CN102382764B - Rotary cell culture device - Google Patents
Rotary cell culture device Download PDFInfo
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- CN102382764B CN102382764B CN 201110086850 CN201110086850A CN102382764B CN 102382764 B CN102382764 B CN 102382764B CN 201110086850 CN201110086850 CN 201110086850 CN 201110086850 A CN201110086850 A CN 201110086850A CN 102382764 B CN102382764 B CN 102382764B
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- 238000004113 cell culture Methods 0.000 title claims abstract description 31
- 239000000463 material Substances 0.000 claims abstract description 11
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 6
- 230000009467 reduction Effects 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 101000801619 Homo sapiens Long-chain-fatty-acid-CoA ligase ACSBG1 Proteins 0.000 claims description 4
- 102100033564 Long-chain-fatty-acid-CoA ligase ACSBG1 Human genes 0.000 claims description 4
- 235000010634 bubble gum Nutrition 0.000 claims description 4
- 235000015218 chewing gum Nutrition 0.000 claims description 4
- 229940112822 chewing gum Drugs 0.000 claims description 4
- 230000008676 import Effects 0.000 claims description 2
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 claims description 2
- 238000012258 culturing Methods 0.000 abstract description 6
- 239000000725 suspension Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 239000006260 foam Substances 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 230000003068 static effect Effects 0.000 description 4
- 241000397426 Centroberyx lineatus Species 0.000 description 3
- 238000011109 contamination Methods 0.000 description 2
- 238000013019 agitation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a rotary cell culture device. The device is characterized by comprising a bracket, a drive device, a rotatable device and a culture bag, wherein the drive device is arranged on the bracket and used for driving the rotatable device to rotate; and the culture bag is of a cylindrical structure formed by a hydrophobic air-permeable material, and is detachably connected to the rotatable device and can coaxially rotates with the rotatable device. The rotary cell culture device can be used for large-scale culture of suspension cells, can obtain larger cell density on the premise of not influencing cell activity, and rolls mildly in the culturing process to prevent a culture medium from generating foam.
Description
Technical field
The present invention relates to cell culture apparatus, relate in particular a kind of rotary cell culture device for dynamic cell culture.
Background technology
At present cell cultures is divided into dynamic cultivation and static cultivation dual mode, and wherein the dynamic cell culture system has stirring-type, roller bottle formula, shaking-table several.Stirring-type is cultivated and is mainly used in suspension cell culture, perhaps in nutrient solution, add microcarrier, attached cell is attached on the microcarrier grows, long-pending to increase cell growth surface, but culturing cell under the agitation condition, the shearing force that liquid stream forms is easily to the cell injury.The roller bottle formula is cultivated the cultivation that is mainly used in attached cell, and the advantage that adopts roller bottle to cultivate is to increase the surface-area for cell attachment, and simultaneously gentle rotation is conducive to Growth of Cells.When culturing bottle rotated, cell left nutrient solution for some time, was exposed in the air, had increased the exchange of its gas, favourable Growth of Cells, but the roller bottle cost is high and the space availability is little.Shaking-table is cultivated and then to be needed the shaking table module is fitted in the CO2gas incubator, complex structure, and somewhat expensive has increased the cost of culture apparatus.
And the cultivation that present static cell cultures mode both can be used for suspension cell also can be used for the cultivation of attached cell.The mode of static cultivation cell is static owing to nutrient solution in cell growth process, the unbalanced propagation that limits thus cell of distribution of nutrient element, so cell density is not high, is difficult to large scale culturing.
Summary of the invention
The object of the present invention is to provide a kind of rotary cell culture device for dynamic cell culture, existing roller bottle formula cell culture apparatus is improved, can be used for the cultivation of large-scale suspension cell, under the prerequisite that does not affect cytoactive, not only can obtain larger cell density, and it is gentle to roll in culturing process, and substratum can not produce foam.
The present invention adopts following technical scheme to realize above-mentioned purpose: a kind of rotary cell culture device, it is characterized in that, this culture apparatus comprises support, drive unit, rotatable means and culture bag, this drive unit is rack-mount, being used for driving rotatable means rotates, the cylinder-like structure of this culture bag for being made of the hydrophobic, air-permeability material, this culture bag is dismountable to be connected on the described rotatable means and with its coaxial rotation.
As further specifying of such scheme:
Preferably, described drive unit includes motor, the electric control gear that is used for this motor of control, the deceleration device that is connected with motor, this electric control gear includes the speed governing knob that is located on the support and the scale card corresponding with this speed governing knob, this deceleration device is train of reduction gears, the motion-work wheel of this train of reduction gears is fixedly connected with the rotation axis of this electric motor is coaxial, and the follow-up pulley of this train of reduction gears is connected to drive this rotatable means and rotates with rotatable means.
Preferably, described rotatable means is the chewing gum and bubble gum that is connected on the support, this cylinder is the cylindrical structure of end opening the other end sealing, and this cylinder is supported on the support by the fulcrum wheel along continuous straight runs that is arranged on the support, and the opening end of this cylinder outwardly.
Preferably, described fulcrum wheel is for being arranged on one group of above roller on the support, and every group of roller all has two, and this roller can roll with cylinder outer wall and be connected.
Preferably, the blind end of described cylinder is fixedly connected with the wheel shaft of described follow-up pulley, and this wheel shaft is coaxial with the axis of chewing gum and bubble gum, rotates to drive this cylinder.
Preferably, described cylinder is provided with gear ring near the outer rim of blind end, and the follow-up pulley of this train of reduction gears and the engagement of this gear ring are rotated to drive this cylinder.
Preferably, the cylindrical seal barrel structure of described culture bag for being made of the hydrophobic, air-permeability material is provided with material import and export, dismountable coaxial being fixedly connected in the cylinder of sealing cylinder in the end of sealing cylinder.
Preferably, the mode of connection of described culture bag and cylinder for peg graft, articulate, clamping or bonding, described being plugged in the culture bag periphery arranges cutting along axis direction, and transplants a cutting corresponding slot at drum inner surface setting and this, cooperated with this slot by this cutting to consist of grafting.
Preferably, also be provided with on the described support for the anchor that supports the follow-up pulley wheel shaft.
The beneficial effect that the present invention adopts above technical scheme to reach is:
1. culture apparatus of the present invention is simple in structure, easy to operate, can be used for large-scale suspension cell culture, leave standstill to cultivate with traditional suspension cell and compare, larger cell density can be obtained and cytoactive can not be had influence on, and it is gentle to roll in the culturing process, damage to cell is significantly less than the cultivation of impeller stirring-type, and substratum can not produce foam.
2. the culture bag of culture apparatus of the present invention is that the hydrophobic, air-permeability material consists of, and can be disposable use, can not only reduce the treating processes of culture vessel, effectively avoids microbial contamination and cell crossed contamination, and with low cost.
Description of drawings
Fig. 1 is the elevational schematic view of cell culture apparatus of the present invention;
Fig. 2 is the roller frame synoptic diagram of cell culture apparatus of the present invention;
Fig. 3 is the culture bag structural representation of cell culture apparatus of the present invention;
Fig. 4 is the cylinder A-A sectional view of cell culture apparatus of the present invention;
Fig. 5 is the culture bag B-B sectional view of cell culture apparatus of the present invention.
Description of reference numerals:
The 1-cylinder, 2-support, 3-base plate, 4-scale card, 5-speed governing knob, 6-motor, 7-motion-work wheel, 8-follow-up pulley, 9-anchor, 10-gear shaft, 11-roller, 12-culture bag, 13-material inlet/outlet pipe, 14-swallow tail, 15-dovetail-indent.
Embodiment
For further setting forth structure and function of the present invention, below in conjunction with accompanying drawing and preferred embodiment the present invention is elaborated, but be to be understood that protection scope of the present invention is not subjected to the restriction of embodiment.
Fig. 1 is the elevational schematic view of cell culture apparatus of the present invention, and as shown in FIG., this culture apparatus comprises support 2, drive unit, cylinder 1 and culture bag 12.
This support 2 includes the base plate 3 of a rectangle, and above-mentioned drive unit just is arranged on this base plate 3 left sides, on the right side of base plate 3, is provided with one group of fulcrum wheel, and this group fulcrum wheel includes two rollers 11, in order to back-up roller 1;
This drive unit is arranged on base plate 3 left sides, include motor 6, the electric control gear that is used for this motor 6 of control, the deceleration device that is connected with motor 6, this electric control gear includes speed governing knob 5 and the scale card 4 corresponding with this speed governing knob 5, this deceleration device is the double-reduction gear group, the motion-work wheel 7 of this double-reduction gear group is fixedly connected with the rotation axis of this electric motor is coaxial, the follow-up pulley 8 of this double-reduction gear group is by a rooted tooth wheel shaft 10 and cylinder 1 coaxial being fixedly connected with, being used for driving cylinder 1 rotates, on base plate 3, also be provided with an anchor 9, be used for supporting this gear shaft 10.
Fig. 2, Fig. 4 are respectively structural representation and the A-A sectional view of cylinder 1 of the present invention, as shown in FIG., this cylinder 1 is the stainless steel cylindrical structure of end opening the other end sealing, this cylinder 1 is supported on the base plate 3 by roller 11 along continuous straight runs are set, and the opening end of this cylinder 1 is towards the right side, at this cylinder 1 internal surface, along four dovetail-indents 15 of its axis direction, these four dovetail-indents 15 are used for fixedly culture bag 12 evenly respectively at cylinder 1 internal surface.
Fig. 3, Fig. 5 are respectively structural representation and the B-B sectional view of culture bag 12 of the present invention, as shown in FIG., the cylindrical seal barrel structure of this culture bag 12 for being consisted of by tetrafluoroethylene, be provided with material inlet/outlet pipe 13 in the end of sealing cylinder, be used for substratum, cell access or derivation, in culture bag 12 peripheries swallow tail 14 along axis direction is set, this swallow tail 14 is corresponding one by one with the dovetail-indent 15 of cylinder 1 internal surface, is used for inserting fixedly culture bag 12 of dovetail-indent 15.
During use, by the material inlet/outlet pipe 13 on the culture bag 12 substratum and cell are accessed batch cultur bag 12 first, then the swallow tail 14 on the disposable cell culture bags 12 is touched in the dovetail-indent 15 of cylinder 1 correspondence position and be fixed, and then the velocity of rotation by speed governing knob 5 regulating electric machines 6, motor 6 drives cylinder 1 by motion-work wheel 7 and follow-up pulley 8 and does slowly rotation, cylinder 1 drives the disposable cell culture bags 12 that is plugged on its inside and rolls together, thereby realizes the dynamic cultivation of cell.
Only for one of preferred embodiment of the present invention, can not limit to the present invention with this shown in the above embodiment of the present invention and the accompanying drawing, under the condition that does not break away from marrow of the present invention, any change that those skilled in the art do all belongs to protection scope of the present invention.
Claims (7)
1. a rotary cell culture device is characterized in that, this culture apparatus comprises support, drive unit, rotatable means and culture bag:
This drive unit is rack-mount, is used for driving rotatable means and rotates, and described drive unit includes motor, the electric control gear that is used for this motor of control, the deceleration device that is connected with motor; This deceleration device is train of reduction gears, and the motion-work wheel of this train of reduction gears is fixedly connected with the rotation axis of this electric motor is coaxial, and the follow-up pulley of this train of reduction gears is connected to drive this rotatable means and rotates with rotatable means;
This rotatable means is the chewing gum and bubble gum that is connected on the support, and this cylinder is the cylindrical structure of end opening the other end sealing, and this cylinder is supported on the support by the fulcrum wheel along continuous straight runs that is arranged on the support, and the opening end of this cylinder outwardly; Described fulcrum wheel is for being arranged on one group of above roller on the support, and every group of roller all has two, and this roller can roll with cylinder outer wall and be connected;
The cylindrical seal barrel structure of this culture bag for being made of the hydrophobic, air-permeability material is provided with material import and export in the end of sealing cylinder, dismountable coaxial being fixedly connected in the cylinder of sealing cylinder, and with its coaxial rotation.
2. rotary cell culture device according to claim 1 is characterized in that, this electric control gear includes the speed governing knob that is located on the support and the scale card corresponding with this speed governing knob.
3. rotary cell culture device according to claim 1 is characterized in that, the blind end of described cylinder is fixedly connected with the wheel shaft of described follow-up pulley, and this wheel shaft is coaxial with the axis of chewing gum and bubble gum, rotates to drive this cylinder.
4. rotary cell culture device according to claim 1 is characterized in that, described cylinder is provided with gear ring near the outer rim of blind end, and the follow-up pulley of this train of reduction gears and the engagement of this gear ring are rotated to drive this cylinder.
5. rotary cell culture device according to claim 1, it is characterized in that, the mode of connection of described culture bag and cylinder for peg graft, articulate, clamping or bonding, described being plugged in the culture bag periphery arranges cutting along axis direction, and transplant a cutting corresponding slot at drum inner surface setting and this, cooperated with this slot by this cutting to consist of grafting.
6. rotary cell culture device according to claim 1 is characterized in that, also is provided with on the described support for the anchor that supports the follow-up pulley wheel shaft.
7. rotary cell culture device according to claim 1 is characterized in that, described hydrophobic, air-permeability material is tetrafluoroethylene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110086850 CN102382764B (en) | 2010-08-25 | 2011-04-07 | Rotary cell culture device |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020505659 | 2010-08-25 | ||
| CN201020505659.X | 2010-08-25 | ||
| CN 201110086850 CN102382764B (en) | 2010-08-25 | 2011-04-07 | Rotary cell culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102382764A CN102382764A (en) | 2012-03-21 |
| CN102382764B true CN102382764B (en) | 2013-05-29 |
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|---|---|---|---|
| CN 201110086850 Active CN102382764B (en) | 2010-08-25 | 2011-04-07 | Rotary cell culture device |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106047707B (en) * | 2016-08-03 | 2018-11-13 | 广州赛泊泰生物技术有限公司 | Adherent/floating type cell culture unit, device, system and method |
| CN110157617B (en) * | 2019-04-29 | 2021-07-16 | 重庆大学附属肿瘤医院 | Cervical cancer cell three-dimensional culture device and application thereof |
| CN112457987B (en) * | 2020-12-11 | 2024-09-10 | 广州瑞铂茵健康科技有限公司 | Stirring type suspension cell culture device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1498960A (en) * | 2002-10-31 | 2004-05-26 | 惠普开发有限公司 | Cell culture device |
| CN1531589A (en) * | 2001-07-09 | 2004-09-22 | ��������װҩ���˽�˱�����˾ | Disposable vessel |
| CN1740314A (en) * | 2005-07-21 | 2006-03-01 | 高春平 | Continuous filling automatic cell culture system |
-
2011
- 2011-04-07 CN CN 201110086850 patent/CN102382764B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1531589A (en) * | 2001-07-09 | 2004-09-22 | ��������װҩ���˽�˱�����˾ | Disposable vessel |
| CN1498960A (en) * | 2002-10-31 | 2004-05-26 | 惠普开发有限公司 | Cell culture device |
| CN1740314A (en) * | 2005-07-21 | 2006-03-01 | 高春平 | Continuous filling automatic cell culture system |
Non-Patent Citations (2)
| Title |
|---|
| 体外细胞基底拉伸加载装置的研制与应用;徐东等;《医疗卫生装备》;20080430;第29卷(第4期);第3-4页，第8页 * |
| 徐东等.体外细胞基底拉伸加载装置的研制与应用.《医疗卫生装备》.2008,第29卷(第4期),第3-4页，第8页. |
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Owner name: BEIJING DOING TIMES BIOMEDICAL TECHNOLOGY CO., LTD Free format text: FORMER OWNER: LI GANGYI Effective date: 20141231 |
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Effective date of registration: 20141231 Address after: 100021, room 5, building 11, building 7, 529 North Road, pine Elm Road, Beijing, Chaoyang District Patentee after: BEIJING DOING-TIMES BIOMEDICAL TECHNOLOGY Co.,Ltd. Address before: 100021, room 318, building 2, North Elm Road, Beijing, Chaoyang District Patentee before: Li Gangyi |
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Denomination of invention: A rotating cell culture device Effective date of registration: 20210901 Granted publication date: 20130529 Pledgee: Ningbo Kuanjie Tibetan jade enterprise management partnership (L.P.) Pledgor: BEIJING DOING-TIMES BIOMEDICAL TECHNOLOGY Co.,Ltd. Registration number: Y2021990000799 |
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Date of cancellation: 20230904 Granted publication date: 20130529 Pledgee: Ningbo Kuanjie Tibetan jade enterprise management partnership (L.P.) Pledgor: BEIJING DOING-TIMES BIOMEDICAL TECHNOLOGY Co.,Ltd. Registration number: Y2021990000799 |
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