CN201850276U - Micro-filtration membrane coupling high-density culture device for lactic acid bacteria - Google Patents
Micro-filtration membrane coupling high-density culture device for lactic acid bacteria Download PDFInfo
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- CN201850276U CN201850276U CN2010201831809U CN201020183180U CN201850276U CN 201850276 U CN201850276 U CN 201850276U CN 2010201831809 U CN2010201831809 U CN 2010201831809U CN 201020183180 U CN201020183180 U CN 201020183180U CN 201850276 U CN201850276 U CN 201850276U
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Abstract
The utility model relates to the manufacturing field of a microorganism high-density culture device and discloses a micro-filtration membrane coupling high-density culture device for lactic acid bacteria. The micro-filtration membrane coupling high-density culture device for the lactic acid bacteria comprises a fermentation tank (1), a material supplement tank (2), an air sterilization and filtration system, a steam sterilization system, a temperature control system, a pH measurement and control system, a dissolved oxygen measurement system, an electric control cabinet and a membrane filtration system, wherein the membrane filtration system comprises a hollow fiber membrane device (3) and a peristaltic pump (9); a circulation port of the hollow fiber membrane device (3) is connected with a feeding port of the fermentation tank (1) through a pipeline; a feeding port of the hollow fiber membrane device (3) is connected with a discharging port of the fermentation tank (1) through a pipeline; and a metabolite discharging port of the hollow fiber membrane device (3) is connected with a dirt discharge port though a pipeline. The micro-filtration membrane coupling high-density culture device for the lactic acid bacteria improves the conversion rate of substrates and is suitable for the experimental research and the production application of the high-density culture for the lactic acid bacteria.
Description
Technical field
The utility model relates to a kind of microorganism high-density culture device and makes the field, relates in particular to the device that utilizes microfiltration membrane coupling bio-reactor high-density culture lactic acid bacteria for kraut.
Background technology
China's tradition pickles generally adopt natural fermentating process, have fermentation period length, be subjected to seasonal effect, easily are bacterial contamination and drawbacks such as nitrite exceeds standard, particularly wherein milk-acid bacteria content is low, can't satisfy the commercial scale production requirement, simultaneously, it is stable that the kimchi products quality also is difficult to keep.Being developed as of throw type leaven realized the pickles suitability for industrialized production and guaranteed that its edible safety opened up new way, and the research and development of high-density cultivation method of milk-acid bacteria and equipment are the gordian techniquies that throw type leaven is produced.
It is that cultured continuously is combined with the micro-filtration technology that the microfiltration membrane coupling is cultivated, an additional cover membrane separation unit on common culture apparatus.Make nutrient solution flow through filtering membrane with pump thalline is held back, filtrate comprises that the product in the culturing process flows out culture system together, and adds fresh substratum by liquid level meter control, and it is constant to keep culture volume.Lactic acid is removed fully in the membrane filtration culturing process, is one of present comparatively ideal microorganism high-density cultivation method.
Prior art has recognized that with application of membrane in the milk-acid bacteria high-density culture, its effect is better than the traditional zymotic mode, and in feeding culture, membrane filtration technique can separate the removal meta-bolites, makes the milk-acid bacteria high-density culture.
Suzuki was 141g/L with the maximum bacterium dry weight that ceramic membrane obtains in 1996, and its productive rate is about 0.8g/, and (L * h), water loss is about 0.2m
3/ kg.Korea Spro in 2006 also dragon has carried out the research of membrane filtration formula milk-acid bacteria high-density culture, adopts ceramic micro filter membrane concentration and reactor coupling mutually, the biochemical reactor of the milk-acid bacteria high-density culture of designing.But there is the equipment manufacturing cost height in the ceramic membrane that above institute uses, film fragility is big and the easy defective such as obstruction of film.
Wang's grace in 1989 is great to make milk-acid bacteria concentration reach 17 times of batch culture with the home-made hollow-fibre ultrafiltration device, though this research has significantly improved cell concentration, does not consider the production concentration in the actual production process, factors such as conversion of substrate.This technology adopts homemade hollow-fibre membrane, and with the 0.1%NaOCl sterilization, sterilized water fully washes before using, and has the halfway defective of sterilization, and can't realize the permanently effective running and the regeneration of filtration unit.
In addition, the factor that influences high-density culture also has: pH value, feed supplement mode and the nutrient solution rheological properties etc. of the accumulation of amicine, dissolved oxygen concentration, culture temperature, nutrient solution in the required nutritive substance of thalli growth, the culturing process.
For this reason, the utility model is incorporated into membrane technique in the lactic acid bacteria for kraut high-density culture process, utilize a series of transmitter strictnesses of bio-reactor to control fermenting process well, designed that a nested structure is simple, easy to use, level of automation is high and be suitable for the device of milk-acid bacteria high-density culture.
Summary of the invention
The purpose of this utility model is to overcome the above-mentioned defective of traditional zymotic and prior art existence, a kind of device of hollow fiber microfiltration membrane coupling bio-reactor high-density culture lactic acid bacteria for kraut is provided, utilize microbial fermentation bio-reactor and membrane technique to combine, meta-bolites is shifted out, thereby removed the restraining effect in the lactobacter growth, so both guaranteed the activity of milk-acid bacteria, and can make the continuous enrichment of thalline again.
Technical solution adopted in the utility model is that the utility model mainly is made of bio-reaction system and membrane filtration system two major parts:
Bio-reaction system: partly form by fermentor tank, feed supplement jar, air sterilization and filtering system, steam sterilizing system, temperature controlling system, pH measurement and Controlling System, dissolved oxygen sensing system, electrical control cabinet etc.Wherein fermentor tank by material-transporting system, froth breaking system, DO value control system, pH Controlling System and temperature control system independently five parts form.Whole bio-reaction system adopts the PLC design, controls whole fermentation process automatically by the parameter setting on the operating panel.
Membrane filtration system: comprise hollow fiber membrane device and peristaltic pump, hollow fiber membrane device is for being provided with a branch of hollow fiber bundle in upright shell, the two ends of every film pipe are fixed with tamanori and putamina body and inwall adhesion, the upper end is provided with thalline circulation port and metabolite relief outlet, the lower end is provided with opening for feed, under certain pressure effect, liquid enters film device from bottom to top through opening for feed, small-molecule substance sees through and is arranged in hollow-fibre membrane tube wall micropore permeation on every side to tube wall, flow out through the metabolite relief outlet then, and be trapped in macromolecular substance in the hollow-fibre membrane, can derive by the loop exit of offering on putamina body one side.
The circulation port of hollow fiber membrane device links to each other with the opening for feed of fermentor tank by pipeline, and pipeline is provided with feed valve A, and feed valve A is a needle type valve; The opening for feed of described hollow fiber membrane device links to each other with the discharge port of described fermentor tank by pipeline, is provided with peristaltic pump, bleeder valve E on the pipeline successively; The metabolite relief outlet of described hollow fiber membrane separation device is discharged waste by pipe connection from sewage draining exit, pipeline is provided with wash water valve E.
Described fermentor tank, feed supplement jar are cylindric device high temperature resistant, corrosion resistant stainless steel, this container head sealing, various annexes wherein are housed, for example valve, pipeline and be used for measuring probe and other utility appliance, electrical control cabinet is positioned at the fermentor tank next door.Steam system, air system and water circuit system adopt valve control respectively, and water circuit system is mainly used in the cleaning and the constant temperature water tank water inlet of fermentor tank; Vapor system is used for the disinfection of whole device to be handled; Air system is mainly used in aerated culture and reinforced voltage supply.Standby various transmitters are beneficial to omnidistance control in the fermenting process on the instrument.Material is squeezed into fermentor tank through peristaltic pump, and product directly shifts out from the lower end discharge port, and convenient loading and unloading of leaching material as wear ring, guarantee the resistance to air loss of system with the high-temperature-resisting silicon rubber material.Described thermostat container is the cylindrical or rectangular devices of stainless steel, is furnished with a heating tube in it, is used to keep water temperature constant, and thermostat container one end links to each other with water pump, and the other end enters in the fermentor tank interlayer by constant-temperature water valve.For ease of the automatic control of work, this device also is provided with temperature, pH value, dissolved oxygen amount transmitter at fermentor tank, feed supplement jar, and fermentor tank can be designed to various volumes according to actual needs, and other parts are configured to corresponding size or specification by the zymotechnique requirement.
The working process of this device is as follows:
(1) before equipment uses, should check earlier whether power supply is normal, air compressor machine, vapour generator, whether circulating water system can works better.Whether the valve on the check system, joint and trip bolt are tightened.Start air compressor machine, use 0.15Mpa pressure, check whether the sealing property of fermentor tank, strainer, pipeline, valve is good, have or not leakage.And dissolved oxygen meter, pH instrument, PLC system checked, proofread and correct.
(2) before feeding intake, reply equipment carries out the sky processing that disappears, and gas circuit, material road, fermentor tank, feed supplement jar must be sterilized with steam, eliminate the assorted bacterium at all dead angles, and the system of assurance is in sterile state.When sky disappeared, dissolved oxygen, pH electrode should take out, and can prolong its work-ing life.
