CN101805694B - Gravity mixing bioreactor obliquely rotating along central shaft of culture vessel - Google Patents
Gravity mixing bioreactor obliquely rotating along central shaft of culture vessel Download PDFInfo
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- CN101805694B CN101805694B CN2010101025898A CN201010102589A CN101805694B CN 101805694 B CN101805694 B CN 101805694B CN 2010101025898 A CN2010101025898 A CN 2010101025898A CN 201010102589 A CN201010102589 A CN 201010102589A CN 101805694 B CN101805694 B CN 101805694B
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Abstract
The invention discloses a gravity mixing bioreactor obliquely rotating along a central shaft of a culture vessel, which is a bioreactor capable of causing a culture flask with cylindrical body to implement microcarrier cell culture. In a frame playing the role of fixation and support, an electric motor drives a plurality of cylindrical transmission shafts, which are parallel to each other and form an angle from 5 to 80 degrees in relation to horizontal plane, to rotate so as to drive the cylindrical culture flask to rotate. The cylindrical transmission shafts of the reactor are divided into active rotation transmission shafts and passive rotation transmission shafts adjacent thereto, two adjacent transmission shafts and support bases at the bottoms thereof jointly support the cylindrical culture vessel to rotate. Transmission in transmission belt or gear can be achieved between cylindrical active rotation transmission shafts in rows or columns as well as between cylindrical active rotation transmission shafts in rows or columns and the electric motor. The gravity mixing bioreactor can comprise a circuit control part and can be accommodated in a small temperature controller or placed in a temperature control room.
Description
Technical field
The microcarrier cell cultures is the main method that mass cell is cultivated; The conventional bio-reactor that is used for the microcarrier cell cultures costs an arm and a leg; Complex structure needs the operator of specialty to use, and the biological reaction apparatus that the present invention relates to is economical and practical; Simple to operate, make the microcarrier cell cultures equally easy as common cell bottle cell cultures.
Background technology
Because there are many, the easy contaminating microorganisms of big, the required bottle of labor capacity quantity in traditional rolling bottle culturing cell, power consumption is big and produce shortcomings such as dirt is many, the microcarrier cell culture technology receives publicity day by day.And the bio-reactor of import costs an arm and a leg, and the home-made bio-reactor is cheap, the bad again assurance of culture effect and stability.
Summary of the invention
In order to address the above problem, the invention provides a kind of microcarrier biological reaction apparatus can be that column culture vessel (like 3L rolling bottle etc.) carries out the microcarrier cell cultures through main body, reaches high-cell density, economical and practical.
The technical scheme that the present invention adopted is: a kind of main body that makes is the biological reaction apparatus that columned culturing bottle carries out the microcarrier cell cultures; In the framework that plays fixing and supporting role; Rotate to drive cylindric culturing bottle rotation by direct motor drive 1 row or multirow, 1 row or multiple row column transmission shaft, carry out the microcarrier cell cultures.
The column transmission shaft of this biological reaction apparatus can be divided into many groups; Each group be 1 by electric motor driven active rotation transmission shaft be adjacent about each passive rotation transmission shaft free to rotate forms, 2 adjacent transmission shafts are held the rotation of column culture vessel jointly with the base for supporting of its bottom.
Each column transmission shaft of this biological reaction apparatus, the column culture vessel is parallel to each other and and horizontal plane be 5～80 degree angles.
This biological reaction apparatus each the row or each row cylindrical active rotation transmission shaft between, each the row or each row cylindrical active rotation transmission shaft and phonomoter between can use transmission belt transmission or geartransmission.
This biological reaction apparatus can contain the pilot circuit part.
This biological reaction apparatus can be contained in the small-sized temperature control container, also can it directly be put in the temperature-controlled chamber.
Description of drawings
Fig. 1, Fig. 2 are the side-view of realizing two kinds of types of the design's thinking
2 groups of column transmission shafts that Fig. 3 links to each other and places for level
Fig. 4 is the schematic perspective view with the column transmission shaft group at horizontal plane journey 5～80 degree angles
(1) framework that play fixed supporting role; (2) phonomoter; (3) column transmission shaft group; (4) crossbeam; (5) belt or the gear of connection cylindrical active rotation transmission shaft; (6) belt or the gear of connection cylindrical active rotation transmission shaft and phonomoter; (7) position of the cylindric culturing bottle of placement; (8) cylindrical active rotation transmission shaft; (9) passive rotation column transmission shaft; (10) rubber jacket layer; (11) base for supporting of placement column culture vessel;
Embodiment
For making the feature and advantage of the present invention can be more obviously understandable, the present invention be done further detailed explanation below in conjunction with accompanying drawing and embodiment.
Fig. 1, Fig. 2 are the side-view of realizing two kinds of types of the design's thinking; Connecting on the crossbeam (4) in framework (1) by 1 row or multirow, 1 row or multiple row is parallel to each other and with the column transmission shaft group (3) at horizontal plane journey 5～80 degree angles; The cylindrical active rotation transmission shaft of the cylindric culturing bottle stationary installation of every row or every row (3) is connected by belt or gear (5); And be connected to common phonomoter (2) through belt or gear (6), rotate by pilot circuit control direct motor drive cylindrical active rotation transmission shaft.
Fig. 3 is the frontview of the column culture vessel stationary installation of horizontal positioned; 1 passive rotation column transmission shaft (9) is arranged in the adjacent both sides of its cylindrical active rotation transmission shaft (8); Each is walked crosswise can have a plurality of active rotation axles; The spacing of all adjacent rotated axles all equates, and and these two adjacent rotation axiss between base for supporting (10) form 1 position (7) of placing cylindric culturing bottle jointly.
Fig. 4 is and the schematic perspective view of the cylindric culturing bottle stationary installation stationary installation at horizontal plane journey 5～80 degree angles, is fixed with rubber jacket layer (11) on all rotation axiss.
Claims (4)
- One kind can to make main body be the biological reaction apparatus that columned culturing bottle carries out the microcarrier cell cultures; In the framework that plays fixing and supporting role; Rotate to drive cylindric culturing bottle rotation by direct motor drive multirow, multiple row column transmission shaft; Carry out the microcarrier cell cultures, each column transmission shaft, cylindric culturing bottle is parallel to each other and all tilt to be connected in the support frame; The column transmission shaft that tilts can be divided into many groups; Each group be 1 by electric motor driven cylindrical active rotation transmission shaft be adjacent about each passive rotation column transmission shaft free to rotate forms, 2 adjacent transmission shafts are held cylindric culturing bottle rotation jointly with the base for supporting of its bottom; Each column transmission shaft parallel to each other, column culturing bottle all and horizontal plane be 5～80 degree angles; Between each each row cylindrical active rotation transmission shaft of row, use the transmission belt transmission between each row each row cylindrical active rotation transmission shaft and phonomoter.
- 2. biological reaction apparatus according to claim 1 is characterized in that it contains the pilot circuit part, and pilot circuit comprises power switch, electromotor velocity regulating circuit, corresponding button.
- 3. according to any described biological reaction apparatus in the claim 1～2, it is characterized in that it is placed in the temperature control container.
- 4. according to claim 3 a described biological reaction apparatus, it is characterized in that temperature regulator comprises greenhouse, incubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101025898A CN101805694B (en) | 2010-01-29 | 2010-01-29 | Gravity mixing bioreactor obliquely rotating along central shaft of culture vessel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010101025898A CN101805694B (en) | 2010-01-29 | 2010-01-29 | Gravity mixing bioreactor obliquely rotating along central shaft of culture vessel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101805694A CN101805694A (en) | 2010-08-18 |
| CN101805694B true CN101805694B (en) | 2012-07-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010101025898A Active CN101805694B (en) | 2010-01-29 | 2010-01-29 | Gravity mixing bioreactor obliquely rotating along central shaft of culture vessel |
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| Country | Link |
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| CN (1) | CN101805694B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521946B (en) * | 2015-01-26 | 2016-08-17 | 深圳康泰生物制品股份有限公司 | Cell freezing apparatus and method |
| CN105548529A (en) * | 2016-01-16 | 2016-05-04 | 刘静 | Special test device for cell secreta |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546642A (en) * | 2003-12-10 | 2004-11-17 | 上海伯瑞生物技术发展有限公司 | Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture |
| CN1321175C (en) * | 2004-01-13 | 2007-06-13 | 中国科学院动物研究所 | Microcarrier for large-scale culture cell |
| CN200949106Y (en) * | 2006-09-22 | 2007-09-19 | 韩毅鹏 | Tilted two-level rotating cylinder bioreactor |
| CN100360657C (en) * | 2005-03-30 | 2008-01-09 | 韩毅鹏 | Horizontal drum type bioreactor |
| CN201634681U (en) * | 2010-01-29 | 2010-11-17 | 福尔生物制药股份有限公司 | Gravity hybrid biological reaction device slantwise rotating along central shaft of culture container |
-
2010
- 2010-01-29 CN CN2010101025898A patent/CN101805694B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546642A (en) * | 2003-12-10 | 2004-11-17 | 上海伯瑞生物技术发展有限公司 | Cell depositing interception device of hematopoietic cell continuous perfusion suspended culture |
| CN1321175C (en) * | 2004-01-13 | 2007-06-13 | 中国科学院动物研究所 | Microcarrier for large-scale culture cell |
| CN100360657C (en) * | 2005-03-30 | 2008-01-09 | 韩毅鹏 | Horizontal drum type bioreactor |
| CN200949106Y (en) * | 2006-09-22 | 2007-09-19 | 韩毅鹏 | Tilted two-level rotating cylinder bioreactor |
| CN201634681U (en) * | 2010-01-29 | 2010-11-17 | 福尔生物制药股份有限公司 | Gravity hybrid biological reaction device slantwise rotating along central shaft of culture container |
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| Publication number | Publication date |
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| CN101805694A (en) | 2010-08-18 |
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Inventor after: Yang Guosong Inventor after: Ji Guanghui Inventor before: Yang Guosong |
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Owner name: ZHONGKE BIOLOGICAL PHARMACEUTICAL CO., LTD. Free format text: FORMER NAME: FORWELL BIOPHARMACEUTICALS CO., LTD. |
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| CP03 | Change of name, title or address |
Address after: 065201 No. 1155 Development Zone, Sanhe, Hebei, Yanjiao Patentee after: ZHONGKE BIOPHARM CO.,LTD. Address before: 065201 Yingbin Road 38-2, Yanjiao Development Zone, Hebei, Sanhe Patentee before: FORWELL BIOPHARMACEUTICALS Co.,Ltd. |
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Address after: No. 1155, Yanjiao Development Zone, Sanhe City, Hebei Province, 065201 Patentee after: Bohui Biopharmaceutical Co.,Ltd. Address before: No. 1155, Yanjiao Development Zone, Sanhe City, Hebei Province, 065201 Patentee before: ZHONGKE BIOPHARM CO.,LTD. |
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