CN213388708U

CN213388708U - 3D cell culture device based on SIS

Info

Publication number: CN213388708U
Application number: CN202022111687.XU
Authority: CN
Inventors: 万建; 尹路; 陈春球; 袁骁祺; 张子超
Original assignee: Shanghai Tenth Peoples Hospital
Current assignee: Shanghai Tenth Peoples Hospital
Priority date: 2020-09-23
Filing date: 2020-09-23
Publication date: 2021-06-08
Anticipated expiration: 2030-09-23

Abstract

The utility model discloses a 3D cell culture device based on SIS, including supporting the base, training cell and SIS pig small intestine mucous membrane lower floor, annular slot has been seted up at the top of supporting the base, it pegs graft inside annular slot to train the cell, the top of training the cell runs through annular slot and extends to its outside, is located the inside cultivation cell both sides of annular slot and all sets up threaded hole, SIS pig small intestine mucous membrane lower floor lays in the inside that supports the base top and be located the cultivation cell, the connecting hole has all been seted up to the position that supports the base both sides and correspond the threaded hole, the inside of connecting hole is equipped with connecting screw. This 3D cell culture device based on SIS, structural design is reasonable, and maneuverability is strong, low in manufacturing cost, and it is good to cultivate stability, can satisfy diversified demand, provides simple and easy mode for the 3D of cell cultivates.

Description

3D cell culture device based on SIS

Technical Field

The utility model relates to a cell culture equipment technical field specifically is a 3D cell culture device based on SIS.

Background

Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an indispensable process for both the whole biotechnology and one of the biological cloning technologies, and is a large-scale clone of cells, and the cell culture technology can be a simple single cell or a few differentiated multiple cells through mass culture of one cell, which is an indispensable link of the cloning technology, and is a clone of cells, and a large amount of cells or metabolites thereof are obtained through cell culture, and the biological products are all obtained from the cells, so the cell culture technology can be said to be the most core and basic technology in the biotechnology.

SIS is prepared by eluting small intestines of pigs with chemical eluent, SIS is a natural extracellular matrix material which is rich in various growth factors, has good mechanical properties, histocompatibility and lower immunogenicity, and can be used for 3D culture of cells to simulate a real in-vivo environment.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a 3D cell culture device based on SIS to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a 3D cell culture device based on SIS, is including supporting the base, cultivateing cell and SIS pig small intestine mucous membrane lower floor, the annular slot has been seted up at the top of supporting the base, cultivate the cell and peg graft inside annular slot, the top of cultivateing the cell runs through annular slot and extends to its outside, is located the inside cultivation cell both sides of annular slot and all sets up threaded hole, SIS pig small intestine mucous membrane lower floor lays at supporting the base top and is located the inside of cultivateing the cell, the position that supports the base both sides and correspond the threaded hole all sets up the connecting hole, the inside of connecting hole is equipped with connecting screw, connecting screw is close to the one end of cultivateing the cell and runs through the connecting hole in proper order, supports base and threaded hole and extends to threaded hole inside rather than threaded connection, the symmetry is equipped.

Preferably, the middle point of the bottom of the supporting base is fixedly connected with a first sucker, and the edges of the bottom of the supporting base are provided with four second suckers in an equidistance surrounding manner.

Preferably, the bottom of the left side and the bottom of the right side of the supporting base are both fixedly connected with a first handle, and one end, far away from the supporting base, of the connecting screw is fixedly connected with a second handle.

Preferably, the equal fixedly connected with location slider in the bottom of cultivateing the cell left and right sides, support the base and seted up the location spout rather than the cooperation is used corresponding to the position of location slider.

Preferably, the support base and the culture chamber are both made of PVC materials, and the culture chamber is in a cylindrical shape with a hollow middle.

Preferably, the thickness of the polycarbonate film is specifically 0.22 um.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. the utility model discloses a cut SIS pig small intestine submucosal layer to and support the area of base equidimension, the tiling is on supporting the base, to cultivate the cell and peg graft in annular slot, and insert the support base in the lump with the SIS edge, utilize connecting screw to connect, it is fixed to realize, can add the cell suspension when cultivateing in the cell, the cell encloses the cell on SIS, can effectively promote the cell field planting in SIS, utilize the 0.22um polycarbonate membrane of cultivateing cell side setting, when isolated cell is external to be scattered, do not influence the culture medium with in the culture dish and carry out the nutrient substance exchange, this 3D cell culture apparatus based on SIS, structural design is reasonable, the maneuverability is strong, low in manufacturing cost, and culture stability is good, can satisfy diversified demand, 3D for the cell cultivates and provides simple and easy mode.

2. The utility model discloses a set up first sucking disc and second sucking disc, can effectively adsorb culture apparatus and fix on the mesa, bump and lead to it to rock or drop to prevent the mistake, improved culture apparatus's stability, through setting up the first in command and the second in command, the first in command is convenient for take the device, the second in command is convenient for rotate connecting screw, through setting up location slider and location spout, can be so that the screw hole of cultivateing the cell both sides just to the connecting hole, and then realize connecting screw's installation.

Drawings

FIG. 1 is a sectional view of the front view of the present invention;

FIG. 2 is an enlarged view of a portion of A-A of FIG. 1 according to the present invention;

fig. 3 is a schematic structural diagram of a top view of the support base of the present invention;

fig. 4 is a schematic structural view of a bottom view of the support base of the present invention;

FIG. 5 is a schematic structural view of a top view of the culture chamber of the present invention.

