CN106072582A - A kind of production method of ferment and application thereof - Google Patents
A kind of production method of ferment and application thereof Download PDFInfo
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Abstract
The present invention relates to field of food, particularly relate to production method of a kind of ferment and application thereof.Take fresh Xinhui citrus reticulata meat, squeeze the juice, load in fermentation tank, loading amount is the 96% of total measurement (volume), sealing, at the ozone of the 0.3 0.5% of oxygen-containing space injection total volume of air, the aerobic bacteria being active in liquid surface will touch ozone and be destroyed and reach to eliminate the purpose of aerobic bacteria；Standing for fermentation 24 months, terminates fermentation when pH value point is 2.6 3.6.As long as the 90 days time fermented again after present invention inoculation i.e. can reach, the normal fermentation effect of 720 days can be reached；The present invention principle (method) by space disinfection sterilizing, only at aerobic space input space biocide, ozone, it is possible to effectively kills the aerobic bacteria in surface existence, and remains anaerobe；Sugar part of the present invention preparation is just right；Oxygen-containing space, less than the 5% of total measurement (volume), can well carry out relative anaerobic and control to be adjusted；The present invention accomplishes to terminate fermentation at any time simultaneously.
Description
Technical field
The present invention relates to field of food, particularly relate to production method of a kind of ferment and application thereof.
Background technology
There is numerous ferment products in the market, including import, Japan, Taiwan.Korea S and domestic multiple ferment produce
Product, expensive, very different dragons and fishes jumbled together, brings puzzled and deception to consumers in general.Puzzled main cause is: state
Family is currently without the unified standard of this series products, and mostly with [People's Republic of China's fruit drink standard] import, this is just
Create contradiction: there is strict regulation in beverage made of fruits or vegetables country, particularly has strict restriction to microorganism, so this series products exists
Mostly use high temperature sterilize to process technique before and after the fill of product and carry out production and processing, and high temperature sterilize not only can knock out institute
Some microorganisms, also make ferment (enzyme) inactivate simultaneously.So just creating the universal phenomenon of ferment product in the market:
It not that microorganism exceeds standard, otherwise ferment (enzyme) does not has vigor.What so composition of ferment is actually: use which type of technique
Reach these beneficiating ingredients not to be destroyed, be only the key point producing ferment: as the term suggests, ferment is exactly with fruit and vegetables
The metabolite that dish produces through biofermentation is with the enzyme (the sour molten albumen) mixture as main component, big in its composition with the exception of this
Part is aminoacid, vitamin, mineral and the little peptide of trace element, polysaccharide goods and materials, then the fermentation of ferment is the most scabrous
It is: the generation of miscellaneous bacteria to be controlled that the method generally controlling miscellaneous bacteria is to equipment, and substrate carries out strict sterilization process, adds air
Heat sterilization operates and uses automatic mode to ferment as far as possible.If sweat pollutes miscellaneous bacteria or phagocytosis
Know from experience the carrying out affecting sweat, cause the yield of fermented product to reduce, serious, result even in sweat failure,
Fermented product is required all to outwell, and ferment ferments, and everything all has no way of carrying out, as a example by Xinhui citrus reticulata: the results season of Xinhui citrus reticulata
Joint is annual late December early October, and locality either grower or buyer are intended to skin and do not want meat, thus
Citrus chachiensis Hort. meat not can be caused by careless disposal, and to shine into pollution: 1, injure the peplos of sarcocarp during peeling, fruit juice flows out leads
Cause to pollute.2, container is not clean or Reusability causes polluting, and 3, the time, the sarcocarp after peeling is piled up extruding and is had a lot of fruit juice
Flowing out, oxidized corruption, 4, juice-extracting process control improper causing pollutes.5, do not have timely sterilizing to cause pollution etc. after tinning.
It is can not be by high temperature sterilize because high temperature can cause the original enzyme of fruit and vegerable to inactivate during the fermentation of what is more important fruit and vegerable.
