CN201485469U - Loading device for double frequency loading of cultures - Google Patents
Loading device for double frequency loading of cultures Download PDFInfo
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- CN201485469U CN201485469U CN2009200985493U CN200920098549U CN201485469U CN 201485469 U CN201485469 U CN 201485469U CN 2009200985493 U CN2009200985493 U CN 2009200985493U CN 200920098549 U CN200920098549 U CN 200920098549U CN 201485469 U CN201485469 U CN 201485469U
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Abstract
The utility model discloses a loading device for double frequency loading of cultures, comprising a cultivation cell and a loading mechanism for conducting double frequency loading on the cultures in the cultivation cell, wherein the double frequency loading mechanism comprises a support seat, an upright post arranged on the support seat, a top plate supported by the upright post and a cultivation cell arranged between the top plate and the support seat, a worktable which can move up and down is arranged in the middle of the support seat, a vibration exciter is fixed on the worktable, a cultivation table is fixed on the vibration exciter, and the cultivation cell is installed on the cultivation table; a lower pressing block is arranged at the bottom part of the cultivation cell, an upper pressing block which can move up and down is installed at the top part of the cultivation cell, the lower end of the upper pressing block extends into the cultivation cell, and the cultures are arranged between the upper pressing block and the lower pressing block; and a pilot sleeve is installed on the top plate, piezoceramics are fixed on the upper pressing block by the guide sleeve, and pre-tightening adjusting screws are screwed in from the upper end of the guide sleeve so that the piezoceramics, the upper pressing block, the cultures and the lower pressing block can contact sequentially.
Description
Technical field
The utility model relates to the bio-reactor field, particularly relates to a kind of deceleration loading device that culture is carried out the double frequency loading.
Background technology
After Celluar and Molecular Biology, a new milestone---organizational project has appearred again on the life science development history.This indicates that medical science will not only rely on organ transplantation, but has entered the New Times of making tissue and organ.
In clinical medicine, usually run into because the articular cartilage defect that chronic diseases such as wound, excision, osteoarthritis cause.A large amount of research experiments show that because the regenerative agent of cartilaginous tissue ability is very limited, the cartilage injury self-regeneration almost is impossible.And autotransplantation or heteroplastic transplantation all exist bigger limitation and drawback, and for example material chooses or rejection etc.Under this background, cartilage tissue engineeredly become a kind of comparatively ideal method.Cartilage tissue engineered basic skills is a similar environment in one of external achievements and body, will be after vitro culture, amplification from body or variant cell, be inoculated on the good timbering material of a kind of histocompatibility and carry out vitro culture, implant the health defect then, merge with host's cartilage, reach the purpose of repairing cartilage defect.Using the purpose of bio-reactor is for external cartilage tissue engineered structure provides suitable growing condition, makes engineering tissue obtain structure and the function similar to biological tissue.
The current bio-reactor that is used for the osseous tissue cultivation mainly contains three kinds: rotating and culturing bottle, microgravity rotating biological reactor and perfused tissue culture systems.Different seed cells, New-support material, somatomedin etc. constitute culture, in these several bio-reactors, cultivate respectively, and mainly be the growth that promotes culture form, structure.
Although the improvement of various bio-reactors makes the growth of culture obtain to a certain degree optimization, the clinical requirement but the growth of culture, growth still fail to reach, as: when engineering tissue was cultivated in bio-reactor, problems such as artificial organ dysplasia, poor mechanical property, cell degradation, death had appearred.Address these problems and need be optimized the bioreactor culture condition, make engineering tissue can external obtain with body in similar or identical growing environment.Wherein mechanical environment occupies critical positions in the development of bio-reactor, and the optimization of mechanical condition is very important.
Along with the development of organizational project, people are to the biomechanics of graft and culture culture condition and going deep into of mechanics biology understanding, and recent years, the investigator invented different mechanical stimulations, to optimize the 26S Proteasome Structure and Function of culture.As: the investigator adopts quiet tension stress that the rat condylar cell propagation effect is studied, and experiment shows that cell proliferation quantity increases with quiet tension stress and raise [West China stomatology magazine, 2003,21 (1), 57-60]; Centrifugal force [the Chinese experimental surgery magazine that in the external structure tissue engineering bone/cartilage, plays a role.2005，22(3)，281-283]。These loading methods can promote the performance of cell proliferation or function, but and real cartilage stand under load be differentiated.Mauck etc. have studied the variation of different incubation time culture Young's modulus under mechanical condition.International Application No. WO 2004/046304 has provided the bio-reactor of pressure and shearing stress coupling; Schulz and Bader, the environment when Ivan Martin has summed up tissue culture [TRENDS in Biotechnology Vol.22No.2,2004, pp80-86; Eur Biophys J, DOI 10.1007/s00249-007-0139-1,2007], comprise (1) electric field; (2) cone viscosimeter condition, cell is subjected to shear action below the rotary conic body; (3) rotating wall vessel bioreactor provides microgravity environment to cell, and mass transfer process is better; (4) individual event perfusion system; (5) stretching condition; (6) hydraulic pressure and perfusion condition; (7) directly compression, and introduced various culture condition coupled system, particularly various mechanical conditions unite use.
But above-mentioned research does not all relate to the cultivation that low frequency high-amplitude overlapped high-frequency loads by a narrow margin, and in fact, the condition of chondrosin dis environment is necessary in this simulation human body.People's joint cartilaginous tissue when walking is subjected to the effect of complex load, the load of different frequency is not quite similar to the mechanics influence and the living environment of joint cartilage, therefore the acting in conjunction by a narrow margin of low frequency high-amplitude overlapped high-frequency should be the mechanical environment of suitable joint cartilage, so the application of double frequency load can be optimized the mechanical condition of bio-reactor.
The utility model content
The purpose of this utility model is in order to overcome the deficiency of existing engineering tissue culture technique, and a kind of deceleration loading device that culture is carried out the double frequency loading that proposes, load by double frequency and to make the growing environment of through engineering approaches cartilage approach the condition of culturing in vivo more, so as to turn out with body in function and structural similitude or identical tissue or organ.
For this reason, the technical solution of the utility model is as follows:
A kind of deceleration loading device that culture is carried out the double frequency loading, comprise that inside is provided with the cultivation cell of culture, also comprise culture is carried out the load maintainer that double frequency loads, described double frequency load maintainer comprises: bearing, be arranged on the column on the bearing, top board by upright supports, be arranged on the cultivation cell between top board and the bearing, be provided with the worktable that can move up and down at the bearing middle part, on worktable, be set with vibrator, on vibrator, be installed with the cultivation platform, cultivating cell is installed on the cultivation platform, be provided with lower lock block in the bottom of cultivating cell, at the top of cultivating cell the upper holder block that can move up and down is installed, the lower end of upper holder block is stretched into and is cultivated in the cell, culture is arranged between upper holder block and the lower lock block, one pilot sleeve is installed on top board, piezoelectric ceramics is fixed on the described upper holder block by pilot sleeve, and the pretension set screw screws in from the pilot sleeve upper end, makes piezoelectric ceramics, upper holder block and culture, lower lock block keeps in touch in loading procedure.
The longitudinal cross-section of described worktable is a T shape, and its underpart and described bearing are by being threaded.Extend upward in the center upper portion of vibrator and to be formed with push rod, be formed with sleeve in the bottom of cultivating platform, the push rod of vibrator inserts in the sleeve of cultivating platform, and is fixed together by holding screw.
Described cultivation cell is formed with cultivation cell top cover by cultivating the cell main body, and cultivating the cell main body is the cylindrical of no end face, its inner formation culture space, and the both sides of cultivation cell main body are respectively equipped with the nutrient solution inlet and metabolite exports.
Described lower lock block is circular, is formed with the groove with T shape longitudinal cross-section at the top cover middle part of described cultivation cell, and described upper holder block is arranged in the groove, and the shape of upper holder block and the shape of groove adapt.
In addition, described deceleration loading device also comprises the computer that described piezoelectric ceramics and vibrator are controlled.
The deceleration loading device that culture is carried out the double frequency loading of the present utility model, simultaneously culture is loaded up and down, by regulating the frequency of load maintainer, culture is applied double frequency load, make and the more approaching intravital true environment of mechanical environment of bone or cartilage vitro culture make the function of cultured tissue and function and the structure that structure more approaches in-vivo tissue.
Description of drawings
Fig. 1 is front schematic view of culture being carried out the deceleration loading device of double frequency loading of the present utility model.
