CN110484445A - A kind of fluid circulating device suitable for the production of scale biological products - Google Patents
A kind of fluid circulating device suitable for the production of scale biological products Download PDFInfo
- Publication number
- CN110484445A CN110484445A CN201910811587.7A CN201910811587A CN110484445A CN 110484445 A CN110484445 A CN 110484445A CN 201910811587 A CN201910811587 A CN 201910811587A CN 110484445 A CN110484445 A CN 110484445A
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- Prior art keywords
- liquid circulation
- liquid
- circulation conduit
- production
- conduit
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
- C12M29/08—Air lift
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
Abstract
The present invention relates to biological products production technical fields, in particular a kind of fluid circulating device suitable for the production of scale biological products, including container body, the container body is provided with second liquid circulation conduit, the first liquid circulation conduit, the middle of second liquid circulation conduit is provided with peristaltic pump, the first liquid circulation conduit upper right side and the upper left side of second liquid circulation conduit offer liquid circulation outlet, the lower left side of the first liquid circulation conduit lower right side and second liquid circulation conduit offers liquid circulation suction inlet, gas inlet passages are uniformly provided on the downside of the container body, culture solution, which is uniformly provided with, on the upside of the container body enters pipeline, height independence can be kept by peristaltic pump and liquid line, it designs and produces into convenient for vessel with liquid circulation line primary Property consumption class product, further increase the safety of production process, reduce the risk of cross contamination between batch.
Description
Technical field
The present invention relates to biological products production technical field, specially a kind of stream suitable for the production of scale biological products
Body circulation device.
Background technique
Biologics, such as oncolytic virus (using cell as culture produce carrier), UCART, be now in high speed research and development with
Qualify Phase, it is main that manual mode is relied on to carry out sample preparation in development phase.The drug of present clinical test, soon
The use of scale of input in the future, the biologics of every batch of production should be able to meet making for tens of patients even patients up to a hundred
With, 50 to 200 liters of the liquid measure of every batch of pharmaceutical production, the volume of Ji Geng great.In the production process of large capacity, the life of most critical
Production. art requires to be to ensure to seek in technical process with culture as the cell of direct drug product or pharmaceutical production host cell
Dissolved gas etc. in feeding substance, culture solution is effectively contacted and is exchanged.Conventional antibodies pharmaceutical production fermentor or biology are anti-
Answer device although and may be implemented the liquid agitation and mixing in large-rolume container, but its rigid leaflet chip mechanical agitator institute for using
The mechanical shear stress of generation is also easy to produce the damage and death of cell.The novel biologics large-scale production based on cell, needs
One Large Copacity production reactor efficient, without mechanical damage damage force, safety stirs and evenly mixs system, realizes new bio
The large-scale production of drug, it is therefore desirable to it is a kind of suitable for scale biological products production fluid circulating device to the above problem
Make improvement.
Summary of the invention
The purpose of the present invention is to provide a kind of fluid circulating devices suitable for the production of scale biological products, to solve
The problems mentioned above in the background art.
To achieve the above object, the invention provides the following technical scheme:
A kind of fluid circulating device suitable for the production of scale biological products, including container body, the container body
Left side be provided with the first liquid circulation conduit, be provided with second liquid circulation conduit on the right side of the container casing, described
One liquid circulation conduit, second liquid circulation conduit middle be provided with peristaltic pump, the first liquid circulation conduit is right
Upside and the upper left side of second liquid circulation conduit offer liquid circulation outlet, the first liquid circulation conduit bottom right
The lower left side of side and second liquid circulation conduit offers liquid circulation suction inlet, and the downside of the container body uniformly opens up
There are gas inlet passages, is uniformly provided with culture solution on the upside of the container body and enters pipeline.
Preferably, 2 are respectively arranged in 90 ° of angles between the first liquid circulation conduit, second liquid circulation conduit
It is a.
Preferably, the liquid circulation outlet is located at the first liquid circulation conduit, second liquid circulation conduit
Upper end lower wall is uniformly provided with 3.
Preferably, the liquid circulation suction inlet is located at the first liquid circulation conduit, second liquid circulation conduit
Lower end upper side wall is uniformly provided with 3.
Preferably, the liquid circulation outlet is corresponding with liquid circulation suction inlet opens up, and the liquid circulation is arranged
Outlet is provided with downward fluid circulation canal with liquid circulation suction inlet.
Preferably, the gas inlet passages, culture solution enter 2 one group of pipeline and offer 4 groups, and the gas
Enter that pipeline is corresponding up and down to be opened up into channel and culture solution.
Preferably, the gas inlet passages, culture solution enter every group of pipeline respectively with the first liquid circulation conduit,
In 45 ° of angle settings between two liquid circulation conduits.
Preferably, the peristaltic pump uses BS300-1C specifications and models.
Preferably, including step used below:
S1, seed sample enter production containers main body, enter pipeline injection culture liquid by culture solution and reach full liquid
State makes entirety have circulation perfusion conditions, initially enters working cycles；
S2, peristaltic pump are started to work, and the liquid inside container body respectively enters first from into liquid circulation suction inlet
Liquid circulation conduit, second liquid circulation conduit, circulation loop lift peristaltic pump by liquid give rise to the first liquid circulation conduit,
The top of second liquid circulation conduit, liquid are discharged from liquid circulation outlet；
S3, liquid circulation outlet discharge, respectively enters the first liquid circulation conduit, the second liquid by liquid circulation suction inlet
Body circulation conduit, to Liquid extracting, makes liquid persistently flow downward under the action of peristaltic pump, and gas passes through gas inlet passages
It into container body, cultivates liquid and flows from the top to the bottom, gas moves from bottom to top, and the substance formed in production process is effectively mixed
It is even.
Compared with prior art, the beneficial effects of the present invention are:
In the present invention, by the whole liquid circulation for realizing device without mixing, avoid tradition machinery blender to cell and its
The impact and damage of allogene carrier, significantly optimization conventional leaf blender bring the non-uniform problem of Flow Field Distribution, significantly
It improves nutriment, the uniformity that dissolved gas is distributed inside container, realizes the more efficient benefit in culture production process, exempt from
Except the shaft and sealing of conventional leaf blender, full cavity and pipeline realize stringent sealing, and pollution risk, the pump housing is effectively reduced
(peristaltic pump) and liquid line can keep height independence, design and produce into convenient for vessel with liquid circulation line disposable
Class product is consumed, the safety of production process is further increased, reduces the risk of cross contamination between batch；
Detailed description of the invention
Fig. 1, which is that the present invention is whole, recycles basic structure front view；
Fig. 2 is well floor layout structure figure of the present invention；
Fig. 3 is cavity top plate layout of the present invention.
In figure: 1- container body, the first liquid circulation of 2- conduit, 3- second liquid circulation conduit, 4- peristaltic pump, 5- liquid
Circulation outlet, 6- liquid circulation suction inlet, 7- gas inlet passages, 8- culture solution enter pipeline.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments, is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-3 is please referred to, the present invention provides a kind of technical solution:
A kind of fluid circulating device suitable for the production of scale biological products, including container body 1, container body 1
Left side is provided with the first liquid circulation conduit 2, and the right side of container casing 1 is provided with second liquid circulation conduit 3, and the first liquid follows
Ring conduit 2, second liquid circulation conduit 3 middle be provided with peristaltic pump 4,2 upper right side of the first liquid circulation conduit and the
The upper left side of two liquid circulation conduits 3 offers liquid circulation outlet 5,2 lower right side of the first liquid circulation conduit and second
The lower left side of liquid circulation conduit 3 offers liquid circulation suction inlet 6, the downside of container body 1 be uniformly provided with gas into
Enter channel 7, the upside of container body 1 is uniformly provided with culture solution and enters pipeline 8.
2 are respectively arranged in 90 ° of angles between the first liquid circulation conduit 2, second liquid circulation conduit 3.
The liquid circulation outlet 5 is located at the upper end of the first liquid circulation conduit 2, second liquid circulation conduit 3
Lower wall is uniformly provided with 3.
The liquid circulation suction inlet 6 is located at the lower end of the first liquid circulation conduit 2, second liquid circulation conduit 3
Upper side wall is uniformly provided with 3.
The liquid circulation outlet 5 is corresponding with liquid circulation suction inlet 6 to be opened up, and the liquid circulation outlet 5
Liquid circulation suction inlet 6 is provided with downward fluid circulation canal.
The gas inlet passages 7, culture solution enter 82 one group of pipeline and offer 4 groups, and the gas enters
Channel 7 enters that about 8 pipeline is corresponding to be opened up with culture solution.
The gas inlet passages 7, culture solution enter 8 every groups of pipeline respectively with the first liquid circulation conduit 2, the second liquid
In 45 ° of angle settings between body circulation conduit 3.
The peristaltic pump 4 uses BS300-1C specifications and models.
Including step used below:
S1, seed sample enter production containers main body 1, enter the injection culture liquid of pipeline 8 by culture solution and reach full
Liquid status makes entirety have circulation perfusion conditions, initially enters working cycles；
S2, peristaltic pump 4 are started to work, and the liquid inside container body 1 respectively enters the from entrance liquid circulation suction inlet 6
One liquid circulation conduit 2, second liquid circulation conduit 3, circulation loop lift peristaltic pump 4 and liquid are given rise to the first liquid circulation
The top of conduit 2, second liquid circulation conduit 3, liquid are discharged from liquid circulation outlet 5；
S3, liquid circulation outlet 5 are discharged, and respectively enter the first liquid circulation conduit 2, the by liquid circulation suction inlet 6
Two liquid circulation conduits 3, to Liquid extracting, make liquid persistently flow downward under the action of peristaltic pump 4, gas by gas into
Enter channel 7 and enter container body 1, culture liquid flows from the top to the bottom, and gas moves from bottom to top, forms the object in production process
Matter effectively mixes.
Workflow of the present invention: in use, seed sample enters production containers main body 1, pipeline 8 is entered by culture solution
Injection culture liquid reaches full liquid status, and entirety is made to have circulation perfusion conditions, initially enters working cycles, and peristaltic pump 4 starts
It works, the liquid inside container body 1 respectively enters the first liquid circulation conduit 2, the second liquid from into liquid circulation suction inlet 6
Body circulation conduit 3, circulation loop lift peristaltic pump 4 and liquid are given rise to the first liquid circulation conduit 2, second liquid circulation conduit
3 top, liquid from liquid circulation outlet 5 be discharged, liquid circulation outlet 5 be discharged, by liquid circulation suction inlet 6 respectively into
Enter the first liquid circulation conduit 2, second liquid circulation conduit 3 under the action of peristaltic pump 4 to Liquid extracting, make liquid continue to
Lower flowing, gas enter container body 1 by gas inlet passages 7, and culture liquid flows from the top to the bottom, and gas is transported from bottom to top
Dynamic, the substance formed in production process effectively mixes.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (9)
1. a kind of fluid circulating device suitable for the production of scale biological products, including container body (1), it is characterised in that:
It is provided with the first liquid circulation conduit (2) on the left of the container body (1), is provided on the right side of the container casing (1)
Two liquid circulation conduits (3), the first liquid circulation conduit (2), second liquid circulation conduit (3) middle be provided with
Peristaltic pump (4), the first liquid circulation conduit (2) upper right side and the upper left side of second liquid circulation conduit (3) offer
Liquid circulation outlet (5), the lower left side of the first liquid circulation conduit (2) lower right side and second liquid circulation conduit (3)
It offers liquid circulation suction inlet (6), is uniformly provided with gas inlet passages (7) on the downside of the container body (1), it is described
It is uniformly provided with culture solution on the upside of container body (1) and enters pipeline (8).
2. a kind of fluid circulating device suitable for the production of scale biological products according to claim 1, feature exist
In: 2 are respectively arranged in 90 ° of angles between the first liquid circulation conduit (2), second liquid circulation conduit (3).
3. a kind of fluid circulating device suitable for the production of scale biological products according to claim 1, feature exist
In: the liquid circulation outlet (5) is located at the upper end of the first liquid circulation conduit (2), second liquid circulation conduit (3)
Lower wall is uniformly provided with 3.
4. a kind of fluid circulating device suitable for the production of scale biological products according to claim 1, feature exist
In: the liquid circulation suction inlet (6) is located at the lower end of the first liquid circulation conduit (2), second liquid circulation conduit (3)
Upper side wall is uniformly provided with 3.
5. a kind of fluid circulating device suitable for the production of scale biological products according to claim 1, feature exist
In: the liquid circulation outlet (5) is corresponding with liquid circulation suction inlet (6) to be opened up, and the liquid circulation outlet (5)
Liquid circulation suction inlet (6) is provided with downward fluid circulation canal.
6. a kind of fluid circulating device suitable for the production of scale biological products according to claim 1, feature exist
In: the gas inlet passages (7), culture solution enter (8) 2 one group of pipeline and offer 4 groups, and gas entrance is logical
Road (7) enters that pipeline (8) is corresponding up and down to be opened up with culture solution.
7. a kind of fluid circulating device suitable for the production of scale biological products according to claim 1, feature exist
In: the gas inlet passages (7), culture solution enter every group of pipeline (8) respectively with the first liquid circulation conduit (2), second
In 45 ° of angle settings between liquid circulation conduit (3).
8. a kind of fluid circulating device suitable for the production of scale biological products according to claim 1, feature exist
In: the peristaltic pump (4) uses BS300-1C specifications and models.
9. a kind of fluid circulating device suitable for the production of scale biological products according to claim 1, feature exist
In, including step used below:
S1, seed sample enter production containers main body (1), enter pipeline (8) injection culture liquid by culture solution and reach full
Liquid status makes entirety have circulation perfusion conditions, initially enters working cycles；
S2, peristaltic pump (4) are started to work, and the internal liquid of container body (1) is respectively enterd from into liquid circulation suction inlet (6)
First liquid circulation conduit (2), second liquid circulation conduit (3), circulation loop lift peristaltic pump (4) and liquid are given rise to first
The top of liquid circulation conduit (2), second liquid circulation conduit (3), liquid are discharged from liquid circulation outlet (5)；
S3, liquid circulation outlet (5) discharge, by liquid circulation suction inlet (6) respectively enter the first liquid circulation conduit (2),
Second liquid circulation conduit (3) to Liquid extracting, makes liquid persistently flow downward, gas passes through under the action of peristaltic pump (4)
Gas inlet passages (7) enter container body (1), and culture liquid flows from the top to the bottom, and gas moves from bottom to top, form production
Substance in the process effectively mixes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910811587.7A CN110484445A (en) | 2019-08-30 | 2019-08-30 | A kind of fluid circulating device suitable for the production of scale biological products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910811587.7A CN110484445A (en) | 2019-08-30 | 2019-08-30 | A kind of fluid circulating device suitable for the production of scale biological products |
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| CN110484445A true CN110484445A (en) | 2019-11-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910811587.7A Pending CN110484445A (en) | 2019-08-30 | 2019-08-30 | A kind of fluid circulating device suitable for the production of scale biological products |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1409546A (en) * | 1972-09-08 | 1975-10-08 | Boiko V I | Apparatus for cultivating microorganisms |
| GB1496813A (en) * | 1974-03-18 | 1978-01-05 | Muller H | Apparatus for the combined cooling and aeration of bioreactors for carrying out micro-biological conversions |
| CN201770705U (en) * | 2010-07-29 | 2011-03-23 | 罗火生 | Outer circulation bioreactor for animal cell culture |
| CN108404568A (en) * | 2018-03-31 | 2018-08-17 | 南安建金工业设计有限公司 | A kind of spray column of liquid active carbon function |
| CN109477054A (en) * | 2016-07-22 | 2019-03-15 | 日产化学株式会社 | The manufacturing method of liquid culture medium composition and manufacturing device for the manufacturing method |
-
2019
- 2019-08-30 CN CN201910811587.7A patent/CN110484445A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1409546A (en) * | 1972-09-08 | 1975-10-08 | Boiko V I | Apparatus for cultivating microorganisms |
| GB1496813A (en) * | 1974-03-18 | 1978-01-05 | Muller H | Apparatus for the combined cooling and aeration of bioreactors for carrying out micro-biological conversions |
| CN201770705U (en) * | 2010-07-29 | 2011-03-23 | 罗火生 | Outer circulation bioreactor for animal cell culture |
| CN109477054A (en) * | 2016-07-22 | 2019-03-15 | 日产化学株式会社 | The manufacturing method of liquid culture medium composition and manufacturing device for the manufacturing method |
| CN108404568A (en) * | 2018-03-31 | 2018-08-17 | 南安建金工业设计有限公司 | A kind of spray column of liquid active carbon function |
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Application publication date: 20191122 |