CN201825956U - Bioreactor used for in vitro culture of lumbar intervertebral disc - Google Patents
Bioreactor used for in vitro culture of lumbar intervertebral disc Download PDFInfo
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- CN201825956U CN201825956U CN2010205666158U CN201020566615U CN201825956U CN 201825956 U CN201825956 U CN 201825956U CN 2010205666158 U CN2010205666158 U CN 2010205666158U CN 201020566615 U CN201020566615 U CN 201020566615U CN 201825956 U CN201825956 U CN 201825956U
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Abstract
The utility model relates to a bioreactor used for in vitro culture of lumbar intervertebral disc, which comprises a loading device frame, a culture chamber, a torsion transmission mechanism and a pressure transmission mechanism, wherein the loading device frame is formed by the fixed connection of an upper plate, a bottom plate, a main pillar and an auxiliary pillar, a lumbar intervertebral disc culture is placed in the culture chamber, the torsion transmission mechanism comprises a stepping motor and a loading platform which are linked with each other through friction between rough contact surfaces, the pressure transmission mechanism comprises piezoelectric ceramic, a lever, a fulcrum and a pressure lever, the piezoelectric ceramic is arranged in a cylinder body of the main pillar, one end of the lever contacts with a power output working surface of the piezoelectric ceramic, the other end of the lever is fixed with the pressure lever, and the lower end of the pressure lever extends into the culture chamber and exerts pressure on the culture. The bioreactor has the advantages of compact structure, small volume, sensitive driving, and high precision of micrometric displacement; and the stepping motor is adopted to drive a system and a piezoelectric ceramic displacement device to exert two loads of torsion and high-frequency loading on the culture, so as to be close to mechanical environment for growing and working of human lumbar intervertebral disc.
Description
[technical field]
The utility model relates to the biomedical engineering technology field, particularly a kind of bio-reactor that is used for the lumber ertebral disc cultivation.
[background technology]
Fibrocartilaginous morphological element of intervertebral disk and structure and general bone differ widely, and its unique mechanical character plays an important role to flexible motion, the cushion load of backbone, the stability of keeping backbone.The intervertebral disk fibrous cartilage is the important topic that Chinese scholars are studied as the important component part of backbone always.The intervertebral disk fibrous cartilage mainly is carry load, compensator or trimmer pressure and absorption concussion on physiology.In view of its physiological action, its load mode mainly is to compress, stretch and reverse.Human disc is the vesselless tissue of human body maximum, its nutrition supply mainly relies on osmosis, main path has: 1. cartilage endplate approach, promptly nutritive substance by blood vessel in the centrum from medullary space-blood sinus-cartilage endplate interfacial diffusion to intervertebral disk, nutrition nucleus pulposus and fibrous ring internal layer; 2. fibrous ring approach, promptly nutritive substance is by vascular surface nutrition periphery fibrous ring.Mechanics factor plays an important role to the g and D of cartilage, Many researchers has been developed the multiple bio-reactor that mechanical environment is arranged, attempt cultivating the functionalization artificial cartilage, the mechanical condition of these reactors has: fluid shearing stress, liquid pressure, stretching, directly compression, distortion shearing stress or the wherein combination of one or more conditions.Wherein the condition of the cultivation cartilage of fluid shearing stress, liquid pressure and direct compression just is widely studied.
Current, along with the development of organizational project, how deepening continuously of seed cell, timbering material and active factor research is cultivated graft and is subjected to more concern in bio-reactor, wherein the mechanical environment of graft culture condition is more and more paid attention to.For cartilage, its live body mechanical environment is very complicated.Suitable mechanical condition be turn out structure, function all with the important factor of the close graft of natural cartilage.Recent years, correlative study person was to different mechanical stimulations, and the 26S Proteasome Structure and Function of optimization culture has been done big quantity research.Based on above factor, our lumber ertebral disc bio-reactor is intended to the external mechanical environment that lumber ertebral disc growth is provided, provide with body in identical load mode under the working condition, go out to have the intervertebral disk that certain force is learned performance in vitro culture.
The piezoelectric ceramics displacement mechanism has compact construction, volume is little, nothing rubs, drive the characteristics sensitive, that the micrometric displacement precision is high, in addition, use the piezoelectric ceramics shifter, because the speed that electromechanical Coupling is carried out is very fast, there is not heating problem, the electricity that influences culturing cell is led with thermal radiation and can be ignored, because the piezoelectric ceramics shifter is the structure similar to electrical condenser, so minimum power consumption is arranged, even under high speed and high voltage drive situation, also be like this, therefore, the piezoelectric ceramics shifter can be used as the power resources that artificial cartilage is cultivated dynamic load.Though the displacement that the piezoelectric ceramics shifter produces is less, the amplification by leverage improves load deflection, can satisfy loading (distortion) needs that cartilage is cultivated.As 200 microns of piezoelectric ceramics shifter maximum drive displacements, through 10 times of amplifications of lever, drive displacement can reach 2 millimeters.Thicker cartilage is by 8 mm thick, and its distortion can reach 25%.Usually the mechanical environment of cartilage cultivation is distortion 5%-20%, and intervertebral disk and cartilage are similar.Can form compact construction, volume is little, driving is sensitive, the micrometric displacement precision is high dynamic loading device like this, add suitable filling system and might form the bio-reactor that intervertebral disk is cultivated.
