CN103555561A - Biomechanical generator - Google Patents

Biomechanical generator Download PDF

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Publication number
CN103555561A
CN103555561A CN201310524719.0A CN201310524719A CN103555561A CN 103555561 A CN103555561 A CN 103555561A CN 201310524719 A CN201310524719 A CN 201310524719A CN 103555561 A CN103555561 A CN 103555561A
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China
Prior art keywords
main body
gear
crank
threaded rod
base
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CN201310524719.0A
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CN103555561B (en
Inventor
王卫明
万朝燕
陈秉智
向先祥
赵东洋
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli

Abstract

The invention discloses a biochemical generator, which comprises a base, a dovetail guide sliding rail, a workbench main body and a biomechanical generation mechanism, wherein the dovetail guide sliding rail is perpendicular to the upper end face of the base; the workbench main body is matched with the dovetail guide sliding rail through a dovetail groove; the biomechanical generation mechanism is arranged on the upper end face of a workbench; a threaded rod is perpendicularly arranged on the upper end face of the base; a gear mechanism for driving the threaded rod to rotate is arranged in the base; a worm gear is arranged on the lower end face of the workbench main body, and is assembled with the workbench main body in a rotating way; a worm for driving the worm gear to rotate is arranged on the workbench main body; the worm gear is sleeved on the threaded rod, and is in threaded fit with the threaded rod. According to the biochemical generator, the workbench is quickly lifted through a rough adjustment device (the gear mechanism), and is lifted by a minor distance through a fine adjustment device (a worm gear and worm matching mechanism), and a crank-link mechanism is driven by a motor to drive a simulated mechanical contact of the biochemical generation mechanism to reciprocally rotate, so that virtual shear force is generated on the surface of a biological membrane on the contact by the swinging of the biological membrane.

Description

A kind of biomechanics producer
Technical field
The present invention relates to a kind of biomechanics producer.
Background technology
Development along with medical science, especially the development of osteology, mixing together between itself and biomechanics is more and more tightr, in orthopaedics field, the regression of backbone and intervertebral disk thereof is a kind of common disease, but at present the direction of its research is mainly concentrated on and on living animal, gives the model that mechanical stimulation causes backbone and intervertebral disc degeneration, then pass through cytology, molecular biology, the mechanism of regression is studied in immunology detection and analysis, such research is static research in the cytology stage, the cell of stressed whole last current state to be carried out to the analysis of form and cytoskeleton, do not have at present device can simulate dynamic stress and the compound stress of normal human's backbone and intervertebral disk completely, and then cause the general form that can not occur at dynamic lower observation of cell under continuous mechanical stimulation effect, microtexture, cytoskeleton, the variation that biology Transport occurs, just cannot at dynamic stress and compound stress, carry out observational study to terminal plate of vertebral body chondrocyte.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of biomechanics producer; to normal active somatic cell, provide dynamic mechanical stimulation; this biomechanics producer not only can continue to give mechanical stimulation; and can change within the specific limits application of force direction, iterative motion; structural strength is good; easily manufactured, cost is lower.The more important thing is and can in cell culture incubator environment, operate.
For reaching above object, be achieved through the following technical solutions:
A producer, comprising: base, and the dovetail directive slide track that vertical base upper surface arranges, coordinates the worktable main body of assembling and the biomechanics generating mechanism that is arranged at worktable upper surface with dovetail directive slide track by dovetail-indent;
Base upper surface is vertically installed with threaded rod, and threaded rod lower end is inserted into base inside; Base inside is provided with for driving the gear mechanism of threaded rod rotation, and gear mechanism at least comprises two-stage gear;
Worktable main body lower surface is provided with turbine, and turbine and worktable main body be for rotating assembling, and turbine can circumferentially freely rotate with respect to worktable main body; In worktable main body, be provided with for driving the scroll bar of turbine rotation;
Cover For A Turbine is placed on threaded rod, and with threaded rod thread fit;
Worktable main body is positioned at threaded rod central axis bearing of trend and is provided with the through hole stretching out for threaded rod;
Biomechanics generating mechanism comprises: simulation mechanics contact and toggle; Toggle comprises, the first power crank, the second crank, the 3rd wobble crank and external tooth cover;
Worktable body interior is provided with power motor, and power motor output shaft and the first power crank are connected and fixed; Second crank one end is connected with the other end of the first power crank by rotating shaft, and the 3rd wobble crank one end is connected with the other end of the second crank by rotating shaft, and the 3rd wobble crank is fixed with external tooth cover; External tooth cover is arranged at worktable main body upper surface by rotating shaft, and external tooth cover central axis is vertical with worktable main body, and external tooth cover can it freely rotate around central axis;
Simulation mechanics contact is 4, be vertically installed in worktable main body upper surface, and each simulation mechanics contact is arranged at worktable main body upper surface by rotating shaft, and each simulation mechanics contact center axis is vertical with worktable main body upper surface, and each simulation mechanics contact can freely rotate around its central axis; On each simulation mechanics contact, be provided with and external tooth cover meshed gears, and each gear is identical;
Scroll bar stretches out in worktable bulk end and is fixed with handwheel;
In the first power crank, be provided with at least 4 gear adjustment holes of installing for the second crank;
Gear mechanism comprises: the driving gear of power input, for drive the power gear of threaded rod rotation and be assemblied in driving gear and power gear and respectively with the transition gear of above-mentioned two gears engagement; Driving gear, power gear and transition gear are all assemblied in base inside by rotating shaft, and base upper end is provided with for driving the regulating wheel of driving gear rotation;
In regulating wheel, be arranged at detent, and base upper end is provided with the locking lever mechanism that coordinates location with detent;
Adopt the present invention of technique scheme, first adjust worktable main body with respect to the position of base, user rotates regulating wheel, and then drive driving gear, (quantity of transition gear is not limit herein in the gear unit rotation that transition gear and power gear form, can realize base overall volume by increase transition gear reduces), power gear drives threaded rod to rotate, because turbine and threaded rod are thread fit, and then turbine drives worktable main body, along dovetail directive slide track, rise or decline, then by locking lever mechanism, (base upper end can be fixed on by hinge in locking lever mechanism herein motion bar or latch structure are realized, as select motion bar the groove in regulating wheel rotation process motion bar and regulating wheel depart from, when needs are located regulating wheel, only motion bar need to be inserted in the groove in regulating wheel and can meet location needs, when adopting latch, in susceptor surface, need to process the hole of inserting location for latch, latch when regulating wheel need to be located is as long as directly can meet location through corresponding pilot hole on the groove in regulating wheel or through hole inserted base.) locate the position that regulating wheel is taken turns, make preliminary location positioning, configuration requirement is herein: regulating wheel turns around, threaded rod turns 5.536 circles, worktable rising 8.304mm;
Then by rotating handwheel, drive scroll bar to rotate, and then drive turbine rotation, because threaded rod now is located and do not rotated, the independent rotation of turbine can complete the adjustment of further position, configuration requirement is herein: can make worktable rising 0.01829mm, microspur rises, and guarantees high precision;
After worktable body position is determined, start power motor, power motor drives the first power crank full circle revolution, drive the second crank to rotate, drive the 3rd wobble crank, because the 3rd wobble crank and external tooth cover are fixed, and external tooth cover is fixed by rotating shaft and worktable main body upper surface, and then the 3rd wobble crank can to do with respect to external tooth cover rotating shaft and power motor power output shaft line be symmetric line swings back and forth, and then external tooth cover completes the rotation of reciprocal certain angle, and then by gear driven, simulate mechanics contact and rotate, and then complete the virtual of biomechanics, configuration requirement is herein: the 3rd wobble crank two place's maximum position angle scopes, by the second crank, being arranged on which assembling adjustment hole of the first power crank determines, as preferred 4 gears, completing respectively the 3rd wobble crank two place's maximum position angle scopes is 3.75 °, 7.5 °, 11.25 ° and 15 °, to adapt to human body high frequency backbone rotating range by a narrow margin.
To sum up:
As preferably, described worktable is made by stainless steel, to adapt to incubator humidity, gas concentration lwevel.
As preferably, described simulation mechanics contact, by contacting with culture dish microbial film, circulation rotating, thus twisting microbial film makes the cell of adherent growth in liquid environment, suffer liquid and biomembranous double direction shear power.
Use when of the present invention, healthy passage cell need be incubated to bottom in biomembranous culture dish, culture dish is placed on worktable, by rack and rinion adjustment (gear mechanism), make worktable fast rise, then by micromatic setting (turbine and scroll bar matching mechanism), making to simulate mechanics contact contacts with microbial film closely, to guarantee that the microbial film that rotarily drives of simulation mechanics contact twists to produce shearing force on its surface, the power-equipment of this contrive equipment adopts driven by motor, this motor can be worked 500 hours, can guarantee to test to carry out smoothly.
The invention has the advantages that the rotational shear power that simulation human intervertebral disc bears when normal activity; dynamically observe the variation of intervertebral disk endplate cartilage cell form, microtexture and transfer ability under particular organisms mechanical environment, effect and mechanism of action that research particular organisms mechanical state is brought into play in intervertebral disc degeneration process.
Above-mentioned explanation is only the general introduction of technical solution of the present invention, in order to better understand technique means of the present invention, and can be implemented according to the content of specification sheets, and for above and other object of the present invention, feature and advantage can be become apparent, below especially exemplified by preferred embodiment, and coordinate accompanying drawing, be described in detail as follows.
Accompanying drawing explanation
The present invention is totally 4 width accompanying drawings, wherein:
Fig. 1 is side-looking structural representation of the present invention.
Fig. 2 is plan structure schematic diagram of the present invention.
Fig. 3 is the perspective structure schematic diagram of worktable main body of the present invention.
Fig. 4 is the cross-sectional view of base of the present invention.
In figure: 1, base, 2, dovetail directive slide track, 3, worktable main body, 4, biomechanics generating mechanism, 5, threaded rod, 6, gear mechanism, 7, turbine, 8, scroll bar, 9, simulation mechanics contact, 10, toggle, the 11, first power crank, the 12, second crank, 13, the 3rd wobble crank, 14, external tooth cover, 15, handwheel, 16, assembling adjustment hole, 17, driving gear, 18, power gear, 19, transition gear, 20, regulating wheel ,21, locking lever mechanism.
Embodiment
A kind of biomechanics producer as shown in Figure 1, Figure 2, Figure 3 and Figure 4, comprise: base 1, the dovetail directive slide track 2 that vertical base 1 upper surface arranges, coordinates the worktable main body 3 of assembling and the biomechanics generating mechanism 4 that is arranged at worktable upper surface with dovetail directive slide track 2 by dovetail-indent;
Base 1 upper surface is vertically installed with threaded rod 5, and threaded rod 5 lower ends are inserted into base 1 inside; Base 1 inside is provided with for driving the gear mechanism 6 of threaded rod 5 rotations, and gear mechanism 6 at least comprises two-stage gear;
Worktable main body 3 lower surfaces are provided with turbine 7, and turbine 7 with worktable main body 3 for rotating assembling, turbine 7 can circumferentially freely rotate with respect to worktable main body 3; In worktable main body 3, be provided with for driving the scroll bar 8 of turbine 7 rotations;
Turbine 7 is placed on threaded rod 5, and with threaded rod 5 thread fit;
Worktable main body 3 is positioned at threaded rod 5 central axis bearing of trends and is provided with the through hole stretching out for threaded rod 5;
Biomechanics generating mechanism 4 comprises: simulation mechanics contact 9 and toggle 10; Toggle 10 comprises, the first power crank 11, the second crank 12, the 3rd wobble crank 13 and external tooth cover 14;
Worktable main body 3 inside are provided with power motor, and power motor output shaft and the first power crank 11 are connected and fixed; Second crank 12 one end are connected with the other end of the first power crank 11 by rotating shaft, and the 3rd wobble crank 13 one end are connected with the other end of the second crank 12 by rotating shaft, and the 3rd wobble crank 13 is fixed with external tooth cover 14; External tooth cover 14 is arranged at worktable main body 3 upper surfaces by rotating shaft, and external tooth cover 14 central axis are vertical with worktable main body 3, and external tooth cover 14 can it freely rotate around central axis;
Simulation mechanics contact 9 is 4, be vertically installed in worktable main body 3 upper surfaces, and each simulation mechanics contact 9 is arranged at worktable main body 3 upper surfaces by rotating shaft, and each simulation mechanics contact 9 central axis is vertical with worktable main body 3 upper surfaces, and each simulation mechanics contact 9 can freely rotate around its central axis; On each simulation mechanics contact 9, be provided with and external tooth cover 14 meshed gears, and each gear is identical;
Scroll bar 8 stretches out in worktable main body 3 ends and is fixed with handwheel 15;
In the first power crank 11, be provided with at least 4 assembling adjustment holes 16 of installing for the second crank 12;
Gear mechanism 6 comprises: the driving gear 17 of power input, for drive the power gear 18 of threaded rod 5 rotations and be assemblied in driving gear 17 and power gear 18 and respectively with the transition gear 19 of above-mentioned two gears engagement; Driving gear 17, power gear 18 and transition gear 19 are all assemblied in base 1 inside by rotating shaft, and base 1 upper end is provided with for driving the regulating wheel 20 of driving gear 17 rotations;
In regulating wheel 20, be arranged at detent, and base 1 upper end is provided with the locking lever mechanism 21 that coordinates location with detent;
Adopt the present invention of technique scheme, first adjust worktable main body 3 with respect to the position of base 1, user rotates regulating wheel 20, and then drive driving gear 17, (quantity of transition gear is not limit herein in the gear unit rotation that transition gear 19 and power gear 18 form, can realize base overall volume by increase transition gear reduces), power gear 18 drives threaded rod 5 to rotate, because turbine 7 is thread fit with threaded rod 5, and then turbine 7 drives worktable main body 3, along dovetail directive slide track 2, rise or decline, then by the 21(of locking lever mechanism base upper end can be fixed on by hinge in locking lever mechanism motion bar or latch structure herein, realize, as select motion bar the groove in regulating wheel rotation process motion bar and regulating wheel depart from, when needs are located regulating wheel, only motion bar need to be inserted in the groove in regulating wheel and can meet location needs, when adopting latch, in susceptor surface, need to process the hole of inserting location for latch, latch when regulating wheel need to be located is as long as directly can meet location through corresponding pilot hole on the groove in regulating wheel or through hole inserted base, lock-out state as shown in Figure 4, released state as shown in Figure 1.) locate the position that regulating wheel 20 is taken turns, make preliminary location positioning, configuration requirement is herein: regulating wheel 20 turns around, threaded rod 5 turns 5.536 circles, worktable rising 8.304mm;
Then by rotating handwheel 15, drive scroll bar 8 to rotate, and then drive turbine 7 to rotate, because threaded rod 5 now has been located and do not rotated, the independent rotation of turbine 7 can complete the adjustment of further position, configuration requirement is herein: can make worktable rising 0.01829mm, microspur rises, and guarantees high precision;
After worktable main body 3 location positionings, start power motor, power motor drives the first power crank 11 full circle revolution, drive the second crank 12 to rotate, drive the 3rd wobble crank 13, because the 3rd wobble crank 13 is fixing with external tooth cover 14, and external tooth cover 14 is fixed by rotating shaft and worktable main body 3 upper surfaces, and then the 3rd wobble crank 13 can to do with respect to external tooth cover 14 rotating shafts and power motor power output shaft line be symmetric line swings back and forth, and then external tooth cover 14 completes the rotation of reciprocal certain angle, and then by gear driven, simulate mechanics contact 9 and rotate, and then complete the virtual of biomechanics, configuration requirement is herein: the 3rd 13 liang of wobble cranks are located maximum position angle scope, by the second crank 12, being arranged on first power crank 11 which assembling adjustment hole 16 determines, as preferred 4 gears, completing respectively 13 liang of the 3rd wobble cranks, to locate maximum position angle scope be 3.75 °, 7.5 °, 11.25 ° and 15 °, to adapt to human body high frequency backbone rotating range by a narrow margin.
To sum up:
As preferably, described worktable is made by stainless steel, to adapt to incubator humidity, gas concentration lwevel.
As preferably, described simulation mechanics contact, by contacting with culture dish microbial film, circulation rotating, thus twisting microbial film makes the cell of adherent growth in liquid environment, suffer liquid and biomembranous double direction shear power.
Use when of the present invention, healthy passage cell need be incubated to bottom in biomembranous culture dish, culture dish is placed on worktable, by rack and rinion adjustment, make worktable fast rise, then by micromatic setting, make to simulate mechanics contact and contact with microbial film closely, to guarantee that the microbial film that rotarily drives of simulating mechanics contact twists to produce shearing force on its surface, the power-equipment of this contrive equipment adopts driven by motor, this motor can be worked 500 hours, can guarantee to test to carry out smoothly.
The invention has the advantages that the rotational shear power that simulation human intervertebral disc bears when normal activity; dynamically observe the variation of intervertebral disk endplate cartilage cell form, microtexture and transfer ability under particular organisms mechanical environment, effect and mechanism of action that research particular organisms mechanical state is brought into play in intervertebral disc degeneration process.
The above, it is only preferred embodiment of the present invention, not the present invention is done to any pro forma restriction, although the present invention discloses as above with preferred embodiment, yet not in order to limit the present invention, any those skilled in the art are not departing within the scope of technical solution of the present invention, when utilizing, appeal the equivalent embodiment that the technology contents disclosing is made a little change or is modified to equivalent variations, in every case be the content that does not depart from technical solution of the present invention, foundation technical spirit of the present invention is to any simple modification made for any of the above embodiments, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.

Claims (5)

1. a biomechanics producer, it is characterized in that: comprise, base (1), the dovetail directive slide track (2) that vertical base (1) upper surface arranges, coordinates the worktable main body (3) of assembling and is arranged at the biomechanics generating mechanism (4) of worktable upper surface with dovetail directive slide track (2) by dovetail-indent;
Described base (1) upper surface is vertically installed with threaded rod (5), and threaded rod (5) lower end is inserted into base (1) inside; Described base (1) inside is provided with for driving the gear mechanism (6) of threaded rod (5) rotation, and gear mechanism (6) at least comprises two-stage gear;
Described worktable main body (3) lower surface is provided with turbine (7), and described turbine (7) and worktable main body (3) be for rotating assembling, and turbine (7) can be with respect to worktable main body (3) circumferential movement; In described worktable main body (3), be provided with for driving the scroll bar (8) of turbine (7) rotation;
It is upper that described turbine (7) is placed on threaded rod (5), and with threaded rod (5) thread fit;
Described worktable main body (3) is positioned at threaded rod (5) central axis bearing of trend and is provided with the through hole stretching out for threaded rod (5);
Described biomechanics generating mechanism (4) comprising: simulation mechanics contact (9) and toggle (10); Described toggle (10) comprises, the first power crank (11), the second crank (12), the 3rd wobble crank (13) and external tooth cover (14);
Described worktable main body (3) inside is provided with power motor, and power motor output shaft and the first power crank (11) are connected and fixed; Described the second crank (12) one end is connected with the other end of the first power crank (11) by rotating shaft, the 3rd wobble crank (13) one end is connected with the other end of the second crank (12) by rotating shaft, and the 3rd wobble crank (13) is fixed with external tooth cover (14); Described external tooth cover (14) is arranged at worktable main body (3) upper surface by rotating shaft, and external tooth cover (14) central axis is vertical with worktable main body (3), and external tooth cover (14) can it freely rotate around central axis;
Described simulation mechanics contact (9) is 4, be vertically installed in worktable main body (3) upper surface, and each simulation mechanics contact (9) is arranged at worktable main body (3) upper surface by rotating shaft, and each simulation mechanics contact (9) central axis is vertical with worktable main body (3) upper surface, and each simulation mechanics contact (9) can freely rotate around its central axis; On each simulation mechanics contact (9), be provided with and external tooth cover (14) meshed gears, and each gear is identical.
2. a kind of biomechanics producer according to claim 1, is characterized in that: described scroll bar (8) stretches out in worktable main body (3) end and is fixed with handwheel (15).
3. a kind of biomechanics producer according to claim 1, is characterized in that: in described the first power crank (11), be provided with at least 4 gear adjustment holes (16) of installing for the second crank (12).
4. a kind of biomechanics producer according to claim 1, it is characterized in that: described gear mechanism (6) comprising: the driving gear (17) of power input, for driving the power gear (18) of threaded rod (5) rotation and being assemblied in driving gear (17) and power gear (18) and the transition gear (19) that meshes with above-mentioned two gears respectively; Described driving gear (17), power gear (18) and transition gear (19) are all assemblied in base (1) inside by rotating shaft, and described base (1) upper end is provided with for driving the regulating wheel (20) of driving gear (17) rotation.
5. a kind of biomechanics producer according to claim 4, is characterized in that: described regulating wheel is arranged at detent on (20), and base (1) upper end is provided with the locking lever mechanism (21) that coordinates location with detent.
CN201310524719.0A 2013-10-29 2013-10-29 Biomechanical generator Expired - Fee Related CN103555561B (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104785886A (en) * 2015-04-29 2015-07-22 武汉一冶钢结构有限责任公司 Inclined hole cutting assembly and method for machining inclined holes through same
CN106680114A (en) * 2017-01-19 2017-05-17 苏州大学 Soft tissue anisotropy shearing mechanical property tester
CN112603285A (en) * 2020-12-23 2021-04-06 中科彭州智慧产业创新中心有限公司 Controllable mechanics generator
CN112608845A (en) * 2021-01-11 2021-04-06 兰州大学第一医院 Bionic stimulation device for mechanical pressure of stem cells
CN115372149A (en) * 2022-10-24 2022-11-22 中国中医科学院望京医院(中国中医科学院骨伤科研究所) Circumferential loading device, method and loading system for spine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN200999244Y (en) * 2007-01-09 2008-01-02 重庆大学 Cell substrate uniaxial drawing apparatus
CN203513666U (en) * 2013-10-29 2014-04-02 王卫明 Biomechanics generator

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN200999244Y (en) * 2007-01-09 2008-01-02 重庆大学 Cell substrate uniaxial drawing apparatus
CN203513666U (en) * 2013-10-29 2014-04-02 王卫明 Biomechanics generator

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104785886A (en) * 2015-04-29 2015-07-22 武汉一冶钢结构有限责任公司 Inclined hole cutting assembly and method for machining inclined holes through same
CN104785886B (en) * 2015-04-29 2018-06-12 武汉一冶钢结构有限责任公司 A kind of method of inclined hole cutting assembly and its processing inclined
CN106680114A (en) * 2017-01-19 2017-05-17 苏州大学 Soft tissue anisotropy shearing mechanical property tester
CN112603285A (en) * 2020-12-23 2021-04-06 中科彭州智慧产业创新中心有限公司 Controllable mechanics generator
CN112608845A (en) * 2021-01-11 2021-04-06 兰州大学第一医院 Bionic stimulation device for mechanical pressure of stem cells
CN112608845B (en) * 2021-01-11 2023-07-25 兰州大学第一医院 Stem cell mechanical pressure bionic stimulation device
CN115372149A (en) * 2022-10-24 2022-11-22 中国中医科学院望京医院(中国中医科学院骨伤科研究所) Circumferential loading device, method and loading system for spine
CN115372149B (en) * 2022-10-24 2023-01-31 中国中医科学院望京医院(中国中医科学院骨伤科研究所) Circumferential loading device, method and loading system for spine

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