(3) during the airline sky disappears process, should close the reducing valve that dewaters, make steam enter total sterilizing filter then by the steam total filter, the vent valve of sterilizing filter lower end should be opened slightly simultaneously, gets rid of water of condensation.Fermentor tank carries out sky when disappearing, should be with the inoculation mouth on the jar, and vent valve, and material road valve opens slightly, makes steam pass through these valves and discharges.
(4) after sky disappears and finishes, in the joint in the tank body of packing into after at first needing pH, DO electrode proofreaied and correct, in then the substratum for preparing being added jar from charging opening, it is carried out reality disappear processing, chuck planted agent no-cooling-water at this moment with steam.
(5) in order to reduce steam condensate, disappear in fact and utilize the logical steam of chuck that substratum is carried out preheating earlier, retaining clip cover pressure≤0.1Mpa, treat that the substratum temperature arrives 90 ℃ after, close jacket steam, change into and directly in jar, feed steam.
(6) rise to 0.12Mpa when tank pressure, when temperature was raised to 121 ~ 123 ℃, control steam valve aperture kept tank pressure constant, stops steam supply after 30 minutes.Open the inlet and discharge valve door of water coolant, water flowing cooling in chuck.
(7) fermenting process is by parameters such as PLC design temperature, pH values, and fermentor tank can be finished whole fermentation process automatically.
(8) after fermentation for some time, fermented liquid is squeezed into membrane filter appts by pump system, meta-bolites oozes out filter membrane and is excluded outward, thalline is circulated again enters fermentor tank, the tank pressure that utilizes air compressor machine to cause simultaneously continues to add fresh substratum and carries out enrichment culture, and recirculation gets final product for 2～3 times like this.
(9) treat that fully fermenting finishes after, fermented liquid is discharged from discharge pipe.
(10) take out dissolved oxygen, pH electrode after the discharging, carry out cleaning maintenance.Discharge water immediately simultaneously and clean fermentor tank and material road pipeline valve, and start air compressor machine, stir, the fermented liquid in the pipeline is rinsed well to the fermentor tank air feed.
The utility model is to have utilized microfiltration membrane coupling milk-acid bacteria high-density culture biological respinse, at first substratum is squeezed into fermentor tank under the effect of peristaltic pump, in the feed supplement jar, after the real processing that disappears, feed cold water cooling substratum, configure the temperature of fermentation cashier's office in a shop in electrical control, after the pH value, promptly finish the whole fermentation process of milk-acid bacteria automatically, temperature in the fermentation is finished by constant temperature water tank water inlet water exhaust system, and by the tunnings such as lactic acid that produce in the automatic interpolation alkali lye and in the fermenting process to keep the constant of pH value, can reach the high-density growth of milk-acid bacteria like this, to improve the milk-acid bacteria productive rate of unit volume.At any time monitor course of fermentation by the DO value control system simultaneously.After fermentation ends, enter fermenting culture in the film device from bottom to top, utilize promptly the centrifugation of film that most of metabolite is exosmosed to enter that film is outer to be moved out of by the metabolite relief outlet, when discharging the milk-acid bacteria meta-bolites, removed restraining effect to lactobacillus-fermented, macromolecular milk-acid bacteria thalline is dammed in film down simultaneously, thereby makes milk-acid bacteria obtain further concentrating, and the loop exit through the film device upper end enters fermentor tank once more and carries out the thalline enrichment.Open air compressor then, filtrated air is fed in the feed supplement jar, utilize pressure reduction, fresh culture is added continued fermentation in the fermentor tank, repetitive operation is 2-3 time like this, makes cell concentration can improve 2 orders of magnitude.
The utility model beneficial effect is:
(1) the membrane concentration assembly of microfiltration membrane coupling bio-reactor of the present utility model adopts hollow-fibre membrane, has overcome defectives such as ceramic membrane price height, the big and easy obstruction of fragility.
(2) hollow-fibre membrane of the present utility model can acidproof, alkaline-resisting and high temperature, can carry out online sterilization, has overcome the existing hollow-fibre membrane halfway defective of sterilizing, and has realized the permanently effective running and the regeneration of filtration unit.
(3) microfiltration membrane coupling bio-reactor of the present utility model adopts a plurality of transmitters and PLC Controlling System such as dissolved oxygen, temperature, pH, to processing parameter such as pH value, feed supplement mode and the nutrient solution rheological properties of the accumulation of amicine in thalli growth desired nutritional material, the culturing process, dissolved oxygen concentration, culture temperature, nutrient solution with fermenting process is strict controls.Save fermentation time, improved cell concentration and conversion of substrate.
