CN203487159U - Perfusion-type bioreactor with two layers of circulating systems - Google Patents
Perfusion-type bioreactor with two layers of circulating systems Download PDFInfo
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- CN203487159U CN203487159U CN201320289836.9U CN201320289836U CN203487159U CN 203487159 U CN203487159 U CN 203487159U CN 201320289836 U CN201320289836 U CN 201320289836U CN 203487159 U CN203487159 U CN 203487159U
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Abstract
The utility model relates to the technical field of a bioreactor in medical equipment, and particularly relates to a perfusion-type bioreactor with two layers of circulating systems. The perfusion-type bioreactor with the two layers of circulating systems comprises a culture tank, an outer circulating system, an inner circulating system and a gas filling system, wherein the outer circulating system is arranged outside the culture tank, and the inner circulating system is arranged in the culture tank. The perfusion-type bioreactor with the two layers of circulating systems is used for solving the problem of the contradiction requirement on the circulating flow of a culture solution in a cell culture process, is capable of effectively improving the solubility of oxygen in a culture solution and the uniformity of oxygen distribution and effectively eliminating bubbles entering a cell culture zone and facilitates the discharging of exhaust gas in the culture solution.
Description
Technical field
The utility model relates to the bioreactor technology field in medicine equipment, more specifically, relates to a kind of filling type bioreactor of the double-deck recycle system.
Background technology
The structure of existing bio-reactor as shown in Figure 1, its function be manufacture in vitro one to similar environment in body, to accelerate the system of culturing cell, formative tissue and even organ, can be widely used in organ defect patient and substitute, build, keep or strengthen its function of organization, and the field such as bio-pharmaceuticals.Its principle is physiology, physics, the chemical property of the microenvironment of growing in vivo according to cell, utilize engineering, build in vitro a covering device, can provide with body in close microenvironment, be beneficial to cell and breed, break up in this external microenvironment, and formative tissue and even organ.
Fig. 1 is the structural representation of existing filling type bioreactor, in Fig. 1, nutrient solution in nutrient solution hold-up vessel 2 is drawn into the entrance of culture tank 7 medium-height trestles 8 by peristaltic pump 6 by conduit, see through support 8, flow in culture tank 7, nutrient solution in culture tank 7 is extracted out by peristaltic pump 4 by conduit from culture tank 7, by T-valve 5, is back to nutrient solution hold-up vessel 2 or flows into waste liquid hold-up vessel 1 from conduit 3.The gas that contains oxygen is added in nutrient solution from airway 9, and the waste gas in nutrient solution also can be discharged by airway 9.Support 8 mostly is porous media material, is planted in the cell in support 8 and flows through between the nutrient solution of support to have nutritive substance and metabolic excremental exchange process.Cell can be bred on a large scale in reactor, also can and then carry out cytodifferentiation.
Cell is in vitro in culturing process, need metastable microenvironment, need again to carry out the dynamic environment of efficient exchange of substance, this is the demand of conflict simultaneously, the nutrient solution of existing filling type bioreactor circulates mode, cannot effectively take into account this contradiction demand; In bio-reactor, for cell provides, needing the oxygen dissolved that keeps certain in the nutrient solution of nutritive substance, need mixed gas from oxygen to filling in nutrient solution that contain for this reason, when this mixed gas of filling, there are some problems in existing filling type bioreactor.Be that existing filling type bioreactor mainly has the following disadvantages:
(1), existing filling type bioreactor is difficult to overcome the contradiction demand that nutrient solution exists in circulating process: from the required nutritive substance of cell and the excremental effective transmission of angle of its metabolism, need nutrient solution continuous circulation perfusion incessantly; Yet, microenvironment angle from Growth of Cells, need to maintain metastable microenvironment, if the conversion of the microenvironment of cell cultures is too fast, the positive growth factor of meeting diluting cells secretion, be unfavorable for cell proliferation (reference: Wang Tiantian, the progress of stem cell in vitro dimensional culture, [J] international laboratory medicine magazine, the 33rd the 6th phase of volume of March in 2012), for this reason, need nutrient solution to remain relatively stable.The conventional terms of settlement of existing filling type bioreactor is that dynamic cultivation combines with static cultivation, i.e. first standing cultivation for some time, in this process, nutrient solution does not circulate, cell is cultivated in the static microenvironment of macroscopic view, then circumfusion is cultivated for some time, starts nutrient solution circumfusion function, by circulate nutrient solution between culture tank and nutrient solution hold-up vessel, in dynamic cultivation cell, the nutrient solution in retort is changed to fresh nutrient solution.This two kinds of static state and dynamic cultivation process hocket.The solution of this routine, owing to there is the static cultivation of for some time, makes the effect and quality of cell cultures not high.
