CN209098696U - A kind of cell scoring devices - Google Patents
A kind of cell scoring devices Download PDFInfo
- Publication number
- CN209098696U CN209098696U CN201821784575.7U CN201821784575U CN209098696U CN 209098696 U CN209098696 U CN 209098696U CN 201821784575 U CN201821784575 U CN 201821784575U CN 209098696 U CN209098696 U CN 209098696U
- Authority
- CN
- China
- Prior art keywords
- barrier piece
- piece
- barrier
- cell
- scoring devices
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of cell scoring devices, the cell scoring devices are made of several piece barrier piece arranged in parallel, the distance between adjacent barrier piece is equal, by being fixedly connected at the top of barrier piece with the vertically disposed several connection straps of barrier piece between barrier piece, position corresponding with connection strap is equipped with groove at the top of barrier piece, the connection strap is placed in groove, is equipped with several through-holes in the lower section of each barrier piece.Cell scoring devices disclosed by the utility model design it is simple, low in cost, easy to use, without additional device auxiliary or increase experiment flow and reusable, when carrying out scratch experiment, the svelteness of acquisition is beautiful, experimental error is small, meet requirement of experiment, provides experiment basis for more accurate detection tumor cell invasion ability.
Description
Technical field
The utility model belongs to field of cell culture, more particularly to a kind of cell scoring devices.
Background technique
Cell culture refers to simulated in vivo environment (sterile, preference temperature, pH value and certain nutritional condition etc.) in vitro,
It is allowed to survive, grow, breed and maintain a kind of method of primary structure and function.Cell culture technology day crescent at this stage
It is different, wherein cell scratch experiment is a kind of method of simple and easy detection cell movement, and experimental cost is low, can be used to examine
Survey the invasion transfer ability of adherent growth tumour cell.The method that cell scratch experiment uses manual drawing straight line more at present, i.e.,
By cell seeding in culture dish, anti-tumor drug is added after cell covers with, then draws in culture dish and takes several straight lines, is gone
Except the cell fragment in scratch, the tumour cell after observing drug effect puts the invasion feelings in scored area in different times
Condition, and invasion area of the tumour cell in scratch is calculated, to evaluate the tumor cell invasion energy after drug effect
Power.
But the conventional method is increasingly difficult to meet the requirement of cell scratch experiment, disadvantage shows following side
Face: (1) lines are bent, and the adherent tighter tumour cell of residual is easy in scratch；(2) manual scratch is easy to lead there are blindness
Cause accuracy that is irregular and influencing experimental data of crossing；(3) manual operations is cumbersome, there is a possibility that increasing cell contamination；(4)
If experiment accuracy is under suspicion, a large amount of scientific research human and material resources and financial resources are wasted.In recent years, the scientific research of many heavyweights
Magazine is because the method for manual drawing straight line is easy to influence the accuracy of experimental data, and refuses to receive the section in relation to such experiment
Grind paper.The scientific research personnel for being engaged in tumor research has a profound understanding of cell scratch experiment significance in tumor invasion.
Therefore, it needs to design one kind scoring devices that are novel, practical and meeting requirement of experiment to solve existing skill
The problem of art, this also will be helpful to the development of tumor invasion Journal of Sex Research.
Utility model content
In view of the above problems, the utility model is intended to provide a kind of cell scoring devices, and structure is simple, user
Just, the svelteness of acquisition, beauty, neat, provides experiment basis for more accurate detection tumor cell invasion ability.
To achieve the goals above, technical solution used by the utility model is as follows: a kind of cell scoring devices, described
Cell scoring devices are made of several piece barrier piece arranged in parallel, and the height of barrier piece is lower than the height of culture dish, adjacent barrier
The distance between piece is equal, by being fixedly connected at the top of barrier piece with the vertically disposed several connection straps of barrier piece between barrier piece,
Position corresponding with connection strap is equipped with groove at the top of barrier piece, and the connection strap is placed in groove, under each barrier piece
Side is equipped with several through-holes, the specific location of through-hole can also be at middle part in the end of barrier piece, the shape of through-hole, which is not also done, to be had
Body limit, it is round, rectangular, as long as using when can make culture solution by through-hole it is i.e. applicable.
Further, the production material of the barrier piece and connection strap includes glass, stainless steel or plastics, herein to accessory
Material is not particularly limited, as long as can meet the isolation fixed function of barrier piece and connection strap respectively.