(4) the utility model adopts membrane separation technique to replace traditional centrifugation to come enrichment to concentrate thalline, has reduced the rate of loss of thalline, supports for the commercialization production that realizes the agent of efficient concentrated type pickle fermentation provides technology and equipment.And can make the experimental installation or the pilot plant of different scales as required.
(5) the utility model device adopts the tubular fibre membrane technique to reduce apparatus cost significantly, reduce equipment volume, reduction equipment drops into, and running cost is cheap, fractional yield is big, the concentration of milk-acid bacteria is improved 2～3 orders of magnitude, be suitable for milk-acid bacteria high-density culture experimental study and production application.
Description of drawings
Fig. 1 is the utility model apparatus structure synoptic diagram.
Among the figure 1, fermentor tank; 2, the feed supplement jar; 3, hollow fiber membrane device; 4, filtering pressure reducer; 5, total filter; 6, fine filter A; 7, fine filter B; 8, steam filter; 9, peristaltic pump; 10, constant temperature water tank; 11, water pump; 12, steam orifice; 13, water-in; 14, sewage draining exit; 15, magnetic valve A; 16, magnetic valve B; 17, wash water valve A; 18, wash water valve B; 19, wash water valve C; 20, wash water valve D; 21, wash water valve E; 22, bleeder valve D; 23, bleeder valve C; 24, bleeder valve A; 25, bleeder valve B; 26, water intaking valve A; 27, water intaking valve B; 28, pressure lock A; 29, pressure lock B; 30, pressure lock C; 31, pressure lock D; 32, pressure lock E; 33, pressure lock G; 34, pressure lock F; 35, steam valve A; 36, steam valve B; 37, steam valve C, 38, steam valve D; 39, steam valve E; 40, steam valve H; 41, steam valve F; 42, steam valve G; 43, exhaust-valve A; 44, exhaust-valve B; 45, exhaust-valve C; 46, exhaust-valve D; 47, exhaust-valve E; 48, exhaust-valve F; 49, exhaust-valve G; 50, exhaust-valve J; 51, exhaust-valve H; 52, exhaust-valve I; 53, feed valve A; 54, bleeder valve E; 55, the compressed air inlet; 56, opening for feed; 57, loop exit.
Embodiment
This device mainly is made of bio-reaction system and membrane filtration system two major parts:
Bio-reaction system: partly form by fermentor tank 1, feed supplement jar 2, air sterilization and filtering system, steam sterilizing system, temperature controlling system, pH measurement and Controlling System, dissolved oxygen sensing system, electrical control cabinet etc.Wherein fermentor tank by material-transporting system, froth breaking system, DO value control system, pH Controlling System and temperature control system independently five parts form.Whole bio-reaction system adopts the PLC design, controls whole fermentation process automatically by the parameter setting on the operating panel.
Membrane filtration system: comprise hollow fiber membrane device 3 and peristaltic pump 9, hollow fiber membrane device 3 is for being provided with a branch of hollow fiber bundle in upright shell, the two ends of every film pipe are fixed with tamanori and putamina body and inwall adhesion, the upper end is provided with thalline circulation port and metabolite relief outlet, the lower end is provided with opening for feed, under certain pressure effect, liquid enters film device from bottom to top through opening for feed, small-molecule substance sees through and is arranged in hollow-fibre membrane tube wall micropore permeation on every side to tube wall, flow out through the metabolite relief outlet then, and be trapped in macromolecular substance in the hollow-fibre membrane, can derive by the loop exit of offering on putamina body one side.
Claims (3)
1. the microfiltration membrane coupling high-density culture device of milk-acid bacteria, comprise fermentor tank (1), feed supplement jar (2), air sterilization and filtering system, steam sterilizing system, temperature controlling system, pH measurement and Controlling System, dissolved oxygen sensing system, electrical control cabinet, it is characterized in that, also comprise membrane filtration system, described membrane filtration system comprises hollow fiber membrane device (3) and peristaltic pump (9); The thalline loop exit (57) of described hollow fiber membrane device (3) links to each other by the opening for feed of pipeline with described fermentor tank (1), and pipeline is provided with feed valve A (53); The opening for feed (56) of described hollow fiber membrane device (3) links to each other by the discharge port of pipeline with described fermentor tank (1), is provided with peristaltic pump (9), bleeder valve E (54) on the pipeline successively; The metabolite relief outlet of described hollow fiber membrane separation device (3) is by pipe connection sewage draining exit (14), and pipeline is provided with wash water valve E (21).
