CN103184144B - Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device - Google Patents
Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device Download PDFInfo
- Publication number
- CN103184144B CN103184144B CN201310093826.2A CN201310093826A CN103184144B CN 103184144 B CN103184144 B CN 103184144B CN 201310093826 A CN201310093826 A CN 201310093826A CN 103184144 B CN103184144 B CN 103184144B
- Authority
- CN
- China
- Prior art keywords
- deflecting wheel
- support arm
- geometrical clamp
- cell
- displacement support
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Sampling And Sample Adjustment (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device. The loading device comprises a base film for the growth of an adherent cell used for being observed; two fixing clips for clamping the two sides of the base film respectively, two displacement support arms for supporting the fixing clips respectively, and a driving part, wherein the fixing clips are movably arranged on a rail, and the driving part is used for driving the displacement support arms to perform symmetrical opposite-direction displacement on the rail so as to conduct bidirectional-stretch on the base film. According to the invention, by conducting the stretch with a certain frequency and a certain range on the cell growing on the biocompatible film, morphologic change, cytoskeleton reorganization, signal transduction and other responses of the cell in different action times can be observed.
Description
Technical field
The present invention relates to a kind of cell device for biomethanics, Mechanobiological coupling and biomedical research.
Background technology
The announcement of Stress-growth relationship is the great discovery of biomethanics, and cell is the elementary cell of life.And distraction force is the principal element determining cells deformation and function, therefore, the key issue experimental provision of Cells In Vitro applying elongation strain being become to the research of solution Mechanobiological coupling is developed.
Along with going deep into of studying mechanical stress regulating cell biological function, develop multiple device cell in vitro being carried out to tensile loads, mainly include vacuum (negative pressure) charger, charger such as four_point bending beam such as strain loading device such as liquid (malleation) pressue device, twin shaft etc., two-way strain loading device etc., but all cannot realize the object to attached cell dynamic load online observation.
Summary of the invention
For prior art Problems existing, the object of the present invention is to provide a kind of cell biomechanics charger of dynamic bidirectional stretching original position online observation, can realize attached cell dynamic load online observation.
The cell biomechanics charger of a kind of dynamic bidirectional stretching original position online observation of the present invention comprises:
Basilar memebrane, grows the attached cell for observing;
Geometrical clamp, described geometrical clamp is 2, respectively the both sides of clamp base film;
Displacement support arm, described displacement support arm is 2, and for supporting described geometrical clamp respectively, described geometrical clamp is removably set on guide rail;
Drive division, does symmetrical opposite direction for driving described displacement support arm and moves, to carry out biaxial tension to described basilar memebrane on described guide rail.
Preferably, described drive division comprises: drive motors, bending and stretching arm, deflecting wheel chute, deflecting wheel and electric machine support; Drive motors is arranged on electric machine support, and deflecting wheel is arranged on the clutch end of described drive motors; One end of bending and stretching arm is rotatably arranged on described displacement support arm, and the other end is rotatably arranged on described deflecting wheel chute; Described deflecting wheel is embedded in described deflecting wheel chute.
Preferably, described geometrical clamp one of them be arranged on described displacement support arm by microspur adjusting slider.
Preferably, the microspur adjusting knob regulating the displacement of described microspur adjusting slider is also comprised.
The present invention, by applying the stretching of certain frequency certain amplitude to the cell that is grown on non-biocompatible thin film's (basilar memebrane), can investigate the responses such as the metamorphosis of cell under different action time, skeleton restructuring and intracellular signaling.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Detailed description of the invention
As shown in Figure 1, the present invention includes: basilar memebrane 2, geometrical clamp 3, displacement support arm 4 and drive division 7.Basilar memebrane 2 grows the attached cell for observing.Geometrical clamp 3 is 2, respectively the both sides of clamp base film 2.Displacement support arm 4 is also 2, and corresponding and geometrical clamp 3 is arranged, and for supporting geometrical clamp 3 respectively, geometrical clamp 3 is removably set on guide rail 6.Drive division 7 does symmetrical opposite direction for drive displacement support arm 4 and moves, to carry out biaxial tension to basilar memebrane 2 on guide rail 6.
As shown in Figure 1, in embodiments of the present invention, drive division 7 comprises: drive motors 71, bending and stretching arm 75, deflecting wheel chute 73, deflecting wheel 74 and electric machine support 72; Drive motors 71 is arranged on electric machine support 3, and deflecting wheel 74 is arranged on the clutch end of drive motors 71.One end of bending and stretching arm 75 is rotatably arranged on displacement support arm 5, and the other end is rotatably arranged on deflecting wheel chute 73.Deflecting wheel 74 is embedded in deflecting wheel chute 73.
Like this, when drive motors 71 drives deflecting wheel 74 to rotate time, deflecting wheel 74 just drives deflecting wheel chute 73 to move in the direction of arrow A, makes bending and stretching arm 75 drive displacement support arm 4 on guide rail 6, do symmetrical opposite direction and moves, thus pull basilar memebrane 2.
Because displacement support arm 4 does symmetrical opposite direction to move, just can ensure that the intermediate point on basilar memebrane 2 is roughly static in the process stretched, like this, basilar memebrane 2 linearly does biaxial tension campaign under certain frequency certain amplitude, is subject to the effect of tensile force strain under the effect that the attached cell that just can In-situ basilar memebrane 2 middle section be grown by microscope is stretched at basilar memebrane 2.
In addition, drive motors 71 drives deflecting wheel 74 to do eccentric motion, and the amplitude of eccentric motion determines to seesaw the size of distance.
In addition, as shown in Figure 1, for the ease of launch basilar memebrane 2, geometrical clamp 3 one of them be arranged on displacement support arm 5 by microspur adjusting slider 8, microspur adjusting slider 8 regulates displacement by microspur adjusting knob 9.
When using, as shown in Figure 1, basilar memebrane 2 is immersed in culture dish 1, basilar memebrane 2 grows the attached cell for observing, then by the centre position of microscope alignment basilar memebrane 2, start drive motors 71, just can carry out dynamic bidirectional stretching original position online observation.
The foregoing is only preferred embodiment of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (2)
1. a cell biomechanics charger for dynamic bidirectional stretching original position online observation, is characterized in that, comprising:
Basilar memebrane, grows the attached cell for observing;
Geometrical clamp, described geometrical clamp is 2, respectively the both sides of clamp base film;
Displacement support arm, described displacement support arm is 2, and for supporting described geometrical clamp respectively, described geometrical clamp is removably set on guide rail;
Drive division, does symmetrical opposite direction for driving described displacement support arm and moves, to carry out biaxial tension to described basilar memebrane on described guide rail; Described drive division comprises: drive motors, bending and stretching arm, deflecting wheel chute, deflecting wheel and electric machine support; Drive motors is arranged on electric machine support, and deflecting wheel is arranged on the clutch end of described drive motors; One end of bending and stretching arm is rotatably arranged on described displacement support arm, and the other end is rotatably arranged on described deflecting wheel chute; Described deflecting wheel is embedded in described deflecting wheel chute; Described geometrical clamp one of them be arranged on described displacement support arm by microspur adjusting slider.
2. device as claimed in claim 1, is characterized in that, also comprises the microspur adjusting knob regulating the displacement of described microspur adjusting slider.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310093826.2A CN103184144B (en) | 2013-03-22 | 2013-03-22 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310093826.2A CN103184144B (en) | 2013-03-22 | 2013-03-22 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103184144A CN103184144A (en) | 2013-07-03 |
| CN103184144B true CN103184144B (en) | 2015-04-29 |
Family
ID=48675595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310093826.2A Active CN103184144B (en) | 2013-03-22 | 2013-03-22 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103184144B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867888A (en) * | 2017-03-20 | 2017-06-20 | 北京理工大学 | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time |
| CN109468225A (en) * | 2018-11-12 | 2019-03-15 | 太原理工大学 | A kind of cell mechanical tensioning devices of real time data feedback |
| CN111117883B (en) * | 2019-03-28 | 2020-12-25 | 北京茵维德生物科技有限公司 | Biological intelligent cell dynamic culture system |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101092595B (en) * | 2006-06-23 | 2010-09-08 | 上海交通大学医学院附属第九人民医院 | Experimental apparatus for loading cell through digital controlled mechanical strain |
| CN100514059C (en) * | 2006-09-22 | 2009-07-15 | 重庆大学 | Sinusoidal tensile cell loader |
| CN1932510B (en) * | 2006-09-22 | 2010-05-12 | 重庆大学 | Cell tensile loader |
| CN201008641Y (en) * | 2006-12-31 | 2008-01-23 | 林恩赐 | Massager of massage chair |
| CN200999244Y (en) * | 2007-01-09 | 2008-01-02 | 重庆大学 | Cell substrate uniaxial drawing apparatus |
| CN201261787Y (en) * | 2008-06-13 | 2009-06-24 | 国家纳米科学中心 | Cell cultivation apparatus for exerting mechanical stimulation on cell |
| CN101298592B (en) * | 2008-06-16 | 2011-06-01 | 重庆大学 | Cell three-dimensional mechanical loading unit |
| CN101649291B (en) * | 2009-08-25 | 2011-12-21 | 四川大学 | Extension and compression device of multi-unit cells |
| CN201737929U (en) * | 2010-07-09 | 2011-02-09 | 东南大学 | Precision visualization cell stretching device under environment simulating inner environment of human body |
-
2013
- 2013-03-22 CN CN201310093826.2A patent/CN103184144B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103184144A (en) | 2013-07-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1932510B (en) | Cell tensile loader | |
| CN103184144B (en) | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device | |
| CN101221166B (en) | Cell strain loading device under three-dimensional cultivation condition | |
| CN107294422B (en) | Two-way multistable dielectric elastomer driver | |
| CN106867888A (en) | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time | |
| Culebras et al. | Optimization of cell growth on bacterial cellulose by adsorption of collagen and poly-L-lysine | |
| Huang et al. | A biomimetic bilayer hydrogel actuator based on thermoresponsive gelatin methacryloyl–poly (N-isopropylacrylamide) hydrogel with three-dimensional printability | |
| CN101906379A (en) | Device for precisely stretching visual cells under simulated in vivo environment | |
| Davidson et al. | Fiber crimp confers matrix mechanical nonlinearity, regulates endothelial cell mechanosensing, and promotes microvascular network formation | |
| Gu et al. | Mechanical stimulation of cells with electroactive polymer-based soft actuators | |
| KR102124420B1 (en) | Method for controlling microfilament alignment in structure by pre-strain combination and physical properties | |
| Zhao et al. | In vitro cell stretching devices and their applications: From cardiomyogenic differentiation to tissue engineering | |
| CN105199955A (en) | Three-dimensional cell culture device capable of performing mechanical-chemical coupling loading | |
| CN201737929U (en) | Precision visualization cell stretching device under environment simulating inner environment of human body | |
| Kim et al. | Dynamic cell culture device using electroactive polymer actuators with composite electrodes to transfer in-plane mechanical strain to cells | |
| EP1990402A1 (en) | Bioreactor to apply mechanical forces as an anabolic stimulus | |
| JP6801856B2 (en) | Grip | |
| CN103966094B (en) | A kind of culture apparatus and method acting on cell tensile stress | |
| CN115627234A (en) | Cell traction device capable of being carried on optical microscope to perform dynamic cell imaging | |
| JP5745589B2 (en) | Cell culture plate with culture equipment | |
| CN217868788U (en) | Novel cell stretching cavity structure of pre-embedded transverse reinforcing rib fixing rod | |
| CN113201483B (en) | Non-invasive remote regulation and control method for regulating and controlling cell phenotype by virtue of cooperation of heterojunction and static magnetic field | |
| CN206599574U (en) | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time | |
| CN201261787Y (en) | Cell cultivation apparatus for exerting mechanical stimulation on cell | |
| Poulin et al. | Toward compression of small cell population: Harnessing stress in passive regions of dielectric elastomer actuators |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |