CN215480974U - In-vitro dynamic culture device for recombining three-dimensional skin model - Google Patents
In-vitro dynamic culture device for recombining three-dimensional skin model Download PDFInfo
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- CN215480974U CN215480974U CN202121743082.0U CN202121743082U CN215480974U CN 215480974 U CN215480974 U CN 215480974U CN 202121743082 U CN202121743082 U CN 202121743082U CN 215480974 U CN215480974 U CN 215480974U
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Abstract
The utility model discloses an in vitro dynamic culture device for recombining a three-dimensional skin model, which comprises a cell culture dish and a magnetic stirrer arranged below the cell culture dish, wherein a suspended cell culture chamber and a support matched with the suspended cell culture chamber are arranged in the cell culture dish, the support comprises a panel and a plurality of supporting legs arranged below the panel, a through hole matched with the diameter of the suspended cell culture chamber is formed in the panel, so that the suspended cell culture chamber is suspended on the panel through the through hole, a magnetic stirrer matched with the magnetic stirrer is also arranged in the cell culture dish, and the magnetic stirrer is arranged below the support. The utility model realizes the maintenance of the flowing state of the culture solution, thereby simulating the natural microenvironment of tissue growth and development, promoting the growth and metabolism of cells, providing the stimulation of shearing force for engineering tissues and improving the biomechanical characteristics of the engineering tissues.
Description
Technical Field
The utility model relates to the technical field of tissue engineering, in particular to an in-vitro dynamic culture device for a recombinant three-dimensional skin model.
Background
Tissue engineering is a discipline that combines cell in vitro culture with materials to reconstitute tissues or organs in vitro. A recombined three-dimensional skin model is prepared through taking a small amount of living skin tissue from human body, enzyme digestion to separate and purify the cells from skin tissue, culturing in vitro to obtain a great amount of seed cells, adhering the skin cells to biologic material or scaffold, culturing and inducing to form artificial skin tissue with the function similar to that of natural structure of human skin.
The existing technology for constructing and culturing the recombinant three-dimensional skin in vitro mainly adopts the traditional static culture method. The growth conditions of the recombinant three-dimensional skin cultured by the static culture method are different from the natural physiological microenvironment, and the cultured skin tissues usually have differences from the original living tissues in the aspects of nutrition supply, pressure resistance, toughness and the like.
Therefore, it is necessary to provide a further solution to the above problems.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide an in-vitro dynamic culture device for recombining a three-dimensional skin model, so as to overcome the defects in the prior art.
In order to solve the technical problems, the technical scheme of the utility model is as follows:
the utility model provides an in vitro dynamic culture apparatus for recombining three-dimensional skin model, include the cell culture dish with set up in the magnetic stirrers of cell culture dish below, be provided with suspension type cell culture cell in the cell culture dish and with the support of suspension type cell culture cell looks adaptation, the support include the panel with set up in a plurality of supporting legss of panel below, set up on the panel with the through-hole of the diameter looks adaptation of suspension type cell culture cell, so that suspension type cell culture cell passes through the through-hole hang in on the panel, still placed in the cell culture dish with the magnetic stirrers of magnetic stirrers looks adaptation, the magnetic stirrers is arranged in the below of support.
In a preferred embodiment of the present invention, the rotation speed of the magnetic stirrer is 5 to 200 rpm.
In a preferred embodiment of the present invention, the material of the bracket is 304 steel.
In a preferred embodiment of the utility model, the suspended cell culture chamber is a 6-well plate, a 12-well plate or a 24-well plate.
In a preferred embodiment of the present invention, the apparatus further comprises a carbon dioxide incubator for accommodating the cell culture dish and the magnetic stirrer.
In a preferred embodiment of the present invention, the supporting leg includes a tube casing connected to the panel and a leg slidably disposed in the tube casing, a guide groove is formed in a side surface of the tube casing in an extending manner along a height direction of the tube casing, a plurality of limiting grooves are sequentially formed in the side surface of the tube casing along the height direction of the tube casing, the limiting grooves are communicated with the guide groove, a limiting rod is disposed on a side edge of the leg, and a size of the limiting rod is matched with a size of the guide groove and a size of the limiting groove, so that the limiting rod slides in the guide groove and the limiting groove.
In a preferred embodiment of the utility model, the section of the limiting rod is square.
In a preferred embodiment of the utility model, the bottom end of the leg post is provided with a horizontal extension block, and the edge of the horizontal extension block protrudes out of the edge of the pipe sleeve.
Compared with the prior art, the utility model has the beneficial effects that:
(1) the dynamic culture of the recombinant three-dimensional skin model is realized by matching the cell culture dish and the magnetic stirrer, and the magnetic stirrer is specifically used for controlling the magnetic stirrer in the cell culture dish to stir the culture solution in the cell culture dish and keeping the flowing state of the culture solution, so that the natural microenvironment for the growth and development of tissues is simulated, the growth and metabolism of cells are promoted, the stimulation of shearing force is provided for the engineering tissues, and the biomechanical characteristics of the engineering tissues can be improved; furthermore, the stirring speed of the device is controllable, so that stress stimulation with different strengths is realized, and the culture effect is improved.
(2) According to the utility model, the support is arranged in the cell culture dish, so that the suspension support of the suspension type cell culture chamber is realized, the support can be repeatedly used, and the culture cost is reduced; furthermore, the height of the support is adjusted through the supporting legs, so that the support is suitable for suspension type cell culture cells of different models and cell culture dishes of different models, and the utilization rate is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic, disassembled view of the present invention, with the suspended cell culture chamber omitted;
FIG. 2 is a schematic perspective view of the present invention taken at another angle;
fig. 3(a) - (b) are schematic perspective views of the supporting leg of the present invention in different states.
Specifically, 100, a cell culture dish;
200. a support; 210. a panel; 211. a through hole; 220. supporting legs; 221. pipe sleeve; 2211. a guide groove; 2212. a limiting groove; 222. a socle; 2221. a limiting rod; 2222. a horizontally extending block;
300. a magnetic stirrer; 310. and (4) magnetically stirring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be taken as limiting the scope of the present invention. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the utility model, the meaning of "a plurality" is two or more unless otherwise specified.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
As shown in fig. 1 and fig. 2, an in vitro dynamic culture apparatus for reconstructing a three-dimensional skin model comprises a cell culture dish 100 (including a cover, not shown in the figures) and a magnetic stirrer 300 arranged below the cell culture dish 100, wherein a magnetic stirrer 310 adapted to the magnetic stirrer 300 is further placed in the cell culture dish 100. Preferably, the cell culture dish 100 has a diameter of not less than 150mm to accommodate large volume cell culture.
This device utilizes magnetic stirrers 300 to control the stirring of the culture solution in cell culture dish 100 of magnetic stirrers 310 in cell culture dish 100, keeps the mobile state of culture solution to the natural microenvironment of simulation tissue growth development promotes the growth metabolism of cell, and provides the stimulation of shearing force for engineering tissue, can improve the biomechanics characteristic of engineering tissue. And the stirring speed of the device is controllable, so that stress stimulation with different strengths is realized, and the culture effect is improved. Preferably, the device adopts a low-speed magnetic stirrer 300, and the specific rotating speed is 5-200 rpm so as to adapt to three-dimensional skin models with different culture requirements.
The apparatus may further include a carbon dioxide incubator for accommodating the cell culture dish 100 and the magnetic stirrer 300, which is a prior art and will not be described herein. And a carbon dioxide incubator with constant temperature control of 37 ℃ is preferably adopted to realize better simulation of human body environment and facilitate cell culture.
The cell culture dish 100 of the device is provided with a suspended cell culture chamber (not shown in the figure) and a bracket 200 matched with the suspended cell culture chamber, and the bracket 200 is used for suspending and supporting the suspended cell culture chamber. Suspension cell culture chambers are cell culture devices commonly used in the art and will not be described in detail herein. Preferably, the model of the suspended cell culture chamber in the device is 6-well plate, 12-well plate or 24-well plate to meet most experimental requirements, and the support 200 in the device is matched with the model of the suspended cell culture chamber.
As shown in fig. 2 and 3, the stand 200 of the present device includes a panel 210 and a plurality of support legs 220 disposed below the panel 210. The faceplate 210 is provided with a through hole 211 adapted to the diameter of the suspended cell culture chamber, so that the suspended cell culture chamber is suspended on the faceplate 210 through the through hole 211. In the present embodiment, the panel 210 is circular, but is not limited thereto, and the panel 210 may also be square, rectangular or any other geometric figure. A magnetic stirrer 310 is generally placed under the support 200 to sufficiently stir the culture solution. The material of the bracket 200 is preferably 304 steel to avoid affecting cell culture and facilitate reuse.
The bracket 200 and the magnetic stirrer 310 in the device can be cleaned, sterilized and reused, thereby greatly reducing the culture cost.
As shown in fig. 3(a) - (b), the supporting leg 220 preferably comprises a tube sleeve 221 connected to the panel 210 and a leg 222 slidably disposed in the tube sleeve 221, i.e. the tube sleeve 221 can drive the panel 210 to move upward relative to the leg 222, thereby increasing the height of the panel 210 to accommodate different types of suspension cell culture chambers and different types of cell culture dishes 100, improving the utilization rate, specifically accommodating suspension cell culture chambers with different depths and cell culture dishes 100 with different depths, and adjusting the distance between the magnetic stirrer 310 and the bottom surface of the suspension cell culture chamber.
In order to stabilize the relative positions of the pipe sleeve 221 and the leg 222, the two are connected by the guide slot 2211, the limit slot 2212 and the limit rod 2221. Specifically, the side of the pipe sleeve 221 is provided with a guide groove 2211 extending along the height direction thereof, the side of the pipe sleeve 221 is provided with a plurality of limiting grooves 2212 sequentially along the height direction thereof, the limiting grooves 2212 are communicated with the guide groove 2211, the side of the leg 222 is provided with a limiting rod 2221, and the size of the limiting rod 2221 is matched with the size of the guide groove 2211 and the size of the limiting groove 2212, so that the limiting rod 2221 slides in the guide groove 2211 and the limiting groove 2212. Further, the cross section of the stop bar 2221 is square, so that the contact area between the stop bar 2221 and the guide groove 2211 or the stop groove 2212 is increased, friction is increased, and the stop bar 2221 is prevented from sliding by mistake. Still further, the bottom end of the leg 222 is provided with a horizontal extension block 2222, and the edge of the horizontal extension block 2222 protrudes beyond the edge of the tube sleeve 221, so as to increase the contact area between the leg 222 and the bottom surface of the cell culture dish 100, so that the bracket 200 is more stable, and the tube sleeve 221 is prevented from directly impacting the bottom surface of the cell culture dish 100, which may damage the cell culture dish 100.
In summary, the dynamic culture of the recombinant three-dimensional skin model is realized through the cooperation of the cell culture dish and the magnetic stirrer, and the magnetic stirrer is specifically used for controlling the magnetic stirrer in the cell culture dish to stir the culture solution in the cell culture dish and keeping the flowing state of the culture solution, so that the natural microenvironment for the growth and development of the tissue is simulated, the growth and metabolism of cells are promoted, the stimulation of shearing force is provided for the engineering tissue, and the biomechanical characteristics of the engineering tissue can be improved.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. The utility model provides an in vitro dynamic culture device for recombining three-dimensional skin model, a serial communication port, including the cell culture dish with set up in the magnetic stirrers of cell culture dish below, be provided with suspension type cell culture cell in the cell culture dish and with the support of suspension type cell culture cell looks adaptation, the support include the panel with set up in a plurality of supporting legss of panel below, set up on the panel with the through-hole of the diameter looks adaptation of suspension type cell culture cell, so that suspension type cell culture cell passes through the through-hole hang in on the panel, still placed in the cell culture dish with the magnetic stirrers of magnetic stirrers looks adaptation, the magnetic stirrers is arranged in the below of support.
2. The in vitro dynamic culture device for the recombinant three-dimensional skin model according to claim 1, wherein the rotation speed of the magnetic stirrer is 5-200 rpm.
3. The in vitro dynamic culture apparatus for the recombinant three-dimensional skin model according to claim 1, wherein the scaffold is made of 304 steel.
4. The in vitro dynamic culture apparatus for the recombinant three-dimensional skin model according to claim 1, wherein the suspended cell culture chamber is a 6-well plate, a 12-well plate or a 24-well plate.
5. The in vitro dynamic culture apparatus for restructuring the three-dimensional skin model according to claim 1, further comprising a carbon dioxide incubator for accommodating the cell culture dish and the magnetic stirrer.
6. The in vitro dynamic culture device for the recombination of the three-dimensional skin model according to claim 1, wherein the support leg comprises a tube sleeve connected with the panel and a leg post slidably disposed in the tube sleeve, a guide groove is formed in the side surface of the tube sleeve along the height direction of the tube sleeve, a plurality of limiting grooves are sequentially formed in the side surface of the tube sleeve along the height direction of the tube sleeve, the limiting grooves are communicated with the guide groove, a limiting rod is disposed on the side edge of the leg post, and the size of the limiting rod is matched with the size of the guide groove and the size of the limiting groove, so that the limiting rod slides in the guide groove and the limiting groove.
7. The in vitro dynamic culture device for the recombinant three-dimensional skin model according to claim 6, wherein the section of the limiting rod is square.
8. The in vitro dynamic culture apparatus for the skin restructuring three-dimensional model according to claim 6, wherein the bottom end of the leg is provided with a horizontally extending block, and the edge of the horizontally extending block protrudes out of the edge of the tube sleeve.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121743082.0U CN215480974U (en) | 2021-07-29 | 2021-07-29 | In-vitro dynamic culture device for recombining three-dimensional skin model |
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| CN202121743082.0U CN215480974U (en) | 2021-07-29 | 2021-07-29 | In-vitro dynamic culture device for recombining three-dimensional skin model |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118001987A (en) * | 2024-04-10 | 2024-05-10 | 江苏立维斯德生物技术有限公司 | Magnetic stirrer for vaccine culture solution |
| CN118001987B (en) * | 2024-04-10 | 2024-05-31 | 江苏立维斯德生物技术有限公司 | Magnetic stirrer for vaccine culture solution |
-
2021
- 2021-07-29 CN CN202121743082.0U patent/CN215480974U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118001987A (en) * | 2024-04-10 | 2024-05-10 | 江苏立维斯德生物技术有限公司 | Magnetic stirrer for vaccine culture solution |
| CN118001987B (en) * | 2024-04-10 | 2024-05-31 | 江苏立维斯德生物技术有限公司 | Magnetic stirrer for vaccine culture solution |
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