2. the microfiltration membrane coupling high-density culture device of milk-acid bacteria according to claim 1, it is characterized in that, described hollow fiber membrane device (3) is for being provided with a branch of hollow fiber bundle in upright shell, fixedly connected with the upright shell inwall with tamanori in the two ends of every film pipe, the upper end is provided with thalline loop exit (57), the lower end is provided with opening for feed (56), offers the metabolite relief outlet on upright shell one side.
3. the microfiltration membrane coupling high-density culture device of milk-acid bacteria according to claim 1 and 2 is characterized in that, described feed valve A (53) and described bleeder valve E (54) are needle type valve, and described wash water valve E (21) is a spherical valve.
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| CN2010201831809U CN201850276U (en) | 2010-05-07 | 2010-05-07 | Micro-filtration membrane coupling high-density culture device for lactic acid bacteria |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321537A (en) * | 2011-08-31 | 2012-01-18 | 天津大学 | Bubbling bioreactor used for plant tissue culture |
| CN102533540A (en) * | 2011-12-29 | 2012-07-04 | 天津工业生物技术研究所 | Accurate measurement fed-batch device for fermentation tank and fed-batch method for high-concentration solution |
| CN102994380A (en) * | 2012-12-31 | 2013-03-27 | 光明乳业股份有限公司 | Preparation process of concentrated lactic acid bacteria starter and special device thereof |
| CN103497890A (en) * | 2013-09-26 | 2014-01-08 | 北京华都诗华生物制品有限公司 | Perfusion culture system and method for bacteria fermentation tank |
| WO2018214317A1 (en) * | 2017-05-25 | 2018-11-29 | 广州无添加主义化妆品有限公司 | Bio-fermentation filtration system |
| CN109363119A (en) * | 2018-11-21 | 2019-02-22 | 中国农业科学院麻类研究所 | Utilize the method and pickle jar of pickles direct putting type microbial inoculum production pickles |
| CN113789253A (en) * | 2021-09-30 | 2021-12-14 | 四川省食品发酵工业研究设计院有限公司 | High-density fermentation system and fermentation method for lactic acid bacteria |
| CN114134187A (en) * | 2021-11-19 | 2022-03-04 | 江西诚志生物工程有限公司 | Process for producing pyrroloquinoline quinone disodium salt by fermentation method |
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2010
- 2010-05-07 CN CN2010201831809U patent/CN201850276U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321537A (en) * | 2011-08-31 | 2012-01-18 | 天津大学 | Bubbling bioreactor used for plant tissue culture |
| CN102321537B (en) * | 2011-08-31 | 2013-08-07 | 天津大学 | Bubbling bioreactor used for plant tissue culture |
| CN102533540A (en) * | 2011-12-29 | 2012-07-04 | 天津工业生物技术研究所 | Accurate measurement fed-batch device for fermentation tank and fed-batch method for high-concentration solution |
| CN102533540B (en) * | 2011-12-29 | 2014-01-22 | 天津工业生物技术研究所 | Accurate measurement fed-batch device for fermentation tank and fed-batch method for high-concentration solution |
| CN102994380A (en) * | 2012-12-31 | 2013-03-27 | 光明乳业股份有限公司 | Preparation process of concentrated lactic acid bacteria starter and special device thereof |
| CN103497890A (en) * | 2013-09-26 | 2014-01-08 | 北京华都诗华生物制品有限公司 | Perfusion culture system and method for bacteria fermentation tank |
| WO2018214317A1 (en) * | 2017-05-25 | 2018-11-29 | 广州无添加主义化妆品有限公司 | Bio-fermentation filtration system |
| CN109363119A (en) * | 2018-11-21 | 2019-02-22 | 中国农业科学院麻类研究所 | Utilize the method and pickle jar of pickles direct putting type microbial inoculum production pickles |
| CN113789253A (en) * | 2021-09-30 | 2021-12-14 | 四川省食品发酵工业研究设计院有限公司 | High-density fermentation system and fermentation method for lactic acid bacteria |
| CN113789253B (en) * | 2021-09-30 | 2024-04-09 | 四川省食品发酵工业研究设计院有限公司 | Lactic acid bacteria high-density fermentation system and fermentation method |
| CN114134187A (en) * | 2021-11-19 | 2022-03-04 | 江西诚志生物工程有限公司 | Process for producing pyrroloquinoline quinone disodium salt by fermentation method |
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Granted publication date: 20110601 Termination date: 20140507 |