CN2923716Y - Automatic cell culturing system - Google Patents
Automatic cell culturing system Download PDFInfo
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- CN2923716Y CN2923716Y CN 200520043615 CN200520043615U CN2923716Y CN 2923716 Y CN2923716 Y CN 2923716Y CN 200520043615 CN200520043615 CN 200520043615 CN 200520043615 U CN200520043615 U CN 200520043615U CN 2923716 Y CN2923716 Y CN 2923716Y
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Abstract
The utility model relates to a biological cells culture device, especially relates to an automatic biological cells auto-culture device. Specifically speaking, the utility model is a biological cell culture system which is characterized in that the type is whole closed, the perfusion is automatic sustainable, the nutrition media and metabolites needed by culture are changed automatically, and culture cells are harvested automatically. The system can be used largely in the field of tumor biotherapy, tumor immunotherapy, hematopoietic stem cells transplantation and adoptive immune cells treatment, etc.
Description
Affiliated technical field
The utility model relates to a kind of biomass cells culture apparatus, relate in particular to the automatic culture apparatus of a kind of automated biological cell, more particularly, this is a kind of Totally-enclosed-type, automatic continuous perfusion, changes and cultivate required nutrition media and meta-bolites, gather in the crops the biomass cells culture systems of culturing cell automatically automatically, and this system can be widely used in fields such as tumor biotherapy, immunotherapy of tumors, hematopoietic stem cell transplantation and adoptive immunity cell therapy.
Background technology
Biological cell therapeutical has been acknowledged as one of the most promising clinical therapeutic modality, a lot of dissimilar biological cell therapeuticals have been widely used in the clinical treatment field, dendritic cell vaccination treatment and tumor vaccine treatment have shown good and clinical curative effect and powerful therapeutic action, and hematopoietic stem cell transplantation has more become the first-selected methods of treatment of neoplastic hematologic disorder.The adoptive immunity cell therapy also has been applied to broad variety oncotherapy and immunotherapy field.
Biomass cells external treatment and amplification are the basis and the important composition parts of biological cell therapeutical, only finish the amplification in vitro of biomass cells, and biological cell therapeutical just might become a reality.The biomass cells training method of present clinical employing is continued to use the mode of biology laboratory more, most adopts open structure, manual operations, so the pollution rate height, efficient is poor, the workload system is heavy, goes wrong easily, influences carrying out in a deep going way of biomass cells.
Summary of the invention
For the defective that overcomes prior art with remedy the deficiencies in the prior art, the purpose of this utility model provides a kind of new type auto cell culture system, reduce in the culturing process and pollute incidence, increase the efficient of cell cultures, reduce the workload of personnel in the culturing process.Feature of the present utility model is:
● the system lock of creation, firm is fit to clinical aseptic technique requirement.
● automatic continuous perfusion functional requires timing automatic to add somatomedin or nutritive medium according to cell cultures.
● under totally-enclosed environment and pollution-free prerequisite, change nutrient solution.
● harvested cell under totally-enclosed environment and pollution-free prerequisite.
● nutrient solution that automatic continuous eliminating has exhausted and products of cellular metabolism.
● the semi-permeable membranes of employing is effectively finished the gaseous interchange function.
● the adherent cell plane is arranged, be fit to the adherent cell growth.
● micro processor control repertoire.
● the touch screen computer screen makes simple, convenient.
● perfect computer record function makes the cell cultures data complete comprehensively.
● can require and client's needs and design coventional type, semi-automatic, automatic type respectively according to difference.
● designed system can satisfy different levels, dissimilar clinical biological cell therapeutical.Include but not limited to:
Treatment of the external auto culturing system of ■ dendritic cell-be applied to dendritic cell vaccination and tumor vaccine treatment.
The external auto culturing system of ■ hemopoietic stem cell-be applied to bone marrow transplantation, autologous peripheral blood stemcell transplant and navel blood stem cell transplant.
