CN203559059U - Full-automatic three-dimensional cell culturing system - Google Patents
Full-automatic three-dimensional cell culturing system Download PDFInfo
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- CN203559059U CN203559059U CN201320470380.6U CN201320470380U CN203559059U CN 203559059 U CN203559059 U CN 203559059U CN 201320470380 U CN201320470380 U CN 201320470380U CN 203559059 U CN203559059 U CN 203559059U
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- pedestal
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Abstract
The utility model relates to a full-automatic three-dimensional cell culturing system. The system comprises a pedestal, a power adapter and culture dishes, wherein a rotary motor is mounted in the pedestal; a revolution meter is arranged on the power adapter; the culture dishes are culture discs or culture tube columns, and silica gel gas exchange films are arranged on back sides of the culture discs or the culture tube columns; the culture dishes are mounted on the pedestal; the pedestal is connected with the power adapter. According to the full-automatic three-dimensional cell culturing system, cells or tissues are in a free-falling suspension state in a growth process, and destructive pressures caused by a stirrer, bubbles and the like are avoided, so that the tissues can freely fall and overturn in a culture liquid and are adequately mixed with the culture liquid; forces in all directions in a container are balanced, so that the cells or the tissues cannot be influenced by the force in a single direction and can uniformly grow towards arbitrary direction, the cell proliferation velocity is increased, the cell death is reduced, the secretion of cell products is increased, and the limitation that the ingrowth is insufficient in a three-dimensional cell culturing process is solved.
Description
Technical field
The utility model is mainly used in preclinical medicine, medicament research and development and other clinical research, is specially full-automatic three-dimensional cell culture system.
Background technology
Traditional static cell cultures is carried out in culturing bottle or ware.No matter be that cell or tissue is all grown in two dimensional surface space and contacts glass or frosting.Such mode can affect the expression of gene in cell and cannot continue growth and differentiation.Meanwhile, also can there is " dedifferenting " phenomenon in the cell cultures of plane, makes cultured cells lose gradually many physiological characteristics of Qi Lai source tissue.And in most of dynamic cultivation system, cell or tissue has the external force of physics to suspend, have manyly to comprise that the factor of liquid shearing force can cause cell and tissue injury.
Utility model content
The technical problem that the utility model solves is to provide a kind of full-automatic three-dimensional cell culture system, to solve the problem proposing in above-mentioned background technology.
The technical problem that the utility model solves realizes by the following technical solutions:
Full-automatic three-dimensional cell culture system, comprises pedestal, power supply adaptor, culture dish; In described pedestal, rotation motor is housed; Described power supply adaptor is provided with tachometer gage; Described culture dish is culture plate or cultivation tubing string, and culture plate or cultivation tubing string dorsal part are equipped with silica gel gas-exchange membrane; Described culture dish is contained on pedestal; Described pedestal is connected with power supply adaptor.
Further, described pedestal comprises single rotary head pedestal, two rotary head pedestal, four rotary head pedestals, eight rotary head pedestals.
Further, described culture dish comprises STLV type culture dish, HARV type culture dish.
Further again, described HARV type culture dish comprises reusable culture dish and batch cultur ware, and reusable culture dish is used for cultivating suspension and attached cell.
Further again, described STLV type culture dish is reusable culture dish, is applicable to micro-carriers cell culture and explantation tissue and cultivates.
Further again, described batch cultur ware, every four batch cultur wares, one bag, each culture dish is packed separately, has two kinds of specifications of 10ml and 50ml.
Beneficial effect
Cell or tissue in the utility model is the state suspension with freely falling body in process of growth, there is no the destructive pressure such as agitator, bubble, therefore be organized in, in nutrient solution, be able to free-falling, upset and fully mix with nutrient solution, in its container, the strength of all directions reaches balance, so cell or tissue can not be subject to the strength impact of single direction, can evenly grow towards any direction, increase cell proliferation speed, reduce necrocytosis and effectively increase the system that cellular products is secreted, having solved the not enough restriction of interior life (ingrowth) that three-dimensional cell is cultivated.
Accompanying drawing explanation
Fig. 1 is the utility model structure iron.
Embodiment
In order to make of the present utility modelly to realize technique means, creation characteristic, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the utility model.
As shown in Figure 1, full-automatic three-dimensional cell culture system, comprises pedestal 1, power supply adaptor 2, culture dish 3; In described pedestal 1, rotation motor is housed; Described power supply adaptor 2 is provided with tachometer gage 4; Described culture dish 3 is culture plate or cultivation tubing string, and culture plate or cultivation tubing string dorsal part are equipped with silica gel gas-exchange membrane 5; Described culture dish 3 is contained on pedestal 1; Described pedestal 1 is connected with power supply adaptor 2.
Further, described pedestal 1 comprises single rotary head pedestal, two rotary head pedestal, four rotary head pedestals, eight rotary head pedestals.
Further, described culture dish 3 comprises STLV type culture dish, HARV type culture dish.
Further again, described HARV type culture dish comprises reusable culture dish and batch cultur ware, and reusable culture dish is used for cultivating suspension and attached cell.
Further again, described STLV type culture dish is reusable culture dish, is applicable to micro-carriers cell culture and explantation tissue and cultivates.
Further again, described batch cultur ware, every four batch cultur wares, one bag, each culture dish is packed separately, has two kinds of specifications of 10ml and 50ml.
The utility model principle of work: the utility model utilizes culture dish 3 to cultivate, adds nutrient solution, cell or tissue in culture dish 3 together, and removes all bubbles.Culture dish 3 is arranged on pedestal 1, and rotation motor is equipped with in 1 inside, base station, and interior tissue, cell or cell mass are because keeping suspended state under rolling tangential strength and gravity double influence.Along with cell or tissue, grow up, speed of rotation can adjust, and after cell forms agglomerate, must improve rotating speed and make it can sedimentation and bottom touching.The object of rotation is to allow all cells evenly exchange nutrient and gas, and can have enough contacts between cell and cell, is conducive to cell aggregation.In addition, culture dish dorsal part all possesses ventilation film that silica gel makes in order to carrying out gaseous interchange, makes cell or tissue obtain abundant oxygen and gets rid of the waste gas after metabolism.
More than show and described ultimate principle of the present utility model and principal character and advantage of the present utility model.The technician of the industry should understand; the utility model is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present utility model; do not departing under the prerequisite of the utility model spirit and scope; the utility model also has various changes and modifications, and these changes and improvements all fall within the scope of claimed the utility model.Claimed scope of the present utility model is defined by appending claims and equivalent thereof.
Claims (3)
1. full-automatic three-dimensional cell culture system, is characterized in that, comprises pedestal, power supply adaptor, culture dish; In described pedestal, rotation motor is housed; Described power supply adaptor is provided with tachometer gage; Described culture dish is culture plate or cultivation tubing string, and culture plate or cultivation tubing string dorsal part are equipped with silica gel gas-exchange membrane; Described culture dish is contained on pedestal; Described pedestal is connected with power supply adaptor; Described culture dish comprises STLV type culture dish, HARV type culture dish; Described HARV type culture dish comprises reusable culture dish and batch cultur ware.
2. full-automatic three-dimensional cell culture system according to claim 1, is characterized in that, described pedestal comprises single rotary head pedestal, two rotary head pedestal, four rotary head pedestals, eight rotary head pedestals.
3. full-automatic three-dimensional cell culture system according to claim 1, is characterized in that, described batch cultur ware, and every four batch cultur wares are a bag, have two kinds of specifications of 10ml and 50ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320470380.6U CN203559059U (en) | 2013-08-05 | 2013-08-05 | Full-automatic three-dimensional cell culturing system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320470380.6U CN203559059U (en) | 2013-08-05 | 2013-08-05 | Full-automatic three-dimensional cell culturing system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203559059U true CN203559059U (en) | 2014-04-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320470380.6U Expired - Fee Related CN203559059U (en) | 2013-08-05 | 2013-08-05 | Full-automatic three-dimensional cell culturing system |
Country Status (1)
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| CN (1) | CN203559059U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628370A (en) * | 2018-12-30 | 2019-04-16 | 深圳光彩生命工程技术有限公司 | A kind of Three-dimensional cell culture method |
-
2013
- 2013-08-05 CN CN201320470380.6U patent/CN203559059U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109628370A (en) * | 2018-12-30 | 2019-04-16 | 深圳光彩生命工程技术有限公司 | A kind of Three-dimensional cell culture method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140423 Termination date: 20160805 |
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| CF01 | Termination of patent right due to non-payment of annual fee |