CN218879938U - Cell perfusion culture system - Google Patents

Abstract

The utility model is used in the technical field of bioreactors, in particular to a cell perfusion culture system, which comprises a culture device, a shaking device and a holder, wherein the holder is arranged on the shaking device; a first container provided on one side of the culture apparatus; one end of the first pipeline is communicated with the first container, the other end of the first pipeline is connected with the incubator, and a first delivery pump and a first control valve are arranged on the first pipeline; the culture medium container is arranged on one side of the culture device, and the stirring device is arranged on the tank body; one end of the second pipeline is communicated with the other end of the culture medium container and is connected with the first pipeline, and a second control valve is arranged on the second pipeline. The risk of contamination of such culture systems is lower.

Description

Cell perfusion culture system

Technical Field

The utility model is used for bioreactor technical field especially relates to a cell perfusion culture system.

Background

At present, the adherent cells are cultured in vitro by two-dimensional static culture in an open or semi-open container, such as a culture dish, a culture bottle, a multi-layer culture bottle, a cell factory and the like, and the bottom surface of the culture container is usually subjected to TC treatment to facilitate the culture of the adherent cells. The cell suspension is distributed in the culture device by an operator, sufficient culture medium is added, the cells are uniformly dispersed in the container by adopting a cross or 8-shaped shaking method, then the container is placed in a cell culture box for standing, adhering and culturing, the cells are tightly attached to the bottom of the container, the materials are exchanged with the outside through a free surface, and the operator needs to regularly replace the culture medium in the container to ensure the stable growth environment of the cells.

This kind of cultivation mode has more shortcomings, has more personnel intervention steps in the cultivation process on the one hand, and complex operation and cause great pollution risk, and the cell culture scale is restricted, is not suitable for large-scale cultivation. On the other hand, the cell culture mold is single, and only cells can be attached to the bottom surface of the cell culture device for culture, so that the waste of a large culture space is caused, and the phenomenon of mass cell pollution is easily caused by the fact that the semi-open container is positioned in the same room in the culture box. In addition, the adherent static culture of the cells has low material exchange efficiency and slow growth rate, and causes a great deal of nutrient waste for maintaining a good cell growth environment, and the static culture method causes the cells to gradually lose the original characters in vivo and is far away from the natural growth state in vivo in aspects of shape, structure and function, such as gradually flattening the cells, abnormal division and losing the differentiation phenotype.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solving the inhomogeneous technical problem of cell bed board when cell perfusion is cultivateed, provide a cell perfusion culture system. The cell perfusion culture system is provided with the shaking device, can drive the cell culture container to rotate, then enables cells to be uniformly paved, can also enable adherent cells to be poured into the cell culture container in a grading manner, rotates the cell culture container by adjusting the shaking device each time, enables the cells to be adhered to each wall surface of the cell culture container, simultaneously reduces the usage amount of expensive components such as serum in unit cell culture, and saves the cost; the culture medium device is also provided with a culture medium container, and a bubble generating device and a stirring paddle for defoaming are arranged in the culture medium container, so that oxygen is increased, and cell damage caused by bubble breakage is reduced.

The utility model aims at providing a cell perfusion culture system;

in order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:

a cell perfusion culture system comprises

The culture device comprises an incubator, a shaking device, a cell culture container and a holder, wherein the shaking device and the holder are both arranged inside the incubator, the holder is arranged on the shaking device, the shaking device is used for driving the holder to rotate, and the holder is used for fixing the cell culture container;

a first container;

one end of the first pipeline is communicated with the first container, the other end of the first pipeline is connected to the incubator and is used for communicating with the cell culture container, and a first delivery pump and a first control valve are arranged on the first pipeline;

a culture medium container comprising a tank and a stirring device, the stirring device being mounted within the tank;

and one end of the second pipeline is communicated with the culture medium container, the other end of the second pipeline is communicated with the cell culture container, a second control valve is arranged on the second pipeline, and a delivery pump is arranged on the second pipeline or between the second pipeline and the cell culture container.

The cell perfusion culture system at least has the following beneficial effects: the culture system shakes the cell culture container in the incubator through the shaking device, so that the operation is not required to be interfered by personnel, the operation is simple, and the risk of cell pollution caused by human is lower.

As the utility model discloses preferred technical scheme, among the cell perfusion culture system the device that rocks includes support, a drive arrangement and rocks the frame, the cloud platform is installed rock on the frame, rock the frame and rotate to install on the support, rock the connection of frame the power take off end of a drive arrangement, a drive arrangement is used for driving it is rotatory as the axle center with the horizontal axis that rocks the frame. Thereby realizing that the shaking frame drives the cell culture container to rotate, and then playing a role in shaking the culture solution in the cell culture container. In addition, the adherent cells are poured into the cell culture container in a plurality of times, and the cell culture container is rotated by adjusting the shaking frame every time, so that the cells are attached to each wall surface of the cell culture container.

Preferably, the shaking frame comprises a fixed ring and a rotating ring, the rotating ring is rotatably mounted in the fixed ring, and the rotating ring is detachably connected with the holder;

the power output end of the first driving device is connected with the fixing ring of the shaking frame. Thereby realizing that the shaking frame drives the cell culture container to rotate, and then playing a role in shaking the culture solution in the culture container.

And a second driving device is arranged on the shaking frame and used for driving the rotating ring to rotate. Thereby realizing the function of horizontally shaking the culture solution in the cell culture container by the shaking frame.

Preferably, a connecting block is installed on the rotating ring, a connecting plate is installed on the holder, and the connecting plate is detachably connected with the connecting block through a fastener. Thus, the disassembly, assembly and replacement between the rotating ring and the holder are facilitated.

Preferably, the connecting plate is provided with an adjusting groove, and the fastener penetrates through the adjusting groove and is in threaded connection with the connecting block. The relative height of the cradle head can be conveniently adjusted.

As the preferred technical scheme of the utility model, cell perfusion culture system still includes the second container, the second container with be equipped with the third pipeline between the culture apparatus, the one end intercommunication of third pipeline the second container, the other end of third pipeline is connected be used for communicateing the cell culture container on the incubator, be equipped with second delivery pump and third control valve on the third pipeline. The liquid in the cell culture vessel can be drained through a third conduit to a second vessel for collection.

Preferably, a fourth pipeline is arranged between the culture medium container and the cell culture container, one end of the fourth pipeline is communicated with the culture medium container, the other end of the fourth pipeline is communicated with the cell culture container, a fourth control valve is arranged on the fourth pipeline, and a delivery pump is arranged on the fourth pipeline or between the fourth pipeline and the cell culture container.

As the preferred technical scheme of the utility model, the culture medium container still includes bubble generating device, and bubble generating device installs in jar body bottom. The bubble generation device generates oxygen-containing bubbles to supplement the oxygen content in the culture medium.

Preferably, the culture medium container further comprises a pressure relief device, and the pressure relief device is mounted on the tank body. The pressure relief device can ensure the pressure in the tank body to be stable.

Wherein, preferentially, culture medium container still includes agitating unit, and agitating unit includes third power component, transfer line, first stirring rake and second stirring rake, the third power component is installed jar on the body, the transfer line is connected the power take off end of third power component, the transfer line stretches into jar in the body, first stirring rake with the second stirring rake is all installed on the transfer line, first stirring rake is located between power take off end and the second stirring rake of third power component. First stirring rake can be broken the bubble or decompose into the microbubble through the stirring, reduces the production of foam in the liquid, reduces the cell damage that the bubble broke and caused then, and the oxygen dissolved quantity of culture medium in the multiplicable jar body of second stirring rake through the stirring.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:

FIG. 1 is a schematic view of an embodiment of the present invention;

FIG. 2 is a schematic view showing a structure of a culture apparatus according to an embodiment of the present invention;

FIG. 3 is a schematic view of the connection between the rocking device and the support in an embodiment of the present invention;

FIG. 4 is a schematic view of a rocking device according to an embodiment of the present invention;

FIG. 5 is a schematic view of a rocking device according to an embodiment of the present invention;

FIG. 6 is a schematic view showing the internal structure of a medium container according to an embodiment of the present invention.

The attached drawings are marked as follows: 100. a culture device; 110. an incubator; 111. a box body; 112. a box cover; 113. a liquid inlet channel; 114. a liquid outlet channel; 115. a control panel; 120. a shaking device; 121. a support; 123. a first driving device; 1230. a first power component; 1231. a first synchronization belt; 1232. a first driven wheel; 124. shaking the frame; 1240. a fixing ring; 1241. a rotating ring; 1242. a tooth-shaped structure; 1243. connecting blocks; 125. a second driving device; 1250. a second power component; 1251. a second drive wheel; 1252. a tension wheel; 130. a holder; 131. a connecting plate; 132. a first plate member; 133. a second plate member; 134. an adjustment groove; 200. a first container; 300. a first conduit; 301. a first delivery pump; 302. a first control valve; 400. a culture medium container; 401. a third power component; 402. a first stirring paddle; 403. a transmission rod; 404. a second stirring paddle; 500. a cell culture vessel; 600. a second pipe; 601. a second control valve; 700. a second container; 800. a third pipeline; 801. a second delivery pump; 802. a third control valve; 900. a fourth conduit; 902. a fourth control valve.

Detailed Description

The conception and the resulting technical effects of the present invention will be described clearly and completely with reference to the following embodiments, so that the objects, features and effects of the present invention can be fully understood. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and other embodiments obtained by those skilled in the art without inventive labor based on the embodiments of the present invention all belong to the protection scope of the present invention.

In the description of the embodiments of the present invention, if an orientation description is referred to, for example, the directions or positional relationships indicated by "upper", "lower", "front", "rear", "left", "right", etc. are based on the directions or positional relationships shown in the drawings, only for convenience of description and simplification of description, but not for indicating or implying that the indicated device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention.

In the description of the embodiments of the present invention, if a feature is referred to as being "disposed", "fixed", "connected", or "mounted" to another feature, it can be directly disposed, fixed, or connected to the other feature or indirectly disposed, fixed, connected, or mounted to the other feature. In the description of the embodiments of the present invention, if "a plurality" is referred to, it means one or more, if "a plurality" is referred to, it means two or more, if "greater than", "less than" or "more than" is referred to, it is understood that the number is not included, and if "more than", "less than" or "within" is referred to, it is understood that the number is included. References to "first" and "second" are to be understood as distinguishing technical features and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of the technical features indicated.

Referring to fig. 1 to 6, the utility model provides a cell perfusion culture system, including culture apparatus 100, first container 200, first pipeline 300, culture medium container 400 and second pipeline 600, culture apparatus 100 includes incubator 110, rocking device 120, cell culture container 500 and cloud platform 130, rocking device 120 and cloud platform 130 all establish inside incubator 110, cloud platform 130 installs on rocking device 120, rocking device 120 is used for driving cloud platform 130 to rotate, be used for fixed cell culture container 500 on the cloud platform 130, first container 200 is established in one side of culture apparatus 100, the one end of first pipeline 300 communicates first container 200, the other end of first pipeline 300 is connected and is used for communicating cell culture container 500 on incubator 110, be equipped with first delivery pump 301 and first control valve 302 on the first pipeline 300, culture medium container 400 establishes in one side of culture apparatus 100, culture medium container 400 includes the jar body and agitating unit 410, agitating unit 410 installs in the jar body, the one end of second pipeline 600 communicates culture medium container 400, the other end of second pipeline 600 connects between first delivery pump 301 and first control valve 302, be equipped with second pipeline 601 on the second pipeline 600.

In some embodiments, second conduit 600 directly connects cell culture container 500 to medium container 400, i.e., one end of second conduit 600 is connected to medium container 400, the other end is connected to cell culture container 500, second control valve 601 is disposed on second conduit 600, and a transfer pump is disposed on second conduit 600 or between second conduit 600 and cell culture container 500.

This culture system is operated without human intervention by shaking the cell culture vessel 500 in the culture chamber 110 by the shaking means 120, and is simple to operate and causes less risk of contamination by human.

In some embodiments, the cell perfusion culture system further comprises a second container 700, a third pipeline 800 is disposed between the second container 700 and the culture apparatus 100, one end of the third pipeline 800 is communicated with the second container 700, the other end of the third pipeline 800 is connected to the culture tank 110 for communicating with the cell culture container 500, and a second delivery pump 801 and a third control valve 802 are disposed on the third pipeline 800.

In some embodiments, a fourth pipeline 900 is disposed between the medium container 400 and the third pipeline 800, one end of the fourth pipeline 900 is communicated with the medium container 400, the other end of the fourth pipeline 900 is connected to the third pipeline 800 between the second transfer pump 801 and the third control valve 802, and a fourth control valve 902 is disposed on the fourth pipeline 900.

Further, a fourth pipe 900 may be provided between the medium container 400 and the cell culture container 500, one end of the fourth pipe 900 may be communicated with the medium container 400, the other end may be communicated with the cell culture container 500, a fourth control valve 902 may be provided on the fourth pipe 900, and a transfer pump may be provided on the fourth pipe 900 or between the fourth pipe 900 and the cell culture container 500.

As shown in fig. 2, the incubator 110 includes a case 111 and a cover 112, wherein an opening is formed at one side of the case 111, the cover 112 is hinged to the case 111 to open or close the opening side of the case 111, two inlet channels 113 are formed at the left side of the case 111, and two outlet channels 114 are formed at the right side of the case 111.

One end of the first pipe 300 is connected to one of the liquid inlet channels 113 on the left side of the box 111 and then communicated with the cell culture container 500 through a filling pipe, and one end of the third pipe 800 is connected to one of the liquid outlet channels 114 on the right side of the box 111 and then communicated with the cell culture container 500 through a filling pipe. Wherein the perfusion pipeline is a flexible hose; or the first pipe 300 and the third pipe 800 are both flexible pipes, which are respectively connected with the cell culture container 500 through the liquid inlet channel 113 and the liquid outlet channel 114.

The shaking device 120 comprises a bracket 121, a first driving device 123 and a shaking frame 124, the first driving device 123 and the bracket 121 are both arranged inside the box body 111, the pan/tilt head 130 is mounted on the shaking frame 124, the shaking frame 124 is rotatably mounted on the bracket 121 and located in an inner cavity of the box body 111, the shaking frame 124 is connected with a power output end of the first driving device 123, and the first driving device 123 is used for driving the shaking frame 124 to rotate around a horizontal shaft of the shaking frame 124 (for example, the X-axis of fig. 4 is used as an axis). Can realize rocking like this that frame 124 drives cell culture container 500 and rotate, then play the swing of culture solution in cell culture container 500 and rock the effect, in addition, let the adherent cell pour into cell culture container 500 into in grades, rock frame 124 and rotate cell culture container 500 through the adjustment at every turn, let the cell adhere to the wall on each wall of cell culture container 500.

Specifically, the bracket 121 is of a U-shaped structure, the bracket 121 is hidden and installed inside the box body 111, the first driving device 123 is hidden and fixed inside the box body 111, the two ends of the shaking frame 124 are provided with the roller shafts, and the roller shafts penetrate through the inner side wall of the box body 111 and are rotatably connected with the bracket 121.

The first driving device 123 includes a first power component 1230, a first driving wheel, a first driven wheel 1232 and a first synchronous belt 1231, the first power component 1230 is fixed inside the box 111, the first driving wheel is installed at the power output end of the first power component 1230, the center of a circle of the first driven wheel 1232 is fixedly connected with the roller shaft at one end of the shaking frame 124, and the first driving wheel and the first driven wheel 1232 are connected with the first synchronous belt 1231. In the operation process, the first power member 1230 drives the first driving wheel to rotate, and the first driving wheel drives the first driven wheel 1232 to rotate through the first synchronous belt 1231, so that the shaking frame 124 is turned around the X axis (as shown in fig. 4, the X axis is a rotation axis), and since the holder 130 is mounted on the shaking frame 124, the cell culture container 500 mounted on the holder 130 is also turned around, so as to shake the culture solution in the cell culture container 500.

In some embodiments, the swaying frame 124 includes a fixing ring 1240 and a rotating ring 1241, the rotating ring 1241 is rotatably installed inside the fixing ring 1240, the rotating ring 1241 is detachably connected to the pan/tilt head 130, and the swaying frame 124 is further provided with a second driving device 125, wherein the second driving device 125 is used for driving the rotating ring 1241 to rotate.

Specifically, the diameter of the rotating ring 1241 is smaller than that of the fixing ring 1240, a first annular groove is formed in the outer wall of the rotating ring 1241, a second annular groove is formed in the inner wall of the fixing ring 1240, the rotating ring 1241 is arranged inside the fixing ring 1240, a ball is arranged between the first annular groove and the second annular groove, so that the rotating ring 1241 is rotatably installed inside the fixing ring 1240, the rotating ring 1241 is higher than the fixing ring 1240, and a tooth-shaped structure 1242 is arranged on the outer wall of the rotating ring 1241.

In some embodiments, the second driving device 125 includes a second power component 1250, a second driving wheel 1251, two tensioning wheels 1252, a second synchronous belt and a base 1253, the base 1253 is mounted on the fixing ring 1240, the second power component 1250 is fixed on the base 1253, the second driving wheel 1251 is mounted at the power output end of the second power component 1250, the two tensioning wheels 1252 are respectively disposed between the toothed structure 1242 of the rotating ring 1241 and the second driving wheel 1251, one end of the second synchronous belt is sleeved on the second driving wheel 1251, the other end of the second synchronous belt is sleeved on the toothed structure 1242 of the rotating ring 1241, the two tensioning wheels 1252 are respectively pressed on two sides of the second synchronous belt, and the inner wall of the rotating ring 1241 is detachably connected to the pan tilt head 130 through the connecting block 1243 and the connecting plate 131. In the working process, the second driving wheel 1251 is driven by the second power component 1250 to rotate, and the second driving wheel 1251 drives the rotating ring 1241 to rotate through the second synchronous belt, so as to shake the cell culture container 500 on the holder 130.

The two ends of the outer wall of the fixing ring 1240, which is symmetrical around the center of the ring, are provided with roll shafts, and the roll shafts penetrate through the inner side wall of the box body 111 to be rotatably connected with the bracket 121, so that the horizontal shaft of the shaking frame 124 is used as an axis to rotate.

In some embodiments, the inner wall of the spin ring 1241 is removably attached to the pan head 130 via a connection block 1243 and a connection plate 131.

Specifically, three connecting blocks 1243 are provided on the spin ring 1241, the three connecting blocks 1243 are arranged at intervals in the circumferential direction of the spin ring 1241, three connecting plates 131 are provided, the connecting plate 131 includes a first plate 132 and a second plate 133, the first plate 132 is connected to the connecting blocks 1243 by a fastener, the first plate 132 is vertically connected to one end of the second plate 133, the first plate 132 is connected to the connecting blocks 1243 by a fastener, and the second plate 133 is connected to the bottom of the pan/tilt head 130.

In order to facilitate the adjustment of the height of the holder 130, in some embodiments, the first plate 132 of the connecting plate 131 is provided with an adjusting slot 134, the connecting block 1243 is provided with a threaded hole, and the fastening member is a fastening screw, and the fastening screw is threaded through the threaded hole of the connecting block 1243 and tightly penetrates through the adjusting slot 134. When the height of the holder 130 needs to be adjusted, the fasteners (fastening screws) are loosened, then the holder 130 and the connecting plate 131 are adjusted along the extending direction of the adjusting grooves 134, and the fasteners are tightened after the adjustment is proper.

In some embodiments, media container 400 further includes a bubble generation device mounted at the bottom of the tank.

Preferably, the stirring device 410 comprises a third power part 401, a transmission rod 403, a first stirring paddle 402 and a second stirring paddle 404, the third power part 401 is mounted on the tank, one end of the transmission rod 403 is connected to the power output end of the third power part 401, the other end of the transmission rod 403 extends into the tank, and the first stirring paddle 402 and the second stirring paddle 404 are both mounted on the transmission rod 403, and the first stirring paddle 402 is located between the power output end of the third power part 401 and the second stirring paddle 404. The second stirring paddle 404 near the bottom of the tank can increase the oxygen dissolved amount of the culture medium in the tank by stirring; the first stirring paddle 402 far away from the bottom of the tank body can break or decompose the bubbles into small bubbles through stirring, so that the generation of foams in the liquid is reduced, and then, the cell damage caused by the bubble breakage is reduced.

In some embodiments, media container 400 further includes a pressure relief device mounted on the canister. Pressure relief device can guarantee that jar internal pressure is stable.

The system of this embodiment further includes a control system, the control system includes a control panel 115 and a plurality of detection heads, each detection head all is connected with control panel 115 control, all be equipped with the detection head on first pipeline 300, second pipeline 600, third pipeline 800 and the fourth pipeline 900, be used for monitoring in the pipeline various indexes of carrying solution, control panel 115 is connected with medium container 400.

In addition, the first driving device 123 and the second driving device 125 are both in control connection with the control panel 115, and the control panel 115 can control the start and stop of the first power component 1230 and the second power component 1250.

The cell perfusion culture system is combined to implement cell perfusion culture, and the method comprises the following steps:

the whole perfusion culture system is pre-filled by sterile or normal saline permeating solution after being integrally sterilized;

opening the incubator 110, fixing the cell culture container to the holder 130, and connecting the cell culture container 500 with the first pipe 300 and the third pipe 800;

injecting the prepared cell suspension into the first container 200, and opening the first transfer pump 301 to inject the cell suspension in the first container 200 into the cell culture container 500;

the first driving device 123 drives the shaking frame 124 to turn over by taking the horizontal shaft of the shaking frame as the axis, and the second driving device 125 drives the rotating ring 1241 to rotate so as to shake the cell suspension in the cell culture container 500 and fix the angle for fixing surface adhesion;

the second transfer pump 801 is turned on to discharge the liquid in the cell culture container 500 into the second container 700 through the third pipe 800;

a medium vessel 400 in which a medium is contained is filled into the cell culture vessel 500 by a first transfer pump 301, and then the medium is transferred back to the medium vessel 400 by a second transfer pump 801;

the medium in the medium container 400 is stirred by the stirring device 410;

after the cell culture reaches the standard, the program is closed, the pipeline is dismantled, and the cell culture container 500 is taken out for sealing or use after the interface is sealed.

The specific process is as follows:

the whole perfusion culture system is sterilized integrally, the incubator 110 is opened after the perfusion culture system is pre-filled with sterile PBS or normal saline osmotic solution, the cell culture container 500 is fixed on the holder 130, a perfusion pipeline is connected between the cell culture container 500 and the liquid inlet channel 113 on the left side of the incubator 110, a perfusion pipeline is connected between the cell culture container 500 and the liquid outlet channel 114 on the right side of the incubator 110, and the incubator 110 is closed.

Injecting the prepared cell suspension into the first container 200, opening the first control valve 302, closing the second control valve 601, the third control valve 802 and the fourth control valve 902, opening the first delivery pump 301 for cell suspension perfusion, after the cell suspension fills the whole cell culture container 500, closing the first delivery pump 301 and the first control valve 302, and ending the perfusion.

The first power part 1230 drives the shaking frame 124 to turn over with the horizontal shaft of the shaking frame 124 as the axis, then the shaking frame 124 drives the cell culture container 500 to turn over and shake with the horizontal shaft of the shaking frame 124 as the axis, the second driving device 125 is started, the second driving device 125 drives the rotating ring 1241 to rotate, and the rotating ring 1241 drives the cell culture container 500 on the holder 130 to rotate and shake.

After shaking for a period of time, the first power part 1230 and the second power part 1250 are controlled by the control panel 115 to adjust the position of the cell culture container 500 in the incubator 110, and the cell culture container is left standing for 1-4 hours to wait for the adherent adhesion of the cells, after the cells are adhered, the third control valve 802 is opened, the second delivery pump 801 is turned on, and the liquid in the cell culture container 500 is discharged into the second container 700 through the third pipeline 800 (or the first delivery pump 301 is turned on to deliver the liquid in the cell culture container 500 back into the first container 200).

If cells are allowed to adhere to the wall surfaces of the cell culture container 500, the cells to be adhered are poured into the cell culture container 500 in several times, and the above operations are repeated, and the shaking frame 124 is adjusted and rotated each time to rotate the cell culture container 500, so that the cells are allowed to adhere to the wall surfaces of the cell culture container 500.

The medium container 400 is filled with medium, the first control valve 302 is closed, the second control valve 601, the fourth control valve 902, the first transfer pump 301, and the second transfer pump 801 are opened, the medium is filled into the cell culture container 500 through the second pipe 600 to culture the cells, and then the medium is transported back to the medium container 400 through the fourth pipe 900.

Monitoring each index of culture medium in the perfusion process through the control system, control panel 115 can control culture medium container 400 according to the setting, in order to rectify the culture medium of culture medium container 400, for example when the oxygen content is less than the settlement scope, control panel 115 controls the bubble generating device to start, the bubble generating device produces the oxygen content in oxygen-containing bubble supply culture medium, the stirring through first stirring rake 402 is through the thin slice shearing of paddle and destroys the surface tension of bubble, make its breakage thereby reduce the production of foam in the liquid, and then reduce the cell damage that the bubble broke and cause, the stirring through second stirring rake 404 is through the collision of paddle and bubble with big bubble breakage into the small bubble, increase the specific surface area of bubble and liquid contact, thereby increase the dissolved amount of oxygen in the culture medium, the internal pressure of jar can carry out the pressure release processing through pressure release device, guarantee jar internal pressure stability. The pressure in the tank body can be released through the pressure release device, so that the pressure in the tank is stable.

After the cell culture reaches the standard, the program is closed, the pipeline is dismantled, and the cell culture container 500 is taken out for sealing or use after the interface is sealed.

The embodiments of the present invention have been described in detail with reference to the drawings, but the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art. Furthermore, the embodiments of the present invention and features of the embodiments may be combined with each other without conflict.

Claims (10)

1. A cell perfusion culture system is characterized in that: comprises that

The culture device comprises an incubator, a shaking device, a cell culture container and a holder, wherein the shaking device and the holder are both arranged inside the incubator, the holder is arranged on the shaking device, the shaking device is used for driving the holder to rotate, and the holder is used for fixing the cell culture container;

a first container;

one end of the first pipeline is communicated with the first container, the other end of the first pipeline is connected to the incubator and is used for communicating with the cell culture container, and a first delivery pump and a first control valve are arranged on the first pipeline;

a culture medium container comprising a tank and a stirring device, the stirring device being mounted within the tank;

and one end of the second pipeline is communicated with the culture medium container, the other end of the second pipeline is communicated with the cell culture container, a second control valve is arranged on the second pipeline, and a delivery pump is arranged on the second pipeline or between the second pipeline and the cell culture container.

2. The cell perfusion culture system of claim 1, wherein: the device that rocks includes support, a drive arrangement and rocks the frame, the cloud platform is installed rock on the frame, rock the frame and rotate and install on the support, rock the frame and connect a drive arrangement's power take off end, a drive arrangement is used for driving rock the horizontal axis that the frame used to rock the frame and rotate as the axle center.

3. The cell perfusion culture system of claim 2, wherein: the shaking frame comprises a fixing ring and a rotating ring, the rotating ring is rotatably arranged in the fixing ring, the rotating ring is detachably connected with the holder, and a second driving device is arranged on the shaking frame and used for driving the rotating ring to rotate.

4. The cell perfusion culture system of claim 1, wherein: the cell perfusion culture system further comprises a second container, a third pipeline is arranged between the second container and the culture device, one end of the third pipeline is communicated with the second container, the other end of the third pipeline is connected to the culture box and used for being communicated with the cell culture container, and a second delivery pump and a third control valve are arranged on the third pipeline.

5. The cell perfusion culture system of claim 4, wherein: a fourth pipeline is arranged between the culture medium container and the cell culture container, one end of the fourth pipeline is communicated with the culture medium container, the other end of the fourth pipeline is communicated with the cell culture container, a fourth control valve is arranged on the fourth pipeline, and a delivery pump is arranged on the fourth pipeline or between the fourth pipeline and the cell culture container.

6. The cell perfusion culture system of claim 3, wherein: the rotating ring is provided with a connecting block, the holder is provided with a connecting plate, and the connecting plate is detachably connected with the connecting block through a fastener.

7. The cell perfusion culture system of claim 6, wherein: the connecting plate is provided with an adjusting groove, and the fastener penetrates through the adjusting groove and is in threaded connection with the connecting block.

8. The cell perfusion culture system of claim 1, wherein: the culture medium container also comprises a bubble generating device, and the bubble generating device is arranged on the tank body.

9. The cell perfusion culture system of claim 8, wherein: the culture medium container further comprises a pressure relief device, and the pressure relief device is installed on the tank body.

10. The cell perfusion culture system of claim 9, wherein: agitating unit includes third power component, transfer line, first stirring rake and second stirring rake, the third power component is installed on the jar body, the transfer line is connected the power take off of third power component, the transfer line stretches into jar in the body, first stirring rake with the second stirring rake is all installed on the transfer line, first stirring rake is located the power take off of third power component with between the second stirring rake.

Images