Research shows, the Citrus chachiensis Hort. meat collected at Gan Yuan or square has into the pollution that degree is different, mould through microbe colony sum
The detection of bacterium, total number of molds is both greater than 1.76cfu/ml × 106, and escherichia coli are positive, and test shows escherichia coli and mycete
It is all aerobic Pseudomonas, say, that can effectively kill the aerobic bacteria in liquid and just can remove most harmful bacteria, it is simply that
Say Xinhui citrus reticulata meat ferment to be produced, then must carry out eliminating the process of aerobic bacteria after tinning of squeezing the juice, traditional sterilizing methods is
Enzyme at high temperature sterilizing so fruit juice is the most destroyed, and the inoculation after fermentation cycle is long, and the total amount of enzyme is low, and total class is few.That
A kind of method finding room temperature sterilizing can effectively remove again aerobic bacteria reservation anaerobe in the case of not injuring existing enzyme
It it is exactly one of most important condition producing Xinhui citrus reticulata ferment；The two of condition are: cultivate a kind of yield of enzyme high energy at pH value 2.6-3.6
Environment has the beneficial flora of stronger vitality to improve the quality of ferment；The three of condition, when enzyme values total amount reaches peak
Sterilizing the most at normal temperatures can terminate continuing fermentation and the method that do not injures the vigor of enzyme, possessing these three condition can produce
Safe and reliable ferment product comes.But reach as above purpose currently without suitable technological means simultaneously.
Summary of the invention
The purpose of invention: in order to provide production method of the more preferable ferment of a kind of effect and application thereof, specific purposes are shown in tool
Multiple substantial technological effects of body implementation section.
In order to reach as above purpose, the present invention adopts the following technical scheme that:
Scheme one:
The production method of a kind of ferment, it is characterised in that comprise the steps of,
Taking fresh Xinhui citrus reticulata meat, squeeze the juice, load in fermentation tank, loading amount is the 96% of total measurement (volume), sealing, injects sky in oxygen-containing space
The ozone of the 0.3-0.5% of gas cumulative volume, the aerobic bacteria being active in liquid surface will touch ozone and be destroyed and reach to disappear
Go out the purpose of aerobic bacteria；
Standing for fermentation 24 months, terminates fermentation when pH value point is 2.6-3.6；Flora the most therein includes at least cheese milk bar
One or more in bacterium, the secondary dry bacterium of cheese milk, Lactobacillus brevis, acetobacter, are isolated as fermented bacterium by flora standby
With.
The further technical scheme of the present invention is, also comprises the step adjusting pol, described tune sugar before loading in fermentation tank
The step of degree refers to, adds sucrose 7-5% when measuring Xinhui citrus reticulata juice pol and being 6-9%, and being configured to total pol is 11-13%'s
Material.
The further technical scheme of the present invention is, strain separating the most also comprises the step of amplification culture, described expansion
Big cultivation refers to, takes fresh Xinhui citrus reticulata meat, squeezes the juice, and loads in fermentation tank, and loading amount is the 96% of total measurement (volume), stays 4% space；
Add sucrose 7-5% when measuring Xinhui citrus reticulata juice pol and being 6-9%, be configured to the material that total pol is 11-13%；
Input accounts for the ozone of material ullage space 0.3%, knocks out aerobic flora；Remove miscellaneous bacteria；
Then the flora separated, sealing is inoculated by total amount 1-10% of fermentation liquid；
Standing for fermentation 3 months at normal temperatures；
Terminate fermentation when pH value point is 2.6-3.6, stop fermentation in time.
The further technical scheme of the present invention is, described prevention fermentation refers to input cold antibacterial dimethyl two carbonate
In tank body, i.e. can reach keep-alive and terminate the effect of fermentation；Input concentration is 0.1% according to the total liquid quality of tank interior.
The further technical scheme of the present invention is, also comprises the step inducing fermented bacterium, and described induction is
Refer to, after using ultraviolet and nitrosoguanidine complex mutation, it is thus achieved that sour molten albumen (enzyme) Producing Strain group of hill.
The further technical scheme of the present invention is, the process of fresh Xinhui citrus reticulata meat also comprises roguing removing the necrotic tissue, goes pesticide residues, pouring
Wash, be dehydrated, squeeze the juice filter step.