Further, the barrier piece includes glass glue connection with the mode that is fixedly connected between connection strap, and glass cement is fixed
Only one of connection type, other connection types can also be with, as long as being able to achieve being fixedly connected for barrier piece and connection strap
?.
Further, when the barrier piece quantity is odd number, the length of intermediate barrier piece and the inner ring diameter of culture dish
It is adapted, the symmetrical setting of barrier piece of two sides, and the length of barrier piece is sequentially reduced from centre to two sides, every piece of side barrier
The length profile of culture dish inner ring is adapted in the length of piece and the barrier leaf length extending direction.
Further, when the barrier piece quantity is even number, the symmetrical setting of barrier piece, the length of barrier piece from centre to
Two sides are sequentially reduced, and the length of every piece of barrier piece and the length profile of culture dish inner ring on the barrier leaf length extending direction are mutually fitted
It answers.
A kind of application method of cell scoring devices, specifically used step are as follows:
1) before cell inoculation, first cell scoring devices are subjected to autoclave sterilization in autoclave, then stored
It is spare in super-clean bench；
2) cell scoring devices are placed in culture dish, confirmation device is put neatly, and pairing is tight, then will be processed
The cell inoculation of logarithmic growth phase is trained in incubator after being mixed the cell piping and druming in culture dish with suction pipe in culture dish
It supports；
3) when it is 50 ~ 60% that cell, which grows to density, drug-treated is carried out, selectivity replaces culture medium, continues to be placed in training
It supports and is cultivated in case；
4) when cell density is 80 ~ 90%, cell scoring devices is removed, anti-tumor drug is then added, is removing
Different time sections after device in microscopically observation tumour cell through related drugs treated invasive ability, and note of taking pictures
Record, is compared different time points cell invasion area further according to requirement of experiment, obtains experiment conclusion.
The beneficial effects of the utility model are:
1. carry out scratch experiment using cell scoring devices disclosed by the utility model, the svelteness of acquisition, beauty,
Neat and diameter is equal, under the microscope observing effect are as follows: the diameter between straight, rule, different lines is identical, lines two sides
Cell is uniform；Because being equidistant between each straight line, drug concentration used, cell density are identical, and experimental error is small, meets experiment
It is required that；Cell gradually covers with scored area after removing device, provides reality for more accurate detection tumor cell invasion ability
Test basis；
2. the design of cell scoring devices is simple, the barrier of several pieces of different lengths is selected according to the difference of culture dish internal diameter
Piece composition, it is cheap；It is simple, convenient when use, without additional device auxiliary or increase experiment flow；
3. cell scoring devices Preservation in sterile condition after conventional autoclave sterilization strictly observes cell culture stream in super-clean bench
The probability of journey, cell contamination is small, and reusable；
4. cell scoring devices are easy to use: before inoculating cell enters culture dish, the cell scratch after autoclave sterilization being filled
It puts in culture dish, inoculates cell and enter culture dish；When cell grows to the density for meeting requirement of experiment, device is removed,
It can carry out microscopically observation.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of barrier piece disclosed in the utility model embodiment 1；
Fig. 2 is the structural schematic diagram of cell scoring devices disclosed in the utility model embodiment 1；
Fig. 3 is the schematic diagram of experimental group scratch after removing cell scoring devices of the utility model embodiment 3；
Fig. 4 be the utility model embodiment 3 experimental group after removing cell scoring devices 0 hour cell growth figure；
Fig. 5 be the utility model embodiment 3 experimental group after removing cell scoring devices 24 hours cell growth figures；
Fig. 6 be the utility model embodiment 3 experimental group after removing cell scoring devices 48 hours cell growth figures；
Fig. 7 be the utility model embodiment 3 control group scratch after 48 hours cell growth figures.
Wherein, 1- barrier piece, 2- connection strap, 3- through-hole, 4- cell, 5- scratch straight line.
Specific embodiment
In order to make those skilled in the art be better understood on the technical solution of the utility model, below with reference to attached
Figure and embodiment are further described the technical solution of the utility model.
Embodiment 1
Cell scoring devices disclosed in the present embodiment be used for 6 hole culture dishes, specific structure is as shown in Figs. 1-2, including 5 pieces every
Baffle, the height of barrier piece are lower than the height of culture dish, are parallel to each other between barrier piece, and the spacing between adjacent barrier piece is equal,
It is located in the middle barrier piece longest, other 4 pieces of barrier pieces are symmetrical arranged at left and right sides of it, length is successively passed from centre to two sides
Subtract, be fixedly connected between barrier piece by several connection straps of setting perpendicular at the top of barrier piece, at the top of barrier piece with connection
The corresponding position of item is equipped with groove, and the connection strap is placed in groove, and the lower position of every piece of barrier piece front and back end is equipped with through-hole；
Design parameter are as follows: intermediate 34.5 mm of barrier length of a film, the barrier length of a film 27.6mm in its two sides adjacent position, outermost barrier piece
Long 12.2mm.
Embodiment 2
Cell scoring devices disclosed in the present embodiment include 6 pieces of barrier pieces and 3 connection straps, in the bottom of every piece of barrier piece
Centre position is all provided with through-hole, circulates for culture solution, and barrier piece is parallel to each other, and the height of barrier piece is lower than the height of culture dish,
The spacing of adjacent barrier piece is equal, and barrier piece is symmetrically arranged in two sides, length is successively successively decreased arrangement from centre to two sides, barrier piece
Between be fixedly connected by three connection straps of setting perpendicular at the top of barrier piece, the position corresponding with connection strap at the top of barrier piece
Install fluted, the connection strap is placed in groove, and barrier piece and connection strap are fixed by glass cement.
Embodiment 3
For cultivating lung cell A549 and be inoculated in 6 orifice plates progress cell scratch experiment:
Experimental group:
1, before A549 cell inoculation, first cell scoring devices disclosed in embodiment 1 are carried out in autoclave
Then autoclave sterilization is deposited in spare in super-clean bench；
2, the A549 cell in logarithmic growth phase is discarded into culture medium, with 1 × PBS(phosphate buffered saline solution) rinse 2
Secondary, 0.25% pancreatin digests 3 ~ 5 minutes, and cell is digested from culture dish bottom, and suction pipe is sucked in 15 mL centrifuge tubes, with
Room temperature, 500 rpm centrifugation, 5 minutes collection cells carry out cell count；
3, cell scoring devices are placed in 6 orifice plates, confirmation device is put neatly, and pairing is tight, then by 1 ~ 2 × 105
A A549 cell inoculation (adjusts) in 6 orifice plates according to different requirement of experiment, blows and beats the cell in culture dish with suction pipe and mixes
It is cultivated in the incubator of 37 DEG C of+5%CO2 after even；
4, when it is 50 ~ 60% that cell, which grows to density, drug-treated is carried out, replace culture medium or culture medium need not be replaced
(requiring to adjust according to different experiments), continues to be placed in incubator and cultivate；
5, when cell density is 80 ~ 90%, cell scoring devices are removed, it is seen that culture dish bottom is clear, neat, beautiful
It sees, the scratch lines that diameter is equal, as shown in figure 3,4 be the cell in culture dish, 5 be culture dish after cell scoring devices remove
The scratch straight line of middle presentation；Then anti-tumor drug is added, 0 hour after removing device, 6 hours, 12 hours, 24 hours, 36
Hour, in microscopically observation tumour cell, through related drugs, treated invades within 48 hours (being adjusted according to different requirement of experiment)
Ability is attacked, and is photographed to record, different time points cell invasion area is compared further according to requirement of experiment, obtains experiment knot
By.
Control group do not use cell scoring devices, using traditional manual drawing straight-line method scratch, other steps with it is above-mentioned
Method is identical.
Experimental group removes 0 hour after device cell growth status such as Fig. 4, it is seen that and scratch is neat, clear, width is uniform,
Scratch two sides have no obvious cellular damage, and obvious cell intrusion is had no in scratch；Experimental group removes 24 hours after device cells
Growing state such as Fig. 5, it is seen that tumour cell starts to invade or shift into scratch after dosing；Experimental group removes after device 48 hours
Cell growth status such as Fig. 6, it is seen that scratch inner tumour cell has covered with；48 hours cells grow feelings after control group scratch
Condition such as Fig. 7, it is seen that scratch inner cell has no obvious growth.
Therefore, it using the cell scoring devices of the utility model, when the cell in culture dish grows to corresponding density, moves
Except device, visible clear, straight lines in microscopically observation plate, and lines hecatomeral cells is uniform, cell is gradually grown later
Full scored area, provides experiment basis for more accurate detection tumor cell invasion ability.
The basic principles and main features and advantage of the utility model have been shown and described above.But the foregoing is merely
Specific embodiment of the utility model, the technical characteristics of the utility model are not limited thereto, any those skilled in the art
The other embodiments obtained in the case where not departing from the technical solution of the utility model should all cover the patent model in the utility model
Among enclosing.
Claims (5)
1. a kind of cell scoring devices, which is characterized in that the cell scoring devices are made of several piece barrier piece arranged in parallel,
The height of barrier piece is lower than the height of culture dish, and the distance between adjacent barrier piece is equal, by the top of barrier piece between barrier piece
It being fixedly connected with the vertically disposed several connection straps of barrier piece, position corresponding with connection strap is equipped with groove at the top of barrier piece,
The connection strap is placed in groove, is equipped with several through-holes in the lower section of each barrier piece.
2. a kind of cell scoring devices as described in claim 1, which is characterized in that the production material of the barrier piece and connection strap
Matter includes glass, stainless steel and plastics.
3. a kind of cell scoring devices as claimed in claim 2, which is characterized in that the fixation between the barrier piece and connection strap
Connection type includes glass glue connection.
4. a kind of cell scoring devices as claimed in claim 3, which is characterized in that the barrier piece quantity is odd number, intermediate
The length of barrier piece be adapted with the inner ring diameter of culture dish, the symmetrical setting of barrier piece of two sides, and the length of barrier piece
It is sequentially reduced from centre to two sides, culture dish inner ring in the length of the every piece of barrier piece in side and the barrier leaf length extending direction
Length profile is adapted.
5. a kind of cell scoring devices as claimed in claim 3, which is characterized in that the barrier piece quantity is even number, barrier
The symmetrical setting of piece, the length of barrier piece are sequentially reduced from centre to two sides, the length of every piece of barrier piece and the barrier leaf length
The length profile of culture dish inner ring is adapted on extending direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821784575.7U CN209098696U (en) | 2018-10-31 | 2018-10-31 | A kind of cell scoring devices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821784575.7U CN209098696U (en) | 2018-10-31 | 2018-10-31 | A kind of cell scoring devices |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209098696U true CN209098696U (en) | 2019-07-12 |
Family
ID=67157542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821784575.7U Active CN209098696U (en) | 2018-10-31 | 2018-10-31 | A kind of cell scoring devices |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209098696U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234143A (en) * | 2018-10-31 | 2019-01-18 | 扬州大学 | A kind of cell scoring devices and its application method |
-
2018
- 2018-10-31 CN CN201821784575.7U patent/CN209098696U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109234143A (en) * | 2018-10-31 | 2019-01-18 | 扬州大学 | A kind of cell scoring devices and its application method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN209098696U (en) | A kind of cell scoring devices | |
| CN103614411A (en) | Research method for American ginseng hairy root induction and plant regeneration | |
| CN105861441B (en) | A kind of liver cancer cell lines STL-C1 from human hepatocellular carcinoma cancer beside organism and its build system, method | |
| CN103849602A (en) | Bovine testicle cell line as well as establishment method and application thereof | |
| CN102952751A (en) | Co-culture support of multiple types of cells | |
| CN201952427U (en) | Artificial oral-cavity simulating system device | |
| CN109234143A (en) | A kind of cell scoring devices and its application method | |
| CN106754690A (en) | A kind of chromosome culture medium of quick results medium cell and application | |
| CN101948745B (en) | Artificial mouth simulating device | |
| CN103884825B (en) | A kind of evaluation of pesticide effectiveness method of preventing and treating sheath and culm blight of rice medicine | |
| CN216626575U (en) | Biological agent is at soil improvement device | |
| CN104719151B (en) | The rapid propagation method that a kind of Dai Nationality hundred solves | |
| CN107828729A (en) | A kind of human lung cancer A549 derived cell strains and its preparation method and application | |
| CN101948746B (en) | Artificial mouth simulating system device | |
| CN105647862A (en) | Bioreactor, stirring paddle thereof and method for culturing NK cells by using bioreactor | |
| CN206371271U (en) | A kind of salt-soda soil wheat ditch broadcasts analogue means | |
| CN209292364U (en) | A kind of cell incubator | |
| CN217337067U (en) | Thick myzus persicae bee detection device that lays eggs | |
| CN104293734A (en) | Preparation method of human gamma delta T cell | |
| CN104419676A (en) | Construction of down's syndrome cell model and cell bank of down's syndrome cell by employing hTERT gene recombination | |
| CN210314219U (en) | Microbial detection incubator | |
| CN219907691U (en) | Novel embryo culture dish | |
| CN101892155A (en) | Culture apparatus special for in-situ culture of amniotic fluid/chorionic cells and application thereof | |
| CN218372218U (en) | Probiotics inoculation breeding device | |
| CN201793580U (en) | Culturing device special for amniotic fluid/chorionic cell in-situ cultivation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |