CN201149589Y - Large displacement cell strain loading device under three-dimensional cultivation condition - Google Patents
Large displacement cell strain loading device under three-dimensional cultivation condition Download PDFInfo
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- CN201149589Y CN201149589Y CNU2008200281715U CN200820028171U CN201149589Y CN 201149589 Y CN201149589 Y CN 201149589Y CN U2008200281715 U CNU2008200281715 U CN U2008200281715U CN 200820028171 U CN200820028171 U CN 200820028171U CN 201149589 Y CN201149589 Y CN 201149589Y
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Abstract
The utility model discloses a large displacement cell strain loading device in three-dimensional culture conditions, a power supply is connected to electrodes of a bimorph, the electrodes are positioned at both sides of the bimorph and in tight junction with an electric wafer, a third electrode is led out from a copper piece of an intermediate layer of the electric wafer, the bimorph is fixed on a substrate, the rear end of a displacement transferring plunger is fixed with the displacement output end of the bimorph, the front end thereof is connected with a porous fixed material of a three-dimensional compound body composed of a cell support; and the other side of the three-dimensional compound body composed of the cell support is fixed on the wall of a culture chamber through the porous fixed material, and a laser probe is non-contact mounted on the rear end of the transferring plunger and detects the backward and forward displacement of the transferring plunger. The loading device can load the amplitude and frequency without closing down the machine and can be adjusted conveniently; and has advantages of good maneuverability, high repeatability precision, stable mechanical loading and low error, avoiding magnetic-field interference, mature technique and rather large displacement output.
Description
Technical field
The utility model relates to a kind of determinator that is used for biomechanics Research, and cell is accepted the loading experimental apparatus of mechanical stress stimulation under particularly a kind of three-dimensional cultivation condition.
Background technology
In recent years, increasing along with crossing research between life science and other subjects, the new branch of science that biomechanics is carried out quantitative examination as applied mechanics principle and method to the mechanics problem in the biosome becomes the focus of field of biology research gradually.Wherein cell is one of field, forward position of present biomechanics Research to the response investigations of mechanical stimulation.Yet because the human body internal milieu is extremely complicated, the comparatively difficulty that research also becomes in the body of this aspect.And multiple isolated cells culture technique provides possibility for the biological effect of studying mechanical stimulation on the cell aspect.Studies show that mechanical stimulation can cause cytoskeleton to produce deformation, cause the variation of skeleton arrangement, and then influence the many aspects such as mechanical property, adhesive force, propagation, differentiation of cellular morphology, cell self.
Many isolated cells Experiments of Machanics methods have been studied personnel's proposition and have been used for the research of biosome to the mechanism aspects such as response of mechanics factor, as substrate tension, four-point bending stretching, hydrostatic pressure and fluid shear stress stimulation separately, the combination of fluid shear stress etc.Also set up simultaneously the model that many mechanics load.Publication number is a kind of cyto-mechanics loading system that the Chinese patent of CN 1847847 discloses, this system is made of the culture chamber that the bottom is fixed with pellosil, can inoculating cell on the pellosil, hoisting gasbag is arranged at the pellosil bottom, by adjusting how many generation distortion of air bag internal gas,, pellosil produces planar equal-biaxial periodical pull stress or compressive stress thereby being out of shape.Though this device is simple to operate, volume is little, the distortion that external force produces air bag is closely related with the original pressure of its internal gas, is difficult to accomplish therefore that very accurate control air bag is out of shape; Secondly the deformation that cell receives in this device does not have direct detection system.Publication number be CN 1425905 patented invention a kind of four point bending cell mechanics loader, this device is by the rotation of stepper motor, drive drive screw and produce the front and back displacement, promote pressure head (two contact points respectively being arranged up and down) and load the culture plate that inoculation has cell, make culture plate and cell produce distortion with Tissue Culture Plate.This device controllability is good, the repeatable accuracy height, owing to need to use but device is comparatively complicated, and because the deformation difference of culture plate diverse location produces identical deformation so can not guarantee cell.Publication number be CN 1932510 patent disclosure a kind of charger of cell tensile, it passes through controller, the driver control stepper motor rotates, rotation is changed into the displacement of horizontal direction by screw mandrel, the pulling inoculation has the culture membrane of cell, make cell be subjected to the stretcher strain of horizontal direction, this device can make cell stressed comparatively even, accurately control stretching amplitude and frequency, stretching distance also can be regulated easily, but use stepper motor in this device as drive source, inevitably bring the interference in magnetic field, and magnetic field is still indeterminate at present as a kind of influence of physical factor pair cell, studies cell for us the response of mechanical stimulation is brought disturbing factor.
Above-mentioned three disclosed devices of patent all are based upon cell monolayer and cultivate on the basis, the cultured cell monolayer growth, comparatively convenient when carrying out as experiments such as morphologic observation, cell dyeings, but the residing solid space of cell under the shortage internal milieu, weakened influencing each other and acting on of iuntercellular autocrine, paracrine, therefore and the environment of body cell when being subjected to mechanical stimulation is 3 D stereo, should set up the research model of cell under the 3 D stereo condition during research aspect carrying out this.Publication number be CN 1567399 patent disclosure cardiac muscle cell's mechanical stimulation device under a kind of three-dimensional cultivation condition, this device utilizes method of tissue engineering to make up cell--the complex of collagen, form the dimensional culture environment of cell, by motor, flywheel, connecting rod and slide block the rotation of motor is become motion on the horizontal direction again, periodic cell--the complex of collagen that loads makes cell be subjected to mechanical stimulation.Though this device has been realized the three dimensional growth environment of cell, it drives the motor that produces magnetic field when still using work, and its adjusting that loads amplitude is needed to shut down, and brings inconvenience to operation; In addition, this device adopts too much mechanical drive, and also there is the wear problem in the long-term work in unavoidable noise and the heat of producing during work.
Summary of the invention
For overcome the prior art complex structure, have magnetic field, the deficiency of aspects such as disturbing factor such as heat; the utility model provides cell or tissue strain loading device under a kind of three-dimensional cultivation condition; simple in structure; can not shut down and change parameters such as loading amplitude, frequency; avoid magnetic interference; the generation heat is low, is applicable to the mechanics loading experimental apparatus of cell under the three-dimensional cultivation condition.
The technical scheme that its technical matters that solves the utility model adopts is: adopt bimorph as drive source, the three-dimensional complex that pair cell--porous support constitutes carries out mechanical stimulation.The utility model is primarily aimed at that pair cell carries out mechanical stimulation under the three-dimensional cultivation condition, comprises bimorph driving power, bimorph drive part, cell culture unit and laser displacement sensor deformation test section.Adopt bimorph to be because its technology maturation, exportable than big displacement.The bimorph drive part mainly is made of its core component bimorph, electrode, electric wafer stationary installation, transmission plunger, porous immobilization material; Cell culture unit is made of substrate and cultivation cell; Laser displacement sensor deformation test section is made up of laser probe and computing machine.Power supply is connected to by drive wire on the electrode of bimorph drive part, and electrode is positioned at the both sides of bimorph, closely is connected with electric wafer, and the 3rd electrode drawn from electric wafer middle layer copper sheet.By electric wafer stationary installation bimorph is fixed in the substrate, the displacement output terminal that plunger back-end and bimorph are transmitted in displacement is fixed together, front end be connected cell--the porous immobilization material that props up the three-dimensional complex that is configured to is fixed.Cell--a three-dimensional complex that is configured to is positioned at cultivates cell bottom center position, about connect the porous immobilization material respectively, the porous immobilization material of a side is fixed on to be cultivated on the little locular wall, the porous immobilization material of opposite side is fixedlyed connected with displacement transmission plunger.The laser probe noncontact is installed on the transmission plunger back-end, links to each other with computing machine, detects the front and back displacement of transmitting plunger.
Described porous immobilization material is not for producing Cytotoxic porosint, as porous polyethylene etc.
Cell culture unit adopts organic glass or polytetrafluoroethylmaterial material to make in the utility model, is easy to processing, also can sterilize easily simultaneously, prevents that possible pollution from taking place.
Principle of work of the present utility model: the bimorph driving power provides the voltage of sinusoidal wave or other waveforms of bimorph needs, the flexural deformation that bimorph produces or the anteroposterior position Mobile Communication of piezo-electric stack driver cross displacement transmission plunger and are delivered to cell--on the porous support complex, cell--porous support complex two ends condense together with the porous immobilization material respectively, so that can produce compression or stretcher strain when plunger seesaws, and then make cell produce periodic deformation.By with transmit the laser probe that the plunger noncontact is installed, detect the displacement that transmits plunger, data transfer by the processing of computer software, shows the displacement waveform to computing machine in real time.
The beneficial effects of the utility model are: the utility model can pair cell applies the deformation of waveforms such as sine wave or triangular wave, frequency range is 0-100Hz, amplitude of deformation is that maximum can reach 1mm, and adopt the method that makes up porous support and cell conjugate to provide the cell growth required three-dimensional environment, farthest simulated the growing environment in the cell body.This device adopts bimorph as the power that loads cell, and can under non-stop-machine situation, load amplitude, frequency, and can regulate easily, and its technology maturation, exportable than big displacement.
Stretching, compressive strain that the output of cyto-mechanics loading experimental apparatus in the utility model produces various ways, it is good to have operability, the repeatable accuracy height, mechanics loads steadily, and error is little, the magnetic interference of having brought when having avoided stepper motor as drive source.The characteristics such as 3 D stereo culture environment of cell growth needs can be provided, the deformation that cell is produced, the experiment under the same terms has good consistance.Can be used as pair cell in the biomechanics and carry out the experimental provision of mechanical stimulation response investigations.
Below in conjunction with drawings and Examples the utility model is further specified.
Description of drawings
Fig. 1 cell three-dimensional described in the utility model is cultivated the synoptic diagram of strain loading device;
Fig. 2 is cell culture unit described in the utility model and bimorph drive part side view;
Among the figure, the 1-substrate; The 2-lead-in wire picks out end; The 3-lead-in wire; 4-piezoelectric ceramics cutting ferrule; The 5-bimorph; 6-cultivates cell; The 7-driving ram; The complex of 8-collagen scaffold and cell; 9-porous immobilization material; The 10-laser probe; The 11-computing machine; The 12-drive power supply for piezoelectric ceramics;
Fig. 3 is the schematic three dimensional views that cell three-dimensional described in the utility model is cultivated strain loading device.
Embodiment
Embodiment:
With reference to Fig. 1, Fig. 2 and Fig. 3, the utility model device comprises bimorph driving power, bimorph drive part, cell culture unit and laser displacement sensor deformation test section.Bimorph driving power 12 provides on-load voltage; The bimorph drive part mainly is made of its core component bimorph 5, electrode, electric wafer stationary installation, transmission plunger 7, porous immobilization material; Cell culture unit is made of substrate 1 and cultivation cell 6; Laser displacement sensor deformation test section is made up of laser probe 10 and computing machine 11.Wherein electrode picks out end 2 for lead-in wire, electricity wafer stationary installation is selected piezoelectric ceramics cutting ferrule 4 for use, the porous immobilization material uses porous polyethylene 9, and substrate 1, piezoelectric ceramics cutting ferrule 4, cellular incubation cell 6 are pmma material, and driving ram 7 materials are teflon.Substrate 1 is the base of this device, and lead-in wire picks out end 2 and cultivates cell 6 and is fixed in the substrate 1.Bimorph 5 is fixed on cultivation cell 6 front sides by piezoelectric ceramics cutting ferrule 4, and lead-in wire 3 is connected to lead-in wire and picks out on the end 2, and power supply 12 applies voltage by the 3 pairs of bimorphs 5 that go between, and makes it produce front and back deformation.Polymerization can take place when raising in the collagen temperature, is condensed into one with porous polyethylene 9, and the deformation that driving ram 7 transmits is loaded on the complex 8 of collagen scaffold and cell.When needs carry out the cyto-mechanics loading, regulate power supply 12 earlier, output needs the voltage of waveform, voltage 3 is loaded on the bimorph 5 by going between, because bimorph 5 two ends are fixed on the cellular incubation cell 6, so the position, intermediate portion can produce maximum deformation, is delivered to porous polyethylene 9 by driving ram 7, and then make the complex 8 of collagen scaffold and cell produce distortion.
Claims (3)
1, big displacement cell strain loading device under the three-dimensional cultivation condition, comprise bimorph driving power, bimorph drive part, cell culture unit and laser displacement sensor deformation test section, it is characterized in that: described bimorph drive part comprises bimorph, electrode, electric wafer stationary installation, transmits plunger, porous immobilization material; Cell culture unit is made of substrate and cultivation cell; Laser displacement sensor deformation test section comprises laser probe and computing machine; Power supply is connected to by drive wire on the electrode of bimorph drive part, and electrode is positioned at the both sides of bimorph, closely is connected with electric wafer, and the 3rd electrode drawn from electric wafer middle layer copper sheet; By electric wafer stationary installation bimorph is fixed in the substrate, the displacement output terminal that plunger back-end and bimorph are transmitted in displacement is fixed together, front end be connected cell--the porous immobilization material that props up the three-dimensional complex that is configured to is fixed; Cell--a three-dimensional complex that is configured to is positioned at cultivates cell bottom center position, about connect the porous immobilization material respectively, the porous immobilization material of a side is fixed on to be cultivated on the little locular wall, the porous immobilization material of opposite side is fixedlyed connected with displacement transmission plunger; The laser probe noncontact is installed on the transmission plunger back-end, links to each other with computing machine, detects the front and back displacement of transmitting plunger.
2, according to utilizing big displacement cell strain loading device under the described three-dimensional cultivation condition of claim 1, it is characterized in that: described porous immobilization material is not for producing Cytotoxic porosint.
3, according to utilizing big displacement cell strain loading device under the described three-dimensional cultivation condition of claim 1, it is characterized in that: described cell culture unit adopts organic glass or polytetrafluoroethylmaterial material to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200281715U CN201149589Y (en) | 2008-01-22 | 2008-01-22 | Large displacement cell strain loading device under three-dimensional cultivation condition |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200281715U CN201149589Y (en) | 2008-01-22 | 2008-01-22 | Large displacement cell strain loading device under three-dimensional cultivation condition |
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| CN201149589Y true CN201149589Y (en) | 2008-11-12 |
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| CNU2008200281715U Expired - Fee Related CN201149589Y (en) | 2008-01-22 | 2008-01-22 | Large displacement cell strain loading device under three-dimensional cultivation condition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101221166B (en) * | 2008-01-22 | 2011-09-14 | 西北工业大学 | Cell strain loading device under three-dimensional cultivation condition |
| CN103355187A (en) * | 2012-04-09 | 2013-10-23 | 上海市计划生育科学研究所 | Animal model manufacturing device with magnetic field generating device |
| CN105199955A (en) * | 2015-10-22 | 2015-12-30 | 重庆大学 | Three-dimensional cell culture device capable of performing mechanical-chemical coupling loading |
-
2008
- 2008-01-22 CN CNU2008200281715U patent/CN201149589Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101221166B (en) * | 2008-01-22 | 2011-09-14 | 西北工业大学 | Cell strain loading device under three-dimensional cultivation condition |
| CN103355187A (en) * | 2012-04-09 | 2013-10-23 | 上海市计划生育科学研究所 | Animal model manufacturing device with magnetic field generating device |
| CN103355187B (en) * | 2012-04-09 | 2015-03-25 | 上海市计划生育科学研究所 | Animal model manufacturing device with magnetic field generating device |
| CN105199955A (en) * | 2015-10-22 | 2015-12-30 | 重庆大学 | Three-dimensional cell culture device capable of performing mechanical-chemical coupling loading |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081112 Termination date: 20120122 |