CN209201779U - Plant callus subculture increment culture apparatus - Google Patents
Plant callus subculture increment culture apparatus Download PDFInfo
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- CN209201779U CN209201779U CN201822131500.5U CN201822131500U CN209201779U CN 209201779 U CN209201779 U CN 209201779U CN 201822131500 U CN201822131500 U CN 201822131500U CN 209201779 U CN209201779 U CN 209201779U
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Abstract
The utility model provides a kind of plant callus subculture increment culture apparatus, comprising: double screw socket culture tubes, upper screw socket are equipped with exhaust decompression mouth and culture medium supply port, and lower screw socket is equipped with culture medium outlet；More mouthfuls are stirred container, and there is culture medium to return liquid mouth, air-in and culture medium delivery outlet, and the culture medium returns in liquid mouth inserted with returning liquid pipe road, it is described return liquid pipe road pile warp and return liquid peristaltic pump be connected to the culture medium outlet；The air-in is inserted with air pipeline, and the outer end of the air pipeline connects positive pressure filtrated air；Inserted with liquid-transport pipe-line in the culture medium delivery outlet, the liquid-transport pipe-line pile warp infusion peristaltic pump is connected to the culture medium supply port；Agitating device, for the medium liquid that more mouthfuls are stirred in container to be sufficiently mixed with filtrated air.The utility model has the advantages that high no shearing force, dissolved oxygen level, good mixing property and pollution rate are low.
Description
Technical field
The utility model relates to a kind of plant callus subculture increment culture apparatus.Suitable for being made using medium liquid
For nutrition supply source, the biomass of plant callus is enable to rise in value.
Background technique
In recent years, the deterioration due to ecological environment and the over-exploitation to wild plant, Wild Medicinal Plants Resources
Available quantity is reduced year by year.Although artificial cultivation can make up the deficiency of some Wild Medicinal amounts, because of land resource, agriculture
Medicine residual, seasonal effect and cultivation medicinal material active component content such as have differences at the factors, cause artificial cultivation that cannot be fully solved
The contradiction of medicine resource scarcity and demand cumulative year after year.
Currently, 60 various plants drugs, such as Japanese yew can be produced under artificial condition using plant cell culture technology
Alcohol, ginsenoside, shikonin etc..Vegetable culture technique includes the controllable of working condition, not climate, season, soil and region
Etc. the influence of a variety of conditions；Nonstaining property, there is no people's pesticides and heavy metal pollution problem introduced in cultivation；Because of people
Work conditional stability, so plant cell product quality has stability and consistency；The life of parsing target metabolic product can be passed through
Object route of synthesis targetedly improves the conjunction of target metabolic product by technique for gene engineering combination plant cell inductive condition
Cheng Liang；The production cycle of plant cell culture technology is shorter than traditional artificial cultivation period, can successional industrialized production etc. it is excellent
Point.
The culture apparatus that plant cell is mass produced at present is often carried out by the way of the culture that suspends,
It is general that stirring-type and non-stirred formula fermentation jar is selected to realize, it often has the following problems and feature:
1) sensitive to mechanical shear stress, plant cell is big, and deadlock is crisp to lack flexibility, while having vacuole so to mechanical detection
Power is sensitive.Interaction and solid and solid of the mechanical shear stress from the interaction of solid and liquid, gas and liquid
Interaction.Shearing force often leads to the division of plant cell aggregation, and the division of aggregation can change between plant cell
Contact and information transmitting.Intercellular information transmitting is blocked the growth that will affect cell.
2) aggregation occurs for plant cell forms maxicell group to be easy precipitating, and causing inside cell mass cannot adequately and nutrition
Substance is contacted.
3) dissolved oxygen level has large effect to the formation of Secondary Metabolism of Plant, and cell mass solid matter is mixed with culture solution
It will affect the solubility of air in a liquid.
4) it is stirred in culture plant cell device, is easy to produce mechanical shear stress, while hyperventilation is easy to lead
It causes plant cell excretion albumen to generate a large amount of drum, and then influences the growth of plant cell.Stirred-tank fermenter is stirred
Cheng Zhong, easily formation mechanical shear stress, are easy to produce detrimental effect to plant cell, influence the growth and metabolism of plant cell.It is non-
Stirred-tank fermenter can avoid the long-living Strong shear power of stirred-tank fermenter, while ventilation efficiency is better than stirred-tank fermenter, but
Be long time cultivation carry out culture solution replacement operation it is cumbersome, it is easy to pollute.It is updated squamous subculture simultaneously, operating process is numerous
It is trivial, it is easy to pollute.
As it can be seen that existing culture apparatus is there are shearing force height, dissolved oxygen level is low, mixed performance is low and what pollution rate was high lacks
Point.So developing a kind of novel plant cell culture apparatus, it can effectively solve traditional plant cell culture apparatus defect, be
One trend of current culture plant cell device development.
Utility model content
The main purpose of the utility model is to provide a kind of plant callus subculture increment culture apparatus, trains liquid
Nutrient solution periodically independently can be replaced or supplement, while the middle air supply modes of liquid medium are independent, prevent
Pollution problem.
The another object of the utility model is to provide a kind of plant callus subculture increment culture apparatus, improve with
Liquid medium supplies the mode of callus, avoids the damage because of stirring bring mechanical shear stress to callus cell
Wound.
In order to achieve the above purposes, the technical solution adopted by the utility model is:
A kind of plant callus subculture increment culture apparatus characterized by comprising
Double screw socket culture tubes, have upper screw socket and lower screw socket, and the upper plug screw of upper screw socket has an exhaust decompression mouth and one
The lower plug screw of a culture medium supply port, lower screw socket is equipped with culture medium outlet；
More mouthfuls are stirred container, and there is culture medium to return liquid mouth, air-in and culture medium delivery outlet, the culture medium
Return in liquid mouth that liquid level is below to return liquid pipe road inserted with submerging, the partial-length pile warp for returning liquid pipe road is returned liquid peristaltic pump and connected again
It is connected to the culture medium outlet；The bottle stopper of the air-in is equipped with exhaust outlet and air intake, in the air intake
It is inserted with air pipeline, the outer end of the air pipeline connects positive pressure filtrated air, and the inner end of the air pipeline is inserted into more mouthfuls
It is stirred the liquid level of container or less；It is described defeated inserted with submerging liquid level liquid-transport pipe-line below in the culture medium delivery outlet
The partial-length pile warp infusion peristaltic pump in liquid pipe road is connected to the culture medium supply port；
Agitating device, for the medium liquid that more mouthfuls are stirred in container to be sufficiently mixed with filtrated air.
The plant callus subculture increment culture apparatus, in which: in the liquid-transport pipe-line and return in liquid pipe road
Hydrophobic cavity filter membrane is equipped with close to one end of double screw socket culture tubes.
The plant callus subculture increment culture apparatus, in which: the exhaust decompression mouth and the exhaust outlet point
It is not connected with hydrophylic filter membranes or absorbent cotton.
The plant callus subculture increment culture apparatus, in which: the outer end of the air pipeline connects air pump,
Hydrophobic air filter membrane is equipped with inside the air pipeline.
The plant callus subculture increment culture apparatus, in which: sintered quartz is equipped in double screw socket culture tubes
Plate, sintered quartz plate is for plant callus placement.
The plant callus subculture increment culture apparatus, in which: described more mouthfuls are stirred container and are additionally provided with pH
Detection mouth is inserted with online pH meter in the bottle stopper of the pH detection mouth, and the test side of the online pH meter is inserted into more mouthfuls of stirrings
Below the liquid level of mixing vessel.
The plant callus subculture increment culture apparatus, in which: in addition, double screw socket culture tubes and thereon spiral shell
Plug, lower plug screw, described more mouthfuls be stirred container and its bottle stopper, the liquid-transport pipe-line, described return liquid pipe road and the air
Pipeline is all made of to be resistant to autoclaved material.
The plant callus subculture increment culture apparatus, in which: described more mouthfuls are stirred container and are placed in magnetic
Above power blender, and a magnetic stir bar is placed in the inside bottom side for being stirred at described more mouthfuls container, by the magnetic force
Blender and the magnetic stir bar constitute the agitating device.
The plant callus subculture increment culture apparatus, in which: the stirrer is to be resistant to high pressure sterilization
Material be made.
The plant callus subculture increment culture apparatus, in which: there are multiple double screw sockets being serially connected
Culture tube, the culture medium outlet positioned at double screw socket culture tubes of upstream are connected with the culture medium supply port for being located at downstream, are located at
The culture medium supply port of double screw socket culture tubes of head end is connected with the liquid-transport pipe-line, positioned at the training of double screw socket culture tubes of tail end
Base outlet is supported to be connected with the liquid pipe road that returns.
Compared with prior art, the utility model by adopting the above technical scheme with no shearing force, dissolved oxygen level it is high,
Good mixing property and the low advantage of pollution rate.
Detailed description of the invention
Fig. 1 is the overall structure diagram of plant callus subculture increment culture apparatus provided by the utility model.
Description of symbols: double screw socket culture tubes 1；Iron stand 2；Upper plug screw 3；Exhaust decompression mouth 4；Culture medium supply port 5；
Lower plug screw 6；Culture medium outlet 7；Sintered quartz plate 8；Plant callus 9；More mouthfuls are stirred container 10；Culture medium returns liquid
Mouth 11；Air-in 12；Culture medium delivery outlet 13；Return liquid pipe road 14；Return liquid peristaltic pump 15；Exhaust outlet 16；Air intake 17；
Air pipeline 18；Air pump 19；Liquid-transport pipe-line 20；Infusion peristaltic pump 21；PH detection mouth 22；Online pH meter 23；Magnetic stirring apparatus
24；Magnetic stir bar 25；Hydrophobic cavity filter membrane 26.
Specific embodiment
Hereinafter reference will be made to the drawings is described in detail by way of example and not limitation some specific embodiments of the present invention.
Identical appended drawing reference denotes same or similar part or part in attached drawing.It should be appreciated by those skilled in the art that these
Attached drawing is not necessarily to be depicted true to scale.
As shown in Figure 1, the utility model provides a kind of plant callus subculture increment culture apparatus, comprising:
Double screw socket culture tubes 1, are fixed on iron stand 2, have upper screw socket and lower screw socket, and the upper plug screw 3 of upper screw socket has
One exhaust decompression mouth 4 and a culture medium supply port 5, the exhaust decompression mouth 4 can connect 0.22 μm of hydrophylic filter membranes or de-
Rouge cotton (is unillustrated), and extraneous bacterium colony is avoided to enter, and the lower plug screw 6 of lower screw socket is equipped with culture medium outlet 7；In double screw socket cultures
Sintered quartz plate 8 is equipped in pipe 1, sintered quartz plate 8 is for the placement of plant callus 9；The purposes of the sintered quartz plate 8 is
The loss for preventing plant callus can also prevent plant callus from entering pipeline and generate blocking and pollution problem；
More mouthfuls are stirred container 10, and there is culture medium to return liquid mouth 11, air-in 12 and culture medium delivery outlet 13, institute
It states culture medium and returns in liquid mouth 11 that liquid level is below to return liquid pipe road 14, the partial-length pile warp for returning liquid pipe road 14 inserted with submerging
It returns liquid peristaltic pump 15 and is connected to the culture medium outlet 7, pressed, made in pipe in pipe outer periodic by returning liquid peristaltic pump 15
Liquid returns liquid mouth 11 to culture medium and flows, and since peristaltic pump does not contact liquid in pipe, will not generate pollution problem；The air is defeated
The bottle stopper of entrance 12 is equipped with exhaust outlet 16 and air intake 17, and the exhaust outlet 16 can connect 0.22 μm of hydrophylic filter membranes or degreasing
Cotton avoids extraneous bacterium colony from entering, and is inserted with air pipeline 18 in the air intake 17, (inside is equipped with the air pipeline 18
0.22 μm of hydrophobic air filter membrane) outer end connect air pump 19, it is mixed that the inner end of the air pipeline 18 is inserted into more mouthfuls of stirrings
Below the liquid level for closing container 10；It is described defeated inserted with submerging liquid level liquid-transport pipe-line 20 below in the culture medium delivery outlet 13
The partial-length pile warp infusion peristaltic pump 21 in liquid pipe road 20 is connected to the culture medium supply port 5, the infusion peristaltic pump 21
Liquid in pipe can be driven to flow to culture medium supply port 5, and avoid pollution problem；Described more mouthfuls are stirred container 10 and also set
There is pH detection mouth 22, online pH meter 23 is inserted in the bottle stopper of the pH detection mouth 22, the test side of the online pH meter 23 is inserted
Below the liquid level for entering to more mouthfuls to be stirred container 10；
Agitating device, for mixing well with air medium liquid；In the present embodiment, in order to avoid dirt as far as possible
Dye is stirred container 10 for described more mouthfuls and is placed in 24 top of magnetic stirring apparatus, and is stirred container 10 at described more mouthfuls
Inside bottom side place a magnetic stir bar 25, the magnetic stirring apparatus 24 is stirred the outside of container 10 at described more mouthfuls
Contactless driving is carried out to the magnetic stir bar 25, medium liquid is helped to be sufficiently mixed with filtrated air；
In order to further avoid pollution problem occur, in the liquid-transport pipe-line 20 and return in liquid pipe road 14 equipped with 0.22 μm
Hydrophobic cavity filter membrane 26,0.22 μm of hydrophobic cavity filter membrane 26 are preferably disposed on the liquid-transport pipe-line 20 and return liquid pipe road 14
Close to one end of double screw socket culture tubes 1.
In addition, double screw socket culture tubes 1 and thereon plug screw 3, lower plug screw 6, it is described more mouthfuls be stirred container 10 and its
Bottle stopper, the liquid-transport pipe-line 20, described returns liquid pipe road 14 and the air pipeline 18 is all with energy at the magnetic stir bar 25
It is enough resistant to autoclaved material to be made, in order to sterilization.
Moreover, the settable multiple double screw socket culture tubes 1 of the utility model and connecting, to carry out pilot scale culture.
When the utility model uses, double screw socket culture tubes 1 are fixed on iron stand 2, and plant callus is sterile
Under the conditions of be put into double screw socket culture tubes 1, the volume of plant callus is no more than the 1/5 of double 1 volumes of screw socket culture tube, institute
It states more mouthfuls of mixing stirring devices and is aseptically packed into suitable medium liquid, final volume is mixed to be no more than more mouthfuls of stirrings
Close device volume 3/4 is advisable；Joint adjusts infusion peristaltic pump 21 and returns the revolving speed of liquid peristaltic pump 15, and medium liquid is perfused
Extremely double screw socket culture tubes 1, and the volume of medium liquid in double screw socket culture tubes 1 is made to be no more than pair volume of screw socket culture tubes 1
4/5；Restart air pump 19, so that filtrated air is entered more mouthfuls and be stirred in container, and adjusts turning for magnetic stirring apparatus 24
Speed enables the more mouthfuls of magnetic stir bars 25 for being stirred 10 bottom of container sufficiently to mix medium liquid with filtrated air
It closes；By circulating for medium liquid, the oxygen in nutrient liquid and filtrated air is constantly conveyed with carbon dioxide
To the plant callus in double screw socket culture tubes 1, make its growth.
Since the flowing velocity of medium liquid is slow, generated mechanical shear stress is small, breaks to callus cell
Bad effect is small.Medium liquid is mixed with air to be stirred in container 10 in Duo Kou and be carried out, independent with double screw socket culture tubes 1,
Detrimental effect of the mechanical shear stress that avoidable air and liquid are mixed to form to plant callus.When plant callus is long
Between cultivate, it is only necessary to be stirred in container 10 in medium liquid certain ingredients and pH value (by online pH according to more mouthfuls of detection
23 progress real-time measurements of meter) height judged that then exchange for or Additional nutrient solution, this operation is mixed just for more mouthfuls of stirrings
10 part of container is closed, does not need frequently to operate double screw socket culture tubes 1, pollution caused by operation can be reduced, change liquid
Simply there is operability.When the plant callus growth in double screw socket culture tubes 1 reaches a certain amount of, it is only necessary to exchange for
Plant callus taking-up in pipe can be carried out subsequent processing operation, if desired continue culture and plant by double screw socket culture tubes 1
The new twoport spiral culture tube equipped with plant callus need to be only connected in system by object callus.
In short, the utility model has the advantages that high no shearing force, dissolved oxygen level, good mixing property and pollution rate are low.
It is described above to be merely exemplary for the utility model, and not restrictive, those of ordinary skill in the art
Understand, without departing from the spirit and scope defined by the claims, many modifications, variation or equivalent can be made, but
It falls within the protection scope of the utility model.
Claims (10)
- The culture apparatus 1. a kind of plant callus subculture rises in value characterized by comprisingDouble screw socket culture tubes, have upper screw socket and lower screw socket, and the upper plug screw of upper screw socket has an exhaust decompression mouth and a training Base supply port is supported, the lower plug screw of lower screw socket is equipped with culture medium outlet；More mouthfuls are stirred container, there is culture medium to return liquid mouth, air-in and culture medium delivery outlet, the culture medium returns liquid Liquid level is below to return liquid pipe road inserted with submerging in mouthful, and the partial-length pile warp for returning liquid pipe road is returned liquid peristaltic pump and is connected to The culture medium outlet；The bottle stopper of the air-in is equipped with exhaust outlet and air intake, is inserted in the air intake The outer end of air pipeline, the air pipeline connects positive pressure filtrated air, and the inner end of the air pipeline is inserted into more mouthfuls of stirrings Below the liquid level of mixing vessel；Inserted with submerging liquid level liquid-transport pipe-line below, the perfusion tube in the culture medium delivery outlet The partial-length pile warp infusion peristaltic pump in road is connected to the culture medium supply port；Agitating device, for the medium liquid that more mouthfuls are stirred in container to be sufficiently mixed with filtrated air.
- The culture apparatus 2. plant callus subculture according to claim 1 rises in value, it is characterised in that: in the perfusion tube Road and the one end for returning close double screw socket culture tubes in liquid pipe road are equipped with hydrophobic cavity filter membrane.
- The culture apparatus 3. plant callus subculture according to claim 1 rises in value, it is characterised in that: the exhaust decompression Mouth is connected separately with hydrophylic filter membranes or absorbent cotton with the exhaust outlet.
- The culture apparatus 4. plant callus subculture according to claim 1 rises in value, it is characterised in that: the air pipeline Outer end connect air pump, be equipped with hydrophobic air filter membrane inside the air pipeline.
- The culture apparatus 5. plant callus subculture according to claim 1 rises in value, it is characterised in that: in double screw socket cultures Sintered quartz plate is equipped in pipe, sintered quartz plate is for plant callus placement.
- The culture apparatus 6. plant callus subculture according to claim 1 rises in value, it is characterised in that: the more mouthfuls of stirrings Mixing vessel is additionally provided with pH detection mouth, and online pH meter, the detection of the online pH meter are inserted in the bottle stopper of the pH detection mouth End is inserted into more mouthfuls and is stirred the liquid level of container or less.
- The culture apparatus 7. plant callus subculture according to claim 1 rises in value, it is characterised in that: in addition, described double Screw socket culture tube and thereon plug screw, lower plug screw, described more mouthfuls be stirred container and its bottle stopper, the liquid-transport pipe-line, described return Liquid pipe road and the air pipeline are all made of to be resistant to autoclaved material.
- The culture apparatus 8. plant callus subculture according to claim 1 rises in value, it is characterised in that: the more mouthfuls of stirrings Mixing vessel is placed in above magnetic stirring apparatus, and the inside bottom side for being stirred at described more mouthfuls container is placed a magnetic force and stirred Son is mixed, the agitating device is constituted by the magnetic stirring apparatus and the magnetic stir bar.
- The culture apparatus 9. plant callus subculture according to claim 8 rises in value, it is characterised in that: the stirrer with Autoclaved material is resistant to be made.
- The culture apparatus 10. plant callus subculture according to claim 1 rises in value, it is characterised in that: there are multiple phases Mutual concatenated double screw socket culture tubes, the culture medium outlet positioned at double screw socket culture tubes of upstream and the culture positioned at downstream Base supply port is connected, and the culture medium supply port positioned at double screw socket culture tubes of head end is connected with the liquid-transport pipe-line, is located at tail end The culture medium outlets of double screw socket culture tubes be connected with the liquid pipe road that returns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822131500.5U CN209201779U (en) | 2018-12-18 | 2018-12-18 | Plant callus subculture increment culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822131500.5U CN209201779U (en) | 2018-12-18 | 2018-12-18 | Plant callus subculture increment culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209201779U true CN209201779U (en) | 2019-08-06 |
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ID=67465131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822131500.5U Expired - Fee Related CN209201779U (en) | 2018-12-18 | 2018-12-18 | Plant callus subculture increment culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209201779U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753576A (en) * | 2020-12-31 | 2021-05-07 | 武汉叶动力生物科技股份有限公司 | Plant tissue culture method and plant tissue culture container thereof |
| CN113331054A (en) * | 2021-05-31 | 2021-09-03 | 广西壮族自治区农业科学院 | Plant tissue culture method by adding culture medium under bacteria environment condition |
-
2018
- 2018-12-18 CN CN201822131500.5U patent/CN209201779U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753576A (en) * | 2020-12-31 | 2021-05-07 | 武汉叶动力生物科技股份有限公司 | Plant tissue culture method and plant tissue culture container thereof |
| CN113331054A (en) * | 2021-05-31 | 2021-09-03 | 广西壮族自治区农业科学院 | Plant tissue culture method by adding culture medium under bacteria environment condition |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190806 Termination date: 20191218 |