoring such as (5) supervisory system: PH, temperature and dissolved oxygen probe is installed on the outer circulation loop, and because of outer circulation makes liquid mixing full and uniform, so monitoring data is more objective, accurate and more representative.
To sum up, the utility model scientific structure design, layout of beam line is succinct, cell and nutrient solution are isolated cleverly, and best living environment is provided in time for cell and virus, evaded many hard defects and the defective that exists the existing installation from structure, the characteristics of animal cell large-scale culture technique have been given full play to, have sterilization thoroughly, simple to operate, the pollution of cell culture rate is low, quality is good, the distinguishing feature that yield is high has excellent function, economy, can be widely used in zooblast microcarrier suspension culture and zooblast suspension culture.
Description of drawings
Fig. 1 is the structural representation of the utility model embodiment;
Fig. 2 is the vertical view of Fig. 1.
Embodiment
Now in conjunction with the accompanying drawings, the utility model is further described specifically.
Described external circulation animal cell culture bioreactor, comprise tank body and external circulation line, well heater, peristaltic pump and Controlling System, wherein well heater, peristaltic pump and Controlling System adopt existing molding device, tank structure and external circulation line have then been done bigger innovation, as depicted in figs. 1 and 2:
Described tank body comprises big jar 1 and canister 2; Described big jar 1 comprises big tank 11, water-bath interlayer 12 and cover 13, one side lower part of described water-bath interlayer 12 is provided with hot water tube for entering can road F, the top of offside is provided with hot water and goes out a jar pipeline C, described hot water tube for entering can road F goes out jar pipeline C with hot water and is connected with the circulation line of well heater respectively, and described cover 13 is provided with ventage and connects breather line H, K; Described canister 2 comprises lower cover 21,23 and middle tank body 22, by external clip 24,25 the described lower cover of going up is tightly connected with middle tank body 22 respectively; Described canister 2 is inner to be gone up lower covers and middle tank body junction and perpendicular to the web plate of stainless steel up and down 26,27 of the axis of described canister, described canister 2 is divided into three spaces of upper, middle and lower by being arranged at;
Described canister 2 is placed on described big jar 1 inside, and fix by pipeline D, A, G and described big jar of 2 cutting ferrules respectively, that is: pipeline D one end fixes and is communicated to the extreme lower position in the lower cover 23 of canister by cutting ferrule, the bottom of the arrogant jar 1 of its other end is connected to outside big jar and by threeway and is connected with the I section and the pipeline E of jar external circulation line respectively, and the arrogant tank body of the other end of described pipeline E bottom is along in the big jar 1 of tangential access of big tank wall; Pipeline A one end by cutting ferrule fixing and and be communicated in the canister 2 from the extreme higher position of the upper cover 21 of canister, the top of the arrogant tank wall of its other end picks out outside the tank body, and be communicated with the II section and the pipeline B of jar external circulation line by threeway, pipeline B is equipped with diaphragm valve 6 in this port, and the arrogant tank wall of the other end of described pipeline B middle and upper part is connected in big jar 1; The end of pipeline G by cutting ferrule fixedly canister upper cover 21 and pass the upper cover 21 of canister and the middle tank body 22 that last web plate 26 is connected to described canister successively, its other end is switched to outside the tank body;
Described external circulation line comprises pipeline E and the pipeline D that pipeline A that the outer top of a jar external circulation line, tank body is connected with described jar of external circulation line by threeway and pipeline B, the outer bottom of tank body are connected with described jar of external circulation line by threeway; Described jar of external circulation line passes described peristaltic pump;
Enter the II section that the described jar of external circulation line that is connected by threeway with pipeline A and pipeline B before the peristaltic pump is referred to as jar external circulation line with aforementioned; The described jar of external circulation line that another section promptly comes out with pipeline E and pipeline D are connected by threeway of correspondence is referred to as the I section of jar external circulation line in described peristaltic pump; Automatic control probe, the additional pipeline 71 of liquid, gas make-up pipeline 72, liquid collecting tube road 73 have also been connected on the II section of described jar of external circulation line respectively, described automatic control probe comprises the monitoring probe 31 of pH control and the monitoring probe 32 of dissolved oxygen control, be placed with temperature controlled monitoring probe 33 in the big tank, above-mentioned monitoring probe is connected with housing by lead.