(2), existing filling type bioreactor is having problems containing on the dosing method of oxygen gas mixture.Existing filling type bioreactor is generally by airway 9, the mixed gas that contains oxygen (comprising oxygen, carbonic acid gas, nitrogen) to be added in the nutrient solution in nutrient solution hold-up vessel 2 in the nutrient solution hold-up vessel 2 in Fig. 1, the mode of this filling gas, make the dissolved oxygen rate in nutrient solution lower, distribute also even not, and easily cause the bubble producing to enter in culture tank 7 with nutrient solution, and bubble can produce destruction to cultured cells while breaking.
Summary of the invention
The utility model is at least one defect overcoming described in above-mentioned prior art, a kind of filling type bioreactor of the double-deck recycle system is provided, the contradiction needs of problems in culturing process, nutrient solution being circulated to solve cell, and can effectively improve the solubleness of oxygen and the uniformity coefficient of distribution thereof in nutrient solution, be conducive to the discharge of waste gas in nutrient solution, and can effectively eliminate bubble and enter cell cultures region.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is: a kind of filling type bioreactor of the double-deck recycle system, wherein, comprise culture tank, be located at the outer recycle system of culture tank outside, the interior loop system of being located at culture tank inside and gas loading system;
In this programme, by the outer recycle system, realize nutrient solution in culture tank and obtain and upgrade from outside nutrient solution storage; By interior loop system, realizing nutrient solution in culture tank circulates in culture tank inside.Gas joins in the air in teeter column by gas loading system, and discharge is not dissolved into the unnecessary gas of nutrient solution and the waste gas of cell metabolism excretion.
In bio-reactor, designed inside and outside double-deck circumfusion system, outer circumfusion system is guaranteed that the nutritive substance concentration of nutrient solution in culture tank maintains and is conducive in scope that cell carries out exchange of substance; Interior loop filling system has been realized the local circulation in fixed space.In interior loop system, the fluid driving of blade stirring and the subregion in cell cultures district have been adopted but the designing technique of coenecium, the liquid motion that makes cell cultures district is mild laminar flow, the mild laminar flow in part in this fixed space, the microenvironment composition that can guarantee Growth of Cells keeps relative stability, and can be conducive to again effectively carrying out of exchange of substance between cell and nutrient solution.
Concrete, the described outer recycle system comprises waste liquid hold-up vessel, nutrient solution hold-up vessel, T-valve, the first peristaltic pump, the second peristaltic pump, nutrient solution ingress pipe and nutrient solution delivery line, it is inner that nutrient solution ingress pipe one end connects culture tank, the other end connects the second peristaltic pump, and the second peristaltic pump connects nutrient solution hold-up vessel by conduit; It is inner that nutrient solution delivery line one end connects culture tank, and the other end connects the first peristaltic pump; T-valve comprises three outlets, and its first outlet connects the first peristaltic pump, and the second outlet connects nutrient solution hold-up vessel by conduit, and the 3rd outlet connects waste liquid hold-up vessel by conduit; After the outer recycle system starts, the power that the second peristaltic pump produces is transported to the fresh medium in nutrient solution hold-up vessel in culture tank from nutrient solution ingress pipe, simultaneously, the first peristaltic pump is transported to T-valve by the old nutrient solution in culture tank from nutrient solution delivery line, by controlling T-valve, the nutrient solution that can control in nutrient solution delivery line is got back in nutrient solution hold-up vessel, or is transported in waste liquid hold-up vessel.The described outer recycle system is used for realizing the intermittent cycle of nutrient solution,, according to the interval time of setting, by peristaltic pump, the nutrient solution in culture tank is carried out to integral replacing, and now interior loop can be carried out simultaneously, also can stop.
Described interior loop system comprises column plate, bottom conduction pipe, the teeter column being located in culture tank, the bottom of column plate is connected with the bottom of teeter column by bottom conduction pipe, interior loop system also comprise be fixed on the motor on culture tank top, the trace being connected with motor, with the fixing blade of trace, trace and blade are located at inside, teeter column; Described teeter column also has liquid outlet, liquid outlet and culture tank internal communication; Described interior loop system is used for realizing nutrient solution and in culture tank, carries out local continuous circulation, and now outer circulation stops, and under the effect of the blade stirring in teeter column of the nutrient solution in culture tank, carries out local partial circulating between column plate and teeter column.
By this inside and outside double-deck circumfusion system, can solve the contradiction needs of problems that cell cultures circulates nutrient solution.The skin circulation adopting can be upgraded the nutrient solution in culture tank, and in the nutrient solution after renewal, nutraceutical concentration is higher, and the excremental concentration of cell metabolism is lower, and this situation is conducive to the metabolic processes of cell and effectively carries out; After nutrient solution has upgraded, stop outer circulation, interior loop makes nutrient solution only between column plate and teeter column, carry out local circulation, the internal recycling of this local space, the somatomedin of emiocytosis is still rested among this local space, can to the growth of cell, not cause disadvantageous effect because of this excessive too fast dilution because of subconstiuent, simultaneously, this local circulation, also be conducive to upgrade pericellular microenvironment, to cell, bring nutritive substance, take away metabolic movement, be conducive to carrying out smoothly of cell metabolism procedure.When metabolic processes is carried out after certain hour, nutrient concentrations in culture tank is because of lower by Cell uptake concentration, and excretion after refuse concentration higher, while being unfavorable for the carrying out smoothly of the needed material exchange process of cell metabolism, again start the outer recycle system, the nutrient solution of culture tank is carried out to integral replacing, so repeatedly hocket, thereby can solve the contradiction needs of problems of cell to nutrient solution circulation, for cell provides the microenvironment that is beneficial to its metabolic process.
Described gas loading system comprises gas introduction tube, gas eduction tube, and culture tank outside is located in gas introduction tube one end, and the other end connects the bottom of teeter column; The top of connection teeter column, gas eduction tube one end, it is outside that the other end connects culture tank.
Concrete, bio-reactor also comprises column plate permanent joint, base and base fixed link, and culture tank opening part is provided with top cover, and column plate permanent joint one end is fixed on top cover, and the other end is column plate fixedly; Base fixed link one end is fixed on top cover, the other end firm banking, base support column plate.
Concrete, described teeter column comprises teeter column's base, is located at teeter column's urceolus and teeter column's inner core on teeter column's base; Trace and blade are located in teeter column's inner core, and gas eduction tube and liquid outlet are located at urceolus top, teeter column.
Concrete, on the circumference of described teeter column's base, having gas introduction port, gas introduction port connects gas introduction tube, and inner core bottom in teeter column's is provided with gas inlet, and gas inlet is communicated with gas introduction port.
Described inner core bottom, teeter column is also provided with nutrient solution entrance, and nutrient solution entrance is communicated with bottom conduction pipe.
After interior loop system starts, motor drives blade to carry out high speed rotating by trace, the blade of high speed rotating drives the nutrient solution in blade region upwards outwards to throw away fast, make blade lower zone because of the minimizing pressure drop of nutrient solution, thereby the nutrient solution in column plate region can be attracted to blade lower zone by bottom conduction pipe.Along with the high-speed rotary of blade is transferred the nutrient solution upwards outwards throwing away, by the arrival liquid outlet that moves up of the inner-wall surface along teeter column's inner core, teeter column's urceolus, from liquid outlet, flow out teeter column, flow back into column plate region, the route that the nutrient solution of a culture tank inside of formation circulates.
The nutrient solution turbulent flow that blade high-speed stirring produces is after the obstruction shock absorption of teeter column's inner core, when the liquid outlet of urceolus top, Zai Cong teeter column flows out, it has been mild mobile laminar flow, thereby can avoid the impact of quick complicated turbulent flow on column plate region, the liquid motion that makes column plate region is simple laminar flow, and this is conducive to the relatively stable of Growth of Cells microenvironment in column plate region.
The nutrient solution of the mixed gas that contains oxygen (containing oxygen, carbonic acid gas, nitrogen) blade side-lower in gas introduction tube enters teeter column, the bubble that gas produces in nutrient solution, blade moment by high speed rotating smashes, and under the stirring action of blade, be dissolved in nutrient solution fast and equably.Undissolved unnecessary gas floats to top, teeter column, from gas eduction tube, discharges.After cell is excreted to waste gas in nutrient solution and enters in teeter column with nutrient solution, under the rapid stirring effect of blade, can from nutrient solution, discharge fast, then from gas eduction tube, discharge.
Trace drives blade to make high speed rotating under the driving of motor, nutrient solution in teeter column's inner core is produced to raising force, pull the nutrient solution in the conduction pipe of bottom to enter teeter column's inner core from nutrient solution entrance, realized the driving function of nutrient solution in culture tank being carried out to local circulation; Gas enters gas introduction port, from gas inlet, arrives the nutrient solution of side-lower of blade, and the bubble producing can be smashed by atwirl blade, thereby has realized the function of abolishing bubble; Enter into the gas of nutrient solution, under the stirring action of blade, can be dissolved into fast and equably in nutrient solution, realized oxygen and in nutrient solution, dissolved fast and equably function; Waste gas in nutrient solution under the stirring action of blade, can discharge separation rapidly from nutrient solution, has realized the release separation function that promotes waste gas in nutrient solution.
At the bottom, teeter column of interior loop system, the side-lower of blade, add gas.The bubble that the gas adding produces in nutrient solution, under the blade stirring effect of high speed rotating, can be smashed by moment.The centrifugal force that blade produces the material of different specific weight when high speed rotating is different, due to the proportion of specific gravity of gas much smaller than nutrient solution, nutrient solution can disperse to the periphery under the effect of larger centrifugal force, and the bubble not smashed in time on a small quantity can be under the effect of less centrifugal force, concentrate on region, vortex core and rapid and nutrient solution subregion distribution, then emersion liquid level makes progress, from the gas eduction tube of teeter column, discharge, thereby effectively eliminated bubble, enter while breaking behind cell cultures region the cell problem that causes damage.After bubble is crashed to pieces, under the effect of high-speed stirring, can be dissolved into fast in nutrient solution, improve the solubleness of oxygen in nutrient solution, and can be distributed in cultivation equably.And metabolic waste gas in nutrient solution, under the rapid stirring effect of blade, can be discharged on liquid level fast from nutrient solution, then from gas eduction tube, discharge.By this gas dosing method, can effectively improve the solubleness of oxygen and the uniformity coefficient of distribution thereof in nutrient solution, be conducive to the discharge of waste gas in nutrient solution, and can effectively eliminate bubble and enter cell cultures region.
Compared with prior art, beneficial effect is:
1, taken into account the conflict demand of cell cultures to the microenvironment of growth, that is: the somatomedin discharging for not too fast excess dilution cell, the microenvironment composition at cell place should remain relatively stable; For promoting the exchange of substance between cell and nutrient solution, the microenvironment at thin portion place should have certain variation, can supplement in time the cell cycle by the nutritive substance composition of Cell uptake, takes away the metabolite composition of cell excretion.
