CN215250779U - Microbial cultivation device for microbial functionality experiments - Google Patents
Microbial cultivation device for microbial functionality experiments Download PDFInfo
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- CN215250779U CN215250779U CN202022045007.9U CN202022045007U CN215250779U CN 215250779 U CN215250779 U CN 215250779U CN 202022045007 U CN202022045007 U CN 202022045007U CN 215250779 U CN215250779 U CN 215250779U
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- 238000002474 experimental method Methods 0.000 title claims abstract description 18
- 230000000813 microbial effect Effects 0.000 title abstract description 6
- 244000005700 microbiome Species 0.000 claims abstract description 52
- 238000003756 stirring Methods 0.000 claims abstract description 21
- 230000007246 mechanism Effects 0.000 claims abstract description 12
- 238000005485 electric heating Methods 0.000 claims abstract description 11
- 125000003003 spiro group Chemical group 0.000 claims abstract 2
- 239000007788 liquid Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000035764 nutrition Effects 0.000 claims description 7
- 235000016709 nutrition Nutrition 0.000 claims description 7
- 238000009434 installation Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 13
- 230000008569 process Effects 0.000 abstract description 5
- 238000012136 culture method Methods 0.000 description 15
- 239000001963 growth medium Substances 0.000 description 9
- 238000012258 culturing Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001020 rhythmical effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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Abstract
The utility model discloses a microbial cultivation device for microbial functionality experiments, the power distribution box comprises a box body, open the box both sides has the spout, and spout inner wall sliding connection has the dead lever, threaded hole is opened to dead lever one end, and threaded hole inner wall spiro union has the threaded rod, the threaded rod top is connected with first motor, box both sides inner wall is fixed with four symmetric distribution's support bar, and support bar top sliding connection has the tray. The utility model can provide sterile and suitable-temperature air for the equipment by using the fan and the electric heating wire, can sterilize the equipment at high temperature, and can preheat the culture bottle and improve the suitable-temperature hot air in a short distance by using the spiral stirring rod, so that the microorganism culture efficiency is higher; through using second motor, spiral puddler and vibration mechanism, can stir and shake the microorganism culture process for the microorganism is more abundant with air and culture solution and is contacted, improves the efficiency that the microorganism was cultivateed.
Description
Technical Field
The utility model relates to a microorganism culture apparatus technical field for the microorganism functionality experiment especially relates to a microorganism culture apparatus for microorganism functionality experiment.
Background
The microbial culture method is a method of propagating microorganisms under artificial conditions. Different culture methods can be adopted according to different requirements on the types of microorganisms and environmental conditions such as nutrients, temperature, oxygen, moisture, pH value and the like and the specific requirements on the series production and experiments. The method can be divided into aerobic culture method and anaerobic culture method. One is aerobic microbial culture method, which is commonly used: firstly, a shaking table culture method is adopted, namely, microorganisms are inoculated in a triangular flask filled with a liquid culture medium and then placed on a shaking table in a constant-temperature culture chamber for rhythmic oscillation, so that air continuously enters a culture solution to promote the microorganisms to grow well; secondly, a shallow culture medium is placed in the tray to ensure that microorganisms can fully contact with air to be beneficial to growth and propagation by a shallow tray culture method, also called a surface culture method, but the method needs large space and is easy to pollute mixed bacteria; the deep culture method is suitable for large-scale fermentation culture of aerobic microbe, and can make the microbe contact with air fully and breed and accumulate metabolite in large-volume liquid culture medium by introducing sterile air and stirring continuously. The other is anaerobic microbe culture method, which is a chemical reducing agent or air extractor commonly used in laboratories to absorb molecular oxygen in culture medium, and also is a submerged culture method in a static state. In the production, a sealed fermentation tank or a solid fermentation method without ventilation is generally used. According to the culture process, the method can be divided into the following steps: the intermittent culture method, also called batch culture method, is a culture method in which microorganisms are inoculated in a certain volume of culture medium and harvested once after culture. Continuous culture method, continuously supplementing fresh nutrients to culture medium and discharging culture at the same speed, according to different control modes, dividing it into constant turbidity continuous culture method, i.e. continuously regulating flow rate to make the turbidity of bacterial liquid be constant, and then adopting constant continuous culture method to control constant flow rate to ensure constant growth rate of microbe. For viruses, living animal and plant tissues or ex vivo living tissues are usually used for culturing. When carrying out the heating to the device temperature, the microorganism culture apparatus who uses at present all is through the whole heating to the equipment inside, and the temperature control effect of its culture medium is poor, and is not convenient for to the accurate control of the temperature of microorganism culture medium, and in the air entered equipment inner space entering culture medium at the cultivation in-process simultaneously, easily caused certain pollution risk, the efficiency of cultivateing simultaneously is lower.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a microorganism culture device for microorganism functionality experiments.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a microorganism culture device for microorganism functionality experiments comprises a box body, wherein sliding grooves are formed in two sides of the box body, fixing rods are connected to the inner walls of the sliding grooves in a sliding manner, threaded holes are formed in one ends of the fixing rods, threaded rods are screwed on the inner walls of the threaded holes, a first motor is connected to the top ends of the threaded rods, four symmetrically-distributed support bars are fixed to the inner walls of two sides of the box body, a tray is connected to the top of each support bar in a sliding manner, three culture bottles distributed equidistantly are placed at the top of the tray, a dropping liquid pipe is installed at one side of the top of each tray, a fan is installed in the center of the top of the box body, a mounting groove is formed in the position, below the fan, of the top of the box body, an electric heating wire is fixed to the inner wall of the mounting groove, an air inlet pipe is installed at the position, a second motor is fixed to the upper side of the top of each fixing rod, of each culture bottle, and the dead lever is located the second motor downside and opens there is the fixed slot, the seal box is installed to fixed slot inner wall, the seal box is opened there is the inlet port, and the inlet port inner wall is connected with the intake pipe, the output shaft outer wall of second motor forms to rotate through bearing and seal box and is connected, the output shaft one end of second motor is fixed with the spiral puddler, and has seted up the air inlet duct in the spiral puddler, the air outlet that has a plurality of evenly distributed is opened to the spiral puddler outer wall, and the air outlet is linked together with the air inlet duct, the air inlet duct inner wall is equipped with vibration mechanism, and vibration mechanism includes blade and rubber ball, blade one end is fixed with the spring, and spring one end is at the rubber ball outer wall.
Preferably, the inner wall of the bottom of the box body is connected with a nutrition pool in a sliding mode, a water pump is installed on the inner wall of the bottom of the nutrition pool, and the liquid outlet end of the water pump is connected to the bottom of the liquid dropping pipe.
Preferably, the outer wall of the output shaft of the second motor is provided with a plurality of uniformly distributed mounting holes in the sealing box, the inner wall of the air inlet groove is fixed with a cross, and the center of the cross is in running fit with the blades through a bearing.
Preferably, the size of the spiral stirring rod is smaller than the size of the mouth of the culture bottle, and a thermometer is arranged on the outer wall of one side of the box body.
Preferably, a mounting opening is formed in one end of the box body, and a box door is hinged to the inner wall of one side of the mounting opening through a hinge.
Preferably, the first motor, the second motor, the fan, the water pump and the electric heating wire are all connected with a switch through wires, and the switch is connected with a power supply through a power line.
The utility model has the advantages that:
1. the fan and the electric heating wire can provide sterile and proper-temperature air for the equipment, so that the equipment can be sterilized at high temperature, and the culture bottle can be preheated and the proper-temperature hot air can be increased at a short distance by using the spiral stirring rod, so that the microorganism culture efficiency is higher;
2. through using second motor, spiral puddler and vibration mechanism, can stir and shake the microorganism culture process for the microorganism is more abundant with air and culture solution and is contacted, improves the efficiency that the microorganism was cultivateed.
Drawings
FIG. 1 is a schematic structural view of a microorganism culture apparatus for microorganism functionality experiments according to the present invention;
FIG. 2 is a schematic view of a spiral stirring rod of a microorganism culture apparatus for microorganism functionality experiments according to the present invention;
fig. 3 is a structural schematic diagram of a vibration mechanism of a microorganism culture apparatus for microorganism functionality experiments provided by the utility model.
In the figure: the device comprises a box body 1, a fixing rod 2, a tray 3, a first motor 4, a threaded rod 5, a culture flask 6, a fan 7, an electric heating wire 8, an air inlet pipe 9, a second motor 10, a spiral stirring rod 11, a sealed box 12, a liquid dropping pipe 13, a water pump 14, a nutrition pool 15, a vibration mechanism 16, blades 17 and rubber balls 18.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
Referring to fig. 1-3, a microorganism culture device for microorganism functionality experiments, comprising a box body 1, wherein sliding grooves are formed on two sides of the box body 1, a fixed rod 2 is slidably connected to the inner wall of the sliding groove, a threaded hole is formed at one end of the fixed rod 2, a threaded rod 5 is screwed on the inner wall of the threaded hole, a first motor 4 is connected to the top end of the threaded rod 5, four symmetrically distributed support bars are fixed on the inner wall of two sides of the box body 1, a tray 3 is slidably connected to the top of the support bars, three culture bottles 6 which are equidistantly distributed are placed on the top of the tray 3, a dropping liquid pipe 13 is installed on one side of each culture bottle 6 on the top of the tray 3, a fan 7 is installed at the center of the top of the box body 1, an installation groove is formed on the lower side of the fan 7 on the top of the box body 1, an electric heating wire 8 is fixed on the inner wall of the installation groove, an air inlet pipe 9 is installed on the lower side of the inner wall of the installation groove on the top of the box body 1, sterile and air with proper temperature can be provided for the equipment by using the fan and the electric heating wire, the device can be sterilized at high temperature, and simultaneously, the culture bottle can be preheated in a short distance by utilizing the spiral stirring rod and the hot air with proper temperature is increased, so that the microorganism culture efficiency is higher;
the top of the fixed rod 2 is positioned on the upper side of the culture bottle 6 and is fixed with a second motor 10, the fixed rod 2 is positioned on the lower side of the second motor 10 and is provided with a fixed groove, the inner wall of the fixed groove is provided with a seal box 12, the seal box 12 is provided with an air inlet, the inner wall of the air inlet is connected with an air inlet pipe 9, the outer wall of an output shaft of the second motor 10 is rotatably connected with the seal box 12 through a bearing, one end of the output shaft of the second motor 10 is fixed with a spiral stirring rod 11, an air inlet groove is arranged in the spiral stirring rod 11, the outer wall of the spiral stirring rod 11 is provided with a plurality of air outlet holes which are uniformly distributed, the air outlet holes are communicated with the air inlet groove, the inner wall of the air inlet groove is provided with a vibration mechanism 16, the vibration mechanism 16 comprises a blade 17 and a rubber ball 18, one end of the blade 17 is fixed with a spring, one end of the spring is positioned on the outer wall of the rubber ball 18, through the use of the second motor, the spiral stirring rod and the vibration mechanism, the stirring and the vibration can be carried out in the process of culturing the microorganisms, so that the microorganisms are more fully contacted with the air and the culture solution, and the efficiency of culturing the microorganisms is improved;
inner wall sliding connection has nutrition pond 15 bottom box 1, and nutrition pond 15 bottom inner wall installs water pump 14, the play liquid end of water pump 14 is connected in dropping liquid pipe 13 bottom, the output shaft outer wall of second motor 10 is located and has seted up a plurality of evenly distributed's mounting hole in seal box 12, the air inlet duct inner wall is fixed with the cross, and cross central point puts and forms normal running fit through bearing and blade 17, the size of spiral puddler 11 will be less than 6 bottleneck sizes of blake bottle, the thermometer is installed to 1 one side outer wall of box, 1 one end of box is opened there is the installing port, and the inner wall of installing port one side articulates through the hinge has the chamber door, first motor 4, second motor 10, fan 7, water pump 14 and electric heating wire 8 all are connected with the switch through the wire, and the switch is connected with the power through the power cord.