Wherein:
1. top board; 2. pilot sleeve; 3. pretension set screw;
4. column; 5. piezoelectric ceramics; 6. upper holder block;
7. cultivation cell; 7a. cultivate the cell top cover; 7b. cultivate the cell main body;
8. culture; 9. lower lock block; 10. nutrient solution enters the mouth;
12. metabolite outlet; 13. cultivation platform; 14. holding screw;
15. vibrator; 16. bearing; 17. adjustable bed
Embodiment
The method that deceleration loading device of the present utility model uses double frequency to load loads culture.Its structure comprises as shown in Figure 1: double frequency load maintainer and the cultivation cell 7 that is arranged on the double frequency load maintainer, culture 8 are placed on to be cultivated in the cell 7, by the double frequency load maintainer culture 8 is carried out double frequency and loads.
Specifically, be provided with the worktable 17 that can move up and down at bearing 16 middle parts, the longitudinal cross-section of worktable 17 is a T shape, and its underpart and bearing 16 are connected by screw thread, are used for regulating the displacement of vertical direction and directly bear load.Worktable 17 is provided with vibrator 15, is connected by bolt between worktable 17 and the vibrator 15, helps vibrator like this and works reposefully.Be provided with cultivation platform 13 on the top of vibrator 15, vibrator 15 is to realize by the push rod of vibrator 15 is fixed together with holding screw 14 with the sleeve of cultivating platform 13 with cultivating platform 13, like this, can keep being synchronized with the movement between vibrator 15 and the cultivation platform 13, finish the transmission of vibrator 15 frequencies and amplitude jointly.Cultivate cell 7 and be placed on cultivation platform 13, by cultivating cell main body 7b and cultivating cell top cover 7a and form, cultivating cell main body 7b is the cylindrical of no end face, its inner culture space that forms.The both sides of cultivating cell main body 7b are respectively equipped with nutrient solution inlet 10 and metabolite outlet 12, and being carried in the cultivation cell 7 of the cultivation of culture 8 and double frequency load finished.The bottom center of cultivating cell main body 7b fixedly installs and is useful on the lower lock block 9 that supports culture 8, and lower lock block 9 is preferably circle; Cultivating the groove that is formed with at cell top cover 7a middle part with T shape longitudinal cross-section, in groove, be provided with upper holder block 6, the shape of upper holder block 6 and the shape of groove adapt and can move at above-below direction, the lower end of upper holder block 6 is stretched into and is cultivated in the cell 7, is provided with culture 8 between upper holder block 6 and lower lock block 9.
In addition, top board 1 is provided with a pilot sleeve 2, connects by screw thread between the two.Piezoelectric ceramics 5 is fixed on the top of cultivating cell top cover 7a by pilot sleeve 2, and pretension set screw 3 screws in the pilot sleeve 2 from the upper end.Can guarantee that by regulating pretension set screw 3 piezoelectric ceramics 5, T shape upper holder block 6 and culture 8, lower lock block 9 keep excellent contact in loading procedure.
Cultivating cell 7 is containers of a sealing, possesses conditions such as culture growth required suitable CO2, O2 and temperature, guarantees the supply of somatomedin, nutritive substance etc. in the culturing room and the timely eliminating of culture metabolism product.
The use and the loading method of deceleration loading device of the present utility model are as follows:
At first to the top cover 7a of culturing room, cultivate cell main body 7b, upper holder block 6, lower lock block 9 and carry out disinfection, under aseptic condition, culture 8 is inoculated into then on the lower lock block 9 in the culturing room 7.Elder generation is transferred to the vertical distance of worktable 17 minimum, then the push rod of cultivating platform 13 and vibrator 15 is fixed together, and installs successively and cultivates cell 7 and piezoelectric ceramics 5.The height of regulating worktable 17 and pretension set screw 3 is at last regulated the preliminary tension between the each several part, applies preload to artificial organ.
Then, give piezoelectric ceramics 5 and vibrator 15 input electrical signals under the control of computer, the i.e. acting in conjunction under action of electric signals of piezoelectric ceramics 5 and vibrator 15 applies double frequency load to culture, thereby realizes the loading to artificial organ.Loading frequency and amplitude can be regulated by computer, and the frequency of the two can be identical with amplitude, also can be different.For example, the range of frequency of piezoelectric ceramics 5 can be 0-100Hz, and range of strain is 0.000001-0.01, and the high-frequency range of vibrator 15 can be to be 30～60Hz, and range of strain can be 0.1～0.3, also can adjust as required.
The deceleration loading device that this double frequency loads is relatively simple for structure, is easy to control, conveniently experimentizes, and more is applicable to simultaneously to study in the laboratory and use.It can be placed in the incubator, also can be independent of outside the incubator, culture is carried out double frequency load.
Claims (6)
1. one kind is carried out the deceleration loading device that double frequency loads to culture, comprise that inside is provided with the cultivation cell of culture, it is characterized in that: also comprise culture is carried out the load maintainer that double frequency loads, described double frequency load maintainer comprises: bearing, be arranged on the column on the bearing, top board by upright supports, be arranged on the cultivation cell between top board and the bearing, be provided with the worktable that can move up and down at the bearing middle part, on worktable, be set with vibrator, on vibrator, be installed with the cultivation platform, cultivating cell is installed on the cultivation platform, be provided with lower lock block in the bottom of cultivating cell, at the top of cultivating cell the upper holder block that can move up and down is installed, the lower end of upper holder block is stretched into and is cultivated in the cell, culture is arranged between upper holder block and the lower lock block, one pilot sleeve is installed on top board, piezoelectric ceramics is fixed on the described upper holder block by pilot sleeve, the pretension set screw screws in from the pilot sleeve upper end, makes piezoelectric ceramics, upper holder block and culture, lower lock block keeps in touch in loading procedure.
2. deceleration loading device according to claim 1 is characterized in that: the longitudinal cross-section of described worktable is a T shape, and its underpart and described bearing are by being threaded.
3. deceleration loading device according to claim 1, it is characterized in that: extend upward in the center upper portion of vibrator and be formed with push rod, be formed with sleeve in the bottom of cultivating platform, the push rod of vibrator inserts in the sleeve of cultivating platform, and is fixed together by holding screw.
4. deceleration loading device according to claim 1, it is characterized in that: described cultivation cell is by cultivating the cell main body and cultivating the cell top cover and form, cultivating the cell main body is the cylindrical of no end face, its inner culture space that forms, the both sides of cultivating the cell main body are respectively equipped with nutrient solution inlet and metabolite outlet.
5. deceleration loading device according to claim 4, it is characterized in that: described lower lock block is for circular, top cover middle part at described cultivation cell is formed with the groove with T shape longitudinal cross-section, and described upper holder block is arranged in the groove, and the shape of upper holder block and the shape of groove adapt.
6. according to each described deceleration loading device in the claim 1～5, it is characterized in that: also comprise the computer that described piezoelectric ceramics and vibrator are controlled.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200985493U CN201485469U (en) | 2009-08-31 | 2009-08-31 | Loading device for double frequency loading of cultures |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200985493U CN201485469U (en) | 2009-08-31 | 2009-08-31 | Loading device for double frequency loading of cultures |
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| CN201485469U true CN201485469U (en) | 2010-05-26 |
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| CN2009200985493U Expired - Fee Related CN201485469U (en) | 2009-08-31 | 2009-08-31 | Loading device for double frequency loading of cultures |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559493A (en) * | 2012-02-15 | 2012-07-11 | 四川大学 | Microvibration loading device for bone formation related cells |
| CN106399096A (en) * | 2016-10-17 | 2017-02-15 | 西南交通大学 | Cell culture box |
| CN109642202A (en) * | 2016-06-29 | 2019-04-16 | 通用电气公司 | The method and apparatus of closed system culture for cartilaginous tissue |
-
2009
- 2009-08-31 CN CN2009200985493U patent/CN201485469U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559493A (en) * | 2012-02-15 | 2012-07-11 | 四川大学 | Microvibration loading device for bone formation related cells |
| CN109642202A (en) * | 2016-06-29 | 2019-04-16 | 通用电气公司 | The method and apparatus of closed system culture for cartilaginous tissue |
| CN109642202B (en) * | 2016-06-29 | 2022-09-02 | 环球生命科技咨询美国有限责任公司 | Method and apparatus for closed system culture of cartilage tissue |
| CN106399096A (en) * | 2016-10-17 | 2017-02-15 | 西南交通大学 | Cell culture box |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100526 Termination date: 20120831 |