CN103289898B - Elliptical orbit type cell culture oscillator - Google Patents
Elliptical orbit type cell culture oscillator Download PDFInfo
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- CN103289898B CN103289898B CN201310272693.5A CN201310272693A CN103289898B CN 103289898 B CN103289898 B CN 103289898B CN 201310272693 A CN201310272693 A CN 201310272693A CN 103289898 B CN103289898 B CN 103289898B
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- eccentric
- elliptical orbit
- controller
- tray
- eccentric wheel
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- 238000004113 cell culture Methods 0.000 title claims abstract description 15
- 230000005540 biological transmission Effects 0.000 claims abstract description 5
- 230000007246 mechanism Effects 0.000 claims description 4
- 238000005096 rolling process Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000004114 suspension culture Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011020 pilot scale process Methods 0.000 description 1
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Abstract
The invention discloses an elliptical orbit type cell culture oscillator which comprises a main body case, eccentric devices, a stepping motor, a tray and a controller, wherein the four groups of eccentric devices are arranged in the main body case; the tray is arranged on the eccentric devices; multiple culture dishes are arranged on the tray; the stepping motor is connected with one group of eccentric device through a transmission device; the controller is connected to the stepping motor through a control line. Due to elliptical rotation of a shaking table, the centrifugal force of a mass point is changed because of change of the rotating radius, so that the liquid levels of culture solutions in the culture dishes are continuously raised in the process of rotating the tray from the short-radius position to the long-radius position; therefore, a paraboloid similar to swing is generated, and layered flow between the upper and lower layers of the culture solutions is generated; as a result, growing cells have uniform nutritional conditions and oxygen conditions in the whole cell culture process.
Description
Technical field
The present invention relates to a kind of vibrator, be specially a kind of elliptical orbit formula cell cultures vibrator.
Background technology
Cell culture technology is the important component part of biotechnology, is the basic fundamental of the each research field of biology, plays immeasurable effect in life science.Traditional cell culture processes, technique are being replaced by novel method, technique, need relevant cell cultivation apparatus equipment follow-up to support, to meet the new demand of biomass cells researchist to plant and instrument.Clothoid type cell cultures vibrator is particularly suitable on a small scale groping of pilot scale suspension culture before cultivating of cell suspension culture and biological products large scale fermentation tank or raise craft condition of scientific experiment chamber, for amplification culture is prepared.
Summary of the invention
Technical problem solved by the invention is to provide a kind of elliptical orbit formula cell cultures vibrator, to solve the problem proposing in above-mentioned background technology.
Technical problem solved by the invention realizes by the following technical solutions:
A kind of elliptical orbit formula cell cultures vibrator, comprise: main body cabinet, eccentric, stepper-motor, pallet, controller, in described main body cabinet, be provided with four groups of eccentrics, eccentric is provided with pallet, pallet is provided with some culture dish, stepper-motor connects wherein one group of eccentric by transmission mechanism, on stepper-motor, is connected with controller by control line; Described eccentric comprises: main eccentric wheel, with movable eccentric wheel, guide wheel, oval restriction track, main eccentric wheel is connected to on movable eccentric wheel, be provided with oval restriction track with movable eccentric wheel upside, be connected with guide wheel with movable eccentric wheel, the guide wheel connection of rolling on elliptical orbit.
As further scheme: described controller is provided with control panel, and control panel is provided with speed control key, indicating meter.
Compared with prior art, the invention has the beneficial effects as follows: the elliptic rotation of shaking table of the present invention, because of the variation of rotation radius, cause particle centrifugal force to change, can make nutrient solution in culturing bottle forward the continuous lifting of liquid level in the process at major radius place to from short radius at pallet, thereby produce one and be similar to the parabola waving, between the levels of nutrient solution, can produce level flows, make in the whole process of cell cultures, each grown cell all obtains uniform nutritional condition and Oxygen Condition.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is the eccentric schematic diagram in the present invention.
Embodiment
In order to make of the present inventionly to realize technique means, creation characteristic, reach object and effect is easy to understand, below in conjunction with concrete diagram, further set forth the present invention.
As shown in Fig. 1～2, elliptical orbit formula cell cultures vibrator, comprise: main body cabinet 13, eccentric, stepper-motor 10, pallet 5, controller 8, in described main body cabinet 13, be provided with four groups of eccentrics, eccentric is provided with pallet 5, pallet 5 is provided with some culture dish 6, and stepper-motor 10 connects wherein one group of eccentric by transmission mechanism 11, on stepper-motor 10, is connected with controller 8 by control line 7.
Described eccentric comprises: main eccentric wheel 1, with movable eccentric wheel 2, guide wheel 3, oval restriction track 4, main eccentric wheel 1 is connected to on movable eccentric wheel 2, be provided with oval restriction track 4 with movable eccentric wheel 2 upsides, be connected guide wheel 3 connection of rolling on ellipse restriction track 4 with guide wheel 3 with movable eccentric wheel 2.Described controller 8 is provided with control panel 9, and control panel 9 is provided with speed control key, indicating meter.
In main body cabinet 13 of the present invention, be provided with the strut member of four groups of eccentrics as pallet 5, stepper-motor 10 drives the wherein main eccentric wheel 1 of a group to rotate by transmission mechanism 11, drive and rotate with movable eccentric wheel 2, because its end has guide wheel 3 by bearing assemble, guide wheel 3 is limited on the elliptical orbit of elliptical orbit 4 plates and rolls, guide wheel 3 ends connect pallet 5 and fixture above by bearing again, and pallet 5 is done elliptic motion in company with the axle center of guide wheel 4 like this; Stepper-motor 10 is controlled rotating speed by controller 8, and control panel 9 is provided with speed and adjusts key.
Main eccentric wheel 1 drives with movable eccentric wheel 2, because guide wheel 3 is with concentric with movable eccentric wheel 2 excentric shafts and due to the restriction of ellipse restriction track 4, has eccentric axle center to rotate with movable eccentric wheel 2 because position is different around main eccentric wheel 1; The friction that guide wheel 3 produces while movement on ellipse restriction track 4 is rolled guide wheel 3 in orbit, and the axle center of guide wheel 3 is connected with the pallet of top 5, thereby realizes the elliptical trajectory of pallet 5.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (2)
1. elliptical orbit formula cell cultures vibrator, comprise: main body cabinet, eccentric, stepper-motor, pallet, controller, it is characterized in that: in described main body cabinet, be provided with four groups of eccentrics, eccentric is provided with pallet, pallet is provided with some culture dish, stepper-motor connects wherein one group of eccentric by transmission mechanism, on stepper-motor, is connected with controller by control line; Described eccentric comprises: main eccentric wheel, with movable eccentric wheel, guide wheel, oval restriction track, main eccentric wheel is connected to on movable eccentric wheel, be provided with oval restriction track with movable eccentric wheel upside, be connected with guide wheel with movable eccentric wheel, the guide wheel connection of rolling on elliptical orbit.
2. elliptical orbit formula cell cultures vibrator according to claim 1, is characterized in that: described controller is provided with control panel, and control panel is provided with speed control key, indicating meter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310272693.5A CN103289898B (en) | 2013-07-02 | 2013-07-02 | Elliptical orbit type cell culture oscillator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310272693.5A CN103289898B (en) | 2013-07-02 | 2013-07-02 | Elliptical orbit type cell culture oscillator |
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| Publication Number | Publication Date |
|---|---|
| CN103289898A CN103289898A (en) | 2013-09-11 |
| CN103289898B true CN103289898B (en) | 2014-06-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310272693.5A Active CN103289898B (en) | 2013-07-02 | 2013-07-02 | Elliptical orbit type cell culture oscillator |
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| CN (1) | CN103289898B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105537093B (en) * | 2015-12-16 | 2019-02-01 | 上海大学 | The adjustable guide tracked elliptical oscillation mechanism of eccentricity |
| CN113214970B (en) * | 2021-05-07 | 2022-05-17 | 锦州医科大学 | Self-adaptive adjustment somatic cell culture device and culture method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2534189Y (en) * | 2002-01-28 | 2003-02-05 | 方庆森 | Three-shaft synchronous driving automatic balancing rotary shaking bottle machine |
| CN101149876A (en) * | 2007-09-21 | 2008-03-26 | 北京工业大学 | Angular displacement mechanical oscillator |
| CN201343532Y (en) * | 2009-01-05 | 2009-11-11 | 杭州安普生物工程有限公司 | Rip current type oscillator |
| CN102021115A (en) * | 2009-09-21 | 2011-04-20 | 惠识瑶 | Packed bed type cell bioreactor without stirrer and method for cultivating animal cells |
| CN103008221A (en) * | 2011-09-22 | 2013-04-03 | 南京航空航天大学 | Mechanical control adjustable type precise horizontal vibration platform |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102008010780B3 (en) * | 2008-02-25 | 2009-10-15 | Sartorius Stedim Biotech Gmbh | Incubator with shaker |
-
2013
- 2013-07-02 CN CN201310272693.5A patent/CN103289898B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2534189Y (en) * | 2002-01-28 | 2003-02-05 | 方庆森 | Three-shaft synchronous driving automatic balancing rotary shaking bottle machine |
| CN101149876A (en) * | 2007-09-21 | 2008-03-26 | 北京工业大学 | Angular displacement mechanical oscillator |
| CN201343532Y (en) * | 2009-01-05 | 2009-11-11 | 杭州安普生物工程有限公司 | Rip current type oscillator |
| CN102021115A (en) * | 2009-09-21 | 2011-04-20 | 惠识瑶 | Packed bed type cell bioreactor without stirrer and method for cultivating animal cells |
| CN103008221A (en) * | 2011-09-22 | 2013-04-03 | 南京航空航天大学 | Mechanical control adjustable type precise horizontal vibration platform |
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| Publication number | Publication date |
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| CN103289898A (en) | 2013-09-11 |
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Effective date of registration: 20220425 Address after: 245000 No. 1, Furong Road, high tech Industrial Development Zone, Huangshan City, Anhui Province Patentee after: Huangshan saisibao Biotechnology Co.,Ltd. Address before: 245000 Huangshan Economic Development Zone, Anhui Province Patentee before: HUANGSHAN ZHONGXIN BIO-TECH CO.,LTD. |