[utility model content]
The purpose of this utility model is according to existing knowledge and mechanical condition, provides a kind of deceleration loading device that can realize lumber ertebral disc is carried out vitro culture at the disease of human lumber ertebral disc aspect.
The technical solution of the utility model:
A kind of bio-reactor that is used for the lumber ertebral disc vitro culture, comprise deceleration loading device framework, culturing room, reverse transmission rig and pressure drive mechanism, the deceleration loading device framework is fixedlyed connected with secondary column by head tree with base plate by upper plate and is constituted, and head tree is a tube structure; Culturing room comprises at the bottom of culturing room's body and the culturing room and by coarse surface in contact friction interlock, the lumber ertebral disc culture places in the culturing room, is provided with nutrition at the bottom of the culturing room and fills joint; Reverse transmission rig and comprise stepper-motor and weighted platform, stepper-motor is connected with weighted platform by output shaft, and stepper-motor and base plate are fixed, and the lower surface at the bottom of the culturing room contacts with the upper surface of weighted platform and by coarse surface in contact friction interlock; Pressure drive mechanism comprises piezoelectric ceramics, lever, fulcrum and depression bar, piezoelectric ceramics is contained in the cylindrical shell of head tree and with the head tree inwall and forms movingly, the bottom and the base plate of piezoelectric ceramics are fixed, lever and fulcrum pass through screw retention, one end of lever contacts with the power output services face of piezoelectric ceramics and applies pressure to the upper surface of fulcrum by the adjusting screw(rod) that is arranged at upper plate and makes its contact closely, the other end of lever is fixed by screw and depression bar, upper plate and can sliding is passed in the upper end of depression bar in perforation, the lower end of depression bar extend in the culturing room and applies pressure on the lumber ertebral disc culture.
Principle of work of the present utility model:
The utility model is a kind of deceleration loading device of lumber ertebral disc external structure, can provide simultaneously culture to reverse and pressurization.The electrical signal that input is set to piezoelectric ceramics, piezoelectric ceramics produces regular distortion under action of electric signals, the distortion of piezoelectric ceramics is exaggerated and is delivered to depression bar by lever, depression bar acts on the culture in the culturing room, thereby realizes the high frequency of the disc tissue vertical direction cultivated is loaded.Give the stepper-motor input electrical signal, this signal makes motor produce the rotation of counterclockwise and clockwise interaction, driven by motor weighted platform and culturing room twist together, because the frictional force of culture lower end and culturing room bottom is enough to make itself and culturing room to be reversed simultaneously, the vertical pressure of upper end compression rod 3, therefore make the culture that is fixed on the weighted platform be similar to lumber ertebral disc reversing under the growing state in vivo, and then realization is to the loading of reversing of culture.
The beneficial effects of the utility model are: adopt driving system of stepping motor and piezoelectric ceramics shifter that culture is applied to reverse with high frequency and load two kinds of load, near the mechanical environment of human lumber ertebral disc growth and work.The utility model compact construction, volume is little, driving is sensitive, micrometric displacement precision height, especially adopt the piezoelectric ceramics shifter as the deceleration loading device power resources, by leverage the load sphere of action is amplified again, can make the distortion of culture reach 25%, thereby meet the required mechanical environment of growth in the body for the intervertebral disk cultivation provides.
[description of drawings]
Accompanying drawing is this bioreactor construction synoptic diagram.
Among the figure: 1. 15. weighted platforms, 16. stepper-motors at the bottom of secondary column 2. upper plates 3. depression bars 4. screws 5. levers 6. adjusting screw(rod)s 7. screws 8. fulcrums 9. piezoelectric ceramics 10. head trees 11. base plates 12. joints 13. culturing room's bodies 14. culturing room
[embodiment]
Embodiment:
A kind of bio-reactor that is used for the lumber ertebral disc vitro culture, comprise deceleration loading device framework, culturing room, reverse transmission rig and pressure drive mechanism, the deceleration loading device framework is fixedlyed connected with secondary column 1 by head tree 10 with base plate 11 by upper plate 2 and is constituted, and head tree 10 is a tube structure; Culturing room comprises at the bottom of culturing room's body 13 and the culturing room 14 and by coarse surface in contact friction interlock, and the lumber ertebral disc culture places in the culturing room, and 14 are provided with nutrition and fill joint 12 at the bottom of the culturing room; Reverse transmission rig and comprise stepper-motor 16 and weighted platform 15, stepper-motor 16 is connected with weighted platform 15 by output shaft, stepper-motor 16 is fixing with base plate 11, and 14 lower surface contact with the upper surface of weighted platform 15 and passes through the coarse surface in contact interlock that rubs at the bottom of the culturing room; Pressure drive mechanism comprises piezoelectric ceramics 9, lever 5, fulcrum 8 and depression bar 3, piezoelectric ceramics 9 is contained in the cylindrical shell of head tree 10 and with head tree 10 inwalls and forms movingly, the bottom of piezoelectric ceramics 9 and base plate 11 are fixing, lever 5 and fulcrum 8 are fixing by screw 7, one end of lever 5 contacts with the power output services face of piezoelectric ceramics 9 and applies pressure to the upper surface of fulcrum 8 by the adjusting screw(rod) 6 that is arranged at upper plate 2 and makes its contact closely, the other end of lever 5 is fixing with depression bar 3 by screw 4, the upper end of depression bar 3 is passed upper plate 2 and can be slided in perforation, and the lower end of depression bar 5 extend in the culturing room and applies pressure on the lumber ertebral disc culture.
Claims (1)
1. bio-reactor that is used for the lumber ertebral disc vitro culture, it is characterized in that: comprise deceleration loading device framework, culturing room, reverse transmission rig and pressure drive mechanism, the deceleration loading device framework is fixedlyed connected with secondary column by head tree with base plate by upper plate and is constituted, and head tree is a tube structure; Culturing room comprises at the bottom of culturing room's body and the culturing room and by coarse surface in contact friction interlock, the lumber ertebral disc culture places in the culturing room, is provided with nutrition at the bottom of the culturing room and fills joint; Reverse transmission rig and comprise stepper-motor and weighted platform, stepper-motor is connected with weighted platform by output shaft, and stepper-motor and base plate are fixed, and the lower surface at the bottom of the culturing room contacts with the upper surface of weighted platform and by coarse surface in contact friction interlock; Pressure drive mechanism comprises piezoelectric ceramics, lever, fulcrum and depression bar, piezoelectric ceramics is contained in the cylindrical shell of head tree and with the head tree inwall and forms movingly, the bottom and the base plate of piezoelectric ceramics are fixed, lever and fulcrum pass through screw retention, one end of lever contacts with the power output services face of piezoelectric ceramics and applies pressure to the upper surface of fulcrum by the adjusting screw(rod) that is arranged at upper plate and makes its contact closely, the other end of lever is fixed by screw and depression bar, upper plate and can sliding is passed in the upper end of depression bar in perforation, the lower end of depression bar extend in the culturing room and applies pressure on the lumber ertebral disc culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205666158U CN201825956U (en) | 2010-10-19 | 2010-10-19 | Bioreactor used for in vitro culture of lumbar intervertebral disc |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205666158U CN201825956U (en) | 2010-10-19 | 2010-10-19 | Bioreactor used for in vitro culture of lumbar intervertebral disc |
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| Publication Number | Publication Date |
|---|---|
| CN201825956U true CN201825956U (en) | 2011-05-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2010205666158U Expired - Fee Related CN201825956U (en) | 2010-10-19 | 2010-10-19 | Bioreactor used for in vitro culture of lumbar intervertebral disc |
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| CN (1) | CN201825956U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555579A (en) * | 2013-11-11 | 2014-02-05 | 中国人民解放军第三军医大学第一附属医院 | Multifunctional bionic tissue culture device |
| CN104046564A (en) * | 2014-06-06 | 2014-09-17 | 上海交通大学 | Physiological environment-imitating mechanical stimulation type biological reactor system |
| CN108843639A (en) * | 2018-06-04 | 2018-11-20 | 江苏理工学院 | A kind of novel piezoelectric ceramic type jet action valve valve group |
| CN115369037A (en) * | 2022-10-24 | 2022-11-22 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
-
2010
- 2010-10-19 CN CN2010205666158U patent/CN201825956U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555579A (en) * | 2013-11-11 | 2014-02-05 | 中国人民解放军第三军医大学第一附属医院 | Multifunctional bionic tissue culture device |
| CN103555579B (en) * | 2013-11-11 | 2014-12-17 | 中国人民解放军第三军医大学第一附属医院 | Multifunctional bionic tissue culture device |
| CN104046564A (en) * | 2014-06-06 | 2014-09-17 | 上海交通大学 | Physiological environment-imitating mechanical stimulation type biological reactor system |
| CN104046564B (en) * | 2014-06-06 | 2016-01-20 | 上海交通大学 | A kind of physiology environmental mechanics stimulates formula bioreactor system |
| CN108843639A (en) * | 2018-06-04 | 2018-11-20 | 江苏理工学院 | A kind of novel piezoelectric ceramic type jet action valve valve group |
| CN108843639B (en) * | 2018-06-04 | 2020-07-28 | 江苏理工学院 | Piezoelectric ceramic type jet pipe valve group |
| CN115369037A (en) * | 2022-10-24 | 2022-11-22 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
| CN115369037B (en) * | 2022-10-24 | 2023-01-31 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110511 Termination date: 20121019 |