The working principle is as follows: the fan 7 and the electric heating wire 8 can provide sterile and proper-temperature air for the equipment, so that the equipment can be sterilized at high temperature, and meanwhile, the culture bottle 6 can be preheated in a short distance by using the spiral stirring rod 11 and the proper-temperature hot air is increased, so that the microorganism culture efficiency is higher; through using second motor 10, spiral puddler 11 and vibration mechanism 16, can stir and vibrations to the microorganism cultivation process for the microorganism more fully contacts with air and culture solution, improves the efficiency that the microorganism was cultivateed, through utilizing tray 3 to conveniently prevent blake bottle 6 more, can adjust spiral puddler 11 from top to bottom through utilizing first motor 4 and threaded rod 5, conveniently stir more.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (6)
1. The utility model provides a microorganism culture apparatus for microorganism functionality experiments, includes box (1), its characterized in that, open box (1) both sides has the spout, and spout inner wall sliding connection has dead lever (2), threaded hole is opened to dead lever (2) one end, and threaded hole inner wall spiro union has threaded rod (5), threaded rod (5) top is connected with first motor (4), box (1) both sides inner wall is fixed with four symmetric distribution's support bar, and support bar top sliding connection has tray (3), three equidistance distribution's blake bottle (6) have been placed at tray (3) top, and tray (3) top is located blake bottle (6) one side and installs dropping liquid pipe (13), box (1) top central point puts and installs fan (7), and box (1) top is located fan (7) downside and opens there is the mounting groove, the culture flask is characterized in that an electric heating wire (8) is fixed on the inner wall of the mounting groove, an air inlet pipe (9) is arranged at the top of the box body (1) and positioned at the lower side of the inner wall of the mounting groove, a second motor (10) is fixed at the top of the fixing rod (2) and positioned at the upper side of the culture flask (6), a fixing groove is formed at the lower side of the second motor (10), a sealing box (12) is arranged on the inner wall of the fixing groove, an air inlet hole is formed in the sealing box (12) and connected with the air inlet pipe (9), the outer wall of an output shaft of the second motor (10) is rotatably connected with the sealing box (12) through a bearing, a spiral stirring rod (11) is fixed at one end of the output shaft of the second motor (10), an air inlet groove is formed in the spiral stirring rod (11), a plurality of air outlet holes which are uniformly distributed are formed in the outer wall of the spiral stirring rod (11), and the air outlet holes are communicated with the air inlet groove, the air inlet groove inner wall is equipped with vibration mechanism (16), and vibration mechanism (16) include blade (17) and rubber ball (18), blade (17) one end is fixed with the spring, and spring one end is at rubber ball (18) outer wall.
2. The microorganism culture device for the microorganism functionality experiment as claimed in claim 1, wherein the inner wall of the bottom of the box body (1) is slidably connected with a nutrition pool (15), a water pump (14) is installed on the inner wall of the bottom of the nutrition pool (15), and the liquid outlet end of the water pump (14) is connected to the bottom of the dropping liquid pipe (13).
3. The microorganism culture device for the microorganism functionality experiment according to claim 1, wherein the outer wall of the output shaft of the second motor (10) is provided with a plurality of uniformly distributed mounting holes in the sealing box (12), the inner wall of the air inlet groove is fixed with a cross, and the center position of the cross forms a rotating fit with the blades (17) through a bearing.
4. The microorganism culture device for microorganism functionality experiments as claimed in claim 1, wherein the size of the spiral stirring rod (11) is smaller than the size of the mouth of the culture bottle (6), and a thermometer is arranged on the outer wall of one side of the box body (1).
5. The microorganism culture device for microorganism functionality experiments as claimed in claim 1, wherein one end of the box body (1) is provided with an installation opening, and the inner wall of one side of the installation opening is hinged with a box door through a hinge.
6. The microorganism culture device for microorganism functionality experiments according to claim 2, characterized in that the first motor (4), the second motor (10), the blower (7), the water pump (14) and the electric heating wire (8) are all connected with a switch through wires, and the switch is connected with a power supply through a power line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022045007.9U CN215250779U (en) | 2020-09-17 | 2020-09-17 | Microbial cultivation device for microbial functionality experiments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022045007.9U CN215250779U (en) | 2020-09-17 | 2020-09-17 | Microbial cultivation device for microbial functionality experiments |
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| Publication Number | Publication Date |
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| CN215250779U true CN215250779U (en) | 2021-12-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022045007.9U Expired - Fee Related CN215250779U (en) | 2020-09-17 | 2020-09-17 | Microbial cultivation device for microbial functionality experiments |
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| Country | Link |
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| CN (1) | CN215250779U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115747041A (en) * | 2022-11-10 | 2023-03-07 | 西安工程大学 | A test device for microbial degradation method restores oil-bearing soil |
-
2020
- 2020-09-17 CN CN202022045007.9U patent/CN215250779U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115747041A (en) * | 2022-11-10 | 2023-03-07 | 西安工程大学 | A test device for microbial degradation method restores oil-bearing soil |
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| GR01 | Patent grant | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211221 |