In bio-reactor, designed inside and outside double-deck circumfusion system, outer circumfusion system is guaranteed that the nutritive substance concentration of nutrient solution in culture tank maintains and is conducive in scope that cell carries out exchange of substance; Interior loop filling system has been realized the local circulation in fixed space.In interior loop system, the fluid driving of blade stirring and the subregion in cell cultures district have been adopted but the designing technique of coenecium, the liquid motion that makes cell cultures district is mild laminar flow, the mild laminar flow in part in this fixed space, the microenvironment composition that can guarantee Growth of Cells keeps relative stability, and can be conducive to again effectively carrying out of exchange of substance between cell and nutrient solution.
2, taken into account oxygen-containing gas in nutrient solution fast, evenly filling, the elimination that filling produces bubble during gas, and effective discharge problem of waste gas in nutrient solution.
Annotate in the nutrient solution of blade rotary area in the bio-reactor interior loop filling system method of gas, by atwirl blade, realized oxygen-containing gas in nutrient solution fast, evenly filling, the elimination that filling produces bubble during gas, and effective discharge function of waste gas in nutrient solution.
Accompanying drawing explanation
Fig. 1 is existing filling type bioreactor overall schematic.
Fig. 2 is overall schematic of the present utility model.
Fig. 3 is local enlarged diagram of the present utility model.
Embodiment
Accompanying drawing, only for exemplary illustration, can not be interpreted as the restriction to this patent; For better explanation the present embodiment, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product; To those skilled in the art, in accompanying drawing some known configurations and explanation thereof may to omit be understandable.
As shown in Figure 2,3, a kind of filling type bioreactor of the double-deck recycle system, wherein, comprises culture tank 5, is located at the outer recycle system of culture tank 5 outsides, the interior loop system of being located at culture tank 5 inside and gas loading system;
The outer recycle system comprises waste liquid hold-up vessel 1, nutrient solution hold-up vessel 2, T-valve 3, the first peristaltic pump 4A, the second peristaltic pump 4B, nutrient solution ingress pipe 6 and nutrient solution delivery line 7, nutrient solution ingress pipe 6 one end connect culture tank 5 inside, the other end connects the second peristaltic pump 4B, and the second peristaltic pump 4B connects nutrient solution hold-up vessel 2 by conduit; Nutrient solution delivery line 7 one end connect culture tank 5 inside, and the other end connects the first peristaltic pump 4A; T-valve 3 comprises three outlets, and its first outlet connects the first peristaltic pump 4A, and the second outlet connects 2, the three outlets of nutrient solution hold-up vessel by conduit and connects waste liquid hold-up vessel 1 by conduit; After the outer recycle system starts, the power that the second peristaltic pump 4B produces is transported to the fresh medium in nutrient solution hold-up vessel 2 in culture tank 5 from nutrient solution ingress pipe 6, simultaneously, the first peristaltic pump 4A is transported to T-valve 3 by the old nutrient solution in culture tank 5 from nutrient solution delivery line 7, by controlling T-valve 3, the nutrient solution that can control in nutrient solution delivery line is got back in nutrient solution hold-up vessel 2, or is transported in waste liquid hold-up vessel 1.The described outer recycle system is used for realizing the intermittent cycle of nutrient solution,, according to the interval time of setting, by peristaltic pump, the nutrient solution in culture tank is carried out to integral replacing, and now interior loop can be carried out simultaneously, also can stop.
Interior loop system comprises column plate 9, bottom conduction pipe 12, the teeter column being located in culture tank 5, the bottom of column plate 9 connects by the bottom of conduction pipe 12Yu teeter column, bottom, interior loop system also comprise be fixed on the motor 19 on culture tank 5 tops, the trace 20 being connected with motor 19, with the fixing blade 18 of trace 20, trace 20 and blade 18 are located at inside, teeter column; Described teeter column also has liquid outlet 16, liquid outlet 16 and culture tank 5 internal communication; Bio-reactor also comprises column plate permanent joint 8, base 10 and base fixed link 11, and culture tank 5 opening parts are provided with top cover, and column plate permanent joint 8 one end are fixed on top cover, and the other end is column plate 9 fixedly; Base fixed link 11 one end are fixed on top cover, the other end firm banking 10, base 10 support column plates 9.Teeter column comprises teeter column's base 24, is located at urceolus 15He teeter column, the teeter column inner core 17 on teeter column's base 24; Trace 20 and blade 18 are located in teeter column's inner core 17, and gas eduction tube 14 and liquid outlet 16 are located at teeter column's urceolus 15 tops.Gas loading system comprises gas introduction tube 13, gas eduction tube 14, and culture tank 5 outsides are located in gas introduction tube 13 one end, and the other end connects the bottom of teeter column; The top of gas eduction tube 14 connection teeter columns, one end, the other end connects culture tank 5 outsides.
After interior loop system starts, motor 19 drives blade 18 to carry out high speed rotating by trace 20, the blade 18 of high speed rotating drives the nutrient solution in blade region upwards outwards to throw away fast, make blade lower zone because of the minimizing pressure drop of nutrient solution, thereby the nutrient solution in column plate 9 regions can be attracted to blade lower zone by bottom conduction pipe 12.Along with the high-speed rotary of blade 18 is transferred the nutrient solution upwards outwards throwing away, inner-wall surface along teeter column's inner core, teeter column's urceolus is moved up and arrives liquid outlet 16, from liquid outlet 16, flow out teeter column, flow back into column plate 9 regions, the route that the nutrient solution of a culture tank inside of formation circulates.
The nutrient solution turbulent flow that blade 18 high-speed stirring produce is after the obstruction shock absorption of teeter column's inner core, when the liquid outlet 16 of urceolus top, Zai Cong teeter column flows out, it has been mild mobile laminar flow, thereby can avoid the impact of quick complicated turbulent flow on column plate 9 regions, the liquid motion that makes column plate 9 regions is simple laminar flow, and this is conducive to the relatively stable of Growth of Cells microenvironment in column plate 9 regions.
On the circumference of teeter column's base 24, have gas introduction port 21, gas introduction port 21 connects gas introduction tube 13, and inner core 17 bottoms in teeter column's are provided with gas inlet 22, and gas inlet 22 is communicated with gas introduction port 21.Inner core 17 bottoms in teeter column's are also provided with nutrient solution entrance 23, and nutrient solution entrance 23 is communicated with bottom conduction pipe 12.
The mixed gas that contains oxygen (containing oxygen, carbonic acid gas, nitrogen) enters the nutrient solution of blade side-lower in teeter column from gas introduction tube 13, the bubble that gas produces in nutrient solution, 18 moments of blade by high speed rotating smash, and under the stirring action of blade, be dissolved in nutrient solution fast and equably.Undissolved unnecessary gas floats to top, teeter column, from gas eduction tube 14, discharges.After cell is excreted to waste gas in nutrient solution and enters in teeter column with nutrient solution, under the rapid stirring effect of blade 18, can from nutrient solution, discharge fast, then from gas eduction tube 14, discharge.
Obviously, above-described embodiment of the present utility model is only for the utility model example is clearly described, and is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without also giving all embodiments.All any modifications of doing within spirit of the present utility model and principle, be equal to and replace and improvement etc., within all should being included in the protection domain of the utility model claim.
Claims (5)
1. a filling type bioreactor for the double-deck recycle system, is characterized in that, comprises culture tank (5), is located at the outside outer recycle system of culture tank (5), is located at culture tank (5) inner interior loop system and gas loading system;
The described outer recycle system comprises waste liquid hold-up vessel (1), nutrient solution hold-up vessel (2), T-valve (3), the first peristaltic pump (4A), the second peristaltic pump (4B), nutrient solution ingress pipe (6) and nutrient solution delivery line (7), nutrient solution ingress pipe (6) one end connects culture tank (5) inside, the other end connects the second peristaltic pump (4B), and the second peristaltic pump (4B) connects nutrient solution hold-up vessel (2) by conduit; Nutrient solution delivery line (7) one end connects culture tank (5) inside, and the other end connects the first peristaltic pump (4A); T-valve (3) comprises three outlets, and its first outlet connects the first peristaltic pump (4A), and the second outlet connects nutrient solution hold-up vessel (2) by conduit, and the 3rd outlet connects waste liquid hold-up vessel (1) by conduit;
Described interior loop system comprises column plate (9), bottom conduction pipe (12), the teeter column being located in culture tank (5), the bottom of column plate (9) is connected with the bottom of teeter column by bottom conduction pipe (12), interior loop system also comprise be fixed on the motor (19) on culture tank (5) top, the trace (20) being connected with motor (19), with the fixing blade (18) of trace (20), trace (20) and blade (18) are located at inside, teeter column; Described teeter column also has liquid outlet (16), liquid outlet (16) and culture tank (5) internal communication;
Described gas loading system comprises gas introduction tube (13), gas eduction tube (14), and culture tank (5) outside is located in gas introduction tube (13) one end, and the other end connects the bottom of teeter column; The top of connection teeter column, gas eduction tube (14) one end, the other end connects culture tank (5) outside.
