CN206396227U - A kind of hyaluronic acid production fermentation tank - Google Patents

Abstract

The utility model provides a kind of hyaluronic acid production fermentation tank, including tank body, it is respectively arranged on charge door and discharging opening at the top and bottom of tank body, agitating shaft in tank body and the agitating paddle located at agitating shaft bottom, pH meter and dissolved oxygen sensor are provided with tank body, agitating shaft and agitating paddle are cavity body, the cavity of agitating shaft is connected with the cavity of agitating paddle, agitating paddle is provided with the through hole communicated with agitating paddle cavity, the top of agitating shaft is connected by pipeline with soda acid liquid storage tank, pipeline is provided with liquid feeding pump, tank body inner bottom part is provided with oxygen air-spreading disk, oxygen air-spreading disk is connected by air inlet pipe with the apparatus of oxygen supply outside tank body, the stomata arranged on the upside of oxygen air-spreading disk provided with radial radiation.The fermentation tank can accurately, uniformly, automatically regulate and control the pH and dissolved oxygen content of zymotic fluid during production hyaluronic acid, improve the yield of hyaluronic acid.

Description

A kind of hyaluronic acid production fermentation tank

Technical field

The utility model is related to microbial fermentation apparatus field, more particularly, to a kind of hyaluronic acid production fermentation tank.

Background technology

Hyaluronic acid is widely used in many fields such as cosmetics, beauty industry, health food, medicine.Produce hyalomitome The common method of acid has animal tissue's extraction method, microbe fermentation method and chemical synthesis, wherein animal tissue's extraction method and change Learn synthetic method cost high, yield poorly, limited by raw material resources, it is impossible to meet the market demand, and Production by Microorganism Fermentation is transparent Matter acid has the advantages that not limited by raw material resources, cost is low, yield is high, the main method as hyaluronic acid production.

During Production by Microorganism Fermentation hyaluronic acid, dissolved oxygen content and pH value are to influence two passes of fermentation efficiency Key factor.When dissolved oxygen is less than critical dissolved oxygen 30% in zymotic fluid, anaerobic respiration causes acetic acid in zymotic fluid, ethanol content to increase, Suppress cell growth and product expression, influence hyaluronic acid volume of production.And cell thalline is produced in growth course under acid makes pH rapid Drop, change thalline living environment, make cellular biomass rapid development decline, in due course between adjust pH, make its be beneficial to thalline Breeding, so that it may extend its exponential phase, so as to improve the yield of hyaluronic acid.

Production by Microorganism Fermentation hyaluronic acid is often carried out in fermentation tank, therefore how fermentation tank preferably controls fermentation During zymotic fluid dissolved oxygen content and pH value be determine hyaluronic acid yield direct factor.

The content of the invention

Problem to be solved in the utility model is to provide a kind of hyaluronic acid production fermentation tank, can be accurately and uniform The dissolved oxygen content and pH value of fermentation cylinder for fermentation liquid in fermentation process are controlled, the yield of hyaluronic acid is improved.

In order to solve the above technical problems, the technical solution adopted in the utility model is：A kind of hyaluronic acid production fermentation Tank, including tank body, the charge door that is respectively arranged at the top and bottom of tank body and discharging opening, the agitating shaft in tank body and located at stirring Mix and pH meter and dissolved oxygen sensor are provided with the agitating paddle of axle bottom, tank body, agitating shaft and agitating paddle are cavity body, agitating shaft Cavity is connected with the cavity of agitating paddle, and agitating paddle is provided with the through hole communicated with agitating paddle cavity, and the top of agitating shaft passes through pipe Road is connected with soda acid liquid storage tank, and pipeline is provided with liquid feeding pump, and tank body inner bottom part is provided with oxygen air-spreading disk, and oxygen air-spreading disk is by entering Tracheae is connected with the apparatus of oxygen supply outside tank body, the stomata arranged on the upside of oxygen air-spreading disk provided with radial radiation.

In technical scheme, it is preferred that pipeline is provided with the first magnetic valve, air inlet pipe is provided with the second magnetic valve.

In technical scheme, it is preferred that be additionally provided with controller, controller respectively with pH meter, dissolved oxygen sensor, the first magnetic valve With the electrical connection of the second magnetic valve.

In technical scheme, it is preferred that oxygen air-spreading disk is below agitating paddle.

The utility model has the advantages and positive effects of：It can more accurate, uniform, automatically regulate and control production hyalomitome The pH and dissolved oxygen content of zymotic fluid during acid, so that the accurately process conditions in control production process, keep it in life Under the optimum condition for producing hyaluronic acid, and then improve the yield of hyaluronic acid.

Brief description of the drawings

Fig. 1 is the structural representation of the utility model fermentation tank

In figure：

1st, tank body 2, charge door 3, discharging opening

4th, agitating shaft 5, motor 6, agitating paddle

7th, pH meter 8, dissolved oxygen sensor 9, through hole

10th, pipeline 11, soda acid liquid storage tank 12, liquid feeding pump

13rd, oxygen air-spreading disk 14, air inlet pipe 15, apparatus of oxygen supply

16th, venthole 17, magnetic valve 18, magnetic valve

Embodiment

The utility model embodiment is described further below in conjunction with the accompanying drawings：

As shown in figure 1, a kind of hyaluronic acid production fermentation tank described in the present embodiment, including tank body 1, the bottom of tank body 1 Provided with base, tank body 1 is externally provided with chuck, is exchanged heat for tank body 1, to prevent that rapids occurs in whipping process for zymotic fluid in tank body 1 Stream, is provided with baffle plate on the madial wall of tank body 1, and the top of tank body 1 is provided with charge door 2, and the bottom of tank body 1 is provided with discharging opening 3, tank body 1 Portion is provided with pH meter and dissolved oxygen sensor interface, wherein being inserted with pH meter 7 and dissolved oxygen sensor 8, pH meter 7 and dissolved oxygen sensor 8 are stretched into The middle part upper position of tank body 1, can feed back to operating personnel with the pH and dissolved oxygen content of zymotic fluid in real-time monitoring of tank body, when When the pH or dissolved oxygen content of zymotic fluid deviate optimum value, the environment of zymotic fluid system is adjusted operating personnel, to improve The yield of hyaluronic acid.

Agitating shaft 4 is stretched into tank body 1 by the top of tank body 1, and the top of agitating shaft 4 is connected with the output shaft of motor 5, agitating shaft 4 Bottom connect multiple agitating paddles 6, agitating paddle 6 at least 3, agitating shaft 4 and agitating paddle 6 be cavity body, and agitating shaft 4 Cavity body is connected with the cavity body of agitating paddle 6, and agitating paddle 6 is provided with the through hole 9 communicated with the cavity of agitating paddle 6, the top of agitating shaft 4 End is connected by a pipeline 10 with the soda acid liquid storage tank 11 outside fermentation tank 1, and pipeline 10 is provided with liquid feeding pump 12, saturating in fermenting and producing During bright matter acid, the worth pH for knowing zymotic fluid in tank body 1 that operating personnel are shown by pH meter, cell thalline is in growth course Middle production acid makes pH decline rapidly, when deviate from the optimum value of thalli growth, and operating personnel use soda acid liquid storage tank 11 to fermentation Alkali is added in tank 1, to be adjusted to optimum value, and alkali lye enters the cavity of agitating shaft 4 via pipeline 10, then on agitated oar 6 Through hole 9 discharge into fermentation tank, with the rotation of agitating shaft, alkali lye carries out centrifugal motion, and dispersed release is in fermentation Each position in liquid, the local alkali lye overrich phenomenon that conventional single-point can be avoided to be produced when adding alkali lye, uniform adjustment PH in zymotic fluid, improves the throughput rate of hyaluronic acid.

The bottom of tank body 1 is provided with oxygen air-spreading disk 13, and oxygen air-spreading disk 13 passes through the apparatus of oxygen supply outside air inlet pipe 14 and tank body 1 15 connections, apparatus of oxygen supply 15 can be oxygen tank or air compressor machine, and oxygen air-spreading disk 13 is arranged at the lower section of agitating paddle 6, oxygen The upper side of air-spreading disk 13 is provided with some ventholes 16, radial radiation arrangement of multiple ventholes 16 along oxygen air-spreading disk 13.When When dissolved oxygen sensor 8 detects dissolved oxygen content in zymotic fluid and is less than optimum value, operating personnel are by apparatus of oxygen supply 15 to tank Oxygen supply in body 1, oxygen is introduced into tank body 1 by air inlet pipe 14, and the venthole of the uniform radial radiation arrangement on air-spreading disk 13 16 are discharged into zymotic fluid, and bubble is gradually increasing by the bottom of fermentation tank 1, and are smashed by the agitating paddle 6 of the top of air-spreading disk 13 Into a large amount of tiny bubbles, the contact area of oxygen and zymotic fluid is greatly improved, whole zymotic fluid is uniformly have adjusted Dissolved oxygen amount.

The pipeline 10 being connected with agitating shaft 4 and magnetic valve 17,18 is equipped with the air inlet pipe 14 being connected with air-spreading disk 13, Controller is additionally provided with outside tank body 1, controller is electrically connected with magnetic valve 17,18, pH meter 7 and dissolved oxygen sensor 8 respectively, the He of pH meter 7 Dissolved oxygen sensor 8 sends the pH of the zymotic fluid real-time monitored and dissolved oxygen content signal to controller, controller by signal with The optimum value of setting is compared, when pH is relatively low, and controller output order control magnetic valve 17 is opened, and is added into fermentation tank 1 Alkali lye, adjusts the pH of zymotic fluid, is reached to pH and magnetic valve 17, when dissolved oxygen content is relatively low, controller output order are closed after optimum value Control magnetic valve 18 is opened, and the supplemental oxygen into fermentation tank 1 reaches to dissolved oxygen amount and magnetic valve 18 is closed after optimum value.Pass through control Signal is transmitted between device and pH meter 7 and dissolved oxygen sensor 8, magnetic valve is controlled, fermentation tank can be made to automatically control in real time into alkali lye And oxygen supply, from without manual adjustment, the pH and dissolved oxygen amount of fermentation cylinder for fermentation liquid are automatically adjusted, makes zymotic fluid pH and dissolved oxygen amount Regulation is more timely, improves the yield of hyaluronic acid.

Advantage and beneficial effect of the present utility model are：It can more accurate, uniform, automatically regulate and control production hyaluronic acid mistake The pH and dissolved oxygen content of zymotic fluid in journey, so that the accurately process conditions in control production process, keep it in production saturating Under the optimum condition of bright matter acid, and then improve the yield of hyaluronic acid.

One embodiment of the present utility model is described in detail above, but the content is only of the present utility model Preferred embodiment, it is impossible to be considered as being used to limit practical range of the present utility model.It is all to be made according to present utility model application scope Equivalent change with improve etc., all should still belong within patent covering scope of the present utility model.

Claims (4)

1. a kind of hyaluronic acid production fermentation tank, including tank body, the charge door that is respectively arranged at the top and bottom of the tank body and Discharging opening, the agitating shaft in the tank body and the agitating paddle located at the agitating shaft bottom, it is characterised in that：The tank body Interior to be provided with pH meter and dissolved oxygen sensor, the agitating shaft and the agitating paddle are cavity body, the cavity of the agitating shaft and institute The cavity connection of agitating paddle is stated, the agitating paddle is provided with the through hole communicated with the agitating paddle cavity, the top of the agitating shaft End is connected by pipeline with soda acid liquid storage tank, and the pipeline is provided with liquid feeding pump, and the tank body inner bottom part is provided with oxygen air-spreading disk, The oxygen air-spreading disk is connected by air inlet pipe with the apparatus of oxygen supply outside the tank body, provided with radially on the upside of the oxygen air-spreading disk Radiate the stomata of arrangement.

2. fermentation tank according to claim 1, it is characterised in that：The pipeline is provided with the first magnetic valve, the air inlet Pipe is provided with the second magnetic valve.

3. fermentation tank according to claim 2, it is characterised in that：Also include controller, the controller respectively with it is described PH meter, the dissolved oxygen sensor, first magnetic valve and second magnetic valve electrical connection.

4. according to any described fermentation tanks of claim 1-3, it is characterised in that：The oxygen air-spreading disk is located at the agitating paddle Lower section.