The further technical scheme of the present invention is, also comprises and inoculates fermented strains into Xinhui citrus reticulata juice, mulberry juice, Fructus Mali pumilae
Any one or several fruit and vegetable juice in juice, Fructus Citri Limoniae juice, Dragonfruit Juice, Succus Rhizoma Dioscoreae, Pulp Citrulli juice, Fructus Lycopersici esculenti ferments 3
The step of the moon, i.e. can obtain after having fermented containing sour molten albumen, aminoacid, the natural ferment of little peptide.
Scheme two:
A kind of room temperature kills the method for aerobic bacteria, it is characterised in that always held at tank body by content for liquid subject to sterilization
Long-pending 96%, aerobic flora emerges and is conducive to concentrating elimination at liquid surface and middle and upper part, uses any one mode following to go out
Bacterium, A. ethanol does liquid level sterilizing, and aerobic bacteria can be eliminated by the form that ethanol is covered with spraying or liquid level；B uses ozone space
Sterilizing, injects the ozone of the 0.3-0.5% of total volume of air in oxygen-containing space, and the aerobic bacteria being active in liquid surface will touch
It is destroyed to ozone and reaches to eliminate the purpose of aerobic bacteria.
Scheme three:
Xinhui citrus reticulata purposes in preparing ferment.
Use the present invention of as above technical scheme, have the advantages that relative to prior art: room temperature sterilizes and do not loses
Live, make full use of Xinhui citrus reticulata meat, be fermented into power high；Solving prior art problem, initiative solves by same means originally
Technical problem；
As long as the 90 days time fermented again after present invention inoculation i.e. can reach, the normal fermentation effect of 720 days can be reached；
Conventional method is high temperature sterilize, and the most all of bacterium of high temperature sterilize is all gone out, and original natural enzyme also goes out, then
Having and invest relatively large exactly, be unfavorable for producing on a large scale, the present invention method by space disinfection sterilizing, only in aerobic space
Input space biocide, ozone, it is possible to effectively kill the aerobic bacteria in surface existence, and remain anaerobe；
Sugar part depends on time and the fermentation period of fermentable, and very little, substrate does not ferment completely, too, increases
Fermentation time, sugar part of the present invention preparation is just right；
Oxygen-containing space, less than the 5% of total measurement (volume), can anaerobic control to be adjusted well.
Accompanying drawing explanation
In order to further illustrate the present invention, it is further illustrated below in conjunction with the accompanying drawings:
Fig. 1 is the production method flow chart of a kind of ferment of the present invention.
Fig. 2 is jar inventive structure schematic diagram；
Fig. 3 is jar inventive structure top view.
Detailed description of the invention
Illustrating embodiments of the invention below in conjunction with the accompanying drawings, embodiment is not construed as limiting the invention:
The production method of a kind of ferment, it is characterised in that comprise the steps of,
Taking fresh Xinhui citrus reticulata meat, squeeze the juice, load in fermentation tank, loading amount is the 96% of total measurement (volume), sealing, injects sky in oxygen-containing space
The ozone of the 0.3-0.5% of gas cumulative volume, the aerobic bacteria being active in liquid surface will touch ozone and be destroyed and reach to disappear
Go out the purpose of aerobic bacteria；
Standing for fermentation 24 months, terminates fermentation when pH value point is 2.6-3.6；Flora the most therein includes at least cheese milk bar
One or more in bacterium, the secondary dry bacterium of cheese milk, Lactobacillus brevis, acetobacter, are isolated as fermented bacterium by flora standby
With.
The technique effect of the essence that the technical scheme at this place is played and the process that realizes thereof are for one of the most any: A. grinds
Study carefully discovery ferment fermentation tank content when the 96% of tank body total measurement (volume), aerobic flora will substantial amounts of emerge at liquid surface,
Being conducive to concentrating elimination, there is the space of a relative anaerobic in the oxygen-containing space of volume after eliminating aerobic bacteria less than 5% holding, when oxygen-containing
Space can maintain the conditions of existence of aerobic flora more than the amount of oxygen in 5% time space, is unfavorable for the safety in production of product, and little
Oxygen content in 4% time space be not enough to itch flora growth be conducive to the breeding of anaerobe.Simultaneously can be farthest sharp
With container, play greatest benefit;B. in contrast and prior art, " locality either grower or buyer, be intended to skin not
Wanting meat, so may result in Citrus chachiensis Hort. meat not by careless disposal, and can shine into pollution ", prior art can utilize Citrus chachiensis Hort. meat in a large number,
The most therefrom extract and useful ferment.
As the most preferably, before loading in fermentation tank, also comprise the step adjusting pol, the step of described tune pol
Refer to, add sucrose 7-5% when measuring Xinhui citrus reticulata juice pol and being 6-9%, be configured to the material that total pol is 11-13%.
This patent particularly provides system sweat and the control condition of Xinhui citrus reticulata ferment, the composition of fermented product and making
By method, provide a safely and effectively ferment product for vast consumer.The mechanism of the present invention is to utilize idiopathic bacterium
System, test shows: have the fungus strain of varying number, fresh Xinhui citrus reticulata juice just to contain in all of fruit and vegerable: fruit of fermenting on a large scale
The control method of vegetables juice provides a kind of easily operated making approach, it is adaptable to the large batch of deep processing of agricultural product.
The present invention is exactly to search out one safely and effectively room temperature sterilizing methods in test many times, uses the method system
The Xinhui citrus reticulata ferment stock solution made, does not detects harmful bacteria.
Technological process: fruit and vegerable roguing removing the necrotic tissue, goes pesticide residues, drip washing, dehydration, filtration of squeezing the juice, tinning, degassing, sterilizing, joins
Sugar, inoculation, fermentation, process control, ph tests, and terminates fermentation, and classification is sieved degerming.Can make according to existing technique and various want
Product such as: tablet, capsule, granule.Ferment stock solution, the product such as beverage.
More particularly: take fresh Xinhui citrus reticulata meat, squeeze the juice, loading in fermentation tank, loading amount is the 96% of total measurement (volume), joins sugar,
Measuring Xinhui citrus reticulata juice pol is that 6-9% adds sucrose 7-5%, and being configured to total pol is 11-13%, sealing, and ozone inputs
0.3%, (to expect that ullage space calculates) go out aerobic flora, standing for fermentation 24 months, and measuring ph value is 2.6-3.3's
Enzyme liquid carries out microbial count and coliform detection: testing result is that microbe colony is total: 1.76cfu/ml ×
106, coliform is all negative.By antibacterial 16srdna method for identifying molecules, microorganism Pseudomonas in fermentation liquid is identified,
Qualification result shows, containing lactobacillus casei in product, and Lactobacillus paracasei, Lactobacillus brevis.Without miscellaneous bacteria.Terminate the pH value of fermentation
Point is 2.6-3.6；Now measure the content of product: sour molten albumen: 44.6%, 17 kinds of total amino acid contents: 41.2%, little peptide
31%；(reporting attached) cultivates by same procedure after being separated by this flora, as fermented bacterium, inoculates activation in first 10 days
Rear use.
The removal of aerobic bacteria: research finds: when after fruit juice tinning, aerobic bacteria can be tried to be the first at liquid surface.In the hope of existence,
There are two kinds of methods can go out aerobic bacteria.One is to use ethanol to do liquid level sterilizing, the form that ethanol is covered with spraying or liquid level
And aerobic bacteria can be eliminated.But amount is difficult to control, and has lacked DeGrain, many injure anaerobe, shine into fermentation slow.Two
It is with the sterilizing of ozone space.The aerobic bacteria being active in liquid surface when the ozone of 0.3-0.5% is injected in oxygen-containing space will touch
It is destroyed to ozone, thus reaches to eliminate the purpose of aerobic bacteria.
Acidity Control is in the essential condition that pH value is fermented product safe storage between 2.6-3.6, and general microorganism is secondary
Cannot survive in environment, human body is had help human consumption to help the effect that intestinal health is kept ecological balance by lactic acid simultaneously.
Total amount containing ferment (enzyme) is most, so being now off the best period of ferment simultaneously；
When fermentation completes, can effectively terminate fermenting by the vigor of room temperature sterilizing reservation enzyme simultaneously, make the unification that product holding is coherent
Property.
Inventive principle: want carrying out sterilizing without (volume is: 10 cubic metres) in the fermentation tank of stirring system in big production
Being nearly impossible, to power, I inspires the initiative oxygen complementation device from super large fish jar of this patent, and utilize to fish jar oxygenating is former
Reason, by cold antibacterial: in " dimethyl and carbonate " input tank body, can achieve the goal.Checking finds, when " dimethyl and carbon
Hydrochlorate " input.During 0.1%/cubic meter, sampling in 24 hours, microbial check meets state food standard-required.
As the most preferably, strain separating the most also comprising the step of amplification culture, described amplification culture is
Referring to, take fresh Xinhui citrus reticulata meat, squeeze the juice, loading in fermentation tank, loading amount is the 96% of total measurement (volume), stays 4% space；
Add sucrose 7-5% when measuring Xinhui citrus reticulata juice pol and being 6-9%, be configured to the material that total pol is 11-13%；
Input accounts for the ozone of material ullage space 0.3%, knocks out aerobic flora；Remove miscellaneous bacteria；
Then the flora separated, sealing is inoculated by total amount 1-10% of fermentation liquid；
Standing for fermentation 3 months at normal temperatures；
Terminate fermentation when pH value point is 2.6-3.6, stop fermentation in time.
As the most preferably, described prevention fermentation refers to input in tank body by cold antibacterial dimethyl two carbonate,
I.e. can reach keep-alive and terminate the effect of fermentation；Input concentration is the 0.1-0.2% according to the total liquid quality of tank interior.
This place provides amplification culture and the effect of fermentation further.
The further technical scheme of the present invention is, also comprises the step inducing fermented bacterium, and described induction is
Refer to, after using ultraviolet and nitrosoguanidine complex mutation, it is thus achieved that sour molten albumen (enzyme) Producing Strain group of hill.Lead to Xinhui citrus reticulata pure juice
Crossing 720 days normal temperature anaerobic fermentations and obtain the flora can still in relatively highly acidity with stronger vigor, this strain Central China agricultural university leads to
Cross antibacterial 16SrDNA Molecular Identification: for floras such as lactobacillus casei, the secondary dry bacterium of cheese milk, Lactobacillus brevis, acetobacters, use
After ultraviolet and nitrosoguanidine complex mutation, it is thus achieved that sour molten albumen (enzyme) Producing Strain group of hill, connect with 1-5% with this flora
Planting and ferment 3 months in Xinhui citrus reticulata juice, through Central China agricultural university, detection shows: containing sour molten albumen (ferment) total amount: 44.62% analyzes choosing
Select the part enzyme total activity 1519u/g recorded.This strain has stronger vigor and higher at pH value in the environment of 2.6-3.5
Enzymatic productivity, stable performance in industrialized production.Than setting out, the bacterium former flora of Xinhui citrus reticulata juice produces enzyme activity: 11.4574u/g improves
1508u/g.Through repeatedly inducing, find that this induction has definitiveness, use this kind of method that the flora of high yield can occur.
Fresh Xinhui citrus reticulata meat process also comprise roguing removing the necrotic tissue, go pesticide residues, drip washing, be dehydrated, squeeze the juice filter step.
As implementation example, also comprise and inoculate fermented strains into Xinhui citrus reticulata juice, mulberry juice, Sucus Mali pumilae, Fructus Citri Limoniae juice, HUOLONG
The step fermented 3 months in any one or several fruit and vegetable juice in fruit juice, Succus Rhizoma Dioscoreae, Pulp Citrulli juice, Fructus Lycopersici esculenti, has fermented
I.e. can obtain after one-tenth containing sour molten albumen, aminoacid, the natural ferment of little peptide.
Standing for fermentation 24 months, then terminate fermentation, classification is sieved degerming, terminates fermentation and can carry out at any time.Within 24 months, it is about
2 years, overall natural law can somewhat fluctuate.
The present invention, by fruit and vegerable roguing removing the necrotic tissue, goes pesticide residues, drip washing, dehydration, filtration of squeezing the juice, tinning, degassing, sterilizing, joins
Sugar, inoculation, fermentation, process control, ph tests, and terminates fermentation, and classification is sieved degerming.Can make according to existing technique and various want
Product such as: tablet, capsule, granule.Ferment stock solution, the product such as beverage.In test many times, search out a kind of safety have
The room temperature sterilizing methods of effect, the Xinhui citrus reticulata ferment stock solution made by the method, do not detect harmful bacteria.
Acidity Control is in the essential condition that pH value is fermented product safe storage between 2.6-3.6, and general microorganism is secondary
Cannot survive in environment, human body is had help human consumption to help the effect that intestinal health is kept ecological balance by lactic acid simultaneously.
Total amount containing ferment (enzyme) is most, so being now off the best period of ferment simultaneously；
When fermentation completes, can effectively terminate fermenting by the vigor of room temperature sterilizing reservation enzyme simultaneously, make the unification that product holding is coherent
Property.
As long as the 90 days time fermented again after present invention inoculation i.e. can reach, the normal fermentation effect of 720 days can be reached；
Conventional method is high temperature sterilize, and the most all of bacterium of high temperature sterilize is all gone out, and original natural enzyme also goes out, then
Having and invest relatively large exactly, be unfavorable for producing on a large scale, the present invention method by space disinfection sterilizing, only in aerobic space
Input space biocide, ozone, it is possible to effectively kill the aerobic bacteria in surface existence, and remain anaerobe；
Sugar part depends on time and the fermentation period of fermentable, and very little, substrate does not ferment completely, too, increases
Fermentation time, sugar part of the present invention preparation is just right；
Oxygen-containing space, less than the 5% of total measurement (volume), can anaerobic control to be adjusted very well；
Conventional termination fermentation, inhibitor to be added or before preparation is canned, high temperature sterilize processes, and the present invention accomplish at any time with
Ground terminates fermentation.
Scheme two:
A kind of room temperature kills the method for aerobic bacteria, it is characterised in that always held at tank body by content for liquid subject to sterilization
Long-pending 96%, aerobic flora emerges and is conducive to concentrating elimination at liquid surface and middle and upper part, uses any one mode following to go out
Bacterium, A. ethanol does liquid level sterilizing, and aerobic bacteria can be eliminated by the form that ethanol is covered with spraying or liquid level；B uses ozone space
Sterilizing, injects the ozone of the 0.3-0.5% of total volume of air in oxygen-containing space, and the aerobic bacteria being active in liquid surface will touch
It is destroyed to ozone and reaches to eliminate the purpose of aerobic bacteria.
Scheme three:
Xinhui citrus reticulata purposes in preparing ferment.
As a example by Xinhui citrus reticulata ferment:
Cultivating the big beneficial flora of yield of enzyme at fermented product itself, this flora is born between 2.6-3.6 at pH value and is had vigor.
In a creative way, each effect above is individually present, moreover it is possible to complete the combination of the above results with a nested structure.In figure not
Part details is shown.
As preferably:
The step of described elimination aerobic bacteria completes in tank body, and described tank body comprises the oral area of more than four, and a mouth is installed
Relief valve, this relief valve is the check valve of aerofluxus outwardly；One mouth is provided with the Pressure gauge that check that pressure inside the tank；One mouth
PH value sensor port is installed；One mouth is charging aperture；The ozone of the 0.3-0.5% injecting total volume of air in oxygen-containing space i.e. exists
Adding the ozone of the 0.3-0.5% injecting total volume of air in the tank body of material, anaerobe is apnea fermentation, so the fermentation phase
Between will not aerogenesis, when Pressure gauge display pressure be zero and no longer stir when, aerobic bacteria has been killed.Described pH value sensor
Connecing and central control system, H-number sensor is able to record that the acidity change of fermentation.Described tank base comprises sewage draining exit.Described tank body
The position of 10-30 centimetre, baseplane of distance comprises discharging opening.
The material of fermentation tank: (1) stainless steel 304,316, the material of specification.(2) fiberglass acid and alkali-resistance meets food and sends out
The material of engineering ferment material standard, will not bring the material of any pollution to fermented product.
1, function composition: body requirement, function setting: top: have big three little mouths: big mouth is for fermenting later stage feed supplement
To close holding positive confinement state, osculum dress air bleeding valve by, early stage, the function of this valve is can only aerofluxus, it is impossible to air inlet, so
Want individual event valve, only export but no import.Osculum installs Pressure gauge, checks that pressure inside the tank, the reason of pressure inspection are can be intuitively at any time
Show, aerobic bacteria kill situation, (anaerobe be apnea fermentation, so during Fa Jiao will not aerogenesis, otherwise aerobic bacteria osculum
Being pH value sensor port, pH value sensor connects and links with computer, can record the acidity change of fermentation, and this mouth can also be used to
Phase sterilizing front and back is used.Therefore, the top of fermentation tank, minimum want more than three osculums.Middle part has a big mouth (manhole) to use
Carrying out safety inspection, tight-closed at ordinary times, flat mouth at the bottom of bottom side is sewage draining exit.Use for deslagging and cleaning waste water.Slightly go up
The mouth in portion is, discharging opening.Reason is that ferment has fermented, and there is fermentation residue fermentation ferment tank top and the bottom, and middle part is clarification
Liquid, therefore discharging opening is placed on the relatively good use in position of 10-30 centimetre, baseplane of distance.
It should be noted that multiple schemes that this patent provides comprise the basic scheme of itself, separate, the most mutually
Restriction, but it can also be mutually combined in the case of not conflicting, and reaches multiple effect and jointly realizes.As simple illustration:
Selection-breeding condition is the most viable bacterium in the highest environment of acidity, and reason is, general antibacterial is 3.6 to be once to give birth in acidity
Depositing, the purpose of this strain of selection-breeding, one is the stability that can keep product, and two is the growth that can suppress other antibacterial.
Can be simultaneously reached the total amount peak of the enzyme that we want.
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described.The technology of this area
Personnel, it should be recognized that the present invention is not restricted to the described embodiments, simply illustrate this described in above-described embodiment and description
Bright principle, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these changes
Both fall within claimed scope with improving.
Claims (9)
1. the production method of a ferment, it is characterised in that comprise the steps of,
Taking fresh Xinhui citrus reticulata meat, squeeze the juice, load in fermentation tank, loading amount is the 96% of total measurement (volume), sealing, injects sky in oxygen-containing space
The ozone of the 0.3-0.5% of gas cumulative volume, the aerobic bacteria being active in liquid surface will touch ozone and be destroyed and reach to disappear
Go out aerobic bacteria purpose preserve ozone time be 24 hours；
Standing for fermentation 24 months, terminates fermentation when pH value point is 2.6-3.6；Flora the most therein includes at least cheese milk bar
One or more in bacterium, the secondary dry bacterium of cheese milk, Lactobacillus brevis, acetobacter, are isolated as fermented bacterium by flora standby
With.
The production method of a kind of ferment the most as claimed in claim 1, it is characterised in that also comprise tune before loading in fermentation tank
The step of pol, the step of described tune pol refers to, adds sucrose 7-5%, preparation when measuring Xinhui citrus reticulata juice pol and being 6-9%
The material becoming total pol to be 11-13%.
The production method of a kind of ferment the most as claimed in claim 1, it is characterised in that strain separating is the most also comprised expansion
The step cultivated, described amplification culture refers to, takes fresh Xinhui citrus reticulata meat, squeezes the juice, and loads in fermentation tank, and loading amount is total measurement (volume)
96%, stay 4% space；
Add sucrose 7-5% when measuring Xinhui citrus reticulata juice pol and being 6-9%, be configured to the material that total pol is 11-13%；
Input accounts for the ozone of material ullage space 0.3%, knocks out aerobic flora；Remove miscellaneous bacteria；
Then the flora separated, sealing is inoculated by total amount 1-10% of fermentation liquid；
Standing for fermentation 3 months at normal temperatures；
Terminate fermentation when pH value point is 2.6-3.6, stop fermentation in time.
The production method of a kind of ferment the most as claimed in claim 3, it is characterised in that described prevention fermentation refers to cold sterilization
In agent dimethyl two carbonate input tank body, i.e. can reach keep-alive and terminate the effect of fermentation；Input concentration is according in tank body
The 0.1-0.2% of the total liquid quality in portion.
The production method of a kind of ferment the most as claimed in claim 1, it is characterised in that also comprise and fermented bacterium is induced
Step, described induction refers to, after using ultraviolet and nitrosoguanidine complex mutation, it is thus achieved that sour molten albumen (enzyme) superior strain
Group.
The production method of a kind of ferment the most as claimed in claim 1, it is characterised in that fresh Xinhui citrus reticulata meat processes and also comprises
Miscellaneous removing the necrotic tissue, go pesticide residues, drip washing, be dehydrated, squeeze the juice filter step.
The production method of a kind of ferment the most as claimed in claim 1, it is characterised in that also comprise and inoculate fermented strains into newly
Can Citrus chachiensis Hort. juice, in mulberry juice, Sucus Mali pumilae, Fructus Citri Limoniae juice, Dragonfruit Juice, Succus Rhizoma Dioscoreae, Pulp Citrulli juice, Fructus Lycopersici esculenti any one or several
Fruit and vegetable juice in ferment the step of 3 months, i.e. can obtain after having fermented containing sour molten albumen, aminoacid, the natural ferment of little peptide
Element.
8. the method that a room temperature kills aerobic bacteria, it is characterised in that content is total at tank body for liquid subject to sterilization
The 96% of volume, aerobic flora emerges and is conducive to concentrating elimination at liquid surface and middle and upper part, uses any one mode following
Sterilizing, A. ethanol does liquid level sterilizing, and aerobic bacteria can be eliminated by the form that ethanol is covered with spraying or liquid level；B is empty with ozone
Between sterilizing, inject the ozone of 0.3-0.5% of total volume of air in oxygen-containing space, the aerobic bacteria being active in liquid surface will touch
And be destroyed to ozone and reach to eliminate the purpose of aerobic bacteria.
9. Xinhui citrus reticulata purposes in preparing ferment.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106834015A (en) * | 2017-03-01 | 2017-06-13 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of lemon and citrus reticulata"Chachi” composite fermentation fruit wine and preparation method thereof |
| CN106901339A (en) * | 2017-02-22 | 2017-06-30 | 江门酵敬堂生物科技有限公司 | A kind of production method of dried orange peel mandarin orange ferment |
| CN107041529A (en) * | 2017-05-26 | 2017-08-15 | 江门市昊能生物科技有限公司 | A kind of Xinhui citrus reticulata fruit lactic acid ferment juice and preparation method thereof |
| CN111165810A (en) * | 2020-03-12 | 2020-05-19 | 孙培雄 | Production method of ferment and fermentation device thereof |
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| CN106901339A (en) * | 2017-02-22 | 2017-06-30 | 江门酵敬堂生物科技有限公司 | A kind of production method of dried orange peel mandarin orange ferment |
| CN106834015A (en) * | 2017-03-01 | 2017-06-13 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of lemon and citrus reticulata"Chachi” composite fermentation fruit wine and preparation method thereof |
| CN106834015B (en) * | 2017-03-01 | 2021-02-26 | 广东省农业科学院蚕业与农产品加工研究所 | Lemon and tea branch citrus compound fermented fruit wine and preparation method thereof |
| CN107041529A (en) * | 2017-05-26 | 2017-08-15 | 江门市昊能生物科技有限公司 | A kind of Xinhui citrus reticulata fruit lactic acid ferment juice and preparation method thereof |
| CN111165810A (en) * | 2020-03-12 | 2020-05-19 | 孙培雄 | Production method of ferment and fermentation device thereof |
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Application publication date: 20161109 |