CN200999244Y - Cell substrate uniaxial drawing apparatus - Google Patents
Cell substrate uniaxial drawing apparatus Download PDFInfo
- Publication number
- CN200999244Y CN200999244Y CN 200720123317 CN200720123317U CN200999244Y CN 200999244 Y CN200999244 Y CN 200999244Y CN 200720123317 CN200720123317 CN 200720123317 CN 200720123317 U CN200720123317 U CN 200720123317U CN 200999244 Y CN200999244 Y CN 200999244Y
- Authority
- CN
- China
- Prior art keywords
- support
- cell
- line slideway
- pulling
- guide groove
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Mechanical Engineering (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Advancing Webs (AREA)
Abstract
A cell base uniaxial drawing device is provided, which comprises a step motor 3, a base 10, a bottom board 7, a linear bearing (19), a linear guide rail 4, a rectangular guide groove 8, a deflection module 20, a rectangular fixture 1, a pressure plate 2, a left parallel arm 17, a right parallel arm 18 and a motor bracket 6. The device can draw and load the cell and imitate that the cell adhered on the pellosil can generate the corresponding pulling and deforming under the action of the human inner stress. The uniaxial loading device pulling the strain makes the cell adhered on the silica gel basement membrane stretch passively mainly through pulling the culture basement membrane. Applying the cell base uniaxial drawing device can better imitate the mechanical environment of the tissue in human body when pulling and give pulling to the stillness, periodicity and the single axle of the cell. The strength of the pull force and the time frequency can be set. The device has simple and convenient application and good repeatability.
Description
Technical field
The present invention relates to a kind of static or periodic tensile loading device that is used for the single-axis bidirectional of cell cultures.
Background technology
Grinding cell cultures on chamber or the clinical medicine in fact, the cyto-mechanics characteristic, the research cell cultures is on flexible silicon glued membrane or other flexible materialss, cultivation there is the load that imposes of the pellosil of cell make it that corresponding strain take place, and this strain controlled, can realize the pair cell loading that stretches accordingly.Static or the periodic tensile loading device of single-axis bidirectional is also arranged aspect cell cultures at present, and the basilar membrane area is less can not realize big area cell cultures and shortcomings such as loading basilar membrane and sterilization inconvenience but exist.
Summary of the invention
Existing basilar membrane area is less can not realize big area cell cultures and deficiencies such as loading basilar membrane and sterilization inconvenience in order to overcome, the utility model provides a kind of cell tensile device that the big area cell cultures stretches and can carry out controls such as size, direction, frequency, time that can controlling strain that is suitable for, this device can not only produce and close the effects of action of symbol simulation in the human body internal stress, and has a frequency adjustment tractive flexibly, traction force size and time frequency can be set, and also have characteristics such as loading and sterilization convenience simultaneously.
Cell based bill kept on file axle stretching device involved in the present invention, on stepper-motor 3, support 10, base plate 7, linear bearings (19), line slideway 4, rectangle guide groove 8, yoke assembly 20, rectangular jig 1, pressing plate 2 left parallel arms 17, right parallel arms 18, electric machine support 6 are formed; Support 10 is installed on the base plate 7, four linear bearingss 19 are housed on the support 10, linear bearings 19 parallel being installed on the support 10, line slideway 4 is installed in the linear bearings 19, on two line slideways 4 rectangle guide groove 8 is housed, rectangle guide groove 8 is connected with yoke assembly 20, yoke assembly 20 rotates thereupon when stepper-motor 3 rotates, rectangle guide groove 8 is done parallel moving with line slideway 4, adjusting yoke assembly 20 can change the miles of relative movement with line slideway 4, line slideway 4 the other ends are equipped with rectangular jig 1, pressing plate 2 A/Fs of regulating on the rectangular jig 1 can the different basilar membrane of clamping, left parallel arms 17 and right parallel arms 18 are housed in the both sides of support 10, the parallel arms the other end is installed rectangular jig 1, and the anchor clamps keeping parallelism on these anchor clamps and the line slideway 4, stepper-motor 3 are installed on the electric machine support 6, electric machine support 6 is installed in support 10 tops, and stepper-motor 3 links to each other with controller.
The invention has the beneficial effects as follows: the basilar membrane length in anchor clamps that is loaded with cell can be regulated the basilar membrane that the different length thickness that vary in size have been satisfied in this design arbitrarily.Owing to adopted computer software control and pressure transmitter displacement sensor can regulate the stressed size of stretching frequency easily, this device preferably analogue body inner tissue be subjected to tractive the time mechanical environment etc.
Description of drawings
Fig. 1 is the front view of cell based bill kept on file axle stretching device
Fig. 2 is the left view of cell based bill kept on file axle stretching device
Fig. 3 is the vertical view of cell based bill kept on file axle stretching device
Fig. 4 is the deflection mechanism front view
Fig. 5 is the deflection mechanism vertical view
Embodiment
The present invention is further described as follows below in conjunction with accompanying drawing: cell based bill kept on file axle stretching device involved in the present invention directly drives stepper-motor by computer panel output pulse signal after the programming in the controller and by closed-loop control force transducer, displacement transducer signal is fed back to switchboard, main controlled variable is imported by computer, and the form with the printed report list after experiment is finished is exported experimental result.
From Fig. 2, as can be seen, support 10 is installed on the base plate 7, four linear bearingss 19 are housed on the support 10, linear bearings 19 parallel being installed on the support 10, line slideway 4 is installed in the linear bearings 19, on two line slideways 4 rectangle guide groove 8 is housed, rectangle guide groove 8 is connected with yoke assembly 20, yoke assembly 20 rotates thereupon when stepper-motor 3 rotates, rectangle guide groove 8 is done parallel moving with line slideway 4, regulate yoke assembly 20 and can change miles of relative movement with line slideway 4, line slideway 4 the other ends are equipped with rectangular jig 1, and pressing plate 2 A/Fs of regulating on the rectangular jig 1 can the different basilar membrane of clamping, left parallel arms 17 and right parallel arms 18 are housed in the both sides of support 10, and the parallel arms the other end is installed the anchor clamps keeping parallelism on rectangular jig 1 these anchor clamps and the line slideway 4.Stepper-motor 3 is contained on the electric machine support 6, is installed in support 10 tops at electric machine support 6, and stepper-motor 3 links to each other with controller.
Fig. 4 is yoke assembly 20 structure iron, wherein slide block 20-6 is installed among the deflection seat 20-4, offset distance adjustment axle 20-8 packs between slide block 20-6 and the deflection seat 20-4, on the axle 20-7 bearing 20-9 is housed, nut 20-2 locking bearing 20-9, axle 20-7 is contained among the slide block 20-6, reaches adjustment eccentricity purpose thereby rotation offset distance adjustment axle 20-8 can change slide block 20-6 position.
Claims (1)
1. cell based bill kept on file axle stretching device, by stepper-motor (3), support (10), on the base plate (7), linear bearings (19), line slideway (4), rectangle guide groove (8), yoke assembly (20), rectangular jig (1), the left parallel arms of pressing plate (2) (17), right parallel arms (18), electric machine support (6) is formed, it is characterized in that support (10) is installed on the base plate (7), four linear bearingss (19) are housed on the support (10), what linear bearings (19) was parallel is installed on the support (10), line slideway (4) is installed in the linear bearings (19), rectangle guide groove (8) is housed on two line slideways (4), rectangle guide groove (8) is connected with yoke assembly (20), yoke assembly (20) rotates thereupon when stepper-motor (3) rotates, rectangle guide groove (8) is done parallel moving with line slideway (4), adjusting yoke assembly (20) can change the miles of relative movement with line slideway (4), line slideway (4) the other end is equipped with rectangular jig (1), regulate the basilar membrane that pressing plate (2) A/F on the rectangular jig (1) can the clamping different thickness, left parallel arms (17) and right parallel arms (18) are housed in the both sides of support (10), the parallel arms the other end is installed rectangular jig (1), anchor clamps keeping parallelism on these anchor clamps and the line slideway (4), stepper-motor (3) is installed on the electric machine support (6), electric machine support (6) is installed in support (10) top, and stepper-motor (3) links to each other with controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720123317 CN200999244Y (en) | 2007-01-09 | 2007-01-09 | Cell substrate uniaxial drawing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200720123317 CN200999244Y (en) | 2007-01-09 | 2007-01-09 | Cell substrate uniaxial drawing apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN200999244Y true CN200999244Y (en) | 2008-01-02 |
Family
ID=39013649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200720123317 Expired - Fee Related CN200999244Y (en) | 2007-01-09 | 2007-01-09 | Cell substrate uniaxial drawing apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN200999244Y (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475908B (en) * | 2009-01-16 | 2011-06-29 | 中国人民解放军第三军医大学 | Cultivation apparatus and method for implementing continuous tension stress action to cell |
| CN101649291B (en) * | 2009-08-25 | 2011-12-21 | 四川大学 | Extension and compression device of multi-unit cells |
| CN103184144A (en) * | 2013-03-22 | 2013-07-03 | 中国科学院力学研究所 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
| CN103484367A (en) * | 2013-09-27 | 2014-01-01 | 中国人民解放军第三军医大学第一附属医院 | Uniaxial in-vitro cell drafting device capable of being cooled |
| CN103550016A (en) * | 2013-11-11 | 2014-02-05 | 中国人民解放军第三军医大学第一附属医院 | Uniform force applying device for bionic culturing of intervertebral disc |
| CN103555561A (en) * | 2013-10-29 | 2014-02-05 | 王卫明 | Biomechanical generator |
| CN105482996A (en) * | 2016-01-06 | 2016-04-13 | 西北工业大学 | Mechanical stimulation loading device for three-dimensional cell culture support |
| CN105670930A (en) * | 2016-03-25 | 2016-06-15 | 刘洪� | Controllable-pressure bioreactor for bio-printed cartilage |
| CN106867888A (en) * | 2017-03-20 | 2017-06-20 | 北京理工大学 | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time |
| CN107271265A (en) * | 2017-07-20 | 2017-10-20 | 浙江师范大学 | The biaxial stretch-formed device of dielectric elastomer list |
| CN110577895A (en) * | 2019-10-14 | 2019-12-17 | 东华大学 | dynamic cell culture method and culture device for simulating in-vivo dynamic environment |
-
2007
- 2007-01-09 CN CN 200720123317 patent/CN200999244Y/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475908B (en) * | 2009-01-16 | 2011-06-29 | 中国人民解放军第三军医大学 | Cultivation apparatus and method for implementing continuous tension stress action to cell |
| CN101649291B (en) * | 2009-08-25 | 2011-12-21 | 四川大学 | Extension and compression device of multi-unit cells |
| CN103184144A (en) * | 2013-03-22 | 2013-07-03 | 中国科学院力学研究所 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
| CN103484367A (en) * | 2013-09-27 | 2014-01-01 | 中国人民解放军第三军医大学第一附属医院 | Uniaxial in-vitro cell drafting device capable of being cooled |
| CN103484367B (en) * | 2013-09-27 | 2016-03-23 | 中国人民解放军第三军医大学第一附属医院 | The uniaxial in-vitro cell drafting assembly that can lower the temperature |
| CN103555561B (en) * | 2013-10-29 | 2015-06-03 | 王卫明 | Biomechanical generator |
| CN103555561A (en) * | 2013-10-29 | 2014-02-05 | 王卫明 | Biomechanical generator |
| CN103550016B (en) * | 2013-11-11 | 2015-05-27 | 中国人民解放军第三军医大学第一附属医院 | Uniform force applying device for bionic culturing of intervertebral disc |
| CN103550016A (en) * | 2013-11-11 | 2014-02-05 | 中国人民解放军第三军医大学第一附属医院 | Uniform force applying device for bionic culturing of intervertebral disc |
| CN105482996A (en) * | 2016-01-06 | 2016-04-13 | 西北工业大学 | Mechanical stimulation loading device for three-dimensional cell culture support |
| CN105482996B (en) * | 2016-01-06 | 2018-01-30 | 西北工业大学 | Three-dimensional cell culture support mechanical stimulation loading device |
| CN105670930A (en) * | 2016-03-25 | 2016-06-15 | 刘洪� | Controllable-pressure bioreactor for bio-printed cartilage |
| CN106867888A (en) * | 2017-03-20 | 2017-06-20 | 北京理工大学 | The at the uniform velocity single axis of symmetry tensile cell mechanics device that can be observed in place in real time |
| CN107271265A (en) * | 2017-07-20 | 2017-10-20 | 浙江师范大学 | The biaxial stretch-formed device of dielectric elastomer list |
| CN110577895A (en) * | 2019-10-14 | 2019-12-17 | 东华大学 | dynamic cell culture method and culture device for simulating in-vivo dynamic environment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN200999244Y (en) | Cell substrate uniaxial drawing apparatus | |
| CN1932510B (en) | Cell tensile loader | |
| CN102879269B (en) | Multifunctional stretcher capable of being loaded in multiple axial directions | |
| CN103845184A (en) | Rope-driven exoskeleton type upper-limb rehabilitation robot system | |
| CN205352857U (en) | Servo horizontal pressure testing machine that draws of electricity liquid | |
| CN101221166B (en) | Cell strain loading device under three-dimensional cultivation condition | |
| CN1932511A (en) | Sinusoidal tensile cell loader | |
| ITTO20080426A1 (en) | BIOREACTOR, IN PARTICULAR FOR THE GENERATION OF ENGINEERED FABRICS | |
| CN101397539B (en) | Force application apparatus simulating human physiological stress of tissue bionic culture for tissue engineering | |
| CN212426068U (en) | Tumor cell culture device for anti-tumor experiments | |
| CN205808825U (en) | A kind of electronics tensile and compression testing machine | |
| CN101906379A (en) | Device for precisely stretching visual cells under simulated in vivo environment | |
| CN110833507B (en) | Adjustable rat click device | |
| CN100570363C (en) | Cell mechanical period loading system | |
| CN103202734A (en) | Friction test experiment table of orthodontic appliance | |
| CN201737929U (en) | Precision visualization cell stretching device under environment simulating inner environment of human body | |
| CN205080009U (en) | 3 fatigue bending machine | |
| CN201660554U (en) | Beam-splitting plate lifting mechanism of wire-drawing machine of glass fiber yarn cake | |
| CN201149589Y (en) | Large displacement cell strain loading device under three-dimensional cultivation condition | |
| CN201149590Y (en) | Large active force cell strain loading device under three-dimensional cultivation condition | |
| CN201390007Y (en) | Laminator | |
| CN203269948U (en) | Device for tendon and tendon sheath in vivo co-culture construction | |
| CN205439056U (en) | Complete electronic injection device of two motor drive formulas of mariages thick stick | |
| Kurata et al. | Open-source cell extension system assembled from laser-cut plates | |
| CN201825957U (en) | Bioreactor with lift-adjustable double-frequency loading device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080102 Termination date: 20100209 |