CN201883096U - Double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor - Google Patents
Double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor Download PDFInfo
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- CN201883096U CN201883096U CN2010205971019U CN201020597101U CN201883096U CN 201883096 U CN201883096 U CN 201883096U CN 2010205971019 U CN2010205971019 U CN 2010205971019U CN 201020597101 U CN201020597101 U CN 201020597101U CN 201883096 U CN201883096 U CN 201883096U
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Abstract
The utility model relates to a double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor which comprises a loading device frame, a motor driving mechanism, a piezoelectric ceramic loading mechanism, a radial twisting mechanism and a culturing room, wherein the loading device frame is formed in a way that an upper base and a lower base are fixedly connected by three stand columns; the motor driving mechanism comprises a motor, a cam bracket, a cam and a cam shaft; the piezoelectric ceramic loading mechanism comprises a sleeve, piezoelectric ceramics, an ejector rod, an insulating board and a loading head; the radial twisting mechanism comprises a stepping motor and a loading platform; the culturing room comprises a culturing room base, a culturing room body and a culturing room cover, a cultured plant is arranged on the culturing groom base, and the loading head applies pressure on the cultured plant. The double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor has the following advantages: through applying two types of loading on the cultured plant, namely, twisting and double-frequency pressurizing, a mechanics environment approaching to the growing and the working conditions of lumbar intervertebraldisc of a human body is created; and the bioreactor is compact in structure, flexible in driving and high in micrometric displacement precision, and has very important application value.
Description
Technical field
The utility model relates to the biomedical engineering technology field, particularly is that a kind of double frequency that is used for external structure functionalization disc tissue compress-radially reverses disc tissue engineering bio-reactor.
Background technology
In the orthopaedics field, because of low back pain and other spondylopathys that intervertebral disc degeneration causes have caused serious economy and emotional distress for people's life.Along with improving constantly of medical level technology, adopted many novel methods, novel material to treat clinically, but still do not had suitable method to obtain satisfactory therapeutic effects.The technology of lot of domestic and international scholar application organizes engineering science and method are set up bone tissue engineer, cartilage tissue engineered etc. in recent years, same disc tissue engineering is as a kind of new technological line, for the morphological structure of regression intervertebral disk and the holomorphosis reparation of physiological function have brought hope.
Disc tissue is made up of nucleus pulposus, fibrous ring and cartilage plate three parts, and it is a kind of viscoelastic material, can absorb concussion or motion is delivered to herein energy, and the motion and the physiological function of backbone had material impact.According to real physiology stand under load research in the intervertebral disk body, we know that the bearing mode of intervertebral disk is very complicated, normally compression, crooked and reverse compound.At present, the cultivation of disc tissue mostly is individual layer and three-dimensional static cultivation, and these traditional static tissue cell culture processes and system are difficult to satisfy structure structure-required requirement of functionalization disc tissue organ knot.Many studies show that kept the mechanics of machinery load that the normal structure of intervertebral disk, composition and mechanics of machinery characteristic all need certain basis amount.
Current, along with the development of organizational project, how deepening continuously of seed cell, timbering material and active factor research is cultivated graft and is subjected to more concern in bio-reactor, wherein the mechanical environment of graft culture condition is more and more paid attention to.Chinese scholars correlative study person's recent years has done big quantity research to different mechanical stimulations with the 26S Proteasome Structure and Function of optimizing culture.Scholar's research is arranged novel rotating wall vessel bioreactor to the structure of tissue engineered bone (biological chemistry and biophysics progress, 2004,31(11), 996-1004); After the amiable enucleation of lumber ertebral disc fatigue loading to the influence of intervertebral disk form (neck pain in the back magazine, 2009,30(4), 306-309); (Spine, 2005,30 (5): 505-511) confirm that the mechanical load difference is different with substrate composed influence to the metabolism of oxtail vertebra nucleus pulposus cell such as Wiseman; J. C. Lotz then studied Static Compression and dynamic compression load to the influence of intervertebral disc degeneration process (Biochemical Society Transactions, 2002,30(6), 853-858).Patent WO2004/046304 has provided the bio-reactor of pressure and shearing stress coupling; Patent of invention (application number 200480038317.9, publication number CN 1898375A) has been introduced and has been used for method and bio-reactor that the Transplanted cells body with 3D shape, activity and mechanical is cultivated and stimulated.Be used for the bio-reactor that applies rolling load and slip load (application number 200720096199, publication number 000000000) that cartilaginous tissue is cultivated, introduced the importance of bionical power load for tissue culture.But at present, disc tissue is not cultivated and carried out double frequency and compress-radially rotate the report of deceleration loading device.
Can whether the mechanical condition of vitro culture with in the body be subjected to force environment consistent really, be in the key of external structure functionalization disc tissue.The utility model, according to the stand under load feature of human intervertebral disc and the influence of organizational project being cultivated in conjunction with mechanics factor, design the deceleration loading device of rotation of axial double frequency compression-radially, this device aims to provide the axial compression of single different amplitudes, different amplitude and the frequency double frequency loads and to the twisting resistance effect that the fibrous ring tissue has vital role, be built be beneficial to tissue growth, growth be similar to the mechanical environment of intervertebral disk at the body state.
Summary of the invention
The purpose of this utility model is according to prior art, at the disease of human intervertebral disc aspect and provide a kind of compact construction, volume is little, driving is sensitive, the micrometric displacement precision is high double frequency compress-radially to reverse disc tissue engineering bio-reactor.
The technical solution of the utility model:
A kind of double frequency compress-radially reverses disc tissue engineering bio-reactor, comprise deceleration loading device framework, motor-driven mechanism, piezoelectric ceramics load maintainer, radially twist mechanism and culturing room, the deceleration loading device framework is fixedlyed connected by three root posts with lower bottom base by upper bed-plate and is constituted; Motor-driven mechanism comprises motor, Cam rest, cam and camshaft, and motor and Cam rest are fixed with upper bed-plate respectively, and cam is arranged on the Cam rest and by camshaft fixedlys connected with motor shaft; The piezoelectric ceramics load maintainer comprises sleeve, piezoelectric ceramics, push rod, insulcrete and loading head, the following of sleeve and upper bed-plate fixed, piezoelectric ceramics is arranged in the sleeve and is slidingly matched with it, and push rod and cam working face join, and push rod, piezoelectric ceramics, insulcrete and loading head fuse; Radially twist mechanism comprises stepper-motor and weighted platform, and weighted platform is fixedlyed connected with step motor shaft; Culturing room comprises culturing room's base, culturing room's body and cultivates chamber cap, culturing room's base and weighted platform are by the contact surface close proximity that rubs, culturing room's body is cup-like structure and fixes with the culturing room base, cultivate chamber cap for falling cup-like structure and tipping upside down on culturing room's body, culture places on culturing room's base, and loading head applies pressure on the culture.
Working mechanism of the present utility model:
The utility model is a kind of deceleration loading device of external structure intervertebral disk, can provide low frequency-high-amplitude and high frequency-reach the by a narrow margin loading procedure that reverses to culture, is divided into low frequency high-amplitude load maintainer, high frequency load maintainer and reverse load maintainer by a narrow margin from function.
The working routine of low frequency high-amplitude load maintainer: stepper-motor drives the cam rotation by camshaft, and cam and push rod are for closely cooperating, and the cam rotation makes push rod move up and down, and culture is applied the compressive load of low frequency high-amplitude; The stroke of cam is adjustable, promptly tighten the rotation center that screw changes cam by changing on the cam, thereby change the eccentricity of cam, realize stroke adjustment, realize adjusting by controller control rotating speed of motor with cam, for different disc tissues provide proper compression load to draught.
High frequency is the working routine of load maintainer by a narrow margin: drive by piezoelectric ceramics power supply, the electrical signal that input is set to piezoelectric ceramics, piezoelectric ceramics produces regular distortion under action of electric signals, give insulcrete with force transmission, insulcrete drives loading head 5 motions, thereby realizes culture is applied high frequency axial loading by a narrow margin.By above-mentioned two mechanisms, will provide near the low frequency high-amplitude-high frequency of physiology stand under load situation coupled double frequency stand under load form by a narrow margin for disc tissue.
Reverse the working routine of load maintainer: the positive and negative rotation that produces counterclockwise and clockwise interaction behind the electrical power, driven by motor weighted platform and culturing room's base twist together, because the frictional force of culture and culturing room's susceptor surface is enough to make itself and culturing room to be reversed simultaneously; The upper end is owing to the vertical pressure of loading head, so culture is similar under the body environment because the twisting action crooked, that twisting motion produced is promptly realized the loading of reversing to culture.The torsional frequency of described motor may be adjusted to the situation when making it near human lumbar intervertebral disk position works better.
Above-mentioned three kinds of mechanisms work after culture is put well simultaneously, simulation lumber ertebral disc growth in vivo environment, be subjected to low frequency high-amplitude-high frequency simultaneously and load and reverse loading by a narrow margin, the mechanical environment of this compound loading is more near human intervertebral disc stand under load form, for the growth of intervertebral disc cells, grow advantageous conditions be provided, this dynamic loading device provides practice condition for external structure functionalization disc tissue.
The beneficial effects of the utility model are: adopt driving system of stepping motor and piezoelectric ceramics shifter that culture is applied and reverse and two kinds of load of high frequency pressurization, near the mechanical environment of human lumber ertebral disc growth and work.The utility model compact construction, volume is little, driving is sensitive, micrometric displacement precision height, can make the distortion of culture reach 25%, thereby cultivating to provide for intervertebral disk meets the required mechanical environment of growth in the body.
Description of drawings
Fig. 1 is the rotation vertebrae disk organizational project bio-reactor sectional structure synoptic diagram of this double frequency compression-radially.
Fig. 2 is the rotation vertebrae disk organizational project bio-reactor one-piece construction synoptic diagram of this double frequency compression-radially.
Among the figure: 1. upper bed-plate 2. lower bottom bases 3. columns 4. motors 5. Cam rests
6. cam 7. camshafts 8. sleeves 9. piezoelectric ceramics 10. push rods 11. insulcretes
12. loading head 13. stepper-motors 14. weighted platforms 15. step motor shafts
16. culturing room's base 17. culturing room's bodies 18. are cultivated chamber cap 19. cultures.
Embodiment
Embodiment:
A kind of double frequency compress-radially reverses disc tissue engineering bio-reactor, comprise deceleration loading device framework, motor-driven mechanism, piezoelectric ceramics load maintainer, radially twist mechanism and culturing room, the deceleration loading device framework is fixedlyed connected by three root posts 3 with lower bottom base 2 by upper bed-plate 1 and is constituted; Motor-driven mechanism comprises motor 4, Cam rest 5, cam 6 and camshaft 7, and motor 4 and Cam rest 5 are fixing with upper bed-plate 1 respectively, and cam 6 is arranged on the Cam rest 5 and by camshaft 7 fixedlys connected with 4 in motor; The piezoelectric ceramics load maintainer comprises sleeve 8, piezoelectric ceramics 9, push rod 10, insulcrete 11 and loading head 12, sleeve 8 is following fixing with upper bed-plate 1, piezoelectric ceramics 9 is arranged in the sleeve 8 and is slidingly matched with it, push rod 10 joins with cam 6 working faces, and push rod 10, piezoelectric ceramics 9, insulcrete 11 and loading head 12 fuse; Radially twist mechanism comprises stepper-motor 13 and weighted platform 14, and weighted platform 14 is fixedlyed connected with step motor shaft 15; Culturing room comprises culturing room's base 16, culturing room's body 17 and cultivates chamber cap 18, culturing room's base 16 and weighted platform 14 are by contact surface friction close proximity, culturing room's body 17 is a cup-like structure and fixing with culturing room base 16, cultivate chamber cap 18 for falling cup-like structure and tipping upside down on culturing room's body 17, culture 19 places on culturing room's base 16, and loading head 12 applies pressure on the culture 19.
Claims (1)
1. a double frequency compress-radially reverses disc tissue engineering bio-reactor, it is characterized in that: comprise deceleration loading device framework, motor-driven mechanism, piezoelectric ceramics load maintainer, radially twist mechanism and culturing room, the deceleration loading device framework is fixedlyed connected by three root posts with lower bottom base by upper bed-plate and is constituted; Motor-driven mechanism comprises motor, Cam rest, cam and camshaft, and motor and Cam rest are fixed with upper bed-plate respectively, and cam is arranged on the Cam rest and by camshaft fixedlys connected with motor shaft; The piezoelectric ceramics load maintainer comprises sleeve, piezoelectric ceramics, push rod, insulcrete and loading head, the following of sleeve and upper bed-plate fixed, piezoelectric ceramics is arranged in the sleeve and is slidingly matched with it, and push rod and cam working face join, and push rod, piezoelectric ceramics, insulcrete and loading head fuse; Radially twist mechanism comprises stepper-motor and weighted platform, and weighted platform is fixedlyed connected with step motor shaft; Culturing room comprises culturing room's base, culturing room's body and cultivates chamber cap, culturing room's base and weighted platform are by the contact surface close proximity that rubs, culturing room's body is cup-like structure and fixes with the culturing room base, cultivate chamber cap for falling cup-like structure and tipping upside down on culturing room's body, culture places on culturing room's base, and loading head applies pressure on the culture.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010205971019U CN201883096U (en) | 2010-11-09 | 2010-11-09 | Double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor |
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| CN2010205971019U CN201883096U (en) | 2010-11-09 | 2010-11-09 | Double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor |
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| CN201883096U true CN201883096U (en) | 2011-06-29 |
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| CN2010205971019U Expired - Fee Related CN201883096U (en) | 2010-11-09 | 2010-11-09 | Double-frequency compression-radial twisting intervertebral disc tissue engineering bioreactor |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103550016A (en) * | 2013-11-11 | 2014-02-05 | 中国人民解放军第三军医大学第一附属医院 | Uniform force applying device for bionic culturing of intervertebral disc |
| CN104046564A (en) * | 2014-06-06 | 2014-09-17 | 上海交通大学 | Physiological environment-imitating mechanical stimulation type biological reactor system |
| CN104388309A (en) * | 2014-09-29 | 2015-03-04 | 中国中医科学院望京医院 | Isolated culture apparatus and loading apparatus for spine movement segment |
| CN104789469A (en) * | 2015-04-29 | 2015-07-22 | 天津理工大学 | Bioreactor for in-vitro cell culture/animal centrifugal high-acceleration loading |
| CN110484426A (en) * | 2019-09-16 | 2019-11-22 | 常州市第一人民医院 | A kind of power stimulation loading device and its working method |
| CN115369037A (en) * | 2022-10-24 | 2022-11-22 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
-
2010
- 2010-11-09 CN CN2010205971019U patent/CN201883096U/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103550016A (en) * | 2013-11-11 | 2014-02-05 | 中国人民解放军第三军医大学第一附属医院 | Uniform force applying device for bionic culturing of intervertebral disc |
| CN103550016B (en) * | 2013-11-11 | 2015-05-27 | 中国人民解放军第三军医大学第一附属医院 | Uniform force applying device for bionic culturing of intervertebral disc |
| CN104046564A (en) * | 2014-06-06 | 2014-09-17 | 上海交通大学 | Physiological environment-imitating mechanical stimulation type biological reactor system |
| CN104046564B (en) * | 2014-06-06 | 2016-01-20 | 上海交通大学 | A kind of physiology environmental mechanics stimulates formula bioreactor system |
| CN104388309A (en) * | 2014-09-29 | 2015-03-04 | 中国中医科学院望京医院 | Isolated culture apparatus and loading apparatus for spine movement segment |
| CN104789469A (en) * | 2015-04-29 | 2015-07-22 | 天津理工大学 | Bioreactor for in-vitro cell culture/animal centrifugal high-acceleration loading |
| CN110484426A (en) * | 2019-09-16 | 2019-11-22 | 常州市第一人民医院 | A kind of power stimulation loading device and its working method |
| CN115369037A (en) * | 2022-10-24 | 2022-11-22 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
| CN115369037B (en) * | 2022-10-24 | 2023-01-31 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110629 Termination date: 20121109 |