In the figure: the device comprises a support base 1, a culture chamber 2, a 3SIS pig small intestine submucosa, a 4 annular slot, a 5 threaded hole, a 6 connecting hole, a 7 connecting screw, an 8 polycarbonate film, a 9 first sucker, a 10 second sucker, a 11 first handle, a 12 second handle, a 13 positioning sliding block and a 14 positioning sliding groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-5, a SIS-based 3D cell culture device comprises a support base 1, a culture chamber 2 and a SIS porcine small intestine submucosa 3, wherein a first suction cup 9 is fixedly connected to the middle point of the bottom of the support base 1, four second suction cups 10 are arranged at the edge of the bottom of the support base 1 in a surrounding manner at equal intervals, the culture device can be effectively adsorbed and fixed on a table top by arranging the first suction cups 9 and the second suction cups 10 to prevent the culture device from shaking or falling due to accidental collision, the stability of the culture device is improved, a first handle 11 is fixedly connected to the bottoms of the left side and the right side of the support base 1, the first handle 11 is convenient for holding the device, an annular slot 4 is arranged at the top of the support base 1, the support base 1 and the culture chamber 2 are both made of PVC material, the culture chamber 2 is in a cylindrical shape with a hollow middle part, the culture chamber 2 is inserted into the annular slot, the top of the culture chamber 2 penetrates through the annular slot 4 and extends to the outside of the annular slot, the bottoms of the left side and the right side of the culture chamber 2 are fixedly connected with positioning sliders 13, the positions, corresponding to the positioning sliders 13, of the supporting base 1 are provided with positioning chutes 14 matched with the positioning sliders, the two sides, located inside the annular slot 4, of the culture chamber 2 are provided with threaded holes 5, the SIS pig small intestine submucosal layer 3 is laid on the top of the supporting base 1 and located inside the culture chamber 2, the positions, corresponding to the threaded holes 5, of the two sides of the supporting base 1 are provided with connecting holes 6, connecting screws 7 are arranged inside the connecting holes 6, one end, far away from the supporting base 1, of each connecting screw 7 is fixedly connected with a second handle 12, the second handle 12 is convenient for rotating the connecting screws 7, one end, close to the culture chamber 2, of each connecting screw 7 sequentially penetrates through, through setting up location slider 13 and location spout 14, can be so that cultivate the screw hole 5 of 2 both sides of cell just to connecting hole 6, and then realize connecting screw 7's installation, the symmetry is equipped with polycarbonate membrane 8 on the inner wall of cultivation cell 2, and polycarbonate membrane 8's thickness specifically is 0.22um, and the medium that does not influence in with the culture dish when preventing that the cell is external scatters carries out the nutritive substance exchange.

During the use, cut SIS pig small intestine submucosa 3 to the area with support base 1 equidimension, the tiling is on supporting base 1, peg graft culture cell 2 in annular slot 4, and insert support base 1 with SIS edge together, utilize connecting screw 7 to connect, realize fixedly, can add cell suspension in culture cell 2 when cultivateing, the cell encloses the cell on SIS, can effectively promote cell field planting in SIS, utilize the 0.22um polycarbonate membrane 8 that culture cell 2 side set up, when isolated cell is scattered, do not influence the culture medium with in the culture dish and carry out the nutrient substance exchange, culture cell 2 can be according to the cultivation demand simultaneously, prepare into different specifications, 6 orifice plates are put into from the convenience, 12, cell 3D cultivates in the 24 orifice plates.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a 3D cell culture device based on SIS, includes support base (1), culture chamber (2) and SIS pig small intestine submucosa (3), its characterized in that: the top of the supporting base (1) is provided with an annular slot (4), the culture chamber (2) is inserted into the annular slot (4), the top of the culture chamber (2) penetrates through the annular slot (4) and extends to the outside of the annular slot, both sides of the culture chamber (2) positioned in the annular slot (4) are provided with threaded holes (5), the SIS pig small intestine submucosal layer (3) is laid on the top of the supporting base (1) and positioned in the culture chamber (2), both sides of the supporting base (1) and corresponding to the positions of the threaded holes (5) are provided with connecting holes (6), the connecting holes (6) are internally provided with connecting screws (7), one ends of the connecting screws (7) close to the culture chamber (2) sequentially penetrate through the connecting holes (6), the supporting base (1) and the threaded holes (5) and extend to the inside of the threaded holes (5) to be in threaded connection with the threaded holes (5), and polycarbonate membranes (8) are symmetrically arranged on the inner wall of the culture chamber (2).

2. A SIS-based 3D cell culture device according to claim 1, wherein: the middle point of the bottom of the supporting base (1) is fixedly connected with a first sucker (9), and four second suckers (10) are arranged at the edge of the bottom of the supporting base (1) in an encircling mode at equal intervals.

3. A SIS-based 3D cell culture device according to claim 2, wherein: the bottom of the left side and the bottom of the right side of the supporting base (1) are both fixedly connected with a first handle (11), and one end of the connecting screw (7) far away from the supporting base (1) is fixedly connected with a second handle (12).

4. A SIS-based 3D cell culture device according to claim 3, wherein: the equal fixedly connected with location slider (13) in the bottom of cultivateing cell (2) left and right sides, support base (1) and seted up location spout (14) rather than the cooperation use corresponding to the position of location slider (13).

5. A SIS-based 3D cell culture device according to claim 4, wherein: the support base (1) and the culture chamber (2) are both made of PVC materials, and the culture chamber (2) is in a cylindrical shape with a hollow middle.

6. A SIS-based 3D cell culture device according to claim 5, wherein: the thickness of the polycarbonate film (8) is specifically 0.22 um.