Described pipeline D connects blow-off pipe 5 at its lowest order.
The material of described tank body is the 316L stainless steel, and described water-bath interlayer is selected 304 stainless steels for use, the band thermal insulation layer.The tank body top is equipped with lamp mirror and visor, is used for inside reactor and observes.
When carrying out the tank body assembling, the last lower cover 21,23 of described canister connects with clip 24,25 respectively with middle tank body 22, by stainless steel web plate 26,27 middle tank body 22 being caught up with down two end sockets 21,23 keeps apart, lower cover 23 connects with pipeline D by the mode of cutting ferrule, upper cover connects with pipeline A, G by cutting ferrule, after built-in canister 2 fixes, cover big jar cover 13, whole tank body assembling finishes.
The connection of tank body pipeline comprises that pipeline C, F are connected to form big jar heating in water bath loop respectively with well heater; Pipeline A, B (connecting diaphragm valve 6) also insert liquid respectively and replenish pipeline, gas make-up pipeline, liquid collecting tube road and comprise pH control and the monitoring probe of dissolved oxygen control by the II section of stainless steel threeway connection jar external circulation line on the II section of described pipeline; Pipeline E, D connect the I section of jar external circulation line by the stainless steel threeway; Described jar of external circulation line passes described peristaltic pump; Pipeline H, K are connected on the ventage of big jar cover 13, and pipeline G is the passage that built-in canister 2 connects the tank body outsides.
Sterilising treatment:
After above-mentioned tank body assembling and pipe connection are finished, directly whole big jar is put into that to carry out sterilising treatment in the moist heat sterilization cabinet standby, so just do not need to do the checking of very complicated on-line cleaning and on-line cleaning, the more important thing is and can not stay the sterilization dead angle because of online sterilization, thereby greatly reduce pollution rate, also do not need the pressing force container to come use and management.About 1 day of above-mentioned sterilising treatment link required time, simple to operate, sterilization is thoroughly; And on average about 7 days of the online vapor sterilization processing links required time of existing installation, complicated operation stays the sterilization dead angle easily, pollutes, and must the maintenance management of pressing force container.
Stir control:
Realize the liquid circulation stirring by peristaltic pump.Microcarrier and cell are sent into by pipeline G in the middle tank body 22 of canister, and by web plate 26,27 liquid of microcarrier and cell and big jar are isolated; Under the effect of peristaltic pump, the liquid in the canister is mobile from bottom to top, and the liquid in the big jar rotates and produces eddy current, realizes that liquid fully stirs, and parameters such as fluid temperature, pH value, dissolved oxygen are in full accord, and recycle pump adopts electrodeless variable-speed, operates steadily.
Controlling System:
The reactor controlled function is realized by housing, comprises display screen, dissolved oxygen controller, PH controller, temperature regulator, datalogger.Wherein,
Temperature control:
Adopt the water-bath interlayer that reactor is carried out temperature control.The water-bath interlayer is equipped with water-bath heater, and water-bath heater has improved the susceptibility and the stability of temperature of reactor control.Reactor temperature control system adopts the pid control mode based on the tank body temperature, and temperature compensation function makes temperature control more stable, temperature control precision ± 0.3 ℃.
PH control:
Adopt the standalone module control mode, the pH controller is provided with separately.Use the PID master mode to realize control automatically, have Adaptive PID Control and adjust function, the pid parameter of automatically setting the best.PH control and carbonic acid gas breather valve, and acid, alkali peristaltic pump is associated, and by the switch of control carbonic acid gas, acid solution, alkali lye, realizes the automatic control of pH.When pH was lower than set(ting)value, controller started the alkali lye peristaltic pump, mended alkali and improved pH to set(ting)value; When pH was higher than set(ting)value, controller started the carbon dioxide magnetic valve, and the acid solution peristaltic pump, reduced pH to set(ting)value.Carbon dioxide flow can be regulated by spinner-type flowmeter, and acid solution, alkali lye peristaltic pump flow can be regulated by the pump speed-regulating valve, has further improved the sensitivity of pH control, pH control response deviation ± 0.01.
Dissolved oxygen control:
Adopt the standalone module control mode, the dissolved oxygen controller is provided with separately.Use the PID master mode to realize control automatically, have Adaptive PID Control and adjust function, the pid parameter of automatically setting the best.Dissolved oxygen control is related with the oxygen ventilation control valve, by the control valve switch, realizes the automatic control of dissolved oxygen.When dissolved oxygen is lower than set(ting)value, controller start-up control valve, logical oxygen improves dissolved oxygen to set(ting)value in reactor.Oxygen flow can be regulated by spinner-type flowmeter, has further improved the sensitivity of dissolved oxygen control, dissolved oxygen response variance 0.5%.
The process pipeline system:
Wherein, gas system comprises pressurized air gas piping, oxygen gas pipeline, carbon dioxide pipeline, gas pressure reducer, and pneumatic diaphragm valve is controlled automatically, be connected with a jar external circulation line by the gas make-up pipeline respectively, and under the effect of peristaltic pump, import in the tank body.
Liquid system comprises that liquid replenishes pipeline and liquid collecting tube road, is connected with the jar external circulation line respectively, and under the peristaltic pump effect feed liquid is added to enter in the tank body or in tank body and receive liquid.
The process implementing flow process
Cell cultures:
Connect all pipelines by aseptic requirement, inject about cell culture fluid 25L in big jar by peristaltic pump 4, water-bath is preheated to 25-28 ℃ then, and cell and microcarrier directly inject built-in canister by pipeline G, and total liquid is 30L-32L in the at this moment whole jar.Turn off pipeline B and E, low rate start peristaltic pump 4, allow cell fully be attached at (also can allow cell attach good back injects in the built-in canister) on the microcarrier up to cell outside jar at circulation some hrs in the built-in canister, allow temperature slowly be raised to 37 ℃ ± 0.3 ℃ simultaneously, pass through pipeline D behind the circulation some hrs outside the cell drain tank that does not attach, opening conduits B and E then, make the liquid in big jar also participate in the canister circulation, the process of liquid circulation also is to the well-beaten process of jar inner cell in the canister, slowly flowing fluid can not damage by pair cell, and liquid is mobile from bottom to top, does not only have the dead angle, and stirs very even.Data according to dissolved oxygen and the detection of pH probe, can turn off pipeline A, D earlier, circulate to big jar by peristaltic pump injection oxygen and sodium bicarbonate, gas and sodium bicarbonate be not directly with cells contacting like this, can avoid the too high pair cell of bubble and local PH to damage, after mixing up, opening conduits A, D circulate, thereby allow the interior physical and chemical index unanimity of big canister.
The cleaning of cell:
By pipeline E, D pot liquid is bled off, fill with the washing lotion circulation by peristaltic pump then, by pipeline E, D pot liquid is bled off again after the some time, look repeatedly repeated washing of particular case.
Virus culture:
Inject in big jar about virus-culturing fluid 25L by peristaltic pump, seed culture of viruses directly injects built-in canister by pipeline G, and total liquid is 30L-32L in the at this moment whole jar.Turn off pipeline B and E, the low rate start peristaltic pump, allow liquid circulation some hrs in built-in canister make fully cells infected of virus, allow temperature remain on 35 ℃ ± 0.3 ℃, behind the circulation some hrs by pipeline D outside the cell drain tank that comes off, opening conduits B and E then, make the liquid in big jar also participate in the canister circulation, data according to dissolved oxygen and the detection of PH probe, can turn off pipeline A earlier, D, circulate to big jar by peristaltic pump injection oxygen and sodium bicarbonate, gas and sodium bicarbonate directly with cells contacting, can not avoid the too high pair cell of bubble and local PH to damage, after mixing up like this, opening conduits A, D circulates, thereby allows the interior physical and chemical index unanimity of big canister.After cultivating some hrs, infected viral cell (disposable results) according to different goods results, or collected virus-culturing fluid (keep situation according to cell and can repeatedly repeat to gather in the crops virus-culturing fluid), so just finished a production cycle by pipeline E, D.
The utility model can provide the external circulation animal cell culture bioreactor of working volume 30-150L.
The above; it only is the preferable embodiment of the utility model; but protection domain of the present utility model is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the utility model discloses; be equal to replacement or change according to the technical solution of the utility model and utility model design thereof, all should be encompassed within the protection domain of the present utility model.
Claims (4)
1. an external circulation animal cell culture bioreactor comprises tank body and external circulation line, well heater, peristaltic pump and Controlling System, it is characterized in that:
Described tank body comprises big jar and canister; Described big jar comprises big tank, water-bath interlayer and cover, and described cover is provided with ventage; Described canister comprises lower cover and middle tank body, and the described lower cover of going up is tightly connected with middle tank body respectively; By being arranged at lower cover and middle tank body junction on the described canister inside and, described canister being divided into three spaces of upper, middle and lower perpendicular to the web plate of stainless steel up and down of the axis of described canister;
Described canister is placed on described big jar inside, and fix by pipeline D, A, G and described big jar of cutting ferrule respectively, that is: the interior extreme lower position of lower cover of canister is fixed and be communicated to pipeline D one end, the bottom that its other end is arrogant jar is connected to outside big jar and by threeway and is connected with the I section and the pipeline E of jar external circulation line respectively, and the arrogant tank body of the other end of described pipeline E bottom is inserted in big jar; The extreme higher position that pipeline A one end is fixed in the upper cover of canister also is communicated in the canister since then, the top of the arrogant tank wall of its other end picks out outside the tank body, and be communicated with the II section and the pipeline B of jar external circulation line by threeway, pipeline B is equipped with diaphragm valve in this port, and the arrogant tank wall of the other end of described pipeline B middle and upper part is connected in big jar; The end of pipeline G is fixed in the upper cover of canister and passes the upper cover of canister and the middle tank body that last web plate is connected to described canister successively, and its other end is switched to outside the big tank;
Described external circulation line comprises pipeline E and the pipeline D that pipeline A that the outer top of a jar external circulation line, tank body is connected with described jar of external circulation line by threeway and pipeline B, the outer bottom of tank body are connected with described jar of external circulation line by threeway; Described jar of external circulation line passes described peristaltic pump; Described peristaltic pump adopts electrodeless variable-speed;
Also connect liquid respectively on the described jar of external circulation line and replenished pipeline, gas make-up pipeline, liquid collecting tube road;
Described pipeline D connects blow-off pipe at its lowest order.
2. external circulation animal cell culture bioreactor according to claim 1 is characterized in that:
The monitoring probe that has also connected pH control and dissolved oxygen control on the II section of described jar of external circulation line, be placed with temperature controlled monitoring probe in the described big tank, above-mentioned monitoring probe is connected with housing by lead, and described housing comprises indicating meter, dissolved oxygen controller, pH controller, temperature regulator, datalogger.
3. external circulation animal cell culture bioreactor according to claim 1 and 2 is characterized in that:
The arrogant tank body of described pipeline E bottom is inserted in big jar along big tank wall is tangential.
4. external circulation animal cell culture bioreactor according to claim 3 is characterized in that:
The described lower cover of going up is tightly connected by external clip with middle tank body respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020278442 CN201770705U (en) | 2010-07-29 | 2010-07-29 | Outer circulation bioreactor for animal cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020278442 CN201770705U (en) | 2010-07-29 | 2010-07-29 | Outer circulation bioreactor for animal cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201770705U true CN201770705U (en) | 2011-03-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201020278442 Expired - Fee Related CN201770705U (en) | 2010-07-29 | 2010-07-29 | Outer circulation bioreactor for animal cell culture |
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| CN (1) | CN201770705U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484445A (en) * | 2019-08-30 | 2019-11-22 | 英诺维尔智能科技（苏州）有限公司 | A kind of fluid circulating device suitable for the production of scale biological products |
| CN113862147A (en) * | 2021-11-16 | 2021-12-31 | 陕西麦可罗生物科技有限公司 | Electromagnetic coupling embedded double-tank device and application thereof |
-
2010
- 2010-07-29 CN CN 201020278442 patent/CN201770705U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110484445A (en) * | 2019-08-30 | 2019-11-22 | 英诺维尔智能科技（苏州）有限公司 | A kind of fluid circulating device suitable for the production of scale biological products |
| CN113862147A (en) * | 2021-11-16 | 2021-12-31 | 陕西麦可罗生物科技有限公司 | Electromagnetic coupling embedded double-tank device and application thereof |
| CN113862147B (en) * | 2021-11-16 | 2024-02-27 | 陕西麦可罗生物科技有限公司 | Electromagnetic coupling embedded double-tank device and application thereof |
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Granted publication date: 20110323 Termination date: 20130729 |