2. the filling type bioreactor of a kind of double-deck recycle system according to claim 1, it is characterized in that, bio-reactor also comprises column plate permanent joint (8), base (10) and base fixed link (11), culture tank (5) opening part is provided with top cover, column plate permanent joint (8) one end is fixed on top cover, and the other end is column plate (9) fixedly; Base fixed link (11) one end is fixed on top cover, the other end firm banking (10), base (10) support column plate (9).
3. the filling type bioreactor of a kind of double-deck recycle system according to claim 2, it is characterized in that, described teeter column comprises teeter column's base (24), is located at teeter column's urceolus (15) and teeter column's inner core (17) on teeter column's base (24); Trace (20) and blade (18) are located in teeter column's inner core (17), and gas eduction tube (14) and liquid outlet (16) are located at teeter column's urceolus (15) top.
4. the filling type bioreactor of a kind of double-deck recycle system according to claim 3, it is characterized in that, on the circumference of described teeter column's base (24), have gas introduction port (21), gas introduction port (21) connects gas introduction tube (13), teeter column's inner core (17) bottom is provided with gas inlet (22), and gas inlet (22) are communicated with gas introduction port (21).
5. the filling type bioreactor of a kind of double-deck recycle system according to claim 4, is characterized in that, described teeter column's inner core (17) bottom is also provided with nutrient solution entrance (23), and nutrient solution entrance (23) is communicated with bottom conduction pipe (12).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320289836.9U CN203487159U (en) | 2013-05-24 | 2013-05-24 | Perfusion-type bioreactor with two layers of circulating systems |
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| CN201320289836.9U CN203487159U (en) | 2013-05-24 | 2013-05-24 | Perfusion-type bioreactor with two layers of circulating systems |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243027A (en) * | 2013-05-24 | 2013-08-14 | 中山大学 | Perfusion type bioreactor of double-layer circulating system and application method of perfusion type bioreactor |
| CN112625902A (en) * | 2020-12-03 | 2021-04-09 | 广州迈普再生医学科技股份有限公司 | Bioreactor and have its biological reaction system |
| CN114149917A (en) * | 2021-11-15 | 2022-03-08 | 安徽骆华生物科技有限公司 | Integrated living cell workstation |
-
2013
- 2013-05-24 CN CN201320289836.9U patent/CN203487159U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243027A (en) * | 2013-05-24 | 2013-08-14 | 中山大学 | Perfusion type bioreactor of double-layer circulating system and application method of perfusion type bioreactor |
| CN103243027B (en) * | 2013-05-24 | 2014-07-09 | 中山大学 | Perfusion type bioreactor of double-layer circulating system and application method of perfusion type bioreactor |
| CN112625902A (en) * | 2020-12-03 | 2021-04-09 | 广州迈普再生医学科技股份有限公司 | Bioreactor and have its biological reaction system |
| CN112625902B (en) * | 2020-12-03 | 2024-02-13 | 广州迈普再生医学科技股份有限公司 | Bioreactor and biological reaction system with same |
| CN114149917A (en) * | 2021-11-15 | 2022-03-08 | 安徽骆华生物科技有限公司 | Integrated living cell workstation |
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