The external auto culturing system of ■ lymphocyte-LAK cell, til cell, adoptive immunity cell therapies such as CTL cell, CAK cell.
The utility model constitutes:
1. chamber (disposable use bio-reactor) is cultivated in disposable use
● treated adhesion material is all adopted on four planes in the bio-reactor, promptly requires semi-permeable membranes to adopt hydrophilic surface (Hydrophilic Surface) so that increase culturing cell density.
● four manually-operated gate ends all install cap additional, and its end all should have standard interface be connected with silicone tube (pipe connecting that is peristaltic pump).It is fixing to add block at ordinary times, when installing pipeline additional, needs to operate under gnotobasis at every turn, or needs to operate under ultraviolet lamp, or all need with cotton ball soaked in alcohol sterilization to clean before changing pipeline at every turn.
● on original framework, install the plastics crossbeam of three vertical direction additional,, also be convenient to fix to reinforce the ultimate compression strength of bio-reactor, because install additional at the crossbeam place pickup groove make bio-reactor easily and support fix, and be convenient in the special-purpose drawer of seasoning in CO2gas incubator.
● four manually-operated gates and connecting tube are installed plastic stent, and these supports are used for fixing supports four manually-operated gates and pipeline, and can combine closely with plastic frame, helps connecting external pipeline.
2. the utility model is supported total system by two big supports
1) bio-reactor support
● the top is three infusion supports, is used for hanging inoculating cell bag (No. 1 bag), nutritive medium bag (No. 2 bags), damping fluid bag (No. 3 bags).
● the bio-reactor riser, support three infusion supports, and fix 1., 2., 3., 4., 5., 6., 7. number magnetic valve is fixed " cytokine " test kit, " biotic factor " test kit and No. 1 peristaltic pump simultaneously.
● the bio-reactor transverse slat, the fixed biologically reactor, framework and centre cross member around utilizing are fixed four manually-operated gates, fix four manually-operated gates and link to each other with corresponding pipeline, the fluted reactor of fixed biologically steadily on the transverse slat.The electromagnetic oscillation agitator is equipped with in the transverse slat bottom that is equivalent to the bio-reactor position, is used for liquid in the stirring and evenly mixing bio-reactor.
● on the transverse slat surface, control and display panel are arranged, and power supply, automatic control device are fixed on all in the transverse slat, control panel has a plurality of membrane keyboard switch controls, and each is partly relevant, and big liquid crystal display can show related data, (control and demonstration partly can be controlled by touch screen computer).
2) TFF system frame.Parallel with the bio-reactor support, put the consecutive position, be used for fixing No. 1 and No. 2 peristaltic pumps, 8., 9., 10., , , , magnetic valve.The TFF pan straddle is upright to being fixed on the support, is used for supporting the TFF container.There are two feet supporting brackets TFF system frame below.
3) Liquid storage bag
● No. 1 bag-storage inoculating cell liquid
● No. 2 bag-storage nutrient mediums
● No. 3 bag-storage buffer
● the liquid bag is abandoned in No. 6 bag-storages
4) No. 4 boxes are " cytokine " test kit and " somatomedin " test kit, and No. 4 boxes are used for injecting or continue and pour into various cytokines, somatomedin or other micro substances, can be used for continuing perfusion or gap and pour into or regularly pour into.
5) No. 5 boxes are for continuing perfusion nutrient medium box, are used for continuing the necessary nutritive medium of perfusion (include the various cells necessary nutrition of growing, and all kinds of essential somatomedin, cytokine etc.).
6) incubation chamber of contain dry freeze is used for storing, fixing No. 4 boxes and No. 5 boxes, guarantee to continue dabbling nutritive medium or (with) cytokine, somatomedin be in freezing state.Incubation chamber is in sealed state, built-in dry ice or dry ice bag, or refrigerating fulid bag, be seated in No. 4 and No. 5 boxes around, can keep a couple of days freezing state, in case of necessity, can replace wherein dry ice, can built-in thermometer, reach a certain state in temperature and report to the police automatically.No. 4, two holes and No. 5 holes are arranged in the incubation chamber, and pipeline stretches out, and should seal around the outlet opening, prevents that temperature from sending out.
7) disposable bioreactor, be seated in bio-reactor support transverse slat, can fix or remove, there is stationary installation to be attached thereto, fluted on the transverse slat, can directly put bio-reactor, the fixer that the pair of parallel y direction is arranged in the transverse slat both sides, can be used for fixing the movable fixing strip of perpendicular direction, fixing strip can be made of elastomeric material, has nylon zipper or buckle is set up fixed action, duroplasts crossbeam direction on movable fixing strip and the disposable bioreactor, shape, the position is consistent, be mainly used to steadily fixedly disposable bioreactor, make it to be difficult for moving, activity, but can not oppress bio-reactor.
8) control panel is positioned at the transverse slat lower left of bio-reactor support, by control partly and show partly and form.
● control is partly.Adopt the membrane keyboard on-off control, by DSP (Digital Signal Processor) or (microcontroller) control all operations, function.Comprise:
A. power supply open and close.
B. peristaltic pump open and close, speed adjustment.
C. solenoid control open and close.
● show partly.Adopt big screen LCD to show each position function.
A. power supply open and close state.
B. peristaltic pump working order, rotating speed.
C. valve opening solenoid off status (magnetic valve distribution plan, working order indication etc.).
9) peristaltic pump, No. 1 peristaltic pump is installed in bio-reactor support riser, No. 2 peristaltic pump is installed in the TFF system frame, adopt Digital Control and digitizing to regulate, the digitizing display type, peristaltic pump adopts special silicone tube, and peristaltic pump speed can electrodelessly be regulated, and can digitizing display working condition and rotating speed.
10) electromagnetism squeeze valve, all valves all adopt the electromagnetism squeeze valve, control its on off state by DSP.And on liquid crystal display, show the overall work state.Magnetic valve can be controlled by manual switch, also can be by presetting the control of DSP internal program.
11) tubing system, all connecting tubes are nontoxic, harmless, no absorptivity, and the silicone tube that biocompatibility is good, pipe joint adopt nontoxic element duroplasts.Strict sterilization before pipeline uses, interface disinfects in alcohol before connecting equally.Pipeline can be reused behind the cleaning and sterilizing, can adopt disinfection with high pressure steam method or ultraviolet sterilization.
12) TFF box adopts the opening structure ultra-filtration membrane (UF) that is exclusively used in cell harvesting, aperture 0.2 μ m, and sterilization in advance before the use, entrance and exit links to each other with pipeline, disinfects in alcohol before the connection.Can be fixed on the TFF support, or be fixed on the TFF container, through cleaning, reusable behind the autoclave sterilization.
13) TFF container adopts standard TFF container, according to cultivation amount difference, selects not isometric TFF container for use.
The beneficial effects of the utility model are, propose a kind of new type auto biomass cells culture systems, and are simple and practical, the demand that is fit to clinical large number of biological cell expansion ex vivo, reduce the sample pollution rate significantly, improve amplification in vitro efficient, promote popularizing and promoting of clinical biological cell therapeutical.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Fig. 1 is a disposable bioreactor profile synoptic diagram
Fig. 2 is the manually-operated gate synoptic diagram.
Fig. 3 is the crossbeam synoptic diagram.
Fig. 4 continues filling type auto culturing system synoptic diagram.
Fig. 5 is the incubation chamber synoptic diagram.
Fig. 6 is the disposable bioreactor stationary installation.
Fig. 7 is the control panel synoptic diagram.
Embodiment
201-block among Fig. 2,202-outside nut interface, 203-manual valve.304-manually-operated gate among Fig. 3,305-plastic frame, 306-crossbeam.401-inoculating cell among Fig. 4,402-nutrient medium, 403-damping fluid, 404-support, 405-dotted line partly for storing the dry freeze incubation chamber, are used for fixing and place 4 ' box and 5 ' box.406-cytokine/somatomedin test kit, 407-continues perfusion culture nutritive medium, 408-peristaltic pump (1), 409,410-bio-reactor support, 411-bio-reactor valve, 412-control panel, 413-electromagnetic oscillation agitator, 414-damping fluid (2), 415-abandons the liquid bag, 416-peristaltic pump (3), 417-peristaltic pump (2), the 418-TFF carriage, the 419-TFF container.501-thermal insulation layer among Fig. 5,502-dry ice, 503-4 ' box and 5 ' box.601-bio-reactor fixer among Fig. 6,602-bio-reactor groove, 603-nylon draw key or metal finger button.704-peristaltic pump speed setting among Fig. 7, the control of 705-electromagnetism squeeze valve, 706-liquid-crystal display, 707-power switch.
Claims (3)
1. automatic cell culture system is characterized in that: automatic cell culture system is by big being configured to of disposable cultivation chamber and two.
2. according to the described automatic cell culture system of claim 1, it is characterized in that: disposable use is cultivated interior four planes of bio-reactor of chamber and is all adopted treated adhesion materials, require to adopt the hydrophilic surface of semi-permeable membranes, four manually-operated gate ends all install cap additional, and its end all should have standard interface to be connected with silicone tube, four manually-operated gates and connecting tube are installed plastic stent, these supports are used for fixing supports four manually-operated gates and pipeline, and can combine closely with plastic frame, help connecting external pipeline.
3. according to the described automatic cell culture system of claim 1, it is characterized in that: support total system by two big supports, bio-reactor support one top is three infusion supports, be used for hanging the inoculating cell bag, the bio-reactor riser supports three infusion supports and Motionless electromagnetic valve, fixedly all ingredients box and peristaltic pump simultaneously, bio-reactor transverse slat fixed biologically reactor, framework and centre cross member around utilizing, fix four manually-operated gates, fixing four manually-operated gates links to each other with corresponding pipeline, the fluted reactor of fixed biologically steadily on the transverse slat, the electromagnetic oscillation agitator is equipped with in the transverse slat bottom that is equivalent to the bio-reactor position, on the transverse slat surface, control and display panel are arranged, and power supply, automatic control device all is fixed in the transverse slat, control panel has a plurality of membrane keyboard switch controls, and each is partly relevant, and big liquid crystal display is used to show related data, TFF system frame one is parallel with the bio-reactor support, and put the consecutive position, is used for fixing peristaltic pump, magnetic valve, the TFF pan straddle is upright to be used for supporting the TFF container to being fixed on the support, and there are two feet supporting brackets TFF system frame below.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520043615 CN2923716Y (en) | 2005-07-21 | 2005-07-21 | Automatic cell culturing system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520043615 CN2923716Y (en) | 2005-07-21 | 2005-07-21 | Automatic cell culturing system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2923716Y true CN2923716Y (en) | 2007-07-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520043615 Expired - Fee Related CN2923716Y (en) | 2005-07-21 | 2005-07-21 | Automatic cell culturing system |
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| Country | Link |
|---|---|
| CN (1) | CN2923716Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103068962A (en) * | 2010-08-12 | 2013-04-24 | 株式会社日立制作所 | Automatic culture device |
| CN105154329A (en) * | 2010-08-12 | 2015-12-16 | 株式会社日立制作所 | Automatic culture apparatus |
-
2005
- 2005-07-21 CN CN 200520043615 patent/CN2923716Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103068962A (en) * | 2010-08-12 | 2013-04-24 | 株式会社日立制作所 | Automatic culture device |
| CN103068962B (en) * | 2010-08-12 | 2015-08-05 | 株式会社日立制作所 | Automatic culture apparatus |
| CN105154329A (en) * | 2010-08-12 | 2015-12-16 | 株式会社日立制作所